MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 187)


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+131

Accession Score Description
1 SYA_DICDI 40 Alanyl-tRNA synthetase OS=Dictyostelium discoideum GN=alaS PE=2 SV=1

+132

Accession Score Description
1 CH602_RHOBA 39 60 kDa chaperonin 2 OS=Rhodopirellula baltica GN=groL2 PE=3 SV=1

-133

Accession Score Description
1 RS3_PSEE4 39 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
Score Mass Matches Sequences emPAI
133.1 RS3_PSEE4 39 25709 13 (2) 7 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
3 samesets of RS3_PSEE4
RS3_PSEP1 39 25709 13 (2) 7 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsC PE=3 SV=1
RS3_PSEPK 39 25709 13 (2) 7 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
RS3_PSEU5 39 25730 13 (2) 7 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas stutzeri (strain A1501) GN=rpsC PE=3 SV=1

-13 peptide matches (10 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
393 +1 330.1963 658.3780 658.3762 2.78 0 32 0.16 +1Score > 44 indicates identity
Score > 36 indicates homology
K.IQVSGR.L
468   346.7122 691.4098 691.4057 5.95 0 18 0.47 +1Score > 37 indicates identity
Score > 27 indicates homology
K.VWIFK.G
591   377.6808 753.3470 753.3446 3.28 1 18 0.79 +1Score > 39 indicates identity
Score > 29 indicates homology
R.TEWYR.E
667 +1 393.7278 785.4410 785.4395 1.93 1 26 0.89 +1Score > 43 indicates identity
Score > 38 indicates homology
R.LGGAEIAR.T
1802   320.1831 1276.7033 1276.7000 2.58 0 7 1.2 +2Score > 38 indicates identity
Score > 20 indicates homology
U R.IDIHRPAQTAR.I
1803   639.3596 1276.7046 1276.7000 3.64 0 23 0.44 +1Score > 38 indicates identity
Score > 32 indicates homology
U R.IDIHRPAQTAR.I
1805   426.5760 1276.7062 1276.7000 4.84 0 18 0.1 +1Score > 38 indicates identity
Score > 21 indicates homology
U R.IDIHRPAQTAR.I
2230 +1 369.7333 1474.9041 1474.8984 3.88 0 11 0.093 +1Score > 26 indicates identity
Score > 13 indicates homology
U R.ITIHTARPGIVIGK.K
2231   492.6429 1474.9069 1474.8984 5.76 0 22 0.018 +1Score > 25 indicates identity
Score > 17 indicates homology
U R.ITIHTARPGIVIGK.K
3599   744.3675 2230.0807 2230.0430 16.9 1 36 0.0022 +1Score > 39 indicates identity
Score > 22 indicates homology
U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

1 subset or intersection (19 subset proteins in total)

Score Mass Subset of
RS3_CHRSD 22 26097 133.1
30S ribosomal protein S3 OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpsC PE=3 SV=1
18 samesets of RS3_CHRSD
RS3_PSE14 22 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsC PE=3 SV=1
RS3_PSEA7 22 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsC PE=3 SV=1
RS3_PSEAB 22 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsC PE=3 SV=1
RS3_PSEAE 22 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa GN=rpsC PE=3 SV=1
RS3_PSEF5 22 25676
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsC PE=3 SV=1
RS3_PSEPF 22 25737
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsC PE=3 SV=1
RS3_PSESM 22 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. tomato GN=rpsC PE=3 SV=1
RS3_PSEU2 22 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsC PE=3 SV=1
RS3_PSEMY 22 25525
30S ribosomal protein S3 OS=Pseudomonas mendocina (strain ymp) GN=rpsC PE=3 SV=1
RS3_HAHCH 22 25467
30S ribosomal protein S3 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsC PE=3 SV=1
RS3_MARAV 22 25498
30S ribosomal protein S3 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rpsC PE=3 SV=1
RS3_FRATF 22 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsC PE=3 SV=1
RS3_FRATH 22 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain LVS) GN=rpsC PE=3 SV=1
RS3_FRATM 22 24848
30S ribosomal protein S3 OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=rpsC PE=3 SV=1
RS3_FRATN 22 24792
30S ribosomal protein S3 OS=Francisella tularensis subsp. novicida (strain U112) GN=rpsC PE=3 SV=1
RS3_FRATO 22 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=rpsC PE=3 SV=1
RS3_FRATW 22 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=rpsC PE=3 SV=1
RS3_NITOC 22 24932
30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+134

Accession Score Description
1 SECA2_STAA1 38 Protein translocase subunit secA 2 OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=secA2 PE=3 SV=1

+135

Accession Score Description
1 ACCC1_BACSU 38 Biotin carboxylase 1 OS=Bacillus subtilis GN=accC1 PE=3 SV=3

+136

Accession Score Description
1 YA95_SCHPO 37 Uncharacterized protein C13G6.05c OS=Schizosaccharomyces pombe GN=SPAC13G6.05c PE=1 SV=2

+137

Accession Score Description
1 BFR_ECOLI 37 Bacterioferritin OS=Escherichia coli (strain K12) GN=bfr PE=1 SV=1

+138

Accession Score Description
1 SECB_PSEPG 37 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+139

Accession Score Description
1 XYLE2_PSEPU 37 Metapyrocatechase OS=Pseudomonas putida GN=xylE PE=3 SV=1

+140

Accession Score Description
1 RAVA_ECOBW 37 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1
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