MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1531 304.6744 1214.6685 1214.6619 5.46 1 19 0.4 +1Score > 40 indicates identity
Score > 27 indicates homology		 ALIQSAIGSAER
587 397.2049 792.3952 792.3878 9.38 1 19 0.34 +1Score > 42 indicates identity
Score > 26 indicates homology		 RNAQFR + 2 Deamidated (NQ)
1160 519.2746 1036.5346 1036.5488 -13.6 1 19 0.28 +1Score > 41 indicates identity
Score > 26 indicates homology		 LAAAFKMNR + Oxidation (M)
1903 464.2618 1389.7636 1389.7504 9.51 1 19 0.24 +1Score > 39 indicates identity
Score > 25 indicates homology		 GLFVAISNQEIAK + Deamidated (NQ)
2320 793.4349 1584.8552 1584.8246 19.3 1 19 0.48 +1Score > 39 indicates identity
Score > 28 indicates homology		 DKGALDNLLAQLIAE + 2 Deamidated (NQ)
396 351.7066 701.3986 701.3959 3.87 0 19 0.61 +1Score > 43 indicates identity
Score > 29 indicates homology		 LISIAGE
1724 435.8963 1304.6671 1304.6725 -4.13 0 19 0.6 +1Score > 41 indicates identity
Score > 29 indicates homology		 NLLASFGDNVVR + Deamidated (NQ)
1055 497.7726 993.5306 993.5165 14.3 1 19 0.87 +1Score > 40 indicates identity
Score > 30 indicates homology		 GTISKNMVK + Deamidated (NQ); Oxidation (M)
1124 510.7577 1019.5008 1019.4957 5.02 1 19 0.98 +1Score > 42 indicates identity
Score > 31 indicates homology		 VQSAIEMDK
1702 431.9081 1292.7025 1292.7128 -8.02 1 19 0.11 +1Score > 39 indicates identity
Score > 21 indicates homology		 QLPFDKYLLR + Deamidated (NQ)
959 317.8358 950.4856 950.4855 0.072 1 19 0.81 +1Score > 41 indicates identity
Score > 30 indicates homology		 NGMSITKGK + Oxidation (M)
2796 365.7979 1823.9531 1823.9460 3.90 1 18 0.12 +1Score > 39 indicates identity
Score > 22 indicates homology		 FMKVDVLGLGMLGCLR + Oxidation (M)
1773 664.3871 1326.7596 1326.7659 -4.75 1 18 0.52 +1Score > 37 indicates identity
Score > 28 indicates homology		 ISLDHLIFNKK
2443 817.9393 1633.8640 1633.8423 13.3 1 18 0.072 +1Score > 39 indicates identity
Score > 20 indicates homology		 LNAAHPIEQVGSQLR + 2 Deamidated (NQ)
2253 780.4405 1558.8664 1558.8687 -1.44 1 18 0.095 +1Score > 37 indicates identity
Score > 21 indicates homology		 KSLCANILMIKPR + Oxidation (M)
496 379.2332 756.4518 756.4646 -16.9 0 18 0.47 +1Score > 41 indicates identity
Score > 28 indicates homology		 KPLFPR
735 430.7481 859.4816 859.4949 -15.4 1 18 0.94 +1Score > 42 indicates identity
Score > 31 indicates homology		 LKMNLNK
749 434.2906 866.5666 866.5589 8.91 0 18 0.28 +1Score > 29 indicates identity
Score > 25 indicates homology		 IGGLPAVLK
1692 645.3503 1288.6860 1288.6636 17.4 1 18 0.36 +1Score > 41 indicates identity
Score > 26 indicates homology		 FGRGGNNLVQAR + Deamidated (NQ)
1547 408.1898 1221.5476 1221.5547 -5.84 0 18 0.47 +1Score > 40 indicates identity
Score > 28 indicates homology		 DQDILNMVTR + 2 Deamidated (NQ); Oxidation (M)
275 322.2118 642.4090 642.4064 4.07 1 18 1 +1Score > 41 indicates identity
Score > 31 indicates homology		 IAKLAQ
1025 488.7847 975.5548 975.5614 -6.69 1 18 0.65 +1Score > 39 indicates identity
Score > 29 indicates homology		 LYQQRIR
1526 304.4161 1213.6353 1213.6204 12.3 1 18 0.64 +1Score > 40 indicates identity
Score > 29 indicates homology		 KNVFTPHSAGR + Deamidated (NQ)
143 516.3042 515.2969 515.2955 2.79 0 18 0.16 +1Score > 47 indicates identity
Score > 23 indicates homology		 ALAQL + Deamidated (NQ)
1472 594.3452 1186.6758 1186.6822 -5.37 0 18 0.52 +1Score > 39 indicates identity
Score > 28 indicates homology		 LGLGILNNAFR
1495 600.3407 1198.6668 1198.6670 -0.095 1 18 0.84 +1Score > 40 indicates identity
Score > 30 indicates homology		 GRNAIISLQVE
3794 806.4198 2416.2376 2416.2611 -9.75 1 18 0.65 +1Score > 38 indicates identity
Score > 29 indicates homology		 GYDAPRPGLDFRLLITGGSQGAR
451 365.1993 728.3840 728.3817 3.25 0 18 0.62 +1Score > 39 indicates identity
Score > 29 indicates homology		 LAADPSR
906 465.2786 928.5426 928.5454 -2.94 1 18 0.66 +1Score > 40 indicates identity
Score > 29 indicates homology		 IINSARQK
1113 508.7646 1015.5146 1015.5186 -3.85 0 18 0.91 +1Score > 42 indicates identity
Score > 30 indicates homology		 IANLGNLGDK + 2 Deamidated (NQ)
1852 456.5706 1366.6900 1366.6915 -1.11 0 18 0.43 +1Score > 40 indicates identity
Score > 27 indicates homology		 GTYVCIATNALGK
1682 321.1858 1280.7141 1280.7340 -15.5 1 18 0.31 +1Score > 37 indicates identity
Score > 26 indicates homology		 LAELNPIVDAVK
2008 720.8439 1439.6732 1439.6933 -13.9 1 18 0.26 +1Score > 40 indicates identity
Score > 25 indicates homology		 QGDIFVVGEQYGK + Deamidated (NQ)
197 301.6801 601.3456 601.3547 -15.1 1 18 0.67 +1Score > 45 indicates identity
Score > 29 indicates homology		 AGAKGAK
1293 558.3104 1114.6062 1114.6022 3.59 1 18 0.77 +1Score > 41 indicates identity
Score > 30 indicates homology		 LPGYPVNEVK
1904 464.2622 1389.7648 1389.7504 10.4 1 18 0.85 +1Score > 38 indicates identity
Score > 30 indicates homology		 GLFVAISNQEIAK + Deamidated (NQ)
2976 967.4996 1932.9846 1933.0165 -16.5 1 18 0.16 +1Score > 40 indicates identity
Score > 23 indicates homology		 YNITILMITHEMSVIR
286 324.6733 647.3320 647.3312 1.27 1 18 0.52 +1Score > 42 indicates identity
Score > 28 indicates homology		 IMEGAK
3259 706.0616 2115.1630 2115.1398 11.0 1 18 0.25 +1Score > 35 indicates identity
Score > 25 indicates homology		 MPNTNPLFIDALASVVLKR + Deamidated (NQ); Oxidation (M)
340 338.1853 674.3560 674.3599 -5.70 0 18 0.77 +1Score > 43 indicates identity
Score > 29 indicates homology		 ATTASPK
758 437.2587 872.5028 872.5154 -14.3 0 18 0.92 +1Score > 41 indicates identity
Score > 30 indicates homology		 VLVACAIK
1608 621.4038 1240.7930 1240.8118 -15.1 1 18 0.23 +1Score > 24 indicates identity KISISLIGGLLK
397 351.7083 701.4020 701.3959 8.69 0 18 0.93 +1Score > 43 indicates identity
Score > 30 indicates homology		 IIVDDK
481 372.7229 743.4312 743.4290 3.06 0 18 0.37 +1Score > 43 indicates identity
Score > 26 indicates homology		 LTNAGLR
1913 466.2608 1395.7606 1395.7650 -3.14 1 18 0.16 +1Score > 38 indicates identity
Score > 23 indicates homology		 LDGYDIISLIKF
2553 847.4175 1692.8204 1692.8029 10.4 0 18 0.37 +1Score > 41 indicates identity
Score > 26 indicates homology		 DFASLAAANPMSAIVSE
2091 744.9072 1487.7998 1487.8235 -15.9 1 18 0.12 +1Score > 39 indicates identity
Score > 21 indicates homology		 ILDFGIAKLVQAAE + Deamidated (NQ)
246 315.1856 628.3566 628.3544 3.55 0 18 0.68 +1Score > 37 indicates identity
Score > 29 indicates homology		 AVNTPK
1744 440.2211 1317.6415 1317.6486 -5.39 1 18 0.76 +1Score > 42 indicates identity
Score > 29 indicates homology		 NLMEDILNDLK + Deamidated (NQ)
976 479.7839 957.5532 957.5495 3.91 0 18 0.81 +1Score > 41 indicates identity
Score > 30 indicates homology		 QIVGSDLVK
1004 486.2740 970.5334 970.5447 -11.6 0 18 0.92 +1Score > 41 indicates identity
Score > 30 indicates homology		 IGAATVNTPK
1548 611.8112 1221.6078 1221.6023 4.53 1 18 0.79 +1Score > 41 indicates identity
Score > 29 indicates homology		 QKGLSSAMLDR + Deamidated (NQ); Oxidation (M)
565 392.7707 783.5268 783.5218 6.44 0 18 0.55 +1Score > 32 indicates identity
Score > 28 indicates homology		 IIAQIVK
1722 435.2616 1302.7630 1302.7547 6.34 1 18 0.98 +1Score > 34 indicates identity
Score > 30 indicates homology		 VLVINFKDLNK + Deamidated (NQ)
2699 591.3211 1770.9415 1770.9702 -16.2 0 18 0.32 +1Score > 38 indicates identity
Score > 25 indicates homology		 TLFNMLGPLLNPAQVK + Oxidation (M)
875 460.7481 919.4816 919.4797 2.13 0 18 0.96 +1Score > 42 indicates identity
Score > 30 indicates homology		 IGNMVLTR + Deamidated (NQ); Oxidation (M)
1431 586.8326 1171.6506 1171.6635 -10.9 0 18 0.79 +1Score > 40 indicates identity
Score > 29 indicates homology		 VAALTMASAPLK
1954 708.3636 1414.7126 1414.7245 -8.36 0 18 0.79 +1Score > 40 indicates identity
Score > 29 indicates homology		 LIWGVYPANEPR + Deamidated (NQ)
1236 542.7886 1083.5626 1083.5560 6.11 0 18 0.31 +1Score > 40 indicates identity
Score > 25 indicates homology		 GSAPGQPVTAAK + Deamidated (NQ)
1963 710.8428 1419.6710 1419.6738 -1.90 1 18 0.55 +1Score > 41 indicates identity
Score > 28 indicates homology		 MQETALLNMVPR + 2 Deamidated (NQ); Oxidation (M)
3467 735.0670 2202.1792 2202.1354 19.9 1 18 0.14 +1Score > 36 indicates identity
Score > 22 indicates homology		 LFDMVETLSHNNNVSILLK + Oxidation (M)
1423 584.8618 1167.7090 1167.6976 9.83 0 18 0.15 +1Score > 34 indicates identity
Score > 22 indicates homology		 LGAAVVVDVVAR
2145 504.6069 1510.7989 1510.7932 3.73 1 18 0.3 +1Score > 39 indicates identity
Score > 25 indicates homology		 VDFPFVQRYLAR + Deamidated (NQ)
1997 717.8641 1433.7136 1433.6899 16.6 0 18 0.35 +1Score > 41 indicates identity
Score > 26 indicates homology		 QIQIYNVNNGNR + 2 Deamidated (NQ)
405 352.2226 702.4306 702.4276 4.36 0 18 0.38 +1Score > 38 indicates identity
Score > 26 indicates homology		 VAATTIK
979 479.8029 957.5912 957.5859 5.63 0 18 0.59 +1Score > 34 indicates identity
Score > 28 indicates homology		 LLSGISIAGK
1523 405.2044 1212.5914 1212.5696 18.0 0 18 0.21 +1Score > 40 indicates identity
Score > 24 indicates homology		 MLYQQIASNK + 2 Deamidated (NQ); Oxidation (M)
922 470.3012 938.5878 938.5913 -3.64 1 18 0.75 +1Score > 32 indicates identity
Score > 29 indicates homology		 LLRAEPLK
1996 716.3865 1430.7584 1430.7477 7.48 1 18 0.23 +1Score > 40 indicates identity
Score > 24 indicates homology		 QLDNDLTRISTR
1292 372.5354 1114.5844 1114.5805 3.50 0 18 0.36 +1Score > 41 indicates identity
Score > 26 indicates homology		 LVMQQAGVPR + Deamidated (NQ); Oxidation (M)
2986 971.5312 1941.0478 1941.0492 -0.71 1 18 0.34 +1Score > 37 indicates identity
Score > 26 indicates homology		 MVLLDPLADALSIIKNAE + Oxidation (M)
3142 680.7233 2039.1481 2039.1415 3.22 1 18 0.53 +1Score > 33 indicates identity
Score > 27 indicates homology		 TYLVTVAPLESALLLSGHR
1532 608.3610 1214.7074 1214.6982 7.58 1 18 0.35 +1Score > 36 indicates identity
Score > 26 indicates homology		 TIQNLSRIIR + 2 Deamidated (NQ)
2376 538.2914 1611.8524 1611.8580 -3.49 1 18 0.33 +1Score > 39 indicates identity
Score > 25 indicates homology		 LSARPGNEVNLLDSK
1184 527.8345 1053.6544 1053.6434 10.5 0 18 0.074 +1Score > 33 indicates identity
Score > 19 indicates homology		 VAEPLITLAK
1151 517.7530 1033.4914 1033.4750 15.9 1 18 0.5 +1Score > 42 indicates identity
Score > 27 indicates homology		 NVEMALNVE + Oxidation (M)
2581 854.9532 1707.8918 1707.8679 14.0 1 18 0.57 +1Score > 39 indicates identity
Score > 28 indicates homology		 QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
1602 414.2370 1239.6892 1239.6823 5.55 1 18 0.76 +1Score > 38 indicates identity
Score > 29 indicates homology		 RGADILLVGTPE
1615 623.3181 1244.6216 1244.6361 -11.6 1 18 0.73 +1Score > 41 indicates identity
Score > 29 indicates homology		 QTQNAEGGIISK
610 401.2498 800.4850 800.4909 -7.26 0 18 0.46 +1Score > 40 indicates identity
Score > 27 indicates homology		 VVLAFPR
2347 533.6331 1597.8775 1597.8675 6.22 0 18 0.093 +1Score > 38 indicates identity
Score > 20 indicates homology		 TVADLGSGNGSPGIILK
508 380.7343 759.4540 759.4490 6.60 1 18 0.91 +1Score > 38 indicates identity
Score > 30 indicates homology		 LGKSIDK
2764 904.4631 1806.9116 1806.9220 -5.70 0 18 0.39 +1Score > 40 indicates identity
Score > 26 indicates homology		 INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
1645 632.8168 1263.6190 1263.6095 7.53 0 18 0.67 +1Score > 41 indicates identity
Score > 28 indicates homology		 DNNLIDVTFGR + Deamidated (NQ)
806 447.7392 893.4638 893.4607 3.55 0 18 0.52 +1Score > 41 indicates identity
Score > 27 indicates homology		 VTGLGQYR + Deamidated (NQ)
1100 506.3135 1010.6124 1010.6012 11.2 0 18 0.43 +1Score > 33 indicates identity
Score > 26 indicates homology		 INALQLIPK + 2 Deamidated (NQ)
1153 345.8189 1034.4349 1034.4516 -16.2 0 18 0.89 +1Score > 37 indicates identity
Score > 30 indicates homology		 SSGIDSVNQE
1135 343.1930 1026.5572 1026.5498 7.15 1 17 0.16 +1Score > 39 indicates identity
Score > 22 indicates homology		 RVYDTFVK
1759 331.1995 1320.7689 1320.7765 -5.77 1 17 0.23 +1Score > 33 indicates identity
Score > 24 indicates homology		 KPTSHILVEVAK
716 428.7310 855.4474 855.4450 2.85 1 17 0.89 +1Score > 39 indicates identity
Score > 29 indicates homology		 VGENALPR + Deamidated (NQ)
1326 565.8533 1129.6920 1129.6819 9.00 1 17 0.13 +1Score > 31 indicates identity
Score > 21 indicates homology		 LLGIVSSRSAK
1186 528.2845 1054.5544 1054.5447 9.21 0 17 0.76 +1Score > 40 indicates identity
Score > 29 indicates homology		 LGGYLYDVR
880 461.2584 920.5022 920.4967 6.02 1 17 0.71 +1Score > 40 indicates identity
Score > 28 indicates homology		 IAVAYQKE
3737 789.4036 2365.1890 2365.1584 12.9 1 17 0.29 +1Score > 38 indicates identity
Score > 25 indicates homology		 DGLPMTLPVRLGADADSANPGGPK + Deamidated (NQ); Oxidation (M)
1290 557.8315 1113.6484 1113.6393 8.19 0 17 0.82 +1Score > 39 indicates identity
Score > 29 indicates homology		 IANVLLASNAK + Deamidated (NQ)
244 314.7117 627.4088 627.4068 3.29 0 17 0.68 +1Score > 38 indicates identity
Score > 28 indicates homology		 IGLGLR
2159 379.2168 1512.8381 1512.8260 7.99 0 17 0.66 +1Score > 38 indicates identity
Score > 28 indicates homology		 LASNVNLSLGVVDGR
1898 695.8287 1389.6428 1389.6307 8.76 0 17 0.69 +1Score > 40 indicates identity
Score > 28 indicates homology		 QGSDIDAACGQLR
943 316.1798 945.5176 945.5243 -7.16 1 17 0.33 +1Score > 41 indicates identity
Score > 25 indicates homology		 KQTGVSAVR + Deamidated (NQ)
2587 570.6518 1708.9336 1708.9182 9.01 1 17 0.28 +1Score > 37 indicates identity
Score > 24 indicates homology		 VTNYMALDGLLSKLR + Oxidation (M)
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