MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3453 1098.5835 2195.1524
3476 736.0972 2205.2698
3482 553.0540 2208.1869
3488 553.8219 2211.2585
3493 739.7571 2216.2495
3503 743.0632 2226.1678
3506 558.3043 2229.1881
3508 744.3419 2230.0039
3510 744.3726 2230.0960
3512 744.3886 2230.1440
3517 744.7340 2231.1802
3518 744.7438 2231.2096
3528 746.7248 2237.1526
3532 746.7275 2237.1607
3533 1121.6239 2241.2332
3538 748.4183 2242.2331
3539 748.4184 2242.2334
3540 561.8083 2243.2041
3542 1123.0421 2244.0696
3543 562.0580 2244.2029
3547 562.5712 2246.2557
3552 1124.6407 2247.2668
3556 751.4088 2251.2046
3558 751.4332 2251.2778
3568 754.3955 2260.1647
3602 761.0707 2280.1903
3608 762.7808 2285.3206
3615 765.4262 2293.2568
3629 768.0373 2301.0901
3643 1154.0958 2306.1770
3644 769.7362 2306.1868
3648 770.4402 2308.2988
3651 770.7752 2309.3038
3658 771.4224 2311.2454
3660 463.6542 2313.2346
3663 579.5688 2314.2461
3672 581.8145 2323.2289
3700 1173.6912 2345.3678
3701 1173.6925 2345.3704
3702 782.7982 2345.3728
3703 782.7994 2345.3764
3704 782.8005 2345.3797
3712 785.4334 2353.2784
3720 787.4216 2359.2430
3729 591.3206 2361.2533
3733 788.4253 2362.2541
3736 789.0993 2364.2761
3749 793.7975 2378.3707
3756 595.8491 2379.3673
3758 795.4445 2383.3117
3765 1195.2101 2388.4056
3768 797.7458 2390.2156
3769 798.0953 2391.2641
3779 801.1211 2400.3415
3786 805.1298 2412.3676
3787 805.1314 2412.3724
3790 604.3378 2413.3221
3791 805.8040 2414.3902
3792 604.8377 2415.3217 2415.2879 14.0 1     +0Score > 34 indicates identity
Score > 13 indicates homology		 SLHPHVPGVTNLQVMRAMTSLK
3793 806.1147 2415.3223
3796 605.0905 2416.3329
3805 808.4588 2422.3546
3807 808.4596 2422.3570
3809 808.4601 2422.3585
3810 808.4604 2422.3594
3811 808.4636 2422.3690
3812 808.4648 2422.3726
3813 808.4672 2422.3798
3815 809.7530 2426.2372
3827 611.2944 2441.1485
3828 611.3713 2441.4561
3834 817.8245 2450.4517
3839 818.4304 2452.2694
3854 823.1404 2466.3994
3855 823.4333 2467.2781
3859 618.6129 2470.4225
3865 826.4381 2476.2925
3870 827.4648 2479.3726
3871 622.8300 2487.2909
3872 830.1450 2487.4132
3873 623.1079 2488.4025
3881 832.4354 2494.2844
3882 832.4365 2494.2877
3897 628.0839 2508.3065
3899 837.3962 2509.1668
3901 628.3346 2509.3093
3902 837.4682 2509.3828
3906 837.8213 2510.4421
3910 839.1444 2514.4114
3925 635.1127 2536.4217
3931 849.8065 2546.3977
3932 849.8323 2546.4751
3937 851.1312 2550.3718
3938 851.4570 2551.3492
3942 852.1204 2553.3394
3944 639.5919 2554.3385
3946 639.6099 2554.4105
3950 854.8776 2561.6110
3954 641.8579 2563.4025
3956 641.8594 2563.4085
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