MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 187)


Page: Previous 1 2 3 4 5 6 7  19 Next 

+11

Accession Score Description
1 RL11_PSEP1 297 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 SUCC_PSEPK 292 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+13

Accession Score Description
1 EFG1_PSEPK 288 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+14

Accession Score Description
1 ADHP_ECOLI 282 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+15

Accession Score Description
1 PORF_PSESY 281 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+16

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 276 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 181 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

-17

Accession Score Description
1 RS5_PSEE4 265 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
Score Mass Matches Sequences emPAI
17.1 RS5_PSEE4 265 17700 10 (7) 5 (3) 0.46
30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
4 samesets of RS5_PSEE4
RS5_PSEP1 265 17712 10 (7) 5 (3) 0.45
30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1
RS5_PSEPG 265 17712 10 (7) 5 (3) 0.45
30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1
RS5_PSEPK 265 17712 10 (7) 5 (3) 0.45
30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1
RS5_PSEPW 265 17712 10 (7) 5 (3) 0.45
30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1

-10 peptide matches (5 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
548 +1 364.7256 727.4366 727.4341 3.55 0 26 0.11 +1Score > 39 indicates identity
Score > 29 indicates homology
K.LVQVNR.V
812   428.2505 854.4864 854.4861 0.35 1 23 0.11 +1Score > 37 indicates identity
Score > 25 indicates homology
R.EVPAAIQK.A
1117 +1 491.7662 981.5178 981.5131 4.83 0 38 0.045 +1Score > 39 indicates identity
Score > 37 indicates homology
U K.GTTLQYATK.A
2101 +1 705.9272 1409.8398 1409.8242 11.1 1 64 3.9e-005 +1Score > 33 indicates identity U R.AVLEVAGVQNVLAK.C
2206 +2 734.9156 1467.8166 1467.7974 13.1 0 105 8.4e-009 +1Score > 37 indicates identity
Score > 36 indicates homology
U R.IFTFTALTVVGDGK.G

3 subsets and intersections (15 subset proteins in total)

Score Mass Subset of
RS5_AZOVD 257 17763 17.1
30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1
7 samesets of RS5_AZOVD
RS5_PSE14 257 17881
30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1
RS5_PSEF5 257 17699
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1
RS5_PSEFS 257 17732
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1
RS5_PSEMY 257 17708
30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1
RS5_PSEPF 257 17769
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1
RS5_PSESM 257 17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1
RS5_PSEU2 257 17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1
RS5_PSEU5 207 17649 17.1
30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1
RS5_ALCBS 81 17601 17.1
30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1
5 samesets of RS5_ALCBS
RS5_PSEA7 81 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1
RS5_PSEA8 81 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1
RS5_PSEAB 81 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1
RS5_PSEAE 81 17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1
RS5_HAHCH 81 17639
30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 258 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 RPOA_PSEPK 249 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+20

Accession Score Description
1 RL18_PSEP1 222 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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