MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4511 801.4594 3201.8085
4512 801.7036 3202.7853
4513 641.5646 3202.7866
4514 801.9598 3203.8101
4515 802.1755 3204.6729
4516 802.4197 3205.6497
4517 1070.5551 3208.6435
4519 1070.8890 3209.6452
4521 803.9271 3211.6793
4524 1074.8845 3221.6317
4528 808.9438 3231.7461
4531 1078.5883 3232.7431
4532 1079.2460 3234.7162
4535 1079.8972 3236.6698
4537 810.7004 3238.7725
4539 1084.2726 3249.7960
4543 813.4610 3249.8149
4544 813.7092 3250.8077
4546 1084.9413 3251.8021
4547 1085.5646 3253.6720
4548 1085.6030 3253.7872
4549 651.9630 3254.7786
4550 815.4371 3257.7193
4551 652.5518 3257.7226
4552 1088.5435 3262.6087
4554 817.4594 3265.8085
4556 1092.5780 3274.7122
4561 657.3507 3281.7171
4562 822.1646 3284.6293
4563 822.3925 3285.5409
4564 1097.9412 3290.8018
4566 1100.1190 3297.3352
4567 1100.5736 3298.6990
4569 826.4180 3301.6429
4573 1103.2759 3306.8059
4575 827.7141 3306.8273
4577 827.9605 3307.8129
4581 827.9628 3307.8221
4582 829.2057 3312.7937
4583 1105.5718 3313.6936
4584 1106.8981 3317.6725
4587 833.1853 3328.7121
4588 833.6865 3330.7169
4592 1112.6016 3334.7830
4593 1113.5985 3337.7737
4599 669.3644 3341.7856
4602 837.9772 3347.8797
4603 837.9789 3347.8865
4605 840.2199 3356.8505
4606 1120.6163 3358.8271
4609 673.7736 3363.8316
4610 842.4638 3365.8261
4611 1124.5989 3370.7749
4613 1125.2532 3372.7378
4615 1125.6154 3373.8244
4616 844.4636 3373.8253
4620 678.5651 3387.7891
4621 678.5653 3387.7901
4623 1130.9285 3389.7637
4627 1133.9000 3398.6782
4631 682.1851 3405.8891
4633 683.5916 3412.9216
4634 854.2408 3412.9341
4635 854.4469 3413.7585
4636 854.4579 3413.8025
4637 855.9816 3419.8973
4638 1142.6249 3424.8529
4641 858.4453 3429.7521
4644 858.7181 3430.8433
4649 861.7361 3442.9153
4650 1149.5785 3445.7137
4652 862.4714 3445.8565
4653 690.3773 3446.8501
4655 1156.9686 3467.8840
4661 873.4819 3489.8985
4663 1164.6350 3490.8832
4664 873.9586 3491.8053
4665 1168.2644 3501.7714
4666 1169.2726 3504.7960
4667 877.6765 3506.6769
4668 878.0040 3507.9869
4669 1173.3112 3516.9118
4670 882.4911 3525.9353
4671 882.4924 3525.9405
4672 883.9833 3531.9041
4673 1178.6409 3532.9009
4675 886.9453 3543.7521
4677 886.9809 3543.8945
4678 889.2783 3553.0841
4680 1185.6180 3553.8322
4683 891.6445 3562.5489
4684 893.4975 3569.9609
4685 715.2015 3570.9711
4686 715.2029 3570.9781
4687 1191.9650 3572.8732
4691 894.5005 3573.9729
4692 894.7454 3574.9525
4693 718.8021 3588.9741
4694 721.3634 3601.7806
4695 901.9557 3603.7937
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