MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
186 591.8443 590.8370
189 599.6760 598.6687
190 600.0099 599.0026
205 603.0080 602.0007
233 618.8084 617.8011
252 629.8699 628.8626
283 645.8595 644.8522
292 651.3562 650.3489
352 683.8627 682.8554
399 702.7042 701.6969
487 747.9159 746.9086
520 766.1788 765.1715
522 767.4081 766.4008
533 772.4229 771.4156
557 779.9108 778.9035
570 394.4912 786.9678
618 806.4417 805.4344
621 810.4219 809.4146
635 820.4231 819.4158
668 837.1243 836.1170
713 855.0734 854.0661
757 872.4393 871.4320
844 908.4737 907.4664
851 911.4999 910.4926
862 916.4763 915.4690
874 920.4879 919.4806
972 958.1993 957.1920
1000 970.4863 969.4790
1030 978.8438 977.8365
1110 508.3119 1014.6092
1143 1031.1942 1030.1869
1209 1068.2753 1067.2680
1288 557.3688 1112.7230
1322 377.2526 1128.7360
1680 640.8893 1279.7640
1696 430.9364 1289.7874
1877 693.1025 1384.1904
1967 711.8268 1421.6390
1976 713.1518 1424.2890
2079 738.9617 1475.9088
2195 767.4586 1532.9026
2250 520.3151 1557.9235
2286 394.2346 1572.9093
2292 787.9854 1573.9562
2362 804.5013 1606.9880
2367 805.4772 1608.9398
2368 805.5102 1609.0058
2374 806.6921 1611.3696
2417 813.8973 1625.7800
2438 408.9974 1631.9605
2452 547.2938 1638.8596
2525 335.4091 1672.0091
2544 562.6558 1684.9456
2606 860.9242 1719.8338
2640 578.6675 1732.9807
2646 870.0407 1738.0668
2671 587.6918 1760.0536
2697 443.2043 1768.7881
2707 887.7344 1773.4542
2769 905.0254 1808.0362
2785 908.6807 1815.3468
2831 926.4989 1850.9832
2833 927.5302 1853.0458
2882 940.5042 1878.9938
2924 635.3671 1903.0795
2955 961.5347 1921.0548
2997 977.4805 1952.9464
3036 990.0415 1978.0684
3041 990.5400 1979.0654
3049 992.5804 1983.1462
3078 670.0604 2007.1594
3080 670.0673 2007.1801
3109 677.0067 2027.9983
3114 1016.5363 2031.0580
3116 678.3915 2032.1527
3153 684.0581 2049.1525
3163 686.3895 2056.1467
3188 692.7241 2075.1505
3189 520.0624 2076.2205
3190 693.0820 2076.2242
3227 699.3798 2095.1176
3233 1050.5382 2099.0618
3235 526.0366 2100.1173
3240 701.3827 2101.1263
3256 1058.0898 2114.1650
3296 710.4189 2128.2349
3297 710.7503 2129.2291
3298 711.3268 2130.9586
3338 1074.6554 2147.2962
3354 718.4156 2152.2250
3358 718.7499 2153.2279
3373 722.7231 2165.1475
3375 1084.2535 2166.4924
3382 1086.5813 2171.1480
3394 1088.6177 2175.2208
3395 726.0833 2175.2281
3397 1089.5354 2177.0562
3401 727.0818 2178.2236
3402 727.0820 2178.2242
3404 545.8253 2179.2721
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