MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 187)

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+141

Accession Score Description
1 IML1_MAGGR 36 description

+142

Accession Score Description
1 PANC_SHEON 36 Pantothenate synthetase OS=Shewanella oneidensis GN=panC PE=3 SV=1

+143

Accession Score Description
1 PIFA_ECOLI 36 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

-144

Accession Score Description
1 RS8_PSEP1 34 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
Score Mass Matches Sequences emPAI
144.1 RS8_PSEP1 34 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
13 samesets of RS8_PSEP1
RS8_PSEPG 34 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain GB-1) GN=rpsH PE=3 SV=1
RS8_PSEPK 34 14023 3 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain KT2440) GN=rpsH PE=3 SV=1
RS8_PSEE4 34 14024 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas entomophila (strain L48) GN=rpsH PE=3 SV=1
RS8_PSEF5 34 14178 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsH PE=3 SV=1
RS8_PSEFS 34 14140 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsH PE=3 SV=1
RS8_PSEMY 34 14106 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas mendocina (strain ymp) GN=rpsH PE=3 SV=1
RS8_PSEPF 34 14166 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsH PE=3 SV=1
RS8_PSEPW 34 14039 2 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain W619) GN=rpsH PE=3 SV=1
RS8_PSEA7 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsH PE=3 SV=2
RS8_PSEA8 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsH PE=3 SV=1
RS8_PSEAB 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsH PE=3 SV=1
RS8_PSEAE 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa GN=rpsH PE=3 SV=1
RS8_PSEU5 34 14047 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas stutzeri (strain A1501) GN=rpsH PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
200   487.3276 486.3203 486.3166 7.71 0 26 2.4 +4Score > 46 indicates identity
Score > 43 indicates homology		 K.VAVAK.V
572   372.2196 742.4246 742.4225 2.89 0 15 1.9 +3Score > 41 indicates identity
Score > 30 indicates homology		 U K.AVTDLPK.V
1434   566.3245 1130.6344 1130.6295 4.33 0 34 0.016 +1Score > 39 indicates identity
Score > 29 indicates homology		 U R.GGLGVSIVSTNK.G

+145

Accession Score Description
1 LCP5_YEAST 34 U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1

+146

Accession Score Description
1 ODO2_PSEPU 34 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

+147

Accession Score Description
1 TRYP_PIG 33 Trypsin OS=Sus scrofa PE=1 SV=1

+148

Accession Score Description
1 CAPZA_DROME 33 F-actin-capping protein subunit alpha OS=Drosophila melanogaster GN=cpa PE=1 SV=1

+149

Accession Score Description
1 RS6_BORA1 33 30S ribosomal protein S6 OS=Bordetella avium (strain 197N) GN=rpsF PE=3 SV=1

+150

Accession Score Description
1 STHA_PSEPG 33 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1
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