MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2242 778.4472 1554.8798 1554.8869 -4.52 1 25 0.35 +1Score > 36 indicates identity
Score > 33 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
633 408.7478 815.4810 815.4865 -6.70 0 25 0.79 +1Score > 41 indicates identity
Score > 37 indicates homology		 VTLQTVR
733 430.7427 859.4708 859.4651 6.72 0 25 0.1 +1Score > 43 indicates identity
Score > 28 indicates homology		 VSINDIAK + Deamidated (NQ)
240 313.1906 624.3666 624.3635 5.00 0 25 0.074 +1Score > 37 indicates identity
Score > 26 indicates homology		 FFIAK
357 687.4058 686.3985 686.3963 3.26 0 25 0.12 +1Score > 43 indicates identity
Score > 28 indicates homology		 QPTITK
541 388.2025 774.3904 774.3872 4.22 0 25 0.2 +1Score > 44 indicates identity
Score > 31 indicates homology		 NDGTVLR + Deamidated (NQ)
151 531.3536 530.3463 530.3428 6.67 0 25 0.67 +1Score > 38 indicates identity
Score > 36 indicates homology		 ITVAK
2636 866.9481 1731.8816 1731.8753 3.66 0 25 0.5 +1Score > 40 indicates identity
Score > 35 indicates homology		 IDIAILPIGGNFTMGAE + Deamidated (NQ)
1185 527.8411 1053.6676 1053.6546 12.4 1 25 0.25 +1Score > 32 indicates identity LLEQLGIIR
1167 521.7894 1041.5642 1041.5455 18.0 0 25 0.49 +1Score > 40 indicates identity
Score > 34 indicates homology		 QTQGPSKPAK + Deamidated (NQ)
1250 543.8264 1085.6382 1085.6233 13.7 0 25 0.95 +1Score > 38 indicates identity
Score > 37 indicates homology		 IQGIGAGFVPK
2129 751.4053 1500.7960 1500.8147 -12.4 1 25 0.31 +1Score > 40 indicates identity
Score > 32 indicates homology		 QELQALTAALSASAK
1947 471.5983 1411.7731 1411.7671 4.26 0 25 0.26 +1Score > 38 indicates identity
Score > 32 indicates homology		 LVAALANDDLQLR + Deamidated (NQ)
1621 625.3258 1248.6370 1248.6350 1.63 1 25 0.43 +1Score > 42 indicates identity
Score > 34 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
768 438.7525 875.4904 875.4865 4.53 1 25 0.6 +1Score > 40 indicates identity
Score > 35 indicates homology		 LFAEQIR
1869 688.8730 1375.7314 1375.7170 10.5 0 25 0.19 +1Score > 40 indicates identity
Score > 30 indicates homology		 DIMFGPIALGQSK
2740 897.9682 1793.9218 1793.9523 -17.0 1 25 0.26 +1Score > 40 indicates identity
Score > 31 indicates homology		 QQDIPVENVLALGGIAR + 2 Deamidated (NQ)
808 894.4719 893.4646 893.4607 4.42 1 25 0.68 +1Score > 41 indicates identity
Score > 36 indicates homology		 TVGGLEYR
305 330.2088 658.4030 658.4126 -14.5 1 25 0.38 +1Score > 40 indicates identity
Score > 33 indicates homology		 AKVSVR
2143 755.9494 1509.8842 1509.8654 12.5 0 25 0.2 +1Score > 33 indicates identity
Score > 30 indicates homology		 LIVLTDTPGILLNE
1769 663.3998 1324.7850 1324.7714 10.3 1 25 0.071 +1Score > 34 indicates identity
Score > 26 indicates homology		 LVLLDGQEVALR
337 674.3855 673.3782 673.3759 3.50 1 25 0.95 +1Score > 42 indicates identity
Score > 37 indicates homology		 EISVAR
1193 529.8055 1057.5964 1057.5880 8.02 1 24 0.31 +1Score > 41 indicates identity
Score > 32 indicates homology		 NNKANSLGLK
1619 416.8817 1247.6233 1247.6245 -0.98 1 24 0.94 +1Score > 42 indicates identity
Score > 37 indicates homology		 TTSDNEDLLIK
1517 606.3455 1210.6764 1210.6822 -4.76 1 24 0.93 +1Score > 38 indicates identity
Score > 37 indicates homology		 QSILLRYYR
206 603.3370 602.3297 602.3275 3.64 1 24 0.89 +1Score > 46 indicates identity
Score > 36 indicates homology		 EIDVK
578 395.2366 788.4586 788.4545 5.30 0 24 0.2 +1Score > 39 indicates identity
Score > 30 indicates homology		 LFGLSPR
290 326.1657 650.3168 650.3136 4.97 0 24 0.66 +1Score > 43 indicates identity
Score > 35 indicates homology		 NAFSGR
1094 505.7325 1009.4504 1009.4651 -14.5 1 24 0.75 +1Score > 40 indicates identity
Score > 36 indicates homology		 AYGAECLAR
909 465.7865 929.5584 929.5546 4.16 0 24 0.99 +1Score > 38 indicates identity
Score > 37 indicates homology		 TGLIQVATK
3564 752.4198 2254.2376 2254.1932 19.7 0 24 0.52 +1Score > 34 indicates identity QPIGVTAAITPWNFPAAMITR
629 407.7576 813.5006 813.4960 5.71 1 24 0.8 +1Score > 39 indicates identity
Score > 36 indicates homology		 LGAEVVVK
1719 651.8206 1301.6266 1301.6463 -15.1 1 24 0.87 +1Score > 41 indicates identity
Score > 36 indicates homology		 QADKDLLDIDR + Deamidated (NQ)
1743 659.3779 1316.7412 1316.7300 8.57 1 24 0.7 +1Score > 38 indicates identity
Score > 35 indicates homology		 ETLTAATLTGALR
464 732.4031 731.3958 731.3926 4.44 0 24 0.33 +1Score > 44 indicates identity
Score > 32 indicates homology		 QASASLR
227 308.6887 615.3628 615.3591 6.02 0 24 0.96 +1Score > 45 indicates identity
Score > 36 indicates homology		 AIVNAK + Deamidated (NQ)
119 473.3112 472.3039 472.3009 6.33 0 24 0.97 +1Score > 45 indicates identity
Score > 36 indicates homology		 IGGVK
2648 871.9972 1741.9798 1741.9475 18.6 0 24 0.52 +1Score > 35 indicates identity
Score > 34 indicates homology		 QLVQQGLGIFGGAIADR
1556 613.8487 1225.6828 1225.6779 4.06 1 24 0.46 +1Score > 38 indicates identity
Score > 33 indicates homology		 QLVAELQGNVR
2244 519.3017 1554.8833 1554.8729 6.64 1 24 0.29 +1Score > 36 indicates identity
Score > 31 indicates homology		 VAAISTIVNRGQTPK + Deamidated (NQ)
978 479.7860 957.5574 957.5607 -3.40 1 24 0.17 +1Score > 40 indicates identity
Score > 29 indicates homology		 ISLVNKQR + Deamidated (NQ)
2821 615.3290 1842.9652 1842.9985 -18.1 1 24 0.15 +1Score > 39 indicates identity
Score > 28 indicates homology		 NTTQIKAVDINLNMLR
1080 502.2584 1002.5022 1002.4982 4.07 0 24 0.94 +1Score > 42 indicates identity
Score > 36 indicates homology		 IANISGDGQK + Deamidated (NQ)
2258 781.4596 1560.9046 1560.9167 -7.71 0 24 0.23 +1Score > 33 indicates identity
Score > 30 indicates homology		 IFLFLAILLQQLE + Deamidated (NQ)
982 480.7921 959.5696 959.5804 -11.2 1 24 0.067 +1Score > 36 indicates identity
Score > 25 indicates homology		 KYIPALQK
1624 625.8328 1249.6510 1249.6740 -18.4 1 24 0.32 +1Score > 41 indicates identity
Score > 31 indicates homology		 VNELIYLVMR + Deamidated (NQ)
278 322.7036 643.3926 643.4017 -14.1 0 24 0.5 +1Score > 43 indicates identity
Score > 33 indicates homology		 IAAITR
537 386.7522 771.4898 771.4854 5.73 0 24 0.76 +1Score > 37 indicates identity
Score > 35 indicates homology		 IVLVNSK
669 419.2500 836.4854 836.4830 2.96 0 24 0.25 +1Score > 36 indicates identity
Score > 30 indicates homology		 LIAVYMK
2710 592.6227 1774.8463 1774.8196 15.0 1 24 0.073 +1Score > 40 indicates identity
Score > 25 indicates homology		 KMGATFDLDSGYIQGR + Deamidated (NQ); Oxidation (M)
1006 324.8393 971.4961 971.5036 -7.75 1 24 0.23 +1Score > 41 indicates identity
Score > 30 indicates homology		 SQHTSGVKK + Deamidated (NQ)
1088 503.2981 1004.5816 1004.5767 4.93 1 24 0.72 +1Score > 37 indicates identity
Score > 35 indicates homology		 RLFQAITR + Deamidated (NQ)
1868 687.8896 1373.7646 1373.7554 6.70 1 24 0.45 +1Score > 38 indicates identity
Score > 33 indicates homology		 LFQLLDGQLAEK
422 358.2084 714.4022 714.4024 -0.24 0 24 0.98 +1Score > 43 indicates identity
Score > 36 indicates homology		 ALGAQVR + Deamidated (NQ)
1508 403.1932 1206.5578 1206.5703 -10.4 0 24 0.27 +1Score > 41 indicates identity
Score > 31 indicates homology		 DLDMSFNPLR
1587 619.3337 1236.6528 1236.6390 11.2 1 24 0.055 +1Score > 40 indicates identity
Score > 24 indicates homology		 GAEYVVDFLPK
204 602.3902 601.3829 601.3799 5.01 0 24 0.48 +1Score > 41 indicates identity
Score > 33 indicates homology		 SVLVGK
457 365.2489 728.4832 728.4796 4.97 0 24 0.11 +1Score > 37 indicates identity
Score > 27 indicates homology		 VTIIVGK
920 470.2343 938.4540 938.4644 -11.0 0 24 0.22 +1Score > 40 indicates identity
Score > 30 indicates homology		 SALSCFVR
1923 701.3834 1400.7522 1400.7623 -7.20 1 24 0.11 +1Score > 40 indicates identity
Score > 27 indicates homology		 SVTQVNRDLLAGK + Deamidated (NQ)
1208 534.3309 1066.6472 1066.6499 -2.45 1 24 0.4 +1Score > 32 indicates identity LIAGLGNPGKK
1623 625.8324 1249.6502 1249.6740 -19.0 1 24 0.32 +1Score > 41 indicates identity
Score > 31 indicates homology		 VNELIYLVMR + Deamidated (NQ)
634 409.7178 817.4210 817.4156 6.62 0 24 0.57 +1Score > 44 indicates identity
Score > 34 indicates homology		 VMPSAWK
2171 506.9556 1517.8450 1517.8202 16.3 1 24 0.4 +1Score > 37 indicates identity
Score > 32 indicates homology		 VKFNVINTAVQGAR + 2 Deamidated (NQ)
1179 524.2665 1046.5184 1046.5033 14.5 0 24 0.38 +1Score > 42 indicates identity
Score > 32 indicates homology		 DNAPLQFDK
1134 342.8571 1025.5495 1025.5368 12.3 1 24 0.27 +1Score > 39 indicates identity
Score > 30 indicates homology		 AFKFPTMGK
1641 631.3564 1260.6982 1260.6826 12.4 1 24 0.42 +1Score > 39 indicates identity
Score > 32 indicates homology		 FQLITRNLQE
461 366.2196 730.4246 730.4337 -12.4 0 23 0.42 +1Score > 43 indicates identity
Score > 32 indicates homology		 AISTAIR
1622 625.8323 1249.6500 1249.6740 -19.2 1 23 0.32 +1Score > 41 indicates identity
Score > 31 indicates homology		 VNELIYLVMR + Deamidated (NQ)
1218 536.7882 1071.5618 1071.5560 5.47 1 23 0.95 +1Score > 42 indicates identity
Score > 36 indicates homology		 NIAQALNEAK + Deamidated (NQ)
2028 726.3387 1450.6628 1450.6861 -16.1 1 23 0.42 +1Score > 40 indicates identity
Score > 32 indicates homology		 TTAEVMVAVDVTSE
222 616.3431 615.3358 615.3340 2.94 0 23 0.56 +1Score > 44 indicates identity
Score > 33 indicates homology		 VSPSAR
462 366.2271 730.4396 730.4272 17.0 1 23 0.5 +1Score > 40 indicates identity
Score > 33 indicates homology		 VARMVR
748 434.2602 866.5058 866.4974 9.78 1 23 0.57 +1Score > 35 indicates identity
Score > 33 indicates homology		 HEILSLR
282 322.7216 643.4286 643.4269 2.77 0 23 0.27 +1Score > 33 indicates identity
Score > 30 indicates homology		 LSVVVK
1198 531.2945 1060.5744 1060.5665 7.47 0 23 0.87 +1Score > 41 indicates identity
Score > 35 indicates homology		 LGANAVVNFR + Deamidated (NQ)
1231 540.3033 1078.5920 1078.6022 -9.44 1 23 0.76 +1Score > 39 indicates identity
Score > 35 indicates homology		 IISQQFKSK + Deamidated (NQ)
1470 594.3202 1186.6258 1186.6346 -7.36 1 23 0.8 +1Score > 41 indicates identity
Score > 35 indicates homology		 AKASLIAQAWE
382 350.7230 699.4314 699.4279 5.07 0 23 0.77 +1Score > 41 indicates identity
Score > 34 indicates homology		 AAQLIGK
167 569.3072 568.2999 568.2969 5.31 0 23 0.99 +1Score > 38 indicates identity
Score > 36 indicates homology		 DVHAK
1117 339.5305 1015.5697 1015.5550 14.5 0 23 0.77 +1Score > 40 indicates identity
Score > 34 indicates homology		 ISNQLDVVK + Deamidated (NQ)
2245 778.4510 1554.8874 1554.8981 -6.82 1 23 0.34 +1Score > 36 indicates identity
Score > 31 indicates homology		 SRILNTLLSPAQLK + 2 Deamidated (NQ)
1245 543.7858 1085.5570 1085.5717 -13.5 0 23 0.8 +1Score > 41 indicates identity
Score > 35 indicates homology		 ASNPSIQVIR + 2 Deamidated (NQ)
1303 560.8058 1119.5970 1119.5924 4.15 0 23 0.77 +1Score > 41 indicates identity
Score > 34 indicates homology		 GAVVAYANDLK
2223 516.6023 1546.7851 1546.8025 -11.3 1 23 0.18 +1Score > 41 indicates identity
Score > 28 indicates homology		 TGIDLKGDIMALQR + Deamidated (NQ); Oxidation (M)
689 423.2504 844.4862 844.4807 6.61 1 23 0.71 +1Score > 40 indicates identity
Score > 34 indicates homology		 IIAEFPR
1440 587.3137 1172.6128 1172.6302 -14.8 0 23 0.61 +1Score > 42 indicates identity
Score > 33 indicates homology		 AALVSYLSGHR
1304 560.8073 1119.6000 1119.5924 6.84 1 23 0.51 +1Score > 41 indicates identity
Score > 32 indicates homology		 VAQLYLEQR + Deamidated (NQ)
2254 520.9509 1559.8309 1559.8096 13.6 1 23 0.5 +1Score > 39 indicates identity
Score > 32 indicates homology		 IFEQLNGAWDVLR
1323 565.7773 1129.5400 1129.5516 -10.2 1 23 0.28 +1Score > 41 indicates identity
Score > 30 indicates homology		 SPQWVRDNK + Deamidated (NQ)
544 388.6943 775.3740 775.3824 -10.8 0 23 0.99 +1Score > 43 indicates identity
Score > 35 indicates homology		 DATGSVAR
1489 599.3409 1196.6672 1196.6877 -17.1 0 23 0.9 +1Score > 38 indicates identity
Score > 35 indicates homology		 QNQILLQALR + Deamidated (NQ)
2592 856.9716 1711.9286 1711.9581 -17.2 1 23 0.39 +1Score > 38 indicates identity
Score > 31 indicates homology		 DNITVTVLGVKGNQVR
604 400.7492 799.4838 799.4803 4.42 0 23 0.32 +1Score > 40 indicates identity
Score > 30 indicates homology		 ANLAAIVK + Deamidated (NQ)
1105 508.2892 1014.5638 1014.5611 2.76 1 23 0.35 +1Score > 41 indicates identity
Score > 31 indicates homology		 AAGVKWDLR
1257 545.2835 1088.5524 1088.5713 -17.4 0 23 0.34 +1Score > 42 indicates identity
Score > 31 indicates homology		 QNQIVLTSGK + 2 Deamidated (NQ)
1715 434.2462 1299.7168 1299.7034 10.3 1 23 0.13 +1Score > 40 indicates identity
Score > 26 indicates homology		 QVGGLDEIILSR + Deamidated (NQ)
567 393.7466 785.4786 785.4759 3.47 0 23 0.47 +1Score > 41 indicates identity
Score > 32 indicates homology		 IAVTNLR
1033 491.7416 981.4686 981.4556 13.3 1 23 0.099 +1Score > 41 indicates identity
Score > 25 indicates homology		 WEQYVTR + Deamidated (NQ)
2186 765.4324 1528.8502 1528.8712 -13.7 1 23 0.068 +1Score > 37 indicates identity
Score > 23 indicates homology		 LQTAITLIDELTAK
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