MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 187)

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+151

Accession Score Description
1 NRG1_HUMAN 33 Pro-neuregulin-1, membrane-bound isoform OS=Homo sapiens GN=NRG1 PE=1 SV=3

+152

Accession Score Description
1 MFD_STAEQ 32 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

+153

Accession Score Description
1 SYL_SHEDO 32 Leucyl-tRNA synthetase OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=leuS PE=3 SV=2

+154

Accession Score Description
1 HFQ_PSEPK 32 Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1

+155

Accession Score Description
1 ARPB_PSEPU 32 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+156

Accession Score Description
1 IDH_SYNY3 32 Isocitrate dehydrogenase [NADP] OS=Synechocystis sp. (strain PCC 6803) GN=icd PE=1 SV=2

-157

Accession Score Description
1 RS1_PSEAE 31 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
Score Mass Matches Sequences emPAI
157.1 RS1_PSEAE 31 61946 13 (3) 6 (1) 0.04
30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
7 samesets of RS1_PSEAE
RS1_PROSP 31 41702 12 (3) 5 (1) 0.06
30S ribosomal protein S1 (Fragment) OS=Providencia sp. GN=rpsA PE=3 SV=1
RS1_BUCAI 31 62849 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rpsA PE=3 SV=1
RS1_BUCAP 31 62605 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsA PE=3 SV=1
RS1_ECO57 31 61235 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O157:H7 GN=rpsA PE=3 SV=1
RS1_ECOL6 31 61235 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O6 GN=rpsA PE=3 SV=1
RS1_ECOLI 31 61235 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
RS1_SHIFL 31 61235 10 (3) 4 (1) 0.04
30S ribosomal protein S1 OS=Shigella flexneri GN=rpsA PE=3 SV=1

-13 peptide matches (8 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
331 +1 308.7061 615.3976 615.3956 3.40 0 28 0.14 +1Score > 42 indicates identity
Score > 32 indicates homology		 R.VSLGLK.Q
351 +2 315.7138 629.4130 629.4112 2.95 0 29 0.32 +1Score > 38 indicates identity
Score > 36 indicates homology		 R.ISLGIK.Q
671   394.2262 786.4378 786.4348 3.88 0 30 0.06 +1Score > 44 indicates identity
Score > 30 indicates homology		 R.NNVVVSR.R
914   300.8195 899.4367 899.4349 2.01 1 17 1.2 +3Score > 39 indicates identity
Score > 30 indicates homology		 U R.DTTHLEGK.E
915   450.7263 899.4380 899.4349 3.54 1 19 0.46 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.DTTHLEGK.E
1441   567.8015 1133.5884 1133.5829 4.85 0 21 0.17 +1Score > 41 indicates identity
Score > 26 indicates homology		 U K.GGFTVDVNGIR.A
2340 +2 509.3049 1524.8929 1524.8777 9.98 0 28 0.014 +1Score > 33 indicates identity
Score > 22 indicates homology		 U R.AFLPGSLVDVRPVR.D
2342   763.4551 1524.8956 1524.8777 11.8 0 24 0.14 +1Score > 33 indicates identity
Score > 28 indicates homology		 U R.AFLPGSLVDVRPVR.D

+158

Accession Score Description
1 MUC16_HUMAN 31 Mucin-16 OS=Homo sapiens GN=MUC16 PE=1 SV=2

+159

Accession Score Description
1 ADH2_ENTHI 31 Aldehyde-alcohol dehydrogenase 2 OS=Entamoeba histolytica GN=ADH2 PE=1 SV=1

+160

Accession Score Description
1 NDK_PSEFS 30 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1
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