| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 532.2677 | 1593.7813 | 1593.7787 | 1.60 | 0 | 16 | 0.87 | 1Score > 41 indicates identityScore > 28 indicates homology |
DAYAVTPFNTPTAAR | |
| 934.0628 | 1866.1110 | 1866.1078 | 1.76 | 0 | 16 | 0.19 | 1Score > 27 indicates identityScore > 21 indicates homology |
IGIVQGVILSQQIVITAL + 2 Deamidated (NQ) | |
| 375.7244 | 749.4342 | 749.4323 | 2.56 | 1 | 16 | 0.86 | 1Score > 36 indicates identityScore > 28 indicates homology |
KFLTIE | |
| 667.0054 | 1997.9944 | 1997.9656 | 14.4 | 0 | 16 | 0.18 | 1Score > 40 indicates identityScore > 21 indicates homology |
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M) | |
| 567.8161 | 1133.6176 | 1133.5975 | 17.8 | 1 | 16 | 0.86 | 1Score > 40 indicates identityScore > 28 indicates homology |
VSGASITMRGR | |
| 637.0154 | 1908.0244 | 1908.0325 | -4.26 | 1 | 16 | 0.24 | 1Score > 37 indicates identityScore > 22 indicates homology |
MRSLSSLVVPMVSIAFR + Oxidation (M) | |
| 390.2201 | 778.4256 | 778.4225 | 4.06 | 0 | 16 | 0.33 | 1Score > 40 indicates identityScore > 24 indicates homology |
AVISNFK + Deamidated (NQ) | |
| 358.2460 | 714.4774 | 714.4639 | 18.9 | 0 | 16 | 0.75 | 1Score > 36 indicates identityScore > 27 indicates homology |
AALILSK | |
| 300.7088 | 599.4030 | 599.4006 | 4.03 | 0 | 16 | 0.91 | 1Score > 40 indicates identityScore > 28 indicates homology |
IAGVLK | |
| 316.8563 | 947.5471 | 947.5328 | 15.1 | 1 | 16 | 0.38 | 1Score > 38 indicates identityScore > 24 indicates homology |
YSLPEVIK | |
| 532.2775 | 1062.5404 | 1062.5267 | 13.0 | 1 | 16 | 0.99 | 1Score > 42 indicates identityScore > 29 indicates homology |
ALDLMDLEK + Oxidation (M) | |
| 410.7259 | 819.4372 | 819.4524 | -18.5 | 1 | 16 | 0.98 | 1Score > 41 indicates identityScore > 28 indicates homology |
KMSDVIK | |
| 699.8871 | 1397.7596 | 1397.7514 | 5.90 | 1 | 16 | 0.99 | 1Score > 39 indicates identityScore > 28 indicates homology |
VLALLNDNLQRE + Deamidated (NQ) | |
| 862.8154 | 2585.4244 | 2585.3840 | 15.6 | 1 | 16 | 1 | 1Score > 32 indicates identityScore > 29 indicates homology |
SKGSATALYSQLLDTTLQILYLGK + 2 Deamidated (NQ) | |
| 584.3431 | 583.3358 | 583.3330 | 4.91 | 0 | 16 | 0.66 | 1Score > 32 indicates identityScore > 27 indicates homology |
QLGVAP | |
| 572.8225 | 1143.6304 | 1143.6248 | 4.96 | 1 | 16 | 0.87 | 1Score > 40 indicates identityScore > 28 indicates homology |
AIQQDTNKVK | |
| 325.1952 | 972.5638 | 972.5604 | 3.50 | 1 | 16 | 0.75 | 1Score > 39 indicates identityScore > 27 indicates homology |
KQQLSLQK + Deamidated (NQ) | |
| 417.2498 | 832.4850 | 832.4807 | 5.24 | 1 | 16 | 0.44 | 1Score > 38 indicates identityScore > 25 indicates homology |
IFGEVIR | |
| 592.3220 | 1773.9442 | 1773.9665 | -12.6 | 1 | 16 | 0.21 | 1Score > 38 indicates identityScore > 22 indicates homology |
LNLVVQDFIFKHTAK + 2 Deamidated (NQ) | |
| 660.3099 | 1318.6052 | 1318.6153 | -7.65 | 0 | 16 | 0.43 | 1Score > 40 indicates identityScore > 25 indicates homology |
TLDISDADWQR | |
| 464.2612 | 1389.7618 | 1389.7504 | 8.22 | 1 | 16 | 0.67 | 1Score > 39 indicates identityScore > 27 indicates homology |
GLFVAISNQEIAK + Deamidated (NQ) | |
| 671.3569 | 2011.0489 | 2011.0262 | 11.3 | 1 | 16 | 0.46 | 1Score > 39 indicates identityScore > 25 indicates homology |
QTLSPSVLIEHLNLFGNE + Deamidated (NQ) | |
| 521.7799 | 1041.5452 | 1041.5342 | 10.6 | 0 | 16 | 0.83 | 1Score > 39 indicates identityScore > 28 indicates homology |
VPNNDLQLK + 2 Deamidated (NQ) | |
| 711.3478 | 1420.6810 | 1420.6908 | -6.86 | 1 | 16 | 0.59 | 1Score > 41 indicates identityScore > 26 indicates homology |
LQANVFEMDIPK + Deamidated (NQ); Oxidation (M) | |
| 880.4536 | 1758.8926 | 1758.8682 | 13.9 | 1 | 16 | 0.61 | 1Score > 40 indicates identityScore > 26 indicates homology |
QLQLAMHNSKAASSTR + Deamidated (NQ); Oxidation (M) | |
| 797.4008 | 1592.7870 | 1592.7716 | 9.71 | 1 | 16 | 0.7 | 1Score > 41 indicates identityScore > 27 indicates homology |
LSMKAVATDVADNAAT + Oxidation (M) | |
| 906.9569 | 1811.8992 | 1811.8981 | 0.61 | 0 | 16 | 0.84 | 1Score > 40 indicates identityScore > 28 indicates homology |
EPPPPPPPPPPPQIEPE + Deamidated (NQ) | |
| 525.0211 | 2096.0553 | 2096.0684 | -6.26 | 0 | 16 | 0.16 | 1Score > 39 indicates identityScore > 20 indicates homology |
GDITSVAAMASLNLSQPHLR + Oxidation (M) | |
| 336.7128 | 671.4110 | 671.4078 | 4.78 | 1 | 16 | 0.55 | 1Score > 42 indicates identityScore > 26 indicates homology |
AGAKGIR | |
| 1068.5619 | 2135.1092 | 2135.0858 | 11.0 | 1 | 16 | 0.15 | 1Score > 38 indicates identityScore > 20 indicates homology |
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ) | |
| 497.9290 | 1490.7652 | 1490.7828 | -11.8 | 0 | 16 | 0.15 | 1Score > 41 indicates identityScore > 20 indicates homology |
TSALALSSLSGDIQK + Deamidated (NQ) | |
| 471.7761 | 941.5376 | 941.5546 | -18.0 | 0 | 16 | 0.84 | 1Score > 40 indicates identityScore > 28 indicates homology |
QGVVDLLAK | |
| 736.8677 | 1471.7208 | 1471.7381 | -11.7 | 0 | 16 | 0.69 | 1Score > 40 indicates identityScore > 27 indicates homology |
WALMIDPQGQALK + 2 Deamidated (NQ) | |
| 724.4366 | 2170.2880 | 2170.2911 | -1.46 | 0 | 16 | 0.51 | 1Score > 25 indicates identity |
LIIFAMNGVLVAIAGLILSAR + Oxidation (M) | |
| 653.6844 | 1958.0314 | 1958.0275 | 1.98 | 0 | 16 | 0.27 | 1Score > 38 indicates identityScore > 23 indicates homology |
LFPSTHALQPHLSAHFR | |
| 519.3005 | 1554.8797 | 1554.8558 | 15.3 | 1 | 16 | 0.67 | 1Score > 36 indicates identityScore > 27 indicates homology |
RFPVALALSAPVWE | |
| 566.2827 | 1130.5508 | 1130.5642 | -11.8 | 0 | 16 | 0.92 | 1Score > 41 indicates identityScore > 28 indicates homology |
VVIQDPMGQK + Deamidated (NQ); Oxidation (M) | |
| 307.1816 | 612.3486 | 612.3595 | -17.7 | 0 | 16 | 0.97 | 1Score > 38 indicates identityScore > 28 indicates homology |
IEPVR | |
| 494.2656 | 986.5166 | 986.5033 | 13.5 | 0 | 16 | 0.73 | 1Score > 42 indicates identityScore > 27 indicates homology |
GGEPGVSGTVK | |
| 712.3618 | 1422.7090 | 1422.7354 | -18.5 | 1 | 16 | 0.95 | 1Score > 41 indicates identityScore > 28 indicates homology |
LQSQIQSKFQSK + 2 Deamidated (NQ) | |
| 587.3525 | 586.3452 | 586.3439 | 2.33 | 0 | 16 | 0.57 | 1Score > 44 indicates identityScore > 26 indicates homology |
AGNVVK | |
| 637.8123 | 1273.6100 | 1273.6037 | 4.96 | 1 | 16 | 0.5 | 1Score > 41 indicates identityScore > 25 indicates homology |
DEINDALINQK + 2 Deamidated (NQ) | |
| 349.8419 | 1046.5039 | 1046.5179 | -13.4 | 0 | 16 | 0.76 | 1Score > 42 indicates identityScore > 27 indicates homology |
MVQNGTIAGR + Deamidated (NQ) | |
| 660.3894 | 1318.7642 | 1318.7497 | 11.1 | 1 | 16 | 0.68 | 1Score > 34 indicates identityScore > 27 indicates homology |
FLGTTKDPVTLK | |
| 836.4256 | 2506.2550 | 2506.2295 | 10.2 | 1 | 16 | 0.083 | 1Score > 38 indicates identityScore > 17 indicates homology |
VNTMLLSTSVSRLMQPADTPSLE + Deamidated (NQ); Oxidation (M) | |
| 380.7233 | 759.4320 | 759.4279 | 5.44 | 0 | 16 | 0.81 | 1Score > 40 indicates identityScore > 27 indicates homology |
LFDPLR | |
| 808.4290 | 1614.8434 | 1614.8729 | -18.2 | 1 | 16 | 0.4 | 1Score > 40 indicates identityScore > 24 indicates homology |
RNFLNQAASLLLPR + 3 Deamidated (NQ) | |
| 685.3321 | 2052.9745 | 2052.9851 | -5.19 | 0 | 16 | 0.34 | 1Score > 39 indicates identityScore > 24 indicates homology |
TNAVSLTNVYDQVSQGNIK + 3 Deamidated (NQ) | |
| 508.7704 | 1015.5262 | 1015.5338 | -7.45 | 1 | 16 | 0.72 | 1Score > 42 indicates identityScore > 27 indicates homology |
LPLEPEYR | |
| 748.8986 | 1495.7826 | 1495.8021 | -13.0 | 1 | 16 | 0.88 | 1Score > 39 indicates identityScore > 28 indicates homology |
IPIELLQVNDIVE + 2 Deamidated (NQ) | |
| 807.9485 | 1613.8824 | 1613.8988 | -10.2 | 1 | 16 | 0.81 | 1Score > 38 indicates identityScore > 27 indicates homology |
TGTVTPNVTKAVADIK | |
| 754.0705 | 2259.1897 | 2259.1575 | 14.2 | 1 | 16 | 0.11 | 1Score > 37 indicates identityScore > 18 indicates homology |
NLLSFLRGTFLNNYVPFDK + 2 Deamidated (NQ) | |
| 413.7434 | 825.4722 | 825.4821 | -11.9 | 1 | 16 | 0.97 | 1Score > 37 indicates identityScore > 28 indicates homology |
IPGIDRR | |
| 559.3245 | 1116.6344 | 1116.6251 | 8.35 | 1 | 16 | 0.24 | 1Score > 39 indicates identityScore > 22 indicates homology |
TGTVNVTIRR + Deamidated (NQ) | |
| 562.2984 | 1683.8734 | 1683.8501 | 13.8 | 1 | 16 | 0.49 | 1Score > 39 indicates identityScore > 25 indicates homology |
LSPAQISELMHLSDK + Oxidation (M) | |
| 705.3867 | 2113.1383 | 2113.1380 | 0.11 | 0 | 16 | 0.15 | 1Score > 36 indicates identityScore > 20 indicates homology |
WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ) | |
| 434.2941 | 866.5736 | 866.5701 | 4.04 | 0 | 16 | 0.094 | 1Score > 28 indicates identityScore > 18 indicates homology |
LGLKPLAR | |
| 804.8949 | 1607.7752 | 1607.7647 | 6.55 | 0 | 16 | 0.46 | 1Score > 41 indicates identityScore > 25 indicates homology |
LGADVMNMSVGNSSVK | |
| 307.8565 | 920.5477 | 920.5331 | 15.8 | 0 | 16 | 0.28 | 1Score > 35 indicates identityScore > 23 indicates homology |
QLYLSAVK | |
| 339.4994 | 1015.4764 | 1015.4822 | -5.72 | 1 | 16 | 0.51 | 1Score > 40 indicates identityScore > 25 indicates homology |
QPLNTGEQK + 2 Deamidated (NQ) | |
| 1030.5322 | 2059.0498 | 2059.0884 | -18.7 | 1 | 16 | 0.88 | 1Score > 39 indicates identityScore > 28 indicates homology |
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M) | |
| 833.4913 | 1664.9680 | 1664.9937 | -15.4 | 1 | 16 | 0.62 | 1Score > 32 indicates identityScore > 26 indicates homology |
LGIPLLKALQTQASGR | |
| 519.3012 | 1554.8818 | 1554.8558 | 16.7 | 1 | 16 | 0.76 | 1Score > 36 indicates identityScore > 27 indicates homology |
RFPVALALSAPVWE | |
| 586.3884 | 585.3811 | 585.3737 | 12.6 | 0 | 16 | 0.54 | 1Score > 39 indicates identityScore > 25 indicates homology |
ILDIL | |
| 806.9442 | 1611.8738 | 1611.8508 | 14.3 | 1 | 16 | 0.52 | 1Score > 38 indicates identityScore > 25 indicates homology |
YAQGIQKLASQYLK + 2 Deamidated (NQ) | |
| 423.7283 | 845.4420 | 845.4317 | 12.3 | 1 | 16 | 0.98 | 1Score > 42 indicates identityScore > 28 indicates homology |
IPDDKMK | |
| 397.7162 | 1586.8357 | 1586.8264 | 5.87 | 1 | 16 | 0.53 | 1Score > 40 indicates identityScore > 25 indicates homology |
VDDTIQTLQQRLR + 2 Deamidated (NQ) | |
| 639.0002 | 1913.9788 | 1913.9496 | 15.2 | 1 | 16 | 0.15 | 1Score > 39 indicates identityScore > 20 indicates homology |
YDLAIHAYNKNNSHVR | |
| 382.7206 | 763.4266 | 763.4228 | 5.03 | 1 | 16 | 0.5 | 1Score > 41 indicates identityScore > 25 indicates homology |
IIAEYR | |
| 904.4604 | 1806.9062 | 1806.8748 | 17.4 | 1 | 16 | 0.89 | 1Score > 40 indicates identityScore > 28 indicates homology |
GLQGEQGAPGLPGSQGPAGK + 2 Deamidated (NQ) | |
| 396.8768 | 1187.6086 | 1187.5975 | 9.31 | 1 | 16 | 0.53 | 1Score > 42 indicates identityScore > 25 indicates homology |
KGSFPSFGFSK | |
| 456.7625 | 911.5104 | 911.5116 | -1.31 | 1 | 15 | 0.96 | 1Score > 38 indicates identityScore > 28 indicates homology |
KIVDYFK | |
| 382.7331 | 763.4516 | 763.4480 | 4.82 | 0 | 15 | 0.41 | 1Score > 35 indicates identityScore > 24 indicates homology |
IIDYIK | |
| 554.2530 | 1106.4914 | 1106.4914 | 0.076 | 1 | 15 | 0.54 | 1Score > 40 indicates identityScore > 25 indicates homology |
VLDQMNENK + Deamidated (NQ); Oxidation (M) | |
| 629.8594 | 1257.7042 | 1257.6929 | 9.05 | 1 | 15 | 0.92 | 1Score > 39 indicates identityScore > 28 indicates homology |
VENVSTVNVLGK | |
| 710.0477 | 2127.1213 | 2127.0960 | 11.9 | 1 | 15 | 0.6 | 1Score > 38 indicates identityScore > 26 indicates homology |
AADTAGDLAGLEIAGPGFINVR | |
| 704.3743 | 1406.7340 | 1406.7075 | 18.9 | 0 | 15 | 0.31 | 1Score > 40 indicates identityScore > 23 indicates homology |
MLSSQVLTLDQR + Deamidated (NQ); Oxidation (M) | |
| 442.2543 | 882.4940 | 882.4923 | 1.99 | 1 | 15 | 0.8 | 1Score > 37 indicates identityScore > 27 indicates homology |
QIPIEQR | |
| 722.4155 | 1442.8164 | 1442.8093 | 4.98 | 0 | 15 | 0.25 | 1Score > 37 indicates identityScore > 22 indicates homology |
IAGAGAASVLSALTNK | |
| 400.7729 | 799.5312 | 799.5280 | 4.08 | 1 | 15 | 0.81 | 1Score > 36 indicates identityScore > 27 indicates homology |
TVRVVVK | |
| 336.7064 | 671.3982 | 671.4078 | -14.3 | 1 | 15 | 0.8 | 1Score > 43 indicates identityScore > 27 indicates homology |
ALQRGK | |
| 321.2036 | 640.3926 | 640.3908 | 2.87 | 0 | 15 | 0.88 | 1Score > 35 indicates identityScore > 27 indicates homology |
AVGPLGK | |
| 398.1992 | 794.3838 | 794.3810 | 3.57 | 1 | 15 | 0.49 | 1Score > 41 indicates identityScore > 25 indicates homology |
AVFENSK + Deamidated (NQ) | |
| 904.9625 | 1807.9104 | 1807.9203 | -5.47 | 0 | 15 | 0.42 | 1Score > 40 indicates identityScore > 24 indicates homology |
AQVDALLNSPNVDIPLE + Deamidated (NQ) | |
| 427.5948 | 1279.7626 | 1279.7387 | 18.6 | 0 | 15 | 0.61 | 1Score > 33 indicates identityScore > 26 indicates homology |
AVSILNDLPPLK + Deamidated (NQ) | |
| 407.2284 | 812.4422 | 812.4392 | 3.76 | 1 | 15 | 0.85 | 1Score > 39 indicates identityScore > 27 indicates homology |
AKAPPSDK | |
| 735.3920 | 2203.1542 | 2203.1888 | -15.7 | 1 | 15 | 0.29 | 1Score > 37 indicates identityScore > 23 indicates homology |
FSYAVQIPLNLELLVNNQK + Deamidated (NQ) | |
| 469.7328 | 937.4510 | 937.4505 | 0.57 | 0 | 15 | 0.47 | 1Score > 41 indicates identityScore > 25 indicates homology |
VLTGDYDR | |
| 922.4930 | 1842.9714 | 1842.9574 | 7.60 | 0 | 15 | 0.34 | 1Score > 39 indicates identityScore > 23 indicates homology |
TANGATIDQIAQLIDGLK + 2 Deamidated (NQ) | |
| 422.9206 | 1265.7400 | 1265.7417 | -1.38 | 0 | 15 | 0.24 | 1Score > 34 indicates identityScore > 22 indicates homology |
DLPLIGMPIAVK | |
| 1006.5280 | 2011.0414 | 2011.0473 | -2.93 | 1 | 15 | 0.67 | 1Score > 39 indicates identityScore > 26 indicates homology |
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ) | |
| 812.4244 | 2434.2514 | 2434.2315 | 8.18 | 1 | 15 | 0.11 | 1Score > 38 indicates identityScore > 18 indicates homology |
LLTHLDNLPQWPDQVKTMQR + 2 Deamidated (NQ) | |
| 365.2194 | 728.4242 | 728.4221 | 2.94 | 0 | 15 | 0.77 | 1Score > 42 indicates identityScore > 27 indicates homology |
SPVWLK | |
| 615.8409 | 1229.6672 | 1229.6438 | 19.1 | 1 | 15 | 0.99 | 1Score > 41 indicates identityScore > 28 indicates homology |
VLNAMATIPRE + Oxidation (M) | |
| 794.4061 | 1586.7976 | 1586.8093 | -7.34 | 1 | 15 | 0.53 | 1Score > 40 indicates identityScore > 25 indicates homology |
LKGFWGQDVAPIQE | |
| 378.1982 | 1131.5728 | 1131.5785 | -5.05 | 1 | 15 | 0.35 | 1Score > 42 indicates identityScore > 23 indicates homology |
RSPNDIQFR | |
| 554.2911 | 1659.8515 | 1659.8216 | 18.0 | 1 | 15 | 0.48 | 1Score > 41 indicates identityScore > 25 indicates homology |
TYDPDTLAPALDGRR | |
| 356.8546 | 1067.5420 | 1067.5499 | -7.41 | 0 | 15 | 0.96 | 1Score > 40 indicates identityScore > 28 indicates homology |
QHIDTLVLE + Deamidated (NQ) | |
| 579.3073 | 1156.6000 | 1156.6088 | -7.54 | 1 | 15 | 0.88 | 1Score > 40 indicates identityScore > 27 indicates homology |
QQIRQILQE + 2 Deamidated (NQ) | |
| 313.8355 | 938.4847 | 938.4821 | 2.69 | 1 | 15 | 0.79 | 1Score > 39 indicates identityScore > 27 indicates homology |
VPDPGEGLR |
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