MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3963 645.3331 2577.3033
3964 645.3343 2577.3081
3967 645.8101 2579.2113
3971 861.1504 2580.4294
3976 862.7726 2585.2960
3977 647.3411 2585.3353
3979 863.4495 2587.3267
3980 863.7614 2588.2624
3984 866.7797 2597.3173
3986 650.3496 2597.3693
3987 650.3497 2597.3697
3988 651.1194 2600.4485
3991 870.1495 2607.4267
3992 870.4686 2608.3840
3995 871.4462 2611.3168
3996 873.0883 2616.2431
4008 877.8593 2630.5561
4018 879.4173 2635.2301
4020 880.4651 2638.3735
4023 882.7987 2645.3743
4024 662.5940 2646.3469
4026 883.1489 2646.4249
4029 663.3641 2649.4273
4032 887.1840 2658.5302
4046 892.4519 2674.3339
4050 893.4879 2677.4419
4052 895.1936 2682.5590
4054 895.7527 2684.2363
4062 898.4257 2692.2553
4069 899.4930 2695.4572
4070 900.1512 2697.4318
4071 900.1520 2697.4342
4072 900.1528 2697.4366
4075 901.4619 2701.3639
4078 676.6392 2702.5277
4083 902.8219 2705.4439
4084 903.0743 2706.2011
4088 903.5110 2707.5112
4091 680.9022 2719.5797
4092 907.5381 2719.5925
4095 907.8633 2720.5681
4096 908.0789 2721.2149
4097 908.4669 2722.3789
4100 910.8601 2729.5585
4101 910.8602 2729.5588
4102 683.3986 2729.5653
4106 684.1182 2732.4437
4109 913.1721 2736.4945
4110 913.5057 2737.4953
4112 913.5106 2737.5100
4115 917.8489 2750.5249
4116 917.8494 2750.5264
4117 917.8503 2750.5291
4118 917.8506 2750.5300
4119 688.8896 2751.5293
4130 920.1790 2757.5152
4133 922.4676 2764.3810
4135 922.8359 2765.4859
4137 924.4783 2770.4131
4138 924.4802 2770.4188
4139 555.0917 2770.4221
4140 693.6132 2770.4237
4146 925.5145 2773.5217
4151 926.1739 2775.4999
4152 926.1795 2775.5167
4154 927.4950 2779.4632
4158 929.8693 2786.5861
4161 930.5142 2788.5208
4162 930.7900 2789.3482
4169 698.8890 2791.5269
4170 931.5206 2791.5400
4171 931.5229 2791.5469
4172 931.5347 2791.5823
4175 699.8918 2795.5381
4177 933.8540 2798.5402
4180 934.8439 2801.5099
4182 936.8484 2807.5234
4195 937.5174 2809.5304
4203 941.1402 2820.3988
4209 945.5084 2833.5034
4213 946.5085 2836.5037
4215 948.5016 2842.4830
4218 948.5566 2842.6480
4219 948.8669 2843.5789
4220 949.4858 2845.4356
4223 950.8633 2849.5681
4226 951.5602 2851.6588
4229 955.2172 2862.6298
4230 955.4670 2863.3792
4232 955.5244 2863.5514
4233 955.8537 2864.5393
4234 955.8843 2864.6311
4236 956.1631 2865.4675
4237 956.2122 2865.6148
4240 958.5443 2872.6111
4241 958.5490 2872.6252
4242 719.1639 2872.6265
4244 959.4919 2875.4539
4246 959.5086 2875.5040
4252 721.1530 2880.5829
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