MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 187)


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+121

Accession Score Description
1 RL23_PSEE4 44 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1

+122

Accession Score Description
1 FKBP_DEBHA 43 FK506-binding protein 1 OS=Debaryomyces hansenii GN=FPR1 PE=3 SV=1

+123

Accession Score Description
1 SYD_HAEIE 43 Aspartyl-tRNA synthetase OS=Haemophilus influenzae (strain PittEE) GN=aspS PE=3 SV=1

+124

Accession Score Description
1 IF2_NOVAD 42 Translation initiation factor IF-2 OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=infB PE=3 SV=1

+125

Accession Score Description
1 SYR_EUBR3 42 Arginyl-tRNA synthetase OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=argS PE=3 SV=1

+126

Accession Score Description
1 MURA_PSEPG 41 UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=Pseudomonas putida (strain GB-1) GN=murA PE=3 SV=1

+127

Accession Score Description
1 GLO2_RHOPB 41 Hydroxyacylglutathione hydrolase OS=Rhodopseudomonas palustris (strain BisB18) GN=gloB PE=3 SV=1

+128

Accession Score Description
1 Y041_SYNY3 41 Putative methyl-accepting chemotaxis protein sll0041 OS=Synechocystis sp. (strain PCC 6803) GN=sll0041 PE=3 SV=2

+129

Accession Score Description
1 RS7_AZOVD 40 30S ribosomal protein S7 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsG PE=3 SV=1

-130

Accession Score Description
1 PHS_PSEAB 41 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1
Score Mass Matches Sequences emPAI
130.1 PHS_PSEAB 41 13439 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1
9 samesets of PHS_PSEAB
PHS_PSEAE 41 13439 1 (1) 1 (1) 0.18
Pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa GN=phhB PE=3 SV=1
PHS_PSEFS 41 13471 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas fluorescens (strain SBW25) GN=PFLU_4459 PE=3 SV=1
PHS_PSEPF 41 13459 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas fluorescens (strain Pf0-1) GN=Pfl01_1498 PE=3 SV=1
PHS_PSESM 41 13282 1 (1) 1 (1) 0.18
Pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas syringae pv. tomato GN=phhB PE=3 SV=1
PHS_PSEU2 41 13432 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=Psyr_3576 PE=3 SV=1
PHS_PSEF5 41 13427 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=PFL_1610 PE=3 SV=1
PHS_PSEA7 41 13481 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain PA7) GN=PSPA7_4645 PE=3 SV=1
PHS_PSEA8 41 13439 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain LESB58) GN=PLES_44451 PE=3 SV=1
PHS_PSE14 41 13224 1 (1) 1 (1) 0.18
Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=PSPPH_3532 PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1787   635.8322 1269.6498 1269.6353 11.4 1 41 0.0064 +1Score > 40 indicates identity
Score > 31 indicates homology
U R.QIPDWNIEVR.D + Deamidated (NQ)

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