MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 187)


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+111

Accession Score Description
1 AT1A1_CATCO 46 Sodium/potassium-transporting ATPase subunit alpha-1 OS=Catostomus commersoni GN=atp1a1 PE=2 SV=1

+112

Accession Score Description
1 ATMB_SALTY 46 Magnesium-transporting ATPase, P-type 1 OS=Salmonella typhimurium GN=mgtB PE=2 SV=3

+113

Accession Score Description
1 ATP6_PSEP1 45 ATP synthase subunit a OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpB PE=3 SV=1

+114

Accession Score Description
1 SOML2_SPAAU 45 Somatolactin-2 OS=Sparus aurata PE=2 SV=1

+115

Accession Score Description
1 LEUC_PSEF5 45 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+116

Accession Score Description
1 RS1_HAEIN 45 30S ribosomal protein S1 OS=Haemophilus influenzae GN=rpsA PE=3 SV=1

+117

Accession Score Description
1 EFTU_ANATD 45 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

-118

Accession Score Description
1 RL19_PSEE4 44 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1
Score Mass Matches Sequences emPAI
118.1 RL19_PSEE4 44 12990 4 (2) 3 (2) 0.40
50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1
3 samesets of RL19_PSEE4
RL19_PSEP1 44 13004 4 (2) 3 (2) 0.40
50S ribosomal protein L19 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplS PE=3 SV=1
RL19_PSEPG 44 13004 4 (2) 3 (2) 0.40
50S ribosomal protein L19 OS=Pseudomonas putida (strain GB-1) GN=rplS PE=3 SV=1
RL19_PSEPK 44 13004 4 (2) 3 (2) 0.40
50S ribosomal protein L19 OS=Pseudomonas putida (strain KT2440) GN=rplS PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1077 +1 482.7675 963.5204 963.5138 6.92 0 16 0.11 +1Score > 41 indicates identity
Score > 19 indicates homology
R.GLNSAFTVR.K
2713   849.4474 1696.8802 1696.8672 7.69 0 37 0.00052 +1Score > 39 indicates identity
Score > 17 indicates homology
U R.TFQTYSPQIDSLAVK.R
2719   850.4804 1698.9462 1698.9193 15.9 1 29 0.04 +1Score > 36 indicates identity
Score > 28 indicates homology
U K.EIPTFAPGDTVVVQVK.V

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
RL19_PSEPW 37 13018 118.1
50S ribosomal protein L19 OS=Pseudomonas putida (strain W619) GN=rplS PE=3 SV=1
RL19_PSEMY 29 13048 118.1
50S ribosomal protein L19 OS=Pseudomonas mendocina (strain ymp) GN=rplS PE=3 SV=1

+119

Accession Score Description
1 S6A11_HUMAN 44 Sodium- and chloride-dependent GABA transporter 3 OS=Homo sapiens GN=SLC6A11 PE=2 SV=1

+120

Accession Score Description
1 DNBI_SCMVC 44 Major DNA-binding protein OS=Simian cytomegalovirus (strain Colburn) GN=UL57 PE=2 SV=2
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