MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 187)


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+131

Accession Score Description
1 SYA_DICDI 40 Alanyl-tRNA synthetase OS=Dictyostelium discoideum GN=alaS PE=2 SV=1

+132

Accession Score Description
1 CH602_RHOBA 39 60 kDa chaperonin 2 OS=Rhodopirellula baltica GN=groL2 PE=3 SV=1

+133

Accession Score Description
1 RS3_PSEE4 39 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1

-134

Accession Score Description
1 SECA2_STAA1 38 Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=secA2 PE=3 SV=1
Score Mass Matches Sequences emPAI
134.1 SECA2_STAA1 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=secA2 PE=3 SV=1
9 samesets of SECA2_STAA1
SECA2_STAA3 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain USA300) GN=secA2 PE=3 SV=1
SECA2_STAA8 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain NCTC 8325) GN=secA2 PE=3 SV=1
SECA2_STAAC 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain COL) GN=secA2 PE=3 SV=1
SECA2_STAAE 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain Newman) GN=secA2 PE=3 SV=1
SECA2_STAAM 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=secA2 PE=3 SV=1
SECA2_STAAN 38 91101 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain N315) GN=secA2 PE=3 SV=1
SECA2_STAAR 38 91219 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain MRSA252) GN=secA2 PE=3 SV=1
SECA2_STAAS 38 91071 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain MSSA476) GN=secA2 PE=3 SV=1
SECA2_STAAW 38 91071 5 (1) 1 (1) 0.02
Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain MW2) GN=secA2 PE=3 SV=1

-5 peptide matches (2 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1812 +1 640.3574 1278.7002 1278.6932 5.54 1 38 0.022 +1Score > 38 indicates identity
Score > 34 indicates homology
U K.LQAGLHQAIEAK.E + Deamidated (NQ)
1813 +2 427.2411 1278.7015 1278.6932 6.50 1 19 1.3 +2Score > 38 indicates identity
Score > 33 indicates homology
U K.LQAGLHQAIEAK.E + Deamidated (NQ)

+135

Accession Score Description
1 ACCC1_BACSU 38 Biotin carboxylase 1 OS=Bacillus subtilis GN=accC1 PE=3 SV=3

+136

Accession Score Description
1 YA95_SCHPO 37 Uncharacterized protein C13G6.05c OS=Schizosaccharomyces pombe GN=SPAC13G6.05c PE=1 SV=2

+137

Accession Score Description
1 BFR_ECOLI 37 Bacterioferritin OS=Escherichia coli (strain K12) GN=bfr PE=1 SV=1

+138

Accession Score Description
1 SECB_PSEPG 37 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+139

Accession Score Description
1 XYLE2_PSEPU 37 Metapyrocatechase OS=Pseudomonas putida GN=xylE PE=3 SV=1

+140

Accession Score Description
1 RAVA_ECOBW 37 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1
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