MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 297 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 292 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | SUCC_PSEPK |
292 | 41499 | 20 (10) | 12 (7) | 0.46 | |
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 | |||||||
| 3 samesets of SUCC_PSEPK | |||||||
| SUCC_PSEP1 |
292 | 41499 | 20 (10) | 12 (7) | 0.46 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEPW |
292 | 41472 | 19 (10) | 11 (7) | 0.46 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEE4 |
292 | 41472 | 19 (10) | 11 (7) | 0.46 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1 | |||||||
20 peptide matches (12 non-duplicate, 8 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 404 |
1 |
334.7272 | 667.4398 | 667.4381 | 2.60 | 0 | 21 | 0.095 | 3Score > 29 indicates identity Score > 23 indicates homology |
K.VPVVVR.L | |
| 580 |
1 |
374.2436 | 746.4726 | 746.4691 | 4.81 | 0 | 40 | 0.011 | 1Score > 33 indicates identity |
U | K.IFVGLAK.L |
| 792 |
424.7361 | 847.4576 | 847.4552 | 2.89 | 0 | 38 | 0.092 | 1Score > 41 indicates identity Score > 40 indicates homology |
U | K.QVQQFAK.I | |
| 1132 |
2 |
496.2741 | 990.5336 | 990.5287 | 5.01 | 0 | 50 | 0.0003 | 1Score > 41 indicates identity Score > 27 indicates homology |
U | K.AFAAQWLGK.N |
| 1162 |
501.2764 | 1000.5382 | 1000.5342 | 4.07 | 1 | 16 | 0.34 | 1Score > 42 indicates identity Score > 24 indicates homology |
U | K.IGGNEWVVK.A | |
| 1444 |
1 |
567.8153 | 1133.6160 | 1133.6081 | 7.04 | 1 | 27 | 0.074 | 1Score > 40 indicates identity Score > 28 indicates homology |
K.ELYLGAVVDR.S | |
| 1830 |
430.1910 | 1287.5512 | 1287.5480 | 2.46 | 0 | 9 | 0.38 | 1Score > 37 indicates identity Score > 17 indicates homology |
U | K.TFHDPSQDDAR.E | |
| 1972 |
2 |
676.3773 | 1350.7400 | 1350.7183 | 16.1 | 1 | 74 | 7.5e-007 | 1Score > 38 indicates identity Score > 25 indicates homology |
U | K.QLFAEYGLPVSK.G |
| 2433 |
1 |
785.4323 | 1568.8500 | 1568.8311 | 12.1 | 0 | 84 | 4.3e-007 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | K.ATIDPLVGAQPFQGR.E |
| 2702 |
563.6648 | 1687.9726 | 1687.9509 | 12.9 | 1 | 45 | 0.00013 | 1Score > 32 indicates identity Score > 18 indicates homology |
U | K.DHDLALLEVNPLVIK.A | |
| 2804 |
868.4388 | 1734.8630 | 1734.8424 | 11.9 | 0 | 69 | 5.1e-006 | 1Score > 41 indicates identity Score > 29 indicates homology |
U | K.NLVTYQTDANGQPVSK.I + Deamidated (NQ) | |
| 3840 |
805.1257 | 2412.3553 | 2412.3111 | 18.3 | 1 | 33 | 0.039 | 1Score > 31 indicates identity |
U | K.VLAESGLNIIAATSLTDAAQQVVK.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SUCC_PSEPG |
226 | 41413 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEF5 |
192 | 41599 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEF5 | |||||
| SUCC_PSEFS |
192 | 41547 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSE14 |
185 | 41494 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_PSE14 | |||||
| SUCC_PSESM |
185 | 41472 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEU2 |
185 | 41442 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEA7 |
152 | 41772 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1 | |||||
| 3 samesets of SUCC_PSEA7 | |||||
| SUCC_PSEAB |
152 | 41830 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEAE |
152 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2 | |||||
| SUCC_PSEA8 |
152 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEPF |
135 | 41527 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_MARAV |
109 | 41495 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1 | |||||
| 4 samesets of SUCC_MARAV | |||||
| SUCC_PSEU5 |
109 | 41761 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEMY |
109 | 41759 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1 | |||||
| SUCC_SACD2 |
109 | 41524 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1 | |||||
| SUCC_TERTT |
109 | 41703 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBC3 |
69 | 41658 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_VIBC3 | |||||
| SUCC_VIBCH |
69 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBCM |
69 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1 | |||||
| SUCC_ALCBS |
40 | 41662 | 12.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=sucC PE=3 SV=1 | |||||
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 288 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 282 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 281 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEE4 | 276 | 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSE14 | 181 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 265 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 258 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 249 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 222 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
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