MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4839 1022.3130 4085.2229
4840 1022.3161 4085.2353
4841 1022.5734 4086.2645
4842 684.3518 4100.0671
4844 1027.0385 4104.1249
4845 1027.2832 4105.1037
4847 1027.5354 4106.1125
4848 1031.3254 4121.2725
4849 1033.0370 4128.1189
4851 1035.7687 4139.0457
4852 1041.0521 4160.1793
4853 1043.8091 4171.2073
4854 1045.3177 4177.2417
4856 1046.8403 4183.3321
4860 1051.2921 4201.1393
4861 1052.5455 4206.1529
4863 1074.0444 4292.1485
4864 1079.8163 4315.2361
4866 865.2504 4321.2156
4867 1085.5704 4338.2525
4868 868.8584 4339.2556
4869 1091.3462 4361.3557
4871 1094.3168 4373.2381
4873 1098.5859 4390.3145
4874 1099.8036 4395.1853
4875 1104.5627 4414.2217
4876 885.4376 4422.1516
4878 1108.0903 4428.3321
4879 887.4482 4432.2046
4881 1111.6353 4442.5121
4883 1117.5841 4466.3073
4884 1120.1149 4476.4305
4886 897.8821 4484.3741
4888 899.0533 4490.2301
4891 902.4943 4507.4351
4892 904.8828 4519.3776
4893 1138.6090 4550.4069
4894 1145.6355 4578.5129
4895 917.5117 4582.5221
4896 1149.6003 4594.3721
4897 920.4669 4597.2981
4898 920.4761 4597.3441
4899 661.6166 4624.2653
4900 771.7192 4624.2715
4904 1160.5787 4638.2857
4905 928.6810 4638.3686
4906 1163.1394 4648.5285
4907 930.7145 4648.5361
4908 776.7319 4654.3477
4909 1165.0719 4656.2585
4910 777.4080 4658.4043
4912 940.0838 4695.3826
4914 1175.8574 4699.4005
4915 1185.8795 4739.4889
4919 803.5784 4815.4267
4920 808.4563 4844.6941
4921 809.2987 4849.7485
4923 979.7130 4893.5286
4925 985.5153 4922.5401
4926 985.9315 4924.6211
4927 990.5260 4947.5936
4929 1004.5209 5017.5681
4930 1009.4914 5042.4206
4932 1021.5693 5102.8101
4933 1021.5731 5102.8291
4934 744.9842 5207.8385
4935 869.1486 5208.8479
4937 1050.9249 5249.5881
4938 1052.7837 5258.8821
4940 877.9493 5261.6521
4941 1053.5289 5262.6081
4942 1053.5320 5262.6236
4943 878.4944 5264.9227
4944 886.4411 5312.6029
4945 1063.7329 5313.6281
4947 1067.1388 5330.6576
4948 904.8018 5422.7671
4950 908.4312 5444.5435
4951 917.6022 5499.5695
4952 1106.5604 5527.7656
4953 929.4442 5570.6215
4954 1116.4415 5577.1711
4955 1116.6490 5578.2086
4956 704.2584 5626.0090
4957 1145.2161 5721.0441
4958 1149.0276 5740.1016
4960 1156.6211 5778.0691
4961 1156.6229 5778.0781
4963 973.0370 5832.1783
4964 1169.6134 5843.0306
4965 1183.3854 5911.8906
4966 986.4847 5912.8645
4967 1183.5820 5912.8736
4968 993.4892 5954.8915
4971 1049.8882 6293.2855
4972 1058.5244 6345.1027
4973 908.1955 6350.3176
4974 1059.5514 6351.2647
4975 908.3381 6351.3158
4976 1059.5647 6351.3445
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