MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2335 532.2677 1593.7813 1593.7787 1.60 0 16 0.87 +1Score > 41 indicates identity
Score > 28 indicates homology
DAYAVTPFNTPTAAR
2858 934.0628 1866.1110 1866.1078 1.76 0 16 0.19 +1Score > 27 indicates identity
Score > 21 indicates homology
IGIVQGVILSQQIVITAL + 2 Deamidated (NQ)
491 375.7244 749.4342 749.4323 2.56 1 16 0.86 +1Score > 36 indicates identity
Score > 28 indicates homology
KFLTIE
3070 667.0054 1997.9944 1997.9656 14.4 0 16 0.18 +1Score > 40 indicates identity
Score > 21 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
1340 567.8161 1133.6176 1133.5975 17.8 1 16 0.86 +1Score > 40 indicates identity
Score > 28 indicates homology
VSGASITMRGR
2930 637.0154 1908.0244 1908.0325 -4.26 1 16 0.24 +1Score > 37 indicates identity
Score > 22 indicates homology
MRSLSSLVVPMVSIAFR + Oxidation (M)
554 390.2201 778.4256 778.4225 4.06 0 16 0.33 +1Score > 40 indicates identity
Score > 24 indicates homology
AVISNFK + Deamidated (NQ)
425 358.2460 714.4774 714.4639 18.9 0 16 0.75 +1Score > 36 indicates identity
Score > 27 indicates homology
AALILSK
191 300.7088 599.4030 599.4006 4.03 0 16 0.91 +1Score > 40 indicates identity
Score > 28 indicates homology
IAGVLK
952 316.8563 947.5471 947.5328 15.1 1 16 0.38 +1Score > 38 indicates identity
Score > 24 indicates homology
YSLPEVIK
1202 532.2775 1062.5404 1062.5267 13.0 1 16 0.99 +1Score > 42 indicates identity
Score > 29 indicates homology
ALDLMDLEK + Oxidation (M)
636 410.7259 819.4372 819.4524 -18.5 1 16 0.98 +1Score > 41 indicates identity
Score > 28 indicates homology
KMSDVIK
1914 699.8871 1397.7596 1397.7514 5.90 1 16 0.99 +1Score > 39 indicates identity
Score > 28 indicates homology
VLALLNDNLQRE + Deamidated (NQ)
3978 862.8154 2585.4244 2585.3840 15.6 1 16 1 +1Score > 32 indicates identity
Score > 29 indicates homology
SKGSATALYSQLLDTTLQILYLGK + 2 Deamidated (NQ)
177 584.3431 583.3358 583.3330 4.91 0 16 0.66 +1Score > 32 indicates identity
Score > 27 indicates homology
QLGVAP
1370 572.8225 1143.6304 1143.6248 4.96 1 16 0.87 +1Score > 40 indicates identity
Score > 28 indicates homology
AIQQDTNKVK
1018 325.1952 972.5638 972.5604 3.50 1 16 0.75 +1Score > 39 indicates identity
Score > 27 indicates homology
KQQLSLQK + Deamidated (NQ)
666 417.2498 832.4850 832.4807 5.24 1 16 0.44 +1Score > 38 indicates identity
Score > 25 indicates homology
IFGEVIR
2709 592.3220 1773.9442 1773.9665 -12.6 1 16 0.21 +1Score > 38 indicates identity
Score > 22 indicates homology
LNLVVQDFIFKHTAK + 2 Deamidated (NQ)
1747 660.3099 1318.6052 1318.6153 -7.65 0 16 0.43 +1Score > 40 indicates identity
Score > 25 indicates homology
TLDISDADWQR
1902 464.2612 1389.7618 1389.7504 8.22 1 16 0.67 +1Score > 39 indicates identity
Score > 27 indicates homology
GLFVAISNQEIAK + Deamidated (NQ)
3091 671.3569 2011.0489 2011.0262 11.3 1 16 0.46 +1Score > 39 indicates identity
Score > 25 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
1166 521.7799 1041.5452 1041.5342 10.6 0 16 0.83 +1Score > 39 indicates identity
Score > 28 indicates homology
VPNNDLQLK + 2 Deamidated (NQ)
1964 711.3478 1420.6810 1420.6908 -6.86 1 16 0.59 +1Score > 41 indicates identity
Score > 26 indicates homology
LQANVFEMDIPK + Deamidated (NQ); Oxidation (M)
2667 880.4536 1758.8926 1758.8682 13.9 1 16 0.61 +1Score > 40 indicates identity
Score > 26 indicates homology
QLQLAMHNSKAASSTR + Deamidated (NQ); Oxidation (M)
2334 797.4008 1592.7870 1592.7716 9.71 1 16 0.7 +1Score > 41 indicates identity
Score > 27 indicates homology
LSMKAVATDVADNAAT + Oxidation (M)
2776 906.9569 1811.8992 1811.8981 0.61 0 16 0.84 +1Score > 40 indicates identity
Score > 28 indicates homology
EPPPPPPPPPPPQIEPE + Deamidated (NQ)
3230 525.0211 2096.0553 2096.0684 -6.26 0 16 0.16 +1Score > 39 indicates identity
Score > 20 indicates homology
GDITSVAAMASLNLSQPHLR + Oxidation (M)
327 336.7128 671.4110 671.4078 4.78 1 16 0.55 +1Score > 42 indicates identity
Score > 26 indicates homology
AGAKGIR
3313 1068.5619 2135.1092 2135.0858 11.0 1 16 0.15 +1Score > 38 indicates identity
Score > 20 indicates homology
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
2096 497.9290 1490.7652 1490.7828 -11.8 0 16 0.15 +1Score > 41 indicates identity
Score > 20 indicates homology
TSALALSSLSGDIQK + Deamidated (NQ)
933 471.7761 941.5376 941.5546 -18.0 0 16 0.84 +1Score > 40 indicates identity
Score > 28 indicates homology
QGVVDLLAK
2063 736.8677 1471.7208 1471.7381 -11.7 0 16 0.69 +1Score > 40 indicates identity
Score > 27 indicates homology
WALMIDPQGQALK + 2 Deamidated (NQ)
3381 724.4366 2170.2880 2170.2911 -1.46 0 16 0.51 +1Score > 25 indicates identity LIIFAMNGVLVAIAGLILSAR + Oxidation (M)
3009 653.6844 1958.0314 1958.0275 1.98 0 16 0.27 +1Score > 38 indicates identity
Score > 23 indicates homology
LFPSTHALQPHLSAHFR
2241 519.3005 1554.8797 1554.8558 15.3 1 16 0.67 +1Score > 36 indicates identity
Score > 27 indicates homology
RFPVALALSAPVWE
1327 566.2827 1130.5508 1130.5642 -11.8 0 16 0.92 +1Score > 41 indicates identity
Score > 28 indicates homology
VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
217 307.1816 612.3486 612.3595 -17.7 0 16 0.97 +1Score > 38 indicates identity
Score > 28 indicates homology
IEPVR
1039 494.2656 986.5166 986.5033 13.5 0 16 0.73 +1Score > 42 indicates identity
Score > 27 indicates homology
GGEPGVSGTVK
1972 712.3618 1422.7090 1422.7354 -18.5 1 16 0.95 +1Score > 41 indicates identity
Score > 28 indicates homology
LQSQIQSKFQSK + 2 Deamidated (NQ)
183 587.3525 586.3452 586.3439 2.33 0 16 0.57 +1Score > 44 indicates identity
Score > 26 indicates homology
AGNVVK
1657 637.8123 1273.6100 1273.6037 4.96 1 16 0.5 +1Score > 41 indicates identity
Score > 25 indicates homology
DEINDALINQK + 2 Deamidated (NQ)
1178 349.8419 1046.5039 1046.5179 -13.4 0 16 0.76 +1Score > 42 indicates identity
Score > 27 indicates homology
MVQNGTIAGR + Deamidated (NQ)
1750 660.3894 1318.7642 1318.7497 11.1 1 16 0.68 +1Score > 34 indicates identity
Score > 27 indicates homology
FLGTTKDPVTLK
3893 836.4256 2506.2550 2506.2295 10.2 1 16 0.083 +1Score > 38 indicates identity
Score > 17 indicates homology
VNTMLLSTSVSRLMQPADTPSLE + Deamidated (NQ); Oxidation (M)
507 380.7233 759.4320 759.4279 5.44 0 16 0.81 +1Score > 40 indicates identity
Score > 27 indicates homology
LFDPLR
2403 808.4290 1614.8434 1614.8729 -18.2 1 16 0.4 +1Score > 40 indicates identity
Score > 24 indicates homology
RNFLNQAASLLLPR + 3 Deamidated (NQ)
3160 685.3321 2052.9745 2052.9851 -5.19 0 16 0.34 +1Score > 39 indicates identity
Score > 24 indicates homology
TNAVSLTNVYDQVSQGNIK + 3 Deamidated (NQ)
1115 508.7704 1015.5262 1015.5338 -7.45 1 16 0.72 +1Score > 42 indicates identity
Score > 27 indicates homology
LPLEPEYR
2109 748.8986 1495.7826 1495.8021 -13.0 1 16 0.88 +1Score > 39 indicates identity
Score > 28 indicates homology
IPIELLQVNDIVE + 2 Deamidated (NQ)
2394 807.9485 1613.8824 1613.8988 -10.2 1 16 0.81 +1Score > 38 indicates identity
Score > 27 indicates homology
TGTVTPNVTKAVADIK
3567 754.0705 2259.1897 2259.1575 14.2 1 16 0.11 +1Score > 37 indicates identity
Score > 18 indicates homology
NLLSFLRGTFLNNYVPFDK + 2 Deamidated (NQ)
647 413.7434 825.4722 825.4821 -11.9 1 16 0.97 +1Score > 37 indicates identity
Score > 28 indicates homology
IPGIDRR
1294 559.3245 1116.6344 1116.6251 8.35 1 16 0.24 +1Score > 39 indicates identity
Score > 22 indicates homology
TGTVNVTIRR + Deamidated (NQ)
2543 562.2984 1683.8734 1683.8501 13.8 1 16 0.49 +1Score > 39 indicates identity
Score > 25 indicates homology
LSPAQISELMHLSDK + Oxidation (M)
3251 705.3867 2113.1383 2113.1380 0.11 0 16 0.15 +1Score > 36 indicates identity
Score > 20 indicates homology
WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ)
750 434.2941 866.5736 866.5701 4.04 0 16 0.094 +1Score > 28 indicates identity
Score > 18 indicates homology
LGLKPLAR
2363 804.8949 1607.7752 1607.7647 6.55 0 16 0.46 +1Score > 41 indicates identity
Score > 25 indicates homology
LGADVMNMSVGNSSVK
881 307.8565 920.5477 920.5331 15.8 0 16 0.28 +1Score > 35 indicates identity
Score > 23 indicates homology
QLYLSAVK
1112 339.4994 1015.4764 1015.4822 -5.72 1 16 0.51 +1Score > 40 indicates identity
Score > 25 indicates homology
QPLNTGEQK + 2 Deamidated (NQ)
3171 1030.5322 2059.0498 2059.0884 -18.7 1 16 0.88 +1Score > 39 indicates identity
Score > 28 indicates homology
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
2487 833.4913 1664.9680 1664.9937 -15.4 1 16 0.62 +1Score > 32 indicates identity
Score > 26 indicates homology
LGIPLLKALQTQASGR
2243 519.3012 1554.8818 1554.8558 16.7 1 16 0.76 +1Score > 36 indicates identity
Score > 27 indicates homology
RFPVALALSAPVWE
180 586.3884 585.3811 585.3737 12.6 0 16 0.54 +1Score > 39 indicates identity
Score > 25 indicates homology
ILDIL
2383 806.9442 1611.8738 1611.8508 14.3 1 16 0.52 +1Score > 38 indicates identity
Score > 25 indicates homology
YAQGIQKLASQYLK + 2 Deamidated (NQ)
690 423.7283 845.4420 845.4317 12.3 1 16 0.98 +1Score > 42 indicates identity
Score > 28 indicates homology
IPDDKMK
2324 397.7162 1586.8357 1586.8264 5.87 1 16 0.53 +1Score > 40 indicates identity
Score > 25 indicates homology
VDDTIQTLQQRLR + 2 Deamidated (NQ)
2941 639.0002 1913.9788 1913.9496 15.2 1 16 0.15 +1Score > 39 indicates identity
Score > 20 indicates homology
YDLAIHAYNKNNSHVR
516 382.7206 763.4266 763.4228 5.03 1 16 0.5 +1Score > 41 indicates identity
Score > 25 indicates homology
IIAEYR
2763 904.4604 1806.9062 1806.8748 17.4 1 16 0.89 +1Score > 40 indicates identity
Score > 28 indicates homology
GLQGEQGAPGLPGSQGPAGK + 2 Deamidated (NQ)
1473 396.8768 1187.6086 1187.5975 9.31 1 16 0.53 +1Score > 42 indicates identity
Score > 25 indicates homology
KGSFPSFGFSK
854 456.7625 911.5104 911.5116 -1.31 1 15 0.96 +1Score > 38 indicates identity
Score > 28 indicates homology
KIVDYFK
517 382.7331 763.4516 763.4480 4.82 0 15 0.41 +1Score > 35 indicates identity
Score > 24 indicates homology
IIDYIK
1282 554.2530 1106.4914 1106.4914 0.076 1 15 0.54 +1Score > 40 indicates identity
Score > 25 indicates homology
VLDQMNENK + Deamidated (NQ); Oxidation (M)
1638 629.8594 1257.7042 1257.6929 9.05 1 15 0.92 +1Score > 39 indicates identity
Score > 28 indicates homology
VENVSTVNVLGK
3291 710.0477 2127.1213 2127.0960 11.9 1 15 0.6 +1Score > 38 indicates identity
Score > 26 indicates homology
AADTAGDLAGLEIAGPGFINVR
1939 704.3743 1406.7340 1406.7075 18.9 0 15 0.31 +1Score > 40 indicates identity
Score > 23 indicates homology
MLSSQVLTLDQR + Deamidated (NQ); Oxidation (M)
783 442.2543 882.4940 882.4923 1.99 1 15 0.8 +1Score > 37 indicates identity
Score > 27 indicates homology
QIPIEQR
2014 722.4155 1442.8164 1442.8093 4.98 0 15 0.25 +1Score > 37 indicates identity
Score > 22 indicates homology
IAGAGAASVLSALTNK
605 400.7729 799.5312 799.5280 4.08 1 15 0.81 +1Score > 36 indicates identity
Score > 27 indicates homology
TVRVVVK
324 336.7064 671.3982 671.4078 -14.3 1 15 0.8 +1Score > 43 indicates identity
Score > 27 indicates homology
ALQRGK
268 321.2036 640.3926 640.3908 2.87 0 15 0.88 +1Score > 35 indicates identity
Score > 27 indicates homology
AVGPLGK
592 398.1992 794.3838 794.3810 3.57 1 15 0.49 +1Score > 41 indicates identity
Score > 25 indicates homology
AVFENSK + Deamidated (NQ)
2768 904.9625 1807.9104 1807.9203 -5.47 0 15 0.42 +1Score > 40 indicates identity
Score > 24 indicates homology
AQVDALLNSPNVDIPLE + Deamidated (NQ)
1678 427.5948 1279.7626 1279.7387 18.6 0 15 0.61 +1Score > 33 indicates identity
Score > 26 indicates homology
AVSILNDLPPLK + Deamidated (NQ)
623 407.2284 812.4422 812.4392 3.76 1 15 0.85 +1Score > 39 indicates identity
Score > 27 indicates homology
AKAPPSDK
3472 735.3920 2203.1542 2203.1888 -15.7 1 15 0.29 +1Score > 37 indicates identity
Score > 23 indicates homology
FSYAVQIPLNLELLVNNQK + Deamidated (NQ)
917 469.7328 937.4510 937.4505 0.57 0 15 0.47 +1Score > 41 indicates identity
Score > 25 indicates homology
VLTGDYDR
2822 922.4930 1842.9714 1842.9574 7.60 0 15 0.34 +1Score > 39 indicates identity
Score > 23 indicates homology
TANGATIDQIAQLIDGLK + 2 Deamidated (NQ)
1648 422.9206 1265.7400 1265.7417 -1.38 0 15 0.24 +1Score > 34 indicates identity
Score > 22 indicates homology
DLPLIGMPIAVK
3089 1006.5280 2011.0414 2011.0473 -2.93 1 15 0.67 +1Score > 39 indicates identity
Score > 26 indicates homology
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
3818 812.4244 2434.2514 2434.2315 8.18 1 15 0.11 +1Score > 38 indicates identity
Score > 18 indicates homology
LLTHLDNLPQWPDQVKTMQR + 2 Deamidated (NQ)
452 365.2194 728.4242 728.4221 2.94 0 15 0.77 +1Score > 42 indicates identity
Score > 27 indicates homology
SPVWLK
1569 615.8409 1229.6672 1229.6438 19.1 1 15 0.99 +1Score > 41 indicates identity
Score > 28 indicates homology
VLNAMATIPRE + Oxidation (M)
2323 794.4061 1586.7976 1586.8093 -7.34 1 15 0.53 +1Score > 40 indicates identity
Score > 25 indicates homology
LKGFWGQDVAPIQE
1333 378.1982 1131.5728 1131.5785 -5.05 1 15 0.35 +1Score > 42 indicates identity
Score > 23 indicates homology
RSPNDIQFR
2482 554.2911 1659.8515 1659.8216 18.0 1 15 0.48 +1Score > 41 indicates identity
Score > 25 indicates homology
TYDPDTLAPALDGRR
1210 356.8546 1067.5420 1067.5499 -7.41 0 15 0.96 +1Score > 40 indicates identity
Score > 28 indicates homology
QHIDTLVLE + Deamidated (NQ)
1397 579.3073 1156.6000 1156.6088 -7.54 1 15 0.88 +1Score > 40 indicates identity
Score > 27 indicates homology
QQIRQILQE + 2 Deamidated (NQ)
921 313.8355 938.4847 938.4821 2.69 1 15 0.79 +1Score > 39 indicates identity
Score > 27 indicates homology
VPDPGEGLR
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