MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
131 489.2804 488.2731 488.2707 4.95 0 15 0.39 +1Score > 44 indicates identity
Score > 24 indicates homology		 TVGGR
1366 381.8934 1142.6584 1142.6771 -16.4 1 15 0.63 +1Score > 39 indicates identity
Score > 26 indicates homology		 ALKSTIGVAGAR
1689 644.8590 1287.7034 1287.7187 -11.8 1 15 0.78 +1Score > 40 indicates identity
Score > 27 indicates homology		 ALGFILAQGLRE + Deamidated (NQ)
2313 791.8985 1581.7824 1581.7998 -11.0 1 15 0.67 +1Score > 41 indicates identity
Score > 26 indicates homology		 HVDDILESLQGLSR + Deamidated (NQ)
1780 665.3510 1328.6874 1328.6936 -4.62 1 15 0.46 +1Score > 41 indicates identity
Score > 24 indicates homology		 QVVVNLLGSRGGE + 2 Deamidated (NQ)
2215 772.4291 1542.8436 1542.8439 -0.18 0 15 0.98 +1Score > 38 indicates identity
Score > 27 indicates homology		 NMVVSNILLNNIAK + Deamidated (NQ)
489 375.1997 748.3848 748.3728 16.0 0 15 0.73 +1Score > 42 indicates identity
Score > 26 indicates homology		 AASHHAR
1768 663.3423 1324.6700 1324.6551 11.3 1 15 0.71 +1Score > 40 indicates identity
Score > 26 indicates homology		 VYPVVGTVYAEE
1038 494.2486 986.4826 986.4920 -9.50 0 15 0.58 +1Score > 40 indicates identity
Score > 25 indicates homology		 QQLQQLVE + 2 Deamidated (NQ)
3090 671.3553 2011.0441 2011.0262 8.89 1 15 0.47 +1Score > 39 indicates identity
Score > 24 indicates homology		 QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
1157 518.2985 1034.5824 1034.6012 -18.1 1 15 0.39 +1Score > 38 indicates identity
Score > 23 indicates homology		 IEIYIVGTK
2491 556.6271 1666.8595 1666.8777 -11.0 1 15 0.097 +1Score > 39 indicates identity
Score > 17 indicates homology		 ITATSVPPIINIEGNE
2744 900.9780 1799.9414 1799.9418 -0.17 0 15 0.91 +1Score > 39 indicates identity
Score > 27 indicates homology		 ITTVQAAIDFIHTNQK + Deamidated (NQ)
1155 518.2807 1034.5468 1034.5396 6.98 1 15 0.3 +1Score > 41 indicates identity
Score > 22 indicates homology		 AFSRQLAIE + Deamidated (NQ)
1713 649.8433 1297.6720 1297.6738 -1.37 1 15 0.62 +1Score > 40 indicates identity
Score > 25 indicates homology		 NVTPNNNIRQK + Deamidated (NQ)
2316 792.9399 1583.8652 1583.8519 8.45 1 15 0.74 +1Score > 38 indicates identity
Score > 26 indicates homology		 NEIQVLNVVTADLR + Deamidated (NQ)
2778 906.9928 1811.9710 1811.9352 19.8 1 15 0.15 +1Score > 38 indicates identity
Score > 19 indicates homology		 EPMIQVAKNFTGLAHR + Deamidated (NQ)
471 368.2252 734.4358 734.4326 4.36 0 15 0.66 +1Score > 36 indicates identity
Score > 26 indicates homology		 IALYGAK
3168 687.0252 2058.0538 2058.0278 12.6 1 15 0.42 +1Score > 39 indicates identity
Score > 24 indicates homology		 VMFNTAFIRSNILMLNR + 3 Deamidated (NQ); Oxidation (M)
2172 759.9386 1517.8626 1517.8705 -5.15 1 15 0.76 +1Score > 35 indicates identity
Score > 26 indicates homology		 ISLVNIKYLVLDE
354 686.3288 685.3215 685.3184 4.62 0 15 0.89 +1Score > 37 indicates identity
Score > 27 indicates homology		 YGYGAR
1709 649.3489 1296.6832 1296.6898 -5.07 1 15 0.12 +1Score > 40 indicates identity
Score > 18 indicates homology		 NVTPNNNIRQK
2054 734.4393 1466.8640 1466.8933 -19.9 1 15 0.71 +1Score > 32 indicates identity
Score > 26 indicates homology		 GIVRADLVLIGVSR
3449 732.3865 2194.1377 2194.1239 6.30 1 15 0.081 +1Score > 38 indicates identity
Score > 16 indicates homology		 GNLGMQLGFADLMGVLSKGVR + 2 Oxidation (M)
2088 743.4306 1484.8466 1484.8311 10.5 1 15 0.64 +1Score > 36 indicates identity
Score > 25 indicates homology		 IQQVLNTASKLNR + Deamidated (NQ)
1735 656.3736 1310.7326 1310.7558 -17.7 1 15 0.93 +1Score > 38 indicates identity
Score > 27 indicates homology		 IIGVEPDRVSVK
2165 758.8740 1515.7334 1515.7529 -12.8 1 15 0.5 +1Score > 41 indicates identity
Score > 24 indicates homology		 SQAQQQLIDSLRE + Deamidated (NQ)
1566 410.5766 1228.7080 1228.7139 -4.86 1 15 0.92 +1Score > 38 indicates identity
Score > 27 indicates homology		 QTQVTVQKIGK
3000 978.5424 1955.0702 1955.0663 2.04 0 15 0.19 +1Score > 36 indicates identity
Score > 20 indicates homology		 NLMSTHSLGIVFGPTLLR
679 421.7233 841.4320 841.4294 3.20 0 15 0.98 +1Score > 39 indicates identity
Score > 27 indicates homology		 NPDNLIR + Deamidated (NQ)
967 479.2561 956.4976 956.4927 5.18 0 15 0.93 +1Score > 40 indicates identity
Score > 27 indicates homology		 TITAHAQSK + Deamidated (NQ)
3483 1105.1327 2208.2508 2208.2531 -1.02 1 15 0.31 +1Score > 31 indicates identity
Score > 22 indicates homology		 NIINLEPVLNVLRWHLHK + Deamidated (NQ)
248 315.2031 628.3916 628.3908 1.34 0 15 0.77 +1Score > 42 indicates identity
Score > 26 indicates homology		 LGNVVK
2175 507.9507 1520.8303 1520.8133 11.2 1 15 0.51 +1Score > 38 indicates identity
Score > 24 indicates homology		 AITGFLRNQLTMR + Deamidated (NQ)
1374 574.3143 1146.6140 1146.6132 0.72 1 15 0.29 +1Score > 41 indicates identity
Score > 22 indicates homology		 ISISGKTDVVE
2190 765.4371 1528.8596 1528.8712 -7.56 1 15 0.71 +1Score > 36 indicates identity
Score > 26 indicates homology		 LQTAITLIDELTAK
499 379.7250 757.4354 757.4334 2.73 1 15 0.87 +1Score > 43 indicates identity
Score > 27 indicates homology		 AVAAVAKE
3635 769.0392 2304.0958 2304.0586 16.1 1 15 0.51 +1Score > 39 indicates identity
Score > 24 indicates homology		 NKIFQNFFPDLFSSLQNDE + 2 Deamidated (NQ)
254 630.4202 629.4129 629.4112 2.72 0 15 0.64 +1Score > 38 indicates identity
Score > 25 indicates homology		 IVTGLK
831 451.2550 900.4954 900.5029 -8.22 1 15 0.55 +1Score > 42 indicates identity
Score > 25 indicates homology		 QDLRAGLK + Deamidated (NQ)
1200 531.7677 1061.5208 1061.5254 -4.28 1 15 0.68 +1Score > 42 indicates identity
Score > 25 indicates homology		 HPGAEVAQPR + Deamidated (NQ)
1482 597.8578 1193.7010 1193.7019 -0.76 0 15 0.64 +1Score > 33 indicates identity
Score > 25 indicates homology		 QLIVNATLPPK + Deamidated (NQ)
1122 510.3031 1018.5916 1018.5811 10.3 1 15 0.92 +1Score > 37 indicates identity
Score > 27 indicates homology		 LATVEALFR
847 455.7574 909.5002 909.4994 0.98 1 15 0.85 +1Score > 38 indicates identity
Score > 26 indicates homology		 MLKDFIK + Oxidation (M)
4257 962.8588 2885.5546 2885.5036 17.7 1 15 1 +1Score > 33 indicates identity
Score > 27 indicates homology		 GWGQSVIIGVAGAGQEISTRPFQLVTGR + 2 Deamidated (NQ)
199 602.3603 601.3530 601.3435 15.8 0 15 0.82 +1Score > 45 indicates identity
Score > 26 indicates homology		 ISSAPK
443 364.2178 726.4210 726.4276 -9.02 0 15 0.35 +1Score > 36 indicates identity
Score > 23 indicates homology		 GPVLVDK
3234 1051.0098 2100.0050 2100.0236 -8.84 1 15 0.31 +1Score > 39 indicates identity
Score > 22 indicates homology		 SLANDGYRVVVSGTDQTYR
1493 600.3370 1198.6594 1198.6670 -6.27 1 15 0.98 +1Score > 40 indicates identity
Score > 27 indicates homology		 GRNAIISLQVE
1853 684.3538 1366.6930 1366.6915 1.15 0 15 0.41 +1Score > 40 indicates identity
Score > 23 indicates homology		 GTYVCIATNALGK
4273 966.1858 2895.5356 2895.5011 11.9 1 15 0.1 +1Score > 34 indicates identity
Score > 17 indicates homology		 LLVVLNDNDMSISPPVGALSNVLNRAR + 3 Deamidated (NQ); Oxidation (M)
2431 816.4193 1630.8240 1630.8171 4.27 1 15 0.54 +1Score > 41 indicates identity
Score > 24 indicates homology		 GEATPSQMGGLLMALR
2552 846.4758 1690.9370 1690.9328 2.54 1 15 0.62 +1Score > 36 indicates identity
Score > 25 indicates homology		 LIQIACGKGYLDIVK + Deamidated (NQ)
1520 606.3566 1210.6986 1210.6809 14.7 1 14 0.75 +1Score > 36 indicates identity
Score > 26 indicates homology		 VIVPEAITIQE
1555 613.3502 1224.6858 1224.6866 -0.64 1 14 0.69 +1Score > 38 indicates identity
Score > 25 indicates homology		 ISIKNISFFR + Deamidated (NQ)
1159 518.2994 1034.5842 1034.6012 -16.4 1 14 0.54 +1Score > 38 indicates identity
Score > 24 indicates homology		 IEIYIVGTK
1249 362.8777 1085.6113 1085.6081 2.96 1 14 0.53 +1Score > 40 indicates identity
Score > 24 indicates homology		 TLEQLGLAGGK
3022 657.6730 1969.9972 1969.9927 2.29 1 14 0.68 +1Score > 39 indicates identity
Score > 25 indicates homology		 ISMMFAIMGVAATKLLPE + 3 Oxidation (M)
3667 773.7302 2318.1688 2318.1423 11.4 1 14 0.7 +1Score > 38 indicates identity
Score > 25 indicates homology		 TATSMQDIVNIIDAARLDANGK + 2 Deamidated (NQ)
3520 1117.6500 2233.2854 2233.2583 12.2 1 14 0.75 +1Score > 29 indicates identity
Score > 26 indicates homology		 SLTRFGFTLIIGGAIGNLIDR
1605 620.9055 1239.7964 1239.7802 13.1 1 14 0.46 +1Score > 24 indicates identity IIGISAIAKLIE
4176 933.4874 2797.4404 2797.4684 -10.0 1 14 0.088 +1Score > 36 indicates identity
Score > 16 indicates homology		 SPEPAHPIALDALGLTPLVDLGMRLGE + Oxidation (M)
2589 570.9699 1709.8879 1709.8584 17.2 0 14 0.58 +1Score > 39 indicates identity
Score > 25 indicates homology		 IVNNSDNPLQAGQIVE
3609 763.4367 2287.2883 2287.2827 2.42 0 14 0.2 +1Score > 31 indicates identity
Score > 20 indicates homology		 VLFLQGGASLQFSAIPLNLLGK + 2 Deamidated (NQ)
2001 359.6994 1434.7685 1434.7752 -4.66 1 14 0.76 +1Score > 39 indicates identity
Score > 26 indicates homology		 LTMLLTNQKSIR + 2 Deamidated (NQ); Oxidation (M)
2104 499.2220 1494.6442 1494.6297 9.71 1 14 0.11 +1Score > 38 indicates identity
Score > 17 indicates homology		 AEPTEQSFMQPGR + 2 Deamidated (NQ); Oxidation (M)
179 586.3876 585.3803 585.3737 11.2 0 14 0.37 +1Score > 39 indicates identity
Score > 23 indicates homology		 ILDIL
3524 746.4068 2236.1986 2236.2150 -7.36 1 14 0.24 +1Score > 36 indicates identity
Score > 21 indicates homology		 MFLQARAALVTGGALYIVGNR + Oxidation (M)
216 306.7083 611.4020 611.4119 -16.1 0 14 0.57 +1Score > 32 indicates identity
Score > 24 indicates homology		 IVKPR
2803 609.9794 1826.9164 1826.9526 -19.8 0 14 0.23 +1Score > 40 indicates identity
Score > 20 indicates homology		 IPTLSQVQALSADWAAR + Deamidated (NQ)
2996 651.3317 1950.9733 1950.9575 8.11 1 14 0.46 +1Score > 40 indicates identity
Score > 24 indicates homology		 VLADIQQGRFALDFVQE + 3 Deamidated (NQ)
2333 531.6163 1591.8271 1591.8392 -7.60 1 14 0.97 +1Score > 40 indicates identity
Score > 27 indicates homology		 AALKLACDLYALDR
798 446.2429 890.4712 890.4644 7.74 1 14 0.94 +1Score > 41 indicates identity
Score > 27 indicates homology		 MLAARGQK + Deamidated (NQ); Oxidation (M)
1445 589.2944 1176.5742 1176.5523 18.6 0 14 0.49 +1Score > 42 indicates identity
Score > 24 indicates homology		 AQNAFGALNDR + Deamidated (NQ)
2878 939.4792 1876.9438 1876.9782 -18.3 1 14 0.97 +1Score > 40 indicates identity
Score > 27 indicates homology		 YLNAVGSVLDLSIEDLR + Deamidated (NQ)
210 302.6880 603.3614 603.3704 -14.8 1 14 0.94 +1Score > 40 indicates identity
Score > 27 indicates homology		 ISKTR
1460 591.8790 1181.7434 1181.7635 -16.9 1 14 0.91 +1Score > 30 indicates identity
Score > 26 indicates homology		 LVQLIIELIK + Deamidated (NQ)
2117 749.9406 1497.8666 1497.8402 17.6 1 14 0.42 +1Score > 35 indicates identity
Score > 23 indicates homology		 VVNQLLSKAGNLLE + Deamidated (NQ)
2702 886.9547 1771.8948 1771.8815 7.56 1 14 0.14 +1Score > 40 indicates identity
Score > 18 indicates homology		 VNFSVNFKCLSSQIK + 2 Deamidated (NQ)
1189 353.5279 1057.5619 1057.5768 -14.1 1 14 0.2 +1Score > 42 indicates identity
Score > 20 indicates homology		 KAQQVLGVSE
1363 571.8627 1141.7108 1141.7070 3.33 0 14 0.58 +1Score > 30 indicates identity
Score > 24 indicates homology		 ISIQVLQTLK
1856 685.3399 1368.6652 1368.6925 -19.9 0 14 0.88 +1Score > 41 indicates identity
Score > 26 indicates homology		 ALNSVYSNYLPK + Deamidated (NQ)
2003 718.9134 1435.8122 1435.7922 14.0 1 14 0.56 +1Score > 35 indicates identity
Score > 24 indicates homology		 AISKILDAISDYK
2633 866.8903 1731.7660 1731.7807 -8.49 0 14 0.25 +1Score > 39 indicates identity
Score > 21 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
4087 903.4874 2707.4404 2707.4268 5.01 1 14 0.11 +1Score > 35 indicates identity
Score > 17 indicates homology		 WIGSVNSIGMAAGAFLFGLLADRIGR + Oxidation (M)
2119 500.5883 1498.7431 1498.7627 -13.1 1 14 0.94 +1Score > 40 indicates identity
Score > 26 indicates homology		 VANGQVVAELIDDR + Deamidated (NQ)
2167 758.9329 1515.8512 1515.8330 12.0 1 14 0.48 +1Score > 36 indicates identity
Score > 24 indicates homology		 VNAAALIEMVLTGAK + Oxidation (M)
3260 706.3517 2116.0333 2116.0293 1.89 1 14 0.095 +1Score > 39 indicates identity
Score > 17 indicates homology		 MTVLSSSLPNVGHGALTMRE + Deamidated (NQ); Oxidation (M)
765 438.2291 874.4436 874.4548 -12.8 1 14 0.64 +1Score > 44 indicates identity
Score > 25 indicates homology		 QATELWK
2121 750.4443 1498.8740 1498.8541 13.3 1 14 0.79 +1Score > 33 indicates identity
Score > 26 indicates homology		 NKLLIIDGMNLVR + Deamidated (NQ)
3888 835.0896 2502.2470 2502.2446 0.94 1 14 0.17 +1Score > 38 indicates identity
Score > 19 indicates homology		 TAQMGWSLARLIAQMGWGAPAMR
164 559.3602 558.3529 558.3489 7.14 0 14 0.83 +1Score > 40 indicates identity
Score > 26 indicates homology		 ITGIR
1801 671.3862 1340.7578 1340.7551 2.04 0 14 0.3 +1Score > 37 indicates identity
Score > 22 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
2314 528.2688 1581.7846 1581.7998 -9.65 1 14 0.7 +1Score > 41 indicates identity
Score > 25 indicates homology		 HVDDILESLQGLSR + Deamidated (NQ)
2780 907.9416 1813.8686 1813.8992 -16.9 1 14 0.63 +1Score > 40 indicates identity
Score > 25 indicates homology		 SHEPKSPSLGMISTATR + Oxidation (M)
1436 586.8557 1171.6968 1171.6812 13.3 1 14 0.52 +1Score > 35 indicates identity
Score > 24 indicates homology		 LTLKLQTLNE
1800 671.3820 1340.7494 1340.7551 -4.22 0 14 0.87 +1Score > 38 indicates identity
Score > 26 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
699 426.2123 850.4100 850.4072 3.32 1 14 0.63 +1Score > 42 indicates identity
Score > 25 indicates homology		 QLEFADK + Deamidated (NQ)
1444 588.8204 1175.6262 1175.6411 -12.6 1 14 0.78 +1Score > 41 indicates identity
Score > 26 indicates homology		 RYVVAADGVAR
965 319.8394 956.4964 956.4927 3.83 1 14 0.72 +1Score > 40 indicates identity
Score > 25 indicates homology		 TPVAEDVAR
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