MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4697 903.2278 3608.8821
4698 903.4818 3609.8981
4699 905.6473 3618.5601
4700 724.9209 3619.5681
4701 905.9531 3619.7833
4703 906.7484 3622.9645
4706 907.4678 3625.8421
4707 909.4603 3633.8121
4708 910.7745 3639.0689
4711 914.7242 3654.8677
4712 914.9767 3655.8777
4714 916.9966 3663.9573
4716 919.0134 3672.0245
4717 736.6014 3677.9706
4718 920.7534 3678.9845
4719 921.0023 3679.9801
4721 921.4725 3681.8609
4722 737.3807 3681.8671
4723 921.5123 3682.0201
4725 738.3441 3686.6841
4727 529.7143 3700.9492
4728 926.4616 3701.8173
4729 529.8577 3701.9530
4731 741.6173 3703.0501
4733 743.5870 3712.8986
4734 930.5015 3717.9769
4735 932.0163 3724.0361
4736 932.5012 3725.9757
4738 747.4230 3732.0786
4740 936.5195 3742.0489
4742 937.5139 3746.0265
4743 938.7733 3751.0641
4744 940.0122 3756.0197
4747 943.5054 3769.9925
4748 946.5057 3781.9937
4749 757.4064 3781.9956
4750 757.6067 3782.9971
4752 947.5252 3786.0717
4753 949.4983 3793.9641
4754 951.5020 3801.9789
4756 761.4175 3802.0511
4757 761.4191 3802.0591
4758 952.7220 3806.8589
4760 953.1911 3808.7353
4761 765.4341 3822.1341
4762 957.5142 3826.0277
4763 766.3797 3826.8621
4765 960.5267 3838.0777
4766 768.6238 3838.0826
4767 768.8094 3839.0106
4769 770.8225 3849.0761
4770 771.0232 3850.0796
4772 963.5302 3850.0917
4773 963.5308 3850.0941
4774 967.5458 3866.1541
4775 967.5565 3866.1969
4777 777.4019 3881.9731
4778 973.5062 3889.9957
4779 973.5070 3889.9989
4780 780.2285 3896.1061
4781 975.5283 3898.0841
4783 781.4136 3902.0316
4786 782.4275 3907.1011
4787 977.7884 3907.1245
4789 980.2819 3917.0985
4790 982.5220 3926.0589
4791 989.7891 3955.1273
4792 990.2965 3957.1569
4794 990.7909 3959.1345
4796 1002.0123 4004.0201
4798 1002.2623 4005.0201
4799 1002.2860 4005.1149
4800 1002.2869 4005.1185
4801 1002.3077 4005.2017
4802 802.2365 4006.1461
4803 1002.5661 4006.2353
4804 1003.7462 4010.9557
4806 1003.9968 4011.9581
4807 803.3995 4011.9611
4809 804.4410 4017.1686
4810 1006.2799 4021.0905
4811 1006.2945 4021.1489
4812 1008.0634 4028.2245
4814 810.4244 4047.0856
4815 810.4248 4047.0876
4817 1013.0280 4048.0829
4819 1013.0290 4048.0869
4820 1013.0294 4048.0885
4822 1013.0302 4048.0917
4824 1013.0309 4048.0945
4825 1013.0321 4048.0993
4827 1016.5172 4062.0397
4828 1016.5261 4062.0753
4829 1016.5419 4062.1385
4830 1016.5430 4062.1429
4832 1016.7837 4063.1057
4834 1018.2521 4068.9793
4835 814.8048 4068.9876
4837 1020.5327 4078.1017
4838 818.0469 4085.1981
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