MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 187)

Page: Previous 1 2 3 4 5 6 7 … 19 Next 

+11

Accession Score Description
1 RL11_PSEP1 297 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 SUCC_PSEPK 292 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

-13

Accession Score Description
1 EFG1_PSEPK 288 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 EFG1_PSEPK 288 79053 13 (10) 10 (7) 0.26
Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

-13 peptide matches (11 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
532   361.2169 720.4192 720.4170 3.09 0 7 1.2 +4Score > 38 indicates identity
Score > 21 indicates homology		 R.VVYVNK.M
582   374.7107 747.4068 747.4028 5.47 0 35 0.14 +1Score > 41 indicates identity
Score > 39 indicates homology		 U R.AGANFLR.V
1293   531.7645 1061.5144 1061.5142 0.27 1 56 0.0023 +1Score > 42 indicates identity U K.LAQEDPSFR.V
1554   393.5593 1177.6561 1177.6495 5.55 0 30 0.16 +1Score > 38 indicates identity
Score > 35 indicates homology		 R.ILFYTGLSHK.M
1859   650.8928 1299.7710 1299.7551 12.3 0 29 0.0034 +1Score > 34 indicates identity
Score > 17 indicates homology		 U K.NGVVAGYPLIGLK.A
2266 +1 748.4257 1494.8368 1494.8082 19.1 0 59 0.00027 +1Score > 36 indicates identity U R.GITITSAAVTTFWK.G
2361 +1 768.9351 1535.8556 1535.8348 13.6 0 91 1.7e-007 +1Score > 36 indicates identity U K.IATDPFVGTLTFVR.V
2415   521.5807 1561.7203 1561.7049 9.86 0 20 0.035 +1Score > 40 indicates identity
Score > 18 indicates homology		 U R.HADDDEPFSALAFK.I
2856   886.9657 1771.9168 1771.8992 9.93 0 52 5.7e-005 +1Score > 40 indicates identity
Score > 22 indicates homology		 U R.VYSGFLTSGDSVINSVK.G
2863   887.4660 1772.9174 1772.8833 19.3 0 60 2.3e-005 +1Score > 40 indicates identity
Score > 26 indicates homology		 U R.VYSGFLTSGDSVINSVK.G + Deamidated (NQ)
4153   926.1731 2775.4975 2775.4443 19.2 1 79 5e-008 +1Score > 33 indicates identity
Score > 19 indicates homology		 U R.LGHTPVPVQLAIGSEDNFQGQVDLIK.M + Deamidated (NQ)

+12 subsets and intersections (119 subset proteins in total)

+14

Accession Score Description
1 ADHP_ECOLI 282 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+15

Accession Score Description
1 PORF_PSESY 281 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+16

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 276 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 181 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEE4 265 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 258 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+19

Accession Score Description
1 RPOA_PSEPK 249 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+20

Accession Score Description
1 RL18_PSEP1 222 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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