MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
38 361.1997 360.1924
39 371.2543 370.2470
40 373.2210 372.2137
41 373.2454 372.2381
42 373.2570 372.2497
43 373.2832 372.2759
44 374.2294 373.2221
45 374.2297 373.2224
46 374.2407 373.2334
47 375.2261 374.2188
48 375.2372 374.2299
49 375.6940 374.6867
50 376.2639 375.2566
51 379.2333 378.2260
52 385.2579 384.2506
53 385.7489 384.7416
54 386.2411 385.2338
55 386.2414 385.2341
56 386.7203 385.7130
57 387.2729 386.2656
58 387.2731 386.2658
59 387.2740 386.2667
60 388.2573 387.2500
61 388.2573 387.2500
62 389.2407 388.2334
63 393.2485 392.2412
64 395.2647 394.2574
65 398.2464 397.2391
66 399.2373 398.2300
67 400.7184 399.7111
68 401.2885 400.2812
69 401.2887 400.2814
70 401.2887 400.2814
71 401.2897 400.2824
72 402.2477 401.2404
73 402.2484 401.2411
74 403.2318 402.2245
75 403.2318 402.2245
76 407.2282 406.2209
77 414.2726 413.2653
78 415.7494 414.7421
79 416.2632 415.2559
80 416.2637 415.2564
81 416.2879 415.2806
82 416.2882 415.2809
83 416.2886 415.2813
84 416.2903 415.2830
88 418.2679 417.2606
89 421.2585 420.2512
90 425.2391 424.2318
91 428.2884 427.2811
92 429.7402 428.7329
93 430.2784 429.2711
94 430.2794 429.2721
95 430.3041 429.2968
96 430.3042 429.2969
97 430.3043 429.2970
98 430.3050 429.2977
99 432.2585 431.2512
100 432.2594 431.2521
101 437.7463 436.7390
102 440.7305 439.7232
103 442.2663 441.2590
104 444.2946 443.2873
105 444.2948 443.2875
106 444.2953 443.2880
107 444.3187 443.3114
109 446.2747 445.2674
110 446.2753 445.2680
111 448.2562 447.2489
112 451.2606 450.2533
113 451.2702 450.2629
114 456.2946 455.2873
115 458.3109 457.3036
116 460.3144 459.3071
117 472.3268 471.3195
121 474.2824 473.2751
122 476.2114 475.2041
123 478.7806 477.7733
124 480.7648 479.7575
125 484.3286 483.3213
126 486.3429 485.3356
128 487.7915 486.7842
129 488.3071 487.2998
132 492.2948 491.2875
139 506.3112 505.3039
140 510.2377 509.2304
146 522.7803 521.7730
147 523.2792 522.2719
152 536.7972 535.7899
153 537.2986 536.2913
156 550.3010 549.2937
157 550.8240 549.8167
158 552.8262 551.8189
160 555.1061 554.0988
168 571.0704 570.0631
172 578.3439 577.3366
173 579.3013 578.2940
174 579.3189 578.3116
175 581.8298 580.8225
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