MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 187)


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+151

Accession Score Description
1 NRG1_HUMAN 33 Pro-neuregulin-1, membrane-bound isoform OS=Homo sapiens GN=NRG1 PE=1 SV=3

-152

Accession Score Description
1 MFD_STAEQ 32 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1
Score Mass Matches Sequences emPAI
152.1 MFD_STAEQ 32 134802 3 (1) 1 (1) 0.02
Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1
1 sameset of MFD_STAEQ
MFD_STAES 32 134832 3 (1) 1 (1) 0.02
Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 12228) GN=mfd PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1670   408.5602 1222.6588 1222.6557 2.50 1 32 0.07 +1Score > 39 indicates identity
Score > 33 indicates homology
U E.VIQHLQNELK.K + 2 Deamidated (NQ)
1672   408.5609 1222.6609 1222.6557 4.22 1 19 0.34 +1Score > 39 indicates identity
Score > 27 indicates homology
U E.VIQHLQNELK.K + 2 Deamidated (NQ)
1673   612.3380 1222.6614 1222.6557 4.69 1 32 0.03 +1Score > 39 indicates identity
Score > 30 indicates homology
U E.VIQHLQNELK.K + 2 Deamidated (NQ)

+153

Accession Score Description
1 SYL_SHEDO 32 Leucyl-tRNA synthetase OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=leuS PE=3 SV=2

+154

Accession Score Description
1 HFQ_PSEPK 32 Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1

+155

Accession Score Description
1 ARPB_PSEPU 32 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+156

Accession Score Description
1 IDH_SYNY3 32 Isocitrate dehydrogenase [NADP] OS=Synechocystis sp. (strain PCC 6803) GN=icd PE=1 SV=2

+157

Accession Score Description
1 RS1_PSEAE 31 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1

+158

Accession Score Description
1 MUC16_HUMAN 31 Mucin-16 OS=Homo sapiens GN=MUC16 PE=1 SV=2

+159

Accession Score Description
1 ADH2_ENTHI 31 Aldehyde-alcohol dehydrogenase 2 OS=Entamoeba histolytica GN=ADH2 PE=1 SV=1

+160

Accession Score Description
1 NDK_PSEFS 30 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1
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