MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–110 (out of 187)


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+101

Accession Score Description
1 CLPX_VARPS 50 ATP-dependent Clp protease ATP-binding subunit clpX OS=Variovorax paradoxus (strain S110) GN=clpX PE=3 SV=1

+102

Accession Score Description
1 RS18_PSEE4 50 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+103

Accession Score Description
1 GLNA_RHOCA 49 Glutamine synthetase (Fragment) OS=Rhodobacter capsulatus GN=glnA PE=3 SV=2

+104

Accession Score Description
1 CYOB_PSEPU 49 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+105

Accession Score Description
1 EFP_PSEPG 48 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+106

Accession Score Description
1 DNAB_MYCPN 48 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+107

Accession Score Description
1 ACCD_PSEF5 48 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=accD PE=3 SV=1

+108

Accession Score Description
1 ODP1_MYCTU 48 Pyruvate dehydrogenase E1 component OS=Mycobacterium tuberculosis GN=aceE PE=3 SV=1

+109

Accession Score Description
1 GLPD_PSETO 47 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

-110

Accession Score Description
1 EFTS_PSEPG 47 Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
Score Mass Matches Sequences emPAI
110.1 EFTS_PSEPG 47 30483 6 (2) 5 (2) 0.16
Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
4 samesets of EFTS_PSEPG
EFTS_PSEPK 47 30527 6 (2) 5 (2) 0.16
Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
EFTS_PSEPW 47 30452 6 (2) 5 (2) 0.16
Elongation factor Ts OS=Pseudomonas putida (strain W619) GN=tsf PE=3 SV=1
EFTS_PSEP1 47 30513 6 (2) 5 (2) 0.16
Elongation factor Ts OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tsf PE=3 SV=1
EFTS_PSEE4 47 30581 6 (2) 5 (2) 0.16
Elongation factor Ts OS=Pseudomonas entomophila (strain L48) GN=tsf PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
469   347.6808 693.3470 693.3333 19.8 1 13 1.4 +6Score > 42 indicates identity
Score > 27 indicates homology
U E.EAFAQK.L + Deamidated (NQ)
697   399.7778 797.5410 797.5375 4.48 0 37 0.013 +1Score > 30 indicates identity U K.IGAVVVLK.G
818   429.2781 856.5416 856.5382 4.05 0 23 0.22 +1Score > 36 indicates identity
Score > 29 indicates homology
M.AAITAALVK.E
1419 +1 564.3269 1126.6392 1126.6346 4.13 0 39 0.0034 +1Score > 39 indicates identity
Score > 27 indicates homology
U K.LTDAAPLIASR.E
2186   486.5863 1456.7371 1456.7310 4.13 1 11 0.87 +2Score > 40 indicates identity
Score > 23 indicates homology
U R.VEGDVVGAYLHGNK.I

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