MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1757 661.3392 1320.6638 1320.6714 -5.71 0 20 0.86 +1Score > 41 indicates identity
Score > 32 indicates homology		 YVANLTNLVWE
235 311.6826 621.3506 621.3486 3.27 0 20 0.57 +1Score > 38 indicates identity
Score > 30 indicates homology		 ATGFVK
715 428.2517 854.4888 854.4974 -10.00 1 20 0.61 +1Score > 37 indicates identity
Score > 31 indicates homology		 IPVDRQK
379 350.7044 699.3942 699.3915 3.91 0 20 0.43 +1Score > 39 indicates identity
Score > 29 indicates homology		 SPIINR + Deamidated (NQ)
208 603.3490 602.3417 602.3388 4.92 0 20 0.79 +1Score > 42 indicates identity
Score > 32 indicates homology		 IDISR
1496 601.3386 1200.6626 1200.6437 15.7 0 20 0.28 +1Score > 40 indicates identity
Score > 27 indicates homology		 MLNVVWNAVR
1865 343.9513 1371.7761 1371.7914 -11.2 0 20 0.28 +1Score > 37 indicates identity
Score > 27 indicates homology		 IWQAYAALLPVK
779 440.7283 879.4420 879.4450 -3.36 1 20 0.51 +1Score > 41 indicates identity
Score > 30 indicates homology		 TEPYRSK
1789 667.8596 1333.7046 1333.6990 4.24 1 20 0.2 +1Score > 40 indicates identity
Score > 26 indicates homology		 SFLVNLDRNQK + Deamidated (NQ)
1364 571.8629 1141.7112 1141.7070 3.68 0 20 0.61 +1Score > 30 indicates identity ISIQVLQTLK
2811 919.9572 1837.8998 1837.8767 12.6 0 20 0.87 +1Score > 40 indicates identity
Score > 32 indicates homology		 FQSSQILAMLNGKPSSE + 2 Deamidated (NQ)
3425 547.7715 2187.0569 2187.0956 -17.7 1 20 0.064 +1Score > 39 indicates identity
Score > 21 indicates homology		 DGSSKILFIYICPMQATVK + Deamidated (NQ); Oxidation (M)
459 365.7007 729.3868 729.3769 13.6 0 20 0.95 +1Score > 41 indicates identity
Score > 32 indicates homology		 INNNVR + Deamidated (NQ)
2593 571.6683 1711.9831 1711.9608 13.0 1 20 0.22 +1Score > 34 indicates identity
Score > 26 indicates homology		 QQPITTTLELVDIIK + Deamidated (NQ)
225 308.6876 615.3606 615.3592 2.42 1 20 0.92 +1Score > 45 indicates identity
Score > 32 indicates homology		 AIEVGK
296 328.6912 655.3678 655.3653 3.85 0 20 0.33 +1Score > 37 indicates identity
Score > 28 indicates homology		 IGDPVR
473 368.7284 735.4422 735.4391 4.22 0 20 0.87 +1Score > 34 indicates identity
Score > 32 indicates homology		 PLTHIR
1354 380.8909 1139.6509 1139.6299 18.4 1 20 0.52 +1Score > 37 indicates identity
Score > 29 indicates homology		 QQGKASTPPVK
620 404.2129 806.4112 806.4174 -7.65 1 20 0.85 +1Score > 41 indicates identity
Score > 32 indicates homology		 VKTFSPE
977 320.1920 957.5542 957.5607 -6.83 1 20 0.89 +1Score > 41 indicates identity
Score > 32 indicates homology		 KIVSIDAGR
1283 554.7885 1107.5624 1107.5812 -16.9 0 20 0.57 +1Score > 41 indicates identity
Score > 30 indicates homology		 YNTLIDLQK + Deamidated (NQ)
2131 501.6007 1501.7803 1501.7923 -7.98 1 20 0.42 +1Score > 41 indicates identity
Score > 29 indicates homology		 ALEAVCGSLVSQIR
2494 834.4493 1666.8840 1666.8852 -0.67 1 20 0.26 +1Score > 39 indicates identity
Score > 26 indicates homology		 VKLLDTMVDTFLQK + Deamidated (NQ); Oxidation (M)
630 408.2345 814.4544 814.4548 -0.49 1 20 0.68 +1Score > 43 indicates identity
Score > 31 indicates homology		 EIIIDGR
1066 499.2936 996.5726 996.5716 1.06 1 20 0.63 +1Score > 37 indicates identity
Score > 30 indicates homology		 NIPVRQIR + 2 Deamidated (NQ)
1627 417.9064 1250.6974 1250.7095 -9.70 1 20 0.65 +1Score > 37 indicates identity
Score > 30 indicates homology		 KAGADGITIHLR
2262 782.4316 1562.8486 1562.8417 4.46 1 20 0.68 +1Score > 38 indicates identity
Score > 31 indicates homology		 RQVGVPILTDVHTE
2135 752.3915 1502.7684 1502.7915 -15.4 1 20 0.61 +1Score > 41 indicates identity
Score > 30 indicates homology		 LCEALSTLGVWVR
1476 594.8375 1187.6604 1187.6510 7.98 1 20 0.67 +1Score > 40 indicates identity
Score > 31 indicates homology		 LTKSQLQQNK + Deamidated (NQ)
773 440.2528 878.4910 878.4861 5.60 1 20 0.64 +1Score > 38 indicates identity
Score > 30 indicates homology		 KAAVQYAK + Deamidated (NQ)
426 358.7019 715.3892 715.3864 3.94 0 20 0.56 +1Score > 44 indicates identity
Score > 30 indicates homology		 QDAAALK
2442 545.6286 1633.8640 1633.8536 6.36 1 20 0.48 +1Score > 39 indicates identity
Score > 29 indicates homology		 TGRSAQLLSQYRPR + 2 Deamidated (NQ)
2859 623.3375 1866.9907 1867.0051 -7.70 1 20 0.057 +1Score > 38 indicates identity
Score > 20 indicates homology		 ALDTAAVSAPQLQLAIRE + Deamidated (NQ)
1796 669.8264 1337.6382 1337.6649 -19.9 1 20 0.49 +1Score > 41 indicates identity
Score > 29 indicates homology		 MEFQTTLPSIR + Oxidation (M)
2166 506.2684 1515.7834 1515.7868 -2.24 1 20 0.92 +1Score > 41 indicates identity
Score > 32 indicates homology		 WGTPITMDAAVKAR
276 322.2119 642.4092 642.4177 -13.1 1 20 0.63 +1Score > 41 indicates identity
Score > 30 indicates homology		 KGAVIR
435 360.7249 719.4352 719.4330 3.13 0 20 0.64 +1Score > 36 indicates identity
Score > 30 indicates homology		 YLVVAR
307 332.1832 662.3518 662.3500 2.78 0 20 0.85 +1Score > 41 indicates identity
Score > 31 indicates homology		 FGNVAR
293 652.3083 651.3010 651.3010 0.021 0 20 0.97 +1Score > 40 indicates identity
Score > 32 indicates homology		 SSMGVR + Oxidation (M)
2307 791.3882 1580.7618 1580.7769 -9.55 1 20 0.84 +1Score > 41 indicates identity
Score > 31 indicates homology		 QDIQKLFSTWCR
1071 333.8588 998.5546 998.5621 -7.54 1 19 0.78 +1Score > 39 indicates identity
Score > 31 indicates homology		 TLRAQPSAR
1046 495.2812 988.5478 988.5342 13.8 0 19 0.9 +1Score > 40 indicates identity
Score > 32 indicates homology		 LYGDPLGVR
1480 398.2016 1191.5830 1191.5884 -4.54 0 19 0.65 +1Score > 41 indicates identity
Score > 30 indicates homology		 NPSVASQYGIR + Deamidated (NQ)
1783 444.5917 1330.7533 1330.7344 14.2 1 19 0.13 +1Score > 37 indicates identity
Score > 23 indicates homology		 TTVQIKGDLLDK + Deamidated (NQ)
1983 714.4031 1426.7916 1426.7827 6.30 1 19 0.85 +1Score > 38 indicates identity
Score > 31 indicates homology		 ANANQLRQLLMR
1447 589.8152 1177.6158 1177.6091 5.69 1 19 0.2 +1Score > 41 indicates identity
Score > 25 indicates homology		 RGTAVTFLGQE
1706 432.9160 1295.7262 1295.7193 5.34 1 19 0.61 +1Score > 37 indicates identity
Score > 30 indicates homology		 MSMLSTLLLKK + 2 Oxidation (M)
539 387.7413 773.4680 773.4759 -10.2 1 19 0.89 +1Score > 38 indicates identity
Score > 31 indicates homology		 ISLSAKR
1024 488.7844 975.5542 975.5614 -7.30 1 19 0.64 +1Score > 39 indicates identity
Score > 30 indicates homology		 LYQQRIR
761 874.4254 873.4181 873.4192 -1.22 1 19 0.6 +1Score > 42 indicates identity
Score > 30 indicates homology		 KHLSSSSE
1599 620.3402 1238.6658 1238.6619 3.20 1 19 0.62 +1Score > 39 indicates identity
Score > 30 indicates homology		 AHGQKVADALTK + Deamidated (NQ)
795 444.7471 887.4796 887.4712 9.51 0 19 0.61 +1Score > 42 indicates identity
Score > 30 indicates homology		 NLQNSLAK + Deamidated (NQ)
1580 411.8964 1232.6674 1232.6652 1.74 0 19 0.44 +1Score > 40 indicates identity
Score > 28 indicates homology		 TLNLLFALAQE + Deamidated (NQ)
1594 619.8713 1237.7280 1237.7143 11.1 1 19 0.34 +1Score > 34 indicates identity
Score > 27 indicates homology		 VDKPVRSVNPK
1415 583.3291 1164.6436 1164.6292 12.4 0 19 0.75 +1Score > 38 indicates identity
Score > 31 indicates homology		 TLTQFTWLR
2743 599.3311 1794.9715 1794.9727 -0.67 0 19 0.36 +1Score > 37 indicates identity
Score > 27 indicates homology		 IQNIVASANLGKPLNLE + 2 Deamidated (NQ)
842 453.7691 905.5236 905.5083 17.0 1 19 0.8 +1Score > 38 indicates identity
Score > 31 indicates homology		 IRLAYNR + Deamidated (NQ)
1506 602.8414 1203.6682 1203.6836 -12.8 1 19 0.87 +1Score > 39 indicates identity
Score > 31 indicates homology		 IIAGHPRIDGR
3007 980.0214 1958.0282 1957.9938 17.6 0 19 0.16 +1Score > 39 indicates identity
Score > 24 indicates homology		 FLSQPFFVAAQFTGVDGK
2512 557.3365 1668.9877 1668.9563 18.8 1 19 0.3 +1Score > 30 indicates identity
Score > 27 indicates homology		 SRLPLLDLNIYVPR + Deamidated (NQ)
368 346.6729 691.3312 691.3249 9.18 0 19 0.39 +1Score > 43 indicates identity
Score > 28 indicates homology		 AGGSSSAR
644 412.7367 823.4588 823.4664 -9.19 0 19 0.97 +1Score > 38 indicates identity
Score > 32 indicates homology		 HLASLAGR
1061 332.8550 995.5432 995.5400 3.19 1 19 0.19 +1Score > 37 indicates identity
Score > 25 indicates homology		 EGQVTPLPR
142 516.2919 515.2846 515.2816 5.93 0 19 0.63 +1Score > 40 indicates identity
Score > 30 indicates homology		 AGAAAR
416 357.2205 712.4264 712.4232 4.62 0 19 0.32 +1Score > 34 indicates identity
Score > 27 indicates homology		 SPIIQR
1274 551.3005 1100.5864 1100.5965 -9.13 1 19 0.34 +1Score > 42 indicates identity
Score > 27 indicates homology		 LVLTDELVAE
3205 696.3411 2086.0015 2085.9816 9.53 1 19 0.22 +1Score > 39 indicates identity
Score > 25 indicates homology		 ALDTYNEINAVMNLVLFE + 2 Deamidated (NQ); Oxidation (M)
3014 656.3425 1966.0057 1965.9717 17.3 1 19 0.51 +1Score > 39 indicates identity
Score > 29 indicates homology		 IPPPAMNEIILGANQDVR + 3 Deamidated (NQ); Oxidation (M)
3141 1020.5797 2039.1448 2039.1415 1.64 1 19 0.15 +1Score > 33 indicates identity
Score > 23 indicates homology		 TYLVTVAPLESALLLSGHR
2647 871.4683 1740.9220 1740.9046 9.99 1 19 0.52 +1Score > 39 indicates identity
Score > 29 indicates homology		 LSDGAPLTVYPGEVPAR
224 308.6874 615.3602 615.3592 1.77 0 19 0.73 +1Score > 45 indicates identity
Score > 30 indicates homology		 ADAIVK
345 340.6808 679.3470 679.3442 4.20 0 19 0.92 +1Score > 40 indicates identity
Score > 31 indicates homology		 FPNFR
390 351.7018 701.3890 701.3894 -0.53 0 19 0.98 +1Score > 42 indicates identity
Score > 31 indicates homology		 VSMLPR
1851 683.9133 1365.8120 1365.7868 18.5 0 19 0.098 +1Score > 32 indicates identity
Score > 21 indicates homology		 GLVSTPAGPVNLLK + Deamidated (NQ)
2015 722.4282 1442.8418 1442.8133 19.8 1 19 0.25 +1Score > 34 indicates identity
Score > 26 indicates homology		 EAIIVTLPPATYR
1600 413.9052 1238.6938 1238.7095 -12.7 1 19 0.43 +1Score > 38 indicates identity
Score > 28 indicates homology		 QKAVVAGRPDAK
1890 694.3928 1386.7710 1386.7718 -0.57 1 19 0.62 +1Score > 38 indicates identity
Score > 29 indicates homology		 VQSIVSEQLGISK
914 468.2759 934.5372 934.5348 2.58 1 19 0.22 +1Score > 37 indicates identity
Score > 25 indicates homology		 GRHPLLDK
3177 1030.5413 2059.0680 2059.0375 14.9 1 19 0.33 +1Score > 38 indicates identity
Score > 27 indicates homology		 GDKVQGDGDWKPIVQFLR + 2 Deamidated (NQ)
1469 593.8387 1185.6628 1185.6717 -7.48 1 19 0.57 +1Score > 40 indicates identity
Score > 29 indicates homology		 AQVDVGSLRLK + Deamidated (NQ)
1629 418.2281 1251.6625 1251.6459 13.2 0 19 0.25 +1Score > 39 indicates identity
Score > 25 indicates homology		 DAVGTYTLSGLR
997 483.7507 965.4868 965.4818 5.23 1 19 0.86 +1Score > 41 indicates identity
Score > 31 indicates homology		 KFQATDQK + Deamidated (NQ)
1953 472.5771 1414.7095 1414.7052 3.02 0 19 0.68 +1Score > 41 indicates identity
Score > 30 indicates homology		 TANAIVSQQTPQR + 2 Deamidated (NQ)
1929 351.6887 1402.7257 1402.7456 -14.2 1 19 0.79 +1Score > 41 indicates identity
Score > 30 indicates homology		 TEATQGLILWSGK
1703 647.9138 1293.8130 1293.8271 -10.9 0 19 0.38 +1Score > 27 indicates identity ILLPAISDVILK
223 308.6752 615.3358 615.3452 -15.2 1 19 0.76 +1Score > 44 indicates identity
Score > 30 indicates homology		 AGNKAR
1895 695.3551 1388.6956 1388.6864 6.67 1 19 0.5 +1Score > 41 indicates identity
Score > 28 indicates homology		 LYFIVKDFDTE
923 470.3015 938.5884 938.5913 -3.03 0 19 0.88 +1Score > 32 indicates identity
Score > 31 indicates homology		 IIIPGVTAR
2118 749.9407 1497.8668 1497.8402 17.8 1 19 0.76 +1Score > 35 indicates identity
Score > 30 indicates homology		 ISIAGRLVADLLQE + Deamidated (NQ)
1494 600.3387 1198.6628 1198.6418 17.5 1 19 0.67 +1Score > 40 indicates identity
Score > 30 indicates homology		 DVLALARDGNR
2017 722.8694 1443.7242 1443.7358 -7.99 1 19 0.54 +1Score > 41 indicates identity
Score > 29 indicates homology		 WDVNIGELAGITR + Deamidated (NQ)
2486 555.9960 1664.9662 1664.9535 7.62 0 19 0.17 +1Score > 32 indicates identity
Score > 24 indicates homology		 SMPPAVLAGQIIDLLK
1620 624.8417 1247.6688 1247.6510 14.3 1 19 0.41 +1Score > 40 indicates identity
Score > 27 indicates homology		 IDKNDLDIFR
1147 1031.5314 1030.5241 1030.5295 -5.18 0 19 0.15 +1Score > 42 indicates identity
Score > 23 indicates homology		 DLLLNTADR + Deamidated (NQ)
3060 664.0175 1989.0307 1989.0241 3.32 1 19 0.099 +1Score > 39 indicates identity
Score > 21 indicates homology		 MSNKGQLLQDPFLNLLR + 3 Deamidated (NQ)
1418 583.8329 1165.6512 1165.6342 14.6 1 19 0.3 +1Score > 37 indicates identity
Score > 26 indicates homology		 NAIGIELPNPK + Deamidated (NQ)
1700 647.3290 1292.6434 1292.6500 -5.04 1 19 0.13 +1Score > 41 indicates identity
Score > 22 indicates homology		 DAILVNVYQEK + 2 Deamidated (NQ)
3773 799.4258 2395.2556 2395.2608 -2.16 1 19 0.62 +1Score > 37 indicates identity
Score > 29 indicates homology		 APNRYLGGAIAIGPQTAADALAQR + Deamidated (NQ)
2386 807.4176 1612.8206 1612.8308 -6.30 1 19 0.72 +1Score > 40 indicates identity
Score > 30 indicates homology		 VTGALESGANAVLVSPE
796 445.7253 889.4360 889.4327 3.74 1 19 0.29 +1Score > 42 indicates identity
Score > 26 indicates homology		 NMDEILR
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