MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
945 316.5041 946.4905 946.5083 -18.9 1 23 0.087 +1Score > 42 indicates identity
Score > 25 indicates homology		 KSSIVDGNK
339 338.1833 674.3520 674.3460 9.00 1 23 0.55 +1Score > 43 indicates identity
Score > 32 indicates homology		 TGRDAR
902 464.7129 927.4112 927.3974 14.9 1 23 0.23 +1Score > 38 indicates identity
Score > 29 indicates homology		 EFFDQNK + Deamidated (NQ)
1441 587.7806 1173.5466 1173.5666 -17.0 1 23 0.97 +1Score > 40 indicates identity
Score > 35 indicates homology		 LQEAWNDAVK + Deamidated (NQ)
1165 521.2919 1040.5692 1040.5727 -3.28 1 22 0.64 +1Score > 39 indicates identity
Score > 33 indicates homology		 GGSIRNAIPR + Deamidated (NQ)
940 473.2498 944.4850 944.4814 3.81 1 22 0.33 +1Score > 43 indicates identity
Score > 30 indicates homology		 LDQLAAEGK + Deamidated (NQ)
958 475.7532 949.4918 949.4869 5.25 1 22 0.49 +1Score > 42 indicates identity
Score > 32 indicates homology		 AYLDEAIR
2359 803.9297 1605.8448 1605.8436 0.77 1 22 0.21 +1Score > 39 indicates identity
Score > 28 indicates homology		 YQLVIEGMVLAGQGK + Deamidated (NQ)
2222 774.3989 1546.7832 1546.8103 -17.5 1 22 0.13 +1Score > 41 indicates identity
Score > 26 indicates homology		 EAAAPGAAPAAAAAAPAAK
1653 635.3812 1268.7478 1268.7704 -17.8 0 22 0.92 +1Score > 36 indicates identity
Score > 35 indicates homology		 LIVIDLLSVQR + Deamidated (NQ)
1465 593.3398 1184.6650 1184.6513 11.6 1 22 0.76 +1Score > 39 indicates identity
Score > 34 indicates homology		 VATAGLKGSPQR + Deamidated (NQ)
2211 771.9213 1541.8280 1541.8123 10.2 1 22 0.35 +1Score > 39 indicates identity
Score > 30 indicates homology		 AAVINNAMLEAILAE
1973 712.3958 1422.7770 1422.7619 10.6 1 22 0.19 +1Score > 38 indicates identity
Score > 28 indicates homology		 LAVDVGYNFLRR + Deamidated (NQ)
518 382.7333 763.4520 763.4480 5.34 0 22 0.57 +1Score > 35 indicates identity
Score > 32 indicates homology		 YIDIIK
966 319.8394 956.4964 956.5113 -15.6 0 22 0.35 +1Score > 40 indicates identity
Score > 30 indicates homology		 LCLPQGLR + Deamidated (NQ)
1317 564.8069 1127.5992 1127.5896 8.53 1 22 0.79 +1Score > 41 indicates identity
Score > 34 indicates homology		 LVEIQMPAVE
1901 695.8877 1389.7608 1389.7616 -0.53 1 22 0.48 +1Score > 39 indicates identity
Score > 32 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
935 472.2476 942.4806 942.4770 3.83 1 22 0.33 +1Score > 39 indicates identity
Score > 30 indicates homology		 NLLEDPSR
1516 606.3444 1210.6742 1210.6822 -6.57 1 22 0.68 +1Score > 38 indicates identity
Score > 33 indicates homology		 QSILLRYYR
1224 359.5336 1075.5790 1075.5873 -7.77 0 22 0.98 +1Score > 41 indicates identity
Score > 35 indicates homology		 LDITSSTAIR
2300 790.4515 1578.8884 1578.8729 9.83 1 22 0.43 +1Score > 35 indicates identity
Score > 31 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
2217 772.4314 1542.8482 1542.8439 2.81 0 22 0.14 +1Score > 38 indicates identity
Score > 26 indicates homology		 NMVVSNILLNNIAK + Deamidated (NQ)
1560 614.8383 1227.6620 1227.6459 13.2 1 22 0.42 +1Score > 40 indicates identity
Score > 31 indicates homology		 QLQLAQEAAQK + Deamidated (NQ)
1736 657.3482 1312.6818 1312.6623 14.9 1 22 0.47 +1Score > 40 indicates identity
Score > 31 indicates homology		 QGLARDGKPIQE + 2 Deamidated (NQ)
2536 559.2834 1674.8284 1674.8134 8.93 1 22 0.14 +1Score > 41 indicates identity
Score > 26 indicates homology		 GENIINIITGPNMSGK + 2 Deamidated (NQ); Oxidation (M)
2329 530.9072 1589.6998 1589.7007 -0.55 0 22 0.11 +1Score > 38 indicates identity
Score > 25 indicates homology		 DHGMYFMYQILR + Deamidated (NQ); Oxidation (M)
1732 437.2227 1308.6463 1308.6561 -7.53 1 22 0.094 +1Score > 41 indicates identity
Score > 24 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
1497 601.3393 1200.6640 1200.6437 16.9 0 22 0.6 +1Score > 40 indicates identity
Score > 32 indicates homology		 MLNVVWNAVR
400 352.2040 702.3934 702.3912 3.21 0 22 0.84 +1Score > 44 indicates identity
Score > 34 indicates homology		 AGIDLSK
1806 448.6022 1342.7848 1342.7820 2.07 1 22 0.74 +1Score > 35 indicates identity
Score > 33 indicates homology		 TQLGKLVASGQIK + Deamidated (NQ)
1748 660.3511 1318.6876 1318.6656 16.7 1 22 0.83 +1Score > 41 indicates identity
Score > 34 indicates homology		 FLDEGVVVAQLE + Deamidated (NQ)
534 386.7153 771.4160 771.4239 -10.2 0 22 0.4 +1Score > 41 indicates identity
Score > 30 indicates homology		 NVVDLGR
1242 543.3159 1084.6172 1084.6101 6.57 1 22 0.96 +1Score > 39 indicates identity
Score > 34 indicates homology		 AVITRNGQAR
1688 644.8524 1287.6902 1287.6922 -1.49 0 22 0.18 +1Score > 41 indicates identity
Score > 27 indicates homology		 TVSLQALQQLGK + 3 Deamidated (NQ)
752 435.2459 868.4772 868.4694 8.99 0 22 0.13 +1Score > 37 indicates identity
Score > 26 indicates homology		 IYQFIGK + Deamidated (NQ)
1952 707.9135 1413.8124 1413.8132 -0.55 1 22 1 +1Score > 35 indicates identity
Score > 34 indicates homology		 LLFLIPPEHAHK
1381 575.7946 1149.5746 1149.5554 16.8 1 22 0.85 +1Score > 42 indicates identity
Score > 34 indicates homology		 TVIDFDPKSE
1905 695.8904 1389.7662 1389.7616 3.36 1 22 0.23 +1Score > 38 indicates identity
Score > 28 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
301 329.7115 657.4084 657.4061 3.55 1 22 0.9 +1Score > 41 indicates identity
Score > 34 indicates homology		 AVEVIK
406 352.6949 703.3752 703.3752 0.071 1 22 0.72 +1Score > 44 indicates identity
Score > 33 indicates homology		 VEQISK + Deamidated (NQ)
1861 686.3683 1370.7220 1370.7405 -13.5 1 22 0.44 +1Score > 40 indicates identity
Score > 31 indicates homology		 VLSQQAAAVVKQE + Deamidated (NQ)
1809 672.8514 1343.6882 1343.6681 15.0 0 22 0.83 +1Score > 41 indicates identity
Score > 33 indicates homology		 QDVSLALDVDNR
540 388.1920 774.3694 774.3620 9.62 1 22 0.77 +1Score > 42 indicates identity
Score > 33 indicates homology		 GGQNKNR + 2 Deamidated (NQ)
510 381.2077 760.4008 760.4153 -19.0 0 22 0.34 +1Score > 44 indicates identity
Score > 29 indicates homology		 AMVQAIK + Deamidated (NQ)
951 474.7656 947.5166 947.5110 5.96 0 22 0.7 +1Score > 40 indicates identity
Score > 33 indicates homology		 MGILSVQGK + Oxidation (M)
1144 1031.5146 1030.5073 1030.4931 13.8 0 22 0.83 +1Score > 41 indicates identity
Score > 33 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
1714 650.8611 1299.7076 1299.7034 3.25 0 22 0.06 +1Score > 40 indicates identity
Score > 22 indicates homology		 VLDIAGGTGDLAAK
2198 768.4128 1534.8110 1534.7813 19.4 1 22 0.12 +1Score > 40 indicates identity
Score > 25 indicates homology		 AQPIYIRTPSTCK + Deamidated (NQ)
1468 593.7999 1185.5852 1185.6063 -17.8 1 22 0.95 +1Score > 40 indicates identity
Score > 34 indicates homology		 QPTMPILKNE + Oxidation (M)
747 434.2584 866.5022 866.4974 5.62 1 22 0.34 +1Score > 36 indicates identity
Score > 29 indicates homology		 HEILISR
1411 388.2091 1161.6055 1161.5998 4.86 0 22 0.49 +1Score > 41 indicates identity
Score > 31 indicates homology		 MGVAMGILASGR
1604 414.2465 1239.7177 1239.7339 -13.1 1 22 0.13 +1Score > 37 indicates identity
Score > 25 indicates homology		 HPSKLLAYALK
1725 653.8407 1305.6668 1305.6758 -6.82 1 22 0.14 +1Score > 42 indicates identity
Score > 25 indicates homology		 AFFKGVQAYFK + Deamidated (NQ)
1478 595.3405 1188.6664 1188.6615 4.16 0 22 0.093 +1Score > 39 indicates identity
Score > 24 indicates homology		 TVHPLADLAPR
1253 544.3108 1086.6070 1086.6073 -0.26 0 21 0.22 +1Score > 41 indicates identity
Score > 27 indicates homology		 VPIQIQNFK + Deamidated (NQ)
453 365.2228 728.4310 728.4181 17.8 0 21 0.76 +1Score > 44 indicates identity
Score > 33 indicates homology		 QDVIVR
1751 660.8284 1319.6422 1319.6622 -15.1 1 21 0.43 +1Score > 41 indicates identity
Score > 30 indicates homology		 INQQAFQWKR + 2 Deamidated (NQ)
1770 663.4196 1324.8246 1324.8078 12.7 1 21 0.096 +1Score > 28 indicates identity
Score > 24 indicates homology		 NVVVVAANGKILK + Deamidated (NQ)
925 470.7919 939.5692 939.5753 -6.44 0 21 0.77 +1Score > 35 indicates identity
Score > 33 indicates homology		 QPVLLITR + Deamidated (NQ)
218 614.4007 613.3934 613.3911 3.72 0 21 0.42 +1Score > 40 indicates identity
Score > 30 indicates homology		 AGVVLR
938 472.7946 943.5746 943.5702 4.69 1 21 0.32 +1Score > 36 indicates identity
Score > 29 indicates homology		 TITVLIER
1518 606.3459 1210.6772 1210.6822 -4.09 1 21 0.93 +1Score > 38 indicates identity
Score > 33 indicates homology		 QSILLRYYR
2726 893.9806 1785.9466 1785.9585 -6.63 1 21 0.33 +1Score > 39 indicates identity
Score > 29 indicates homology		 RIQGGAADTVLLGATGTGK + Deamidated (NQ)
3118 1017.5396 2033.0646 2033.0252 19.4 1 21 0.31 +1Score > 38 indicates identity
Score > 29 indicates homology		 NQVSFLLNTGVMQPSEIR + Deamidated (NQ)
897 464.2745 926.5344 926.5371 -2.89 0 21 0.66 +1Score > 38 indicates identity
Score > 32 indicates homology		 NLLMALPR
1540 610.3040 1218.5934 1218.6139 -16.8 1 21 0.99 +1Score > 42 indicates identity
Score > 34 indicates homology		 ASQTLCNIRR + Deamidated (NQ)
3734 788.7218 2363.1436 2363.1129 13.0 1 21 0.16 +1Score > 39 indicates identity
Score > 26 indicates homology		 TVAGPGEGTVAPTALQGPSPGSPGQE + 2 Deamidated (NQ)
2917 633.6572 1897.9498 1897.9758 -13.7 1 21 0.083 +1Score > 39 indicates identity
Score > 23 indicates homology		 ARPGSFDRPLAENNVVR + Deamidated (NQ)
1863 686.3721 1370.7296 1370.7518 -16.1 1 21 0.28 +1Score > 40 indicates identity
Score > 28 indicates homology		 LSDDVIDLIGRR
3165 1029.5303 2057.0460 2057.0211 12.1 1 21 0.29 +1Score > 39 indicates identity
Score > 28 indicates homology		 ALANAEVNGQAGGGLVQVTMR + 2 Deamidated (NQ)
467 367.6859 733.3572 733.3606 -4.61 1 21 0.49 +1Score > 43 indicates identity
Score > 30 indicates homology		 SQVSGKE
990 481.7915 961.5684 961.5596 9.15 0 21 0.12 +1Score > 36 indicates identity
Score > 24 indicates homology		 SLINFAIGK
2742 897.9694 1793.9242 1793.9523 -15.6 1 21 0.28 +1Score > 39 indicates identity
Score > 28 indicates homology		 QQDIPVENVLALGGIAR + 2 Deamidated (NQ)
1841 679.8697 1357.7248 1357.6990 19.0 1 21 0.46 +1Score > 40 indicates identity
Score > 30 indicates homology		 NVITWQELQAR + Deamidated (NQ)
2213 772.4093 1542.8040 1542.8140 -6.48 0 21 0.078 +1Score > 40 indicates identity
Score > 22 indicates homology		 LAIQNSQIASAIALE + 2 Deamidated (NQ)
530 385.7303 769.4460 769.4446 1.85 0 21 0.7 +1Score > 37 indicates identity
Score > 32 indicates homology		 LPNGTIR
707 854.4761 853.4688 853.4657 3.63 0 21 0.81 +1Score > 38 indicates identity
Score > 32 indicates homology		 APAAAPAASK
281 644.4129 643.4056 643.4017 6.12 1 21 0.72 +1Score > 42 indicates identity
Score > 32 indicates homology		 AGGKLAK
3068 999.5327 1997.0508 1997.0331 8.91 0 21 0.14 +1Score > 38 indicates identity
Score > 25 indicates homology		 LVIDAGNTNIVFAVHDGSR
905 310.5133 928.5181 928.5130 5.43 1 21 0.91 +1Score > 41 indicates identity
Score > 33 indicates homology		 IYEHVLR
2584 854.9546 1707.8946 1707.8679 15.7 1 21 0.51 +1Score > 39 indicates identity
Score > 30 indicates homology		 QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
981 480.7735 959.5324 959.5287 3.87 1 21 0.47 +1Score > 41 indicates identity
Score > 30 indicates homology		 LGNNVKVSK + 2 Deamidated (NQ)
500 380.2223 758.4300 758.4286 1.84 0 21 0.81 +1Score > 44 indicates identity
Score > 32 indicates homology		 AVGAVSAGK
1965 474.5822 1420.7248 1420.7166 5.72 0 21 0.23 +1Score > 41 indicates identity
Score > 27 indicates homology		 LGLSNMQGMSVLR + Oxidation (M)
883 461.7369 921.4592 921.4630 -4.04 0 21 0.91 +1Score > 42 indicates identity
Score > 33 indicates homology		 IWQIMSK + Deamidated (NQ); Oxidation (M)
971 319.8680 956.5822 956.5728 9.77 1 21 0.4 +1Score > 38 indicates identity
Score > 29 indicates homology		 KMLPNLLK + Deamidated (NQ)
193 601.3333 600.3260 600.3344 -13.9 0 21 0.88 +1Score > 39 indicates identity
Score > 33 indicates homology		 QVGAAR
564 392.7569 783.4992 783.4967 3.31 1 21 0.39 +1Score > 36 indicates identity
Score > 29 indicates homology		 VAKLPTR
202 602.3646 601.3573 601.3548 4.28 0 21 0.59 +1Score > 43 indicates identity
Score > 31 indicates homology		 GVASLR
2815 614.0070 1838.9992 1838.9778 11.6 0 21 0.2 +1Score > 37 indicates identity
Score > 26 indicates homology		 GSVTNVLTSIVGNVFGFK + Deamidated (NQ)
1190 353.5281 1057.5625 1057.5768 -13.5 1 21 0.24 +1Score > 41 indicates identity
Score > 27 indicates homology		 NTVDVEILR
726 429.7700 857.5254 857.5222 3.77 0 21 0.38 +1Score > 37 indicates identity
Score > 29 indicates homology		 TQVIAVVK + Deamidated (NQ)
1924 701.3979 1400.7812 1400.7664 10.6 1 20 0.28 +1Score > 38 indicates identity
Score > 27 indicates homology		 DVGWTANKIVLGK + Deamidated (NQ)
1045 330.5140 988.5202 988.5124 7.86 1 20 0.68 +1Score > 42 indicates identity
Score > 31 indicates homology		 SCPVKGSVR
2049 731.9271 1461.8396 1461.8303 6.38 1 20 0.84 +1Score > 34 indicates identity
Score > 32 indicates homology		 SYRQQINSILLK
2298 790.4496 1578.8846 1578.8729 7.42 1 20 0.25 +1Score > 36 indicates identity
Score > 27 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
2623 576.9521 1727.8345 1727.8665 -18.5 1 20 0.14 +1Score > 40 indicates identity
Score > 24 indicates homology		 HAQLPLADFVKMNSR + 2 Deamidated (NQ)
1150 345.5030 1033.4872 1033.4862 0.90 1 20 0.63 +1Score > 41 indicates identity
Score > 31 indicates homology		 IDAMGGEIGR + Oxidation (M)
1949 707.3741 1412.7336 1412.7147 13.4 0 20 0.96 +1Score > 40 indicates identity
Score > 33 indicates homology		 NAVVLLNTQGEPR + 3 Deamidated (NQ)
1881 462.9204 1385.7394 1385.7337 4.12 1 20 0.41 +1Score > 40 indicates identity
Score > 29 indicates homology		 RVAIASVLAMNPE + Oxidation (M)
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