MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4258 722.4073 2885.6001
4259 963.2147 2886.6223
4260 963.4869 2887.4389
4261 963.4944 2887.4614
4263 963.5546 2887.6420
4264 723.6351 2890.5113
4266 724.1224 2892.4605
4269 965.2271 2892.6595
4270 724.3711 2893.4553
4275 966.4843 2896.4311
4276 967.5045 2899.4917
4278 967.8742 2900.6008
4279 967.8780 2900.6122
4280 726.1613 2900.6161
4281 726.4114 2901.6165
4288 728.1315 2908.4969
4289 970.5071 2908.4995
4294 728.4058 2909.5941
4296 971.8425 2912.5057
4299 974.2031 2919.5875
4301 974.5159 2920.5259
4302 975.5301 2923.5685
4305 976.5294 2926.5664
4311 978.8505 2933.5297
4312 734.3914 2933.5365
4314 736.4068 2941.5981
4315 981.8105 2942.4097
4317 984.5292 2950.5658
4329 742.3840 2965.5069
4330 990.5206 2968.5400
4331 991.1853 2970.5341
4332 991.2377 2970.6913
4337 992.8715 2975.5927
4339 746.6907 2982.7337
4342 995.5208 2983.5406
4344 996.5577 2986.6513
4347 747.9176 2987.6413
4350 748.4060 2989.5949
4355 998.5007 2992.4803
4360 1004.1921 3009.5545
4362 1005.9065 3014.6977
4363 1006.2349 3015.6829
4366 1007.5533 3019.6381
4368 1008.1245 3021.3517
4375 757.6469 3026.5585
4376 757.9296 3027.6893
4378 1010.5620 3028.6642
4379 1010.5635 3028.6687
4381 607.5123 3032.5251
4385 1013.5212 3037.5418
4386 760.9067 3039.5977
4389 1016.9012 3047.6818
4398 1018.8341 3053.4805
4399 1018.8388 3053.4946
4403 1021.5312 3061.5718
4405 766.6823 3062.7001
4406 1023.4712 3067.3918
4407 767.8561 3067.3953
4410 1024.2354 3069.6844
4411 770.8915 3079.5369
4412 770.8918 3079.5381
4413 1027.5212 3079.5418
4414 770.8932 3079.5437
4415 1027.5284 3079.5634
4417 1027.5323 3079.5751
4421 771.4482 3081.7637
4424 1028.5543 3082.6411
4429 1031.2292 3090.6658
4431 773.9162 3091.6357
4438 1034.5796 3100.7170
4440 1036.5073 3106.5001
4441 622.9418 3109.6726
4443 779.1750 3112.6709
4444 1038.5721 3112.6945
4447 1040.8871 3119.6395
4448 780.9241 3119.6673
4449 781.1517 3120.5777
4454 1043.5934 3127.7584
4455 1043.9324 3128.7754
4456 783.2020 3128.7789
4458 1044.5555 3130.6447
4459 784.1765 3132.6769
4466 785.1468 3136.5581
4468 1046.5662 3136.6768
4472 1047.2253 3138.6541
4473 1048.1782 3141.5128
4474 787.6754 3146.6725
4475 1050.2281 3147.6625
4476 1050.5656 3148.6750
4477 1051.5494 3151.6264
4478 1053.5625 3157.6657
4479 790.4256 3157.6733
4481 790.4313 3157.6961
4485 792.4297 3165.6897
4492 795.4150 3177.6309
4497 1061.5240 3181.5502
4501 1061.5352 3181.5838
4503 1061.5732 3181.6978
4508 640.1400 3195.6636
4510 641.3636 3201.7816
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