MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1192 529.7973 1057.5800 1057.5992 -18.1 1 17 0.98 +1Score > 41 indicates identity
Score > 30 indicates homology
LARGSNLTAR
1786 666.8492 1331.6838 1331.6755 6.30 1 17 0.22 +1Score > 41 indicates identity
Score > 23 indicates homology
MSPATLLAELNR + Deamidated (NQ); Oxidation (M)
754 436.2531 870.4916 870.4923 -0.74 1 17 0.84 +1Score > 40 indicates identity
Score > 29 indicates homology
NQLEVLR
1435 586.8535 1171.6924 1171.6924 0.0034 1 17 0.53 +1Score > 36 indicates identity
Score > 27 indicates homology
QATKLTAGIAAK
1258 547.2817 1092.5488 1092.5564 -6.88 1 17 0.8 +1Score > 42 indicates identity
Score > 29 indicates homology
LAGEFTQATR
130 489.2801 488.2728 488.2707 4.37 0 17 0.23 +1Score > 44 indicates identity
Score > 23 indicates homology
AGVSR
1507 603.2935 1204.5724 1204.5684 3.39 1 17 0.83 +1Score > 41 indicates identity
Score > 29 indicates homology
RQQSTVNNGAK + 3 Deamidated (NQ)
1161 519.2874 1036.5602 1036.5553 4.79 0 17 0.3 +1Score > 39 indicates identity
Score > 24 indicates homology
LSVNNISYK
2471 825.9308 1649.8470 1649.8777 -18.6 0 17 0.86 +1Score > 40 indicates identity
Score > 29 indicates homology
AIQNSFFLLINDVR + Deamidated (NQ)
421 358.2038 714.3930 714.4024 -13.1 1 17 0.97 +1Score > 42 indicates identity
Score > 30 indicates homology
AIGGEIR
3008 653.6835 1958.0287 1957.9938 17.8 0 17 0.2 +1Score > 38 indicates identity
Score > 23 indicates homology
FLSQPFFVAAQFTGVDGK
2103 748.3287 1494.6428 1494.6297 8.83 1 17 0.5 +1Score > 38 indicates identity
Score > 27 indicates homology
AEPTEQSFMQPGR + 2 Deamidated (NQ); Oxidation (M)
2829 617.6293 1849.8661 1849.8880 -11.9 0 17 0.099 +1Score > 40 indicates identity
Score > 20 indicates homology
SGQIDFDVIIASPDAMR + Oxidation (M)
793 444.7148 887.4150 887.4209 -6.59 1 17 0.76 +1Score > 39 indicates identity
Score > 28 indicates homology
NNQAQRR + 2 Deamidated (NQ)
1860 686.3414 1370.6682 1370.6830 -10.8 0 17 0.51 +1Score > 40 indicates identity
Score > 27 indicates homology
NIINLNSGHLFE + Deamidated (NQ)
2284 524.9627 1571.8663 1571.8671 -0.55 0 17 0.28 +1Score > 38 indicates identity
Score > 24 indicates homology
IGQTSSVGIHYLVAK
2308 791.3893 1580.7640 1580.7426 13.6 1 17 0.71 +1Score > 41 indicates identity
Score > 28 indicates homology
SGVLKSNCIMDVLE + Deamidated (NQ); Oxidation (M)
2480 829.9368 1657.8590 1657.8410 10.9 0 17 0.2 +1Score > 40 indicates identity
Score > 23 indicates homology
SLIQNGADINVIASIE + 2 Deamidated (NQ)
632 408.2560 814.4974 814.4912 7.64 1 17 0.86 +1Score > 38 indicates identity
Score > 29 indicates homology
IEIATLR
2582 854.9536 1707.8926 1707.8679 14.5 1 17 0.91 +1Score > 39 indicates identity
Score > 29 indicates homology
QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
2214 772.4279 1542.8412 1542.8439 -1.73 0 17 0.64 +1Score > 39 indicates identity
Score > 28 indicates homology
NMVVSNILLNNIAK + Deamidated (NQ)
1437 587.2863 1172.5580 1172.5350 19.7 0 17 0.22 +1Score > 40 indicates identity
Score > 23 indicates homology
LGYNDTQFSK + Deamidated (NQ)
2255 781.4048 1560.7950 1560.8035 -5.43 0 17 0.74 +1Score > 41 indicates identity
Score > 28 indicates homology
LFILGGGSNILVNDE + Deamidated (NQ)
497 379.2515 756.4884 756.4745 18.4 1 17 0.51 +1Score > 39 indicates identity
Score > 27 indicates homology
VLVGEIK
359 344.6857 687.3568 687.3664 -13.9 0 17 0.43 +1Score > 45 indicates identity
Score > 26 indicates homology
QGAVTGR
2466 550.6288 1648.8646 1648.8533 6.86 1 17 0.42 +1Score > 39 indicates identity
Score > 26 indicates homology
LAELAQLQDQLDHR
1588 413.5606 1237.6600 1237.6601 -0.099 1 17 0.37 +1Score > 39 indicates identity
Score > 25 indicates homology
KLQLSGHAMPR + Deamidated (NQ)
192 301.1700 600.3254 600.3344 -14.8 0 17 0.99 +1Score > 39 indicates identity
Score > 29 indicates homology
QAGGLR
998 323.5236 967.5490 967.5451 4.03 0 17 0.45 +1Score > 36 indicates identity
Score > 26 indicates homology
QLAGVQVPR + Deamidated (NQ)
3174 687.3584 2059.0534 2059.0143 19.0 1 17 0.5 +1Score > 39 indicates identity
Score > 26 indicates homology
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
1299 560.7933 1119.5720 1119.5924 -18.2 1 17 0.61 +1Score > 42 indicates identity
Score > 27 indicates homology
QFLNEILSR + Deamidated (NQ)
1467 593.3488 1184.6830 1184.6652 15.0 1 17 0.64 +1Score > 38 indicates identity
Score > 27 indicates homology
VVAAQLVQEVK + 2 Deamidated (NQ)
1586 413.2142 1236.6208 1236.6271 -5.15 1 17 0.45 +1Score > 41 indicates identity
Score > 26 indicates homology
SLVQLSTMETK + Deamidated (NQ)
1563 410.2287 1227.6643 1227.6823 -14.7 1 17 0.54 +1Score > 40 indicates identity
Score > 27 indicates homology
QPDSQVTVKVK
2299 790.4513 1578.8880 1578.8729 9.58 1 17 0.62 +1Score > 35 indicates identity
Score > 27 indicates homology
ELQANVVHLSLLSR + Deamidated (NQ)
2784 908.4423 1814.8700 1814.9050 -19.3 1 17 0.38 +1Score > 40 indicates identity
Score > 25 indicates homology
KSSDVFSTIDSIFQNK
603 400.7132 799.4118 799.4188 -8.67 1 17 0.25 +1Score > 39 indicates identity
Score > 23 indicates homology
VNPRANK + 2 Deamidated (NQ)
2843 619.6652 1855.9738 1855.9891 -8.26 0 17 0.33 +1Score > 39 indicates identity
Score > 25 indicates homology
VLTSDSNIVPVDISGIAR + Deamidated (NQ)
857 457.7751 913.5356 913.5233 13.5 1 17 0.83 +1Score > 40 indicates identity
Score > 29 indicates homology
QNKTVVPK + Deamidated (NQ)
1336 378.5462 1132.6168 1132.6241 -6.43 1 17 0.73 +1Score > 40 indicates identity
Score > 28 indicates homology
LFQTVAEGLR
2577 569.9908 1706.9506 1706.9567 -3.57 0 17 0.27 +1Score > 36 indicates identity
Score > 24 indicates homology
LQNGLTPLLIVTPNGR + 2 Deamidated (NQ)
4452 1042.1724 3123.4954 3123.5320 -11.7 1 17 0.064 +1Score > 36 indicates identity
Score > 17 indicates homology
TNSTTTIINNNTQNNFTNITNIIVSKNE + Deamidated (NQ)
371 347.6808 693.3470 693.3446 3.57 0 17 0.65 +1Score > 42 indicates identity
Score > 27 indicates homology
AIYGDR
3290 710.0471 2127.1195 2127.0960 11.0 1 17 0.61 +1Score > 38 indicates identity
Score > 27 indicates homology
AADTAGDLAGLEIAGPGFINVR
458 365.6972 729.3798 729.3770 3.97 0 17 0.84 +1Score > 42 indicates identity
Score > 28 indicates homology
VGGIDNR
2607 574.2903 1719.8491 1719.8315 10.2 1 17 0.54 +1Score > 41 indicates identity
Score > 27 indicates homology
GTYDAISLNPDNAIEK
3069 667.0037 1997.9893 1997.9656 11.9 0 17 0.32 +1Score > 40 indicates identity
Score > 24 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
309 333.6777 665.3408 665.3384 3.62 0 17 0.95 +1Score > 41 indicates identity
Score > 29 indicates homology
DSFIGK
1195 530.3185 1058.6224 1058.6376 -14.3 1 17 0.78 +1Score > 36 indicates identity
Score > 28 indicates homology
EILSLIFPK
543 388.2328 774.4510 774.4487 2.99 0 17 0.85 +1Score > 39 indicates identity
Score > 28 indicates homology
TVDTVIK
2876 938.9557 1875.8968 1875.9335 -19.5 1 17 0.26 +1Score > 40 indicates identity
Score > 23 indicates homology
AIKNMMHQAYQLTQAK + Deamidated (NQ)
1498 601.3412 1200.6678 1200.6601 6.42 1 17 0.76 +1Score > 40 indicates identity
Score > 28 indicates homology
DLVENAISLVK + Deamidated (NQ)
2205 769.9131 1537.8116 1537.8212 -6.24 0 17 0.46 +1Score > 39 indicates identity
Score > 26 indicates homology
TTLPSQGPLQRPSR + Deamidated (NQ)
2501 834.9508 1667.8870 1667.8843 1.66 0 17 0.65 +1Score > 39 indicates identity
Score > 27 indicates homology
QGVDLVDVSSGAVVPAR
828 301.1580 900.4522 900.4413 12.1 1 17 0.82 +1Score > 41 indicates identity
Score > 28 indicates homology
QNENIGAR
1103 507.8136 1013.6126 1013.6233 -10.5 0 17 0.76 +1Score > 37 indicates identity
Score > 28 indicates homology
LTATINILR
2440 817.9329 1633.8512 1633.8715 -12.4 0 17 0.42 +1Score > 40 indicates identity
Score > 25 indicates homology
DLLVSYDIAIPPYR
1158 518.2990 1034.5834 1034.5648 18.1 1 17 0.6 +1Score > 38 indicates identity
Score > 27 indicates homology
AIIKYQGLE + Deamidated (NQ)
2469 825.9291 1649.8436 1649.8624 -11.4 0 17 0.073 +1Score > 40 indicates identity
Score > 18 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
1917 700.3311 1398.6476 1398.6514 -2.69 0 17 0.98 +1Score > 40 indicates identity
Score > 29 indicates homology
IIHQITDLNNSE + 3 Deamidated (NQ)
2665 879.9924 1757.9702 1757.9563 7.93 1 17 0.25 +1Score > 36 indicates identity
Score > 23 indicates homology
KLNSAGINILPQLTFE + Deamidated (NQ)
3754 794.1028 2379.2866 2379.2885 -0.83 0 17 0.097 +1Score > 35 indicates identity
Score > 19 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1385 385.2253 1152.6541 1152.6363 15.4 0 17 0.45 +1Score > 37 indicates identity
Score > 26 indicates homology
TLRPAGAPTNR
2105 748.3682 1494.7218 1494.7355 -9.11 1 17 0.42 +1Score > 41 indicates identity
Score > 25 indicates homology
EQVSSPFTISSWK
2158 379.2159 1512.8345 1512.8512 -11.0 0 17 0.43 +1Score > 38 indicates identity
Score > 25 indicates homology
TSGDLLAGLLLDGLR
2134 501.9202 1502.7388 1502.7148 16.0 0 17 0.35 +1Score > 41 indicates identity
Score > 24 indicates homology
MTQVGQTHTSGSIR + Deamidated (NQ)
2444 545.6333 1633.8781 1633.9053 -16.6 1 17 0.31 +1Score > 38 indicates identity
Score > 24 indicates homology
APQFTGGGIVHGPKLR
3753 794.1021 2379.2845 2379.2885 -1.71 0 17 0.3 +1Score > 35 indicates identity
Score > 24 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2612 575.2921 1722.8545 1722.8577 -1.87 0 17 0.33 +1Score > 41 indicates identity
Score > 24 indicates homology
LSAPTAIQFYDVQDR
2770 603.9519 1808.8339 1808.8145 10.7 1 17 0.6 +1Score > 40 indicates identity
Score > 27 indicates homology
GQNITMRGQDMSAATAR + 2 Deamidated (NQ)
1172 348.5516 1042.6330 1042.6135 18.7 1 16 0.43 +1Score > 36 indicates identity
Score > 25 indicates homology
LKALGATNVR + Deamidated (NQ)
465 366.7072 731.3998 731.4038 -5.44 1 16 0.96 +1Score > 41 indicates identity
Score > 29 indicates homology
VGSRASR
2099 747.8839 1493.7532 1493.7435 6.50 1 16 0.67 +1Score > 41 indicates identity
Score > 27 indicates homology
YIDNKITMAIPAE + Oxidation (M)
1704 648.3776 1294.7406 1294.7609 -15.6 1 16 0.3 +1Score > 35 indicates identity
Score > 24 indicates homology
VVGLSTQLPPRK + Deamidated (NQ)
2061 490.9315 1469.7727 1469.7772 -3.10 1 16 0.2 +1Score > 40 indicates identity
Score > 22 indicates homology
MNAQPRSALALAAR + Deamidated (NQ)
2100 747.8843 1493.7540 1493.7362 12.0 0 16 0.87 +1Score > 41 indicates identity
Score > 28 indicates homology
QVTLLGQNVNSYR + 3 Deamidated (NQ)
2032 485.9592 1454.8558 1454.8708 -10.3 1 16 0.74 +1Score > 33 indicates identity
Score > 28 indicates homology
GVASEIVVNTLLIK
2098 747.8836 1493.7526 1493.7435 6.09 1 16 0.66 +1Score > 41 indicates identity
Score > 27 indicates homology
YIDNKITMAIPAE + Oxidation (M)
542 388.2328 774.4510 774.4487 3.03 0 16 0.29 +1Score > 39 indicates identity
Score > 24 indicates homology
ISLTVNK + Deamidated (NQ)
2176 761.4244 1520.8342 1520.8100 16.0 1 16 0.15 +1Score > 37 indicates identity
Score > 21 indicates homology
AQGLRSVFVSGFPR + Deamidated (NQ)
213 305.1721 608.3296 608.3282 2.38 0 16 0.36 +1Score > 38 indicates identity
Score > 25 indicates homology
YGTIR
1302 560.7959 1119.5772 1119.5772 0.062 1 16 0.95 +1Score > 42 indicates identity
Score > 29 indicates homology
SSVTVSVKGQE
2203 769.8900 1537.7654 1537.7511 9.32 1 16 0.66 +1Score > 40 indicates identity
Score > 27 indicates homology
YNIDSIQSEINLK + 2 Deamidated (NQ)
313 335.1913 668.3680 668.3646 5.19 0 16 0.76 +1Score > 34 indicates identity
Score > 28 indicates homology
FYIAR
1932 468.9148 1403.7226 1403.6946 19.9 0 16 0.3 +1Score > 41 indicates identity
Score > 24 indicates homology
NFHQHQITHLK + 2 Deamidated (NQ)
2378 538.2921 1611.8545 1611.8580 -2.19 1 16 0.26 +1Score > 39 indicates identity
Score > 23 indicates homology
LSARPGNEVNLLDSK
941 473.2808 944.5470 944.5443 2.86 0 16 0.24 +1Score > 40 indicates identity
Score > 23 indicates homology
IYPGQVLR
1232 541.7523 1081.4900 1081.4783 10.8 1 16 0.38 +1Score > 40 indicates identity
Score > 25 indicates homology
EITSNMMQK + Deamidated (NQ)
321 336.2202 670.4258 670.4126 19.8 1 16 0.95 +1Score > 40 indicates identity
Score > 29 indicates homology
RVTAPK
1651 633.8816 1265.7486 1265.7343 11.3 0 16 0.74 +1Score > 33 indicates identity
Score > 28 indicates homology
LQLQQLGLVPR + 2 Deamidated (NQ)
3366 721.3852 2161.1338 2161.1406 -3.15 1 16 0.13 +1Score > 38 indicates identity
Score > 20 indicates homology
FTVLDDVIEDVVTILNNLK + 2 Deamidated (NQ)
2002 718.9130 1435.8114 1435.7922 13.4 1 16 0.49 +1Score > 35 indicates identity
Score > 26 indicates homology
AISKILDAISDYK
903 464.7278 927.4410 927.4549 -14.9 1 16 0.36 +1Score > 40 indicates identity
Score > 24 indicates homology
TLSYETSK
3427 730.3796 2188.1170 2188.1198 -1.28 1 16 0.081 +1Score > 38 indicates identity
Score > 18 indicates homology
INAAHGFSLIQVDNTKVTMK + 2 Deamidated (NQ)
1685 644.3580 1286.7014 1286.6983 2.48 1 16 0.83 +1Score > 40 indicates identity
Score > 28 indicates homology
IIIGLWNSSRE
2127 501.2708 1500.7906 1500.8147 -16.1 1 16 0.19 +1Score > 40 indicates identity
Score > 22 indicates homology
QELQALTAALSASAK
2192 510.9531 1529.8375 1529.8123 16.5 0 16 0.46 +1Score > 39 indicates identity
Score > 25 indicates homology
ASPRPILMLSSLTE + Oxidation (M)
2810 919.9567 1837.8988 1837.9172 -9.98 1 16 0.26 +1Score > 40 indicates identity
Score > 23 indicates homology
VYSMFKSVILATFTSE + Oxidation (M)
1191 529.7924 1057.5702 1057.5655 4.47 0 16 0.51 +1Score > 42 indicates identity
Score > 26 indicates homology
LAIIGSDIQE
2508 835.0083 1668.0020 1667.9974 2.78 1 16 0.16 +1Score > 28 indicates identity
Score > 21 indicates homology
DPKIHGILVQLPLPK + Deamidated (NQ)
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