MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 187)


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-141

Accession Score Description
1 IML1_MAGGR 36 description
Score Mass Matches Sequences emPAI
141.1 IML1_MAGGR 36 0 3 (1) 3 (1) 0.01
description
12 samesets of IML1_MAGGR
L_SV5 36 0 2 (1) 2 (1) 0.01
description
L_SV5WR 36 0 2 (1) 2 (1) 0.01
description
EGH_DROME 36 0 1 (1) 1 (1) 0.05
description
PYRE_PYRIL 36 0 1 (1) 1 (1) 0.11
description
RIBA_MANSM 36 0 1 (1) 1 (1) 0.10
description
RIBA_THEVO 36 0 1 (1) 1 (1) 0.11
description
RUVB_DESAH 36 0 1 (1) 1 (1) 0.06
description
RUVB_DESOH 36 0 1 (1) 1 (1) 0.06
description
NUON1_HERA2 36 0 1 (1) 1 (1) 0.04
description
RIBA_HAES1 36 0 1 (1) 1 (1) 0.10
description
RIBA_HAES2 36 0 1 (1) 1 (1) 0.10
description
PES10_CAEEL 36 0 1 (1) 1 (1) 0.05
description

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
222   516.2924 515.2851 515.2816 6.90 0 21 4.3 +5Score > 40 indicates identity
Score > 39 indicates homology
R.AQAAR.D
362   320.1857 638.3568 638.3540 4.41 0 36 0.036 +1Score > 35 indicates identity
Score > 35 indicates homology
U E.FGIFR.R
520   358.7018 715.3890 715.3752 19.4 1 7 1.5 +9Score > 44 indicates identity
Score > 21 indicates homology
U E.LTEEPK.T

+142

Accession Score Description
1 PANC_SHEON 36 Pantothenate synthetase OS=Shewanella oneidensis GN=panC PE=3 SV=1

+143

Accession Score Description
1 PIFA_ECOLI 36 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

+144

Accession Score Description
1 RS8_PSEP1 34 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+145

Accession Score Description
1 LCP5_YEAST 34 U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1

+146

Accession Score Description
1 ODO2_PSEPU 34 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

+147

Accession Score Description
1 TRYP_PIG 33 Trypsin OS=Sus scrofa PE=1 SV=1

+148

Accession Score Description
1 CAPZA_DROME 33 F-actin-capping protein subunit alpha OS=Drosophila melanogaster GN=cpa PE=1 SV=1

+149

Accession Score Description
1 RS6_BORA1 33 30S ribosomal protein S6 OS=Bordetella avium (strain 197N) GN=rpsF PE=3 SV=1

+150

Accession Score Description
1 STHA_PSEPG 33 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1
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