MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

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-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1352 43793 90 (56) 18 (15) 3.17
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1352 43793 90 (56) 18 (15) 3.17
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1236 43810 87 (53) 18 (15) 3.39
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1236 43810 87 (53) 18 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1236 43810 87 (53) 18 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1236 43810 87 (53) 18 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_CARRP 180 44439 15 (8) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
EFTU_CYAP7 34 44885 9 (3) 3 (2) 0.10
Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1

-108 peptide matches (49 non-duplicate, 59 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
462 +1 689.3958 688.3885 688.3868 2.49 0 33 0.18 +1Score > 43 indicates identity
Score > 38 indicates homology		 X X R.GTVVTGR.I
463 +2 345.2021 688.3896 688.3868 4.12 0 35 0.1 +1Score > 41 indicates identity
Score > 38 indicates homology		 X X R.GTVVTGR.I
486 +1 702.3802 701.3729 701.3708 3.04 1 39 0.13 +1Score > 45 indicates identity
Score > 42 indicates homology		 X X K.LLDEGR.A
487 +2 351.6938 701.3730 701.3708 3.22 1 28 0.31 +1Score > 45 indicates identity
Score > 35 indicates homology		 X X K.LLDEGR.A
648 +4 388.7092 775.4038 775.4017 2.75 0 31 0.0075 +1Score > 42 indicates identity
Score > 22 indicates homology		 U X X X X R.HTPFFK  
653   776.4139 775.4066 775.4017 6.33 0 33 0.074 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X X X X R.HTPFFK  
704 +2 401.2466 800.4786 800.4756 3.79 0 42 0.0025 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X X R.TVGAGVVAK.I
707 -2 801.4884 800.4811 800.4756 6.88 0 55 0.0018 +1Score > 40 indicates identity U X X R.TVGAGVVAK.I
705   801.4860 800.4787 800.4756 3.88 0 (54) 0.0038 +1Score > 42 indicates identity U X X R.TVGAGVVAK.I
709   801.4966 800.4893 800.4756 17.1 0 (49) 0.0081 +1Score > 40 indicates identity U X X R.TVGAGVVAK.I
839   867.5076 866.5003 866.4974 3.40 1 48 0.0038 +1Score > 37 indicates identity U X X R.EHILLSR.Q
840 +2 434.2578 866.5010 866.4974 4.24 1 40 0.022 +1Score > 36 indicates identity U X X R.EHILLSR.Q
1028 +2 947.5555 946.5482 946.5447 3.68 0 62 0.00016 +1Score > 38 indicates identity
Score > 37 indicates homology		 U X X K.TTLTAALTR.V
1029 +3 474.2814 946.5482 946.5447 3.71 0 81 9.1e-007 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X K.TTLTAALTR.V
1169 +1 503.2572 1004.4998 1004.4961 3.77 1 41 0.013 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X X K.TIAMEDGLR.F
1345   544.7821 1087.5496 1087.5444 4.81 1 73 1.3e-005 +1Score > 42 indicates identity
Score > 36 indicates homology		 U X X R.AGENCGVLLR.G
1489   1156.6339 1155.6266 1155.6176 7.84 1 49 0.006 +1Score > 39 indicates identity U X X K.FTAEVYVLSK.E
1491 +4 578.8210 1155.6274 1155.6176 8.55 1 47 0.0013 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X X K.FTAEVYVLSK.E
1699 +2 411.8787 1232.6143 1232.6091 4.22 0 31 0.022 +1Score > 42 indicates identity
Score > 27 indicates homology		 U X X K.GYRPQFYFR.T
1701 +1 617.3152 1232.6158 1232.6091 5.50 0 20 0.74 +2Score > 42 indicates identity
Score > 31 indicates homology		 U X X K.GYRPQFYFR.T
1711   413.5787 1237.7143 1237.7030 9.08 1 42 0.013 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
1713 +4 619.8661 1237.7176 1237.7030 11.8 1 69 2.5e-005 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
2224 +1 492.6310 1474.8712 1474.8548 11.1 0 62 5e-005 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2226 +2 738.4444 1474.8742 1474.8548 13.2 0 79 9.6e-007 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2448 +2 315.7941 1573.9341 1573.9304 2.35 0 12 1 +1Score > 30 indicates identity
Score > 24 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2453 +2 525.6529 1573.9369 1573.9304 4.10 0 44 0.0012 +1Score > 29 indicates identity
Score > 27 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2455 +1 394.4915 1573.9369 1573.9304 4.11 0 21 0.019 +1Score > 29 indicates identity
Score > 16 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2456   788.4741 1574.9336 1574.9144 12.2 0 42 0.0039 +1Score > 30 indicates identity U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2457   394.7425 1574.9409 1574.9144 16.8 0 5 0.76 +1Score > 29 indicates identity
Score > 16 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2555 +3 807.9489 1613.8832 1613.8665 10.4 1 78 1.1e-006 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X K.LVETLDAYIPEPVR.A
2558   538.9713 1613.8921 1613.8665 15.9 1 20 0.11 +1Score > 38 indicates identity
Score > 23 indicates homology		 U X K.LVETLDAYIPEPVR.A
2582 +1 815.9465 1629.8784 1629.8614 10.5 1 87 4.5e-007 +1Score > 39 indicates identity
Score > 36 indicates homology		 U X K.LVETLDSYIPEPVR.A
2583   544.3013 1629.8821 1629.8614 12.7 1 15 0.71 +1Score > 39 indicates identity
Score > 26 indicates homology		 U X K.LVETLDSYIPEPVR.A
2841   883.9711 1765.9276 1765.9224 2.98 0 82 2.3e-006 +1Score > 39 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2842 +1 354.1930 1765.9286 1765.9224 3.53 0 29 0.51 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2844   354.3934 1766.9306 1766.9064 13.7 0 22 0.16 +1Score > 38 indicates identity
Score > 27 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2845 +2 442.7403 1766.9321 1766.9064 14.5 0 44 0.00037 +1Score > 38 indicates identity
Score > 22 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2849 +1 589.9859 1766.9359 1766.9064 16.7 0 46 0.00097 +1Score > 38 indicates identity
Score > 29 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2850   442.9534 1767.7845 1767.7787 3.25 0 15 0.065 +1Score > 39 indicates identity
Score > 15 indicates homology		 X X X R.HYAHVDCPGHADYVK.N
2879   596.9826 1787.9260 1787.9166 5.24 1 44 0.0016 +1Score > 40 indicates identity
Score > 28 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2880   894.9749 1787.9352 1787.9166 10.4 1 59 2.8e-005 +1Score > 40 indicates identity
Score > 26 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2884   597.3157 1788.9253 1788.9006 13.8 1 39 0.0016 +1Score > 40 indicates identity
Score > 24 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2885   895.4705 1788.9264 1788.9006 14.4 1 80 1.6e-006 +1Score > 40 indicates identity
Score > 35 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2901 +1 601.3138 1800.9196 1800.9118 4.30 1 68 9.2e-005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2912   601.6479 1801.9219 1801.8958 14.4 1 50 0.005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2913 +1 901.9699 1801.9252 1801.8958 16.3 1 93 2.9e-007 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
3419   1068.5586 2135.1026 2135.0609 19.6 1 93 1.1e-007 +1Score > 39 indicates identity
Score > 35 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3449   1076.5562 2151.0978 2151.0558 19.5 1 67 6.7e-007 +1Score > 39 indicates identity
Score > 17 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ); Oxidation (M)
3453 +1 718.3775 2152.1107 2152.0874 10.8 1 25 0.0074 +1Score > 38 indicates identity
Score > 16 indicates homology		 U X R.DIDKPFLMAIEDVFSISGR.G
3530 +2 1097.0687 2192.1228 2192.1551 -14.7 1 48 0.00016 +1Score > 39 indicates identity
Score > 22 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3533 +5 731.7173 2192.1301 2192.1551 -11.4 1 51 4.7e-005 +1Score > 38 indicates identity
Score > 20 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)

+39 subsets and intersections (412 subset proteins in total)

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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