MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 187)

Page: Previous 1 2 3 4 5 6 7 … 19 Next 

+11

Accession Score Description
1 RL11_PSEP1 297 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 SUCC_PSEPK 292 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+13

Accession Score Description
1 EFG1_PSEPK 288 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+14

Accession Score Description
1 ADHP_ECOLI 282 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+15

Accession Score Description
1 PORF_PSESY 281 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

-16

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 276 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 181 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RL4_PSEE4 276 21825 13 (6) 7 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
4 samesets of RL4_PSEE4
RL4_PSEP1 276 21798 13 (6) 7 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
RL4_PSEPG 276 21798 13 (6) 7 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas putida (strain GB-1) GN=rplD PE=3 SV=1
RL4_PSEPK 276 21798 13 (6) 7 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1
RL4_PSEPW 276 21798 13 (6) 7 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas putida (strain W619) GN=rplD PE=3 SV=1
RL4_PSE14 181 21901 12 (5) 6 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 samesets of RL4_PSE14
RL4_PSESM 181 21928 12 (5) 6 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas syringae pv. tomato GN=rplD PE=3 SV=1
RL4_PSEU2 181 21928 12 (5) 6 (4) 0.50
50S ribosomal protein L4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplD PE=3 SV=1

-15 peptide matches (10 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
341   314.6828 627.3510 627.3493 2.83 0 29 0.16 +1Score > 34 indicates identity
Score > 33 indicates homology		 X R.GPIWR.G
917 +1 450.7832 899.5518 899.5440 8.73 1 54 0.0014 +1Score > 37 indicates identity U X X R.SILAELVR  
918   900.5593 899.5520 899.5440 8.93 1 18 2.4 +9Score > 37 indicates identity
Score > 34 indicates homology		 U X X R.SILAELVR  
988 +1 465.3075 928.6004 928.5957 5.09 0 40 0.0064 +1Score > 30 indicates identity U X X K.VLITVSAVK.K
1035   475.2656 948.5166 948.5141 2.67 0 8 1.4 +3Score > 40 indicates identity
Score > 22 indicates homology		 X X R.NLPHVDVR.D
1036   317.1796 948.5170 948.5141 3.01 0 16 0.21 +1Score > 40 indicates identity
Score > 22 indicates homology		 X X R.NLPHVDVR.D
2112 +1 707.9078 1413.8010 1413.7868 10.1 1 74 1.1e-006 +1Score > 37 indicates identity
Score > 27 indicates homology		 U X X R.LVVVQDFAVEAPK.T
2567 +1 810.9166 1619.8186 1619.8043 8.88 1 98 1.9e-009 +1Score > 40 indicates identity
Score > 23 indicates homology		 U X R.DVQGSDPVSLIAYEK.V
3166   660.9832 1979.9278 1979.9411 -6.73 1 4 1.6 +6Score > 39 indicates identity
Score > 18 indicates homology		 U X X E.FNETLVHQAVVAYMAGGR.Q + 2 Deamidated (NQ); Oxidation (M)
4055 +1 883.4800 2647.4182 2647.3891 11.0 0 56 9.1e-006 +1Score > 34 indicates identity
Score > 18 indicates homology		 U X K.LTGMGLTDVLIVSDAVDQNLYLAAR.N

3 subsets and intersections (9 subset proteins in total)

Score Mass Subset of
RL4_PSEMY 177 21543 16.1
50S ribosomal protein L4 OS=Pseudomonas mendocina (strain ymp) GN=rplD PE=3 SV=1
RL4_PSEF5 60 21936 16.1
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEF5
RL4_PSEU5 60 21685
50S ribosomal protein L4 OS=Pseudomonas stutzeri (strain A1501) GN=rplD PE=3 SV=1
RL4_PSEFS 60 21987
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain SBW25) GN=rplD PE=3 SV=1
RL4_PSEPF 60 22005
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplD PE=3 SV=1
RL4_PSEA7 54 21627 16.1
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain PA7) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEA7
RL4_PSEA8 54 21600
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplD PE=3 SV=1
RL4_PSEAB 54 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplD PE=3 SV=1
RL4_PSEAE 54 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa GN=rplD PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEE4 265 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 258 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 RPOA_PSEPK 249 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+20

Accession Score Description
1 RL18_PSEP1 222 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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