MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 187)


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+141

Accession Score Description
1 IML1_MAGGR 36 description

+142

Accession Score Description
1 PANC_SHEON 36 Pantothenate synthetase OS=Shewanella oneidensis GN=panC PE=3 SV=1

-143

Accession Score Description
1 PIFA_ECOLI 36 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2
Score Mass Matches Sequences emPAI
143.1 PIFA_ECOLI 36 85141 2 (1) 2 (1) 0.03
Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
535   723.3616 722.3543 722.3520 3.17 0 4 1.1 +9Score > 41 indicates identity
Score > 17 indicates homology
R.TITLME.K + Oxidation (M)
1256   521.8409 1041.6672 1041.6546 12.1 0 36 0.014 +1Score > 30 indicates identity U K.TSLLNLILR.N

+144

Accession Score Description
1 RS8_PSEP1 34 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+145

Accession Score Description
1 LCP5_YEAST 34 U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1

+146

Accession Score Description
1 ODO2_PSEPU 34 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

+147

Accession Score Description
1 TRYP_PIG 33 Trypsin OS=Sus scrofa PE=1 SV=1

+148

Accession Score Description
1 CAPZA_DROME 33 F-actin-capping protein subunit alpha OS=Drosophila melanogaster GN=cpa PE=1 SV=1

+149

Accession Score Description
1 RS6_BORA1 33 30S ribosomal protein S6 OS=Bordetella avium (strain 197N) GN=rpsF PE=3 SV=1

+150

Accession Score Description
1 STHA_PSEPG 33 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1
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