MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3104 506.2624 2021.0205 2020.9928 13.7 0 3 0.83 +1Score > 39 indicates identity
Score > 15 indicates homology
DFMGFLHVDVLQNAIASK + Deamidated (NQ); Oxidation (M)
3405 546.0312 2180.0957 2180.0969 -0.57 1 3 0.78 +1Score > 39 indicates identity
Score > 14 indicates homology
MTQTIFVLNGPNLNMLGKR + 2 Deamidated (NQ); 2 Oxidation (M)
4430 1031.5311 3091.5715 3091.5925 -6.80 1 3 0.85 +1Score > 36 indicates identity
Score > 15 indicates homology
GIDYVQIPTTLLAQVDSSVGGKTGANLSTGK + 2 Deamidated (NQ)
1766 442.2665 1323.7777 1323.7874 -7.37 1 3 0.67 +1Score > 34 indicates identity
Score > 14 indicates homology
ISLTGIGIHTGKK
4823 1013.0303 4048.0921 4048.1279 -8.83 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
TIGPQTHLVKAGTPTMGGVLVLVSIAVSTLLWCDWGNR + Deamidated (NQ)
3900 837.4429 2509.3069 2509.3461 -15.7 0 3 1 +1Score > 37 indicates identity
Score > 15 indicates homology
LIASPGCNATATILAGAPLVASSLLE
3389 725.3593 2173.0561 2173.0633 -3.33 1 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
RPRTRPVAMNPVDHPMGGGE
2952 960.4979 1918.9812 1919.0000 -9.78 0 3 1 +1Score > 40 indicates identity
Score > 15 indicates homology
INPGLASHGGQVTLIDVVE + Deamidated (NQ)
4309 978.5117 2932.5133 2932.5077 1.91 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
LQTMLQLDGTTNIADDIGRQVLSFGAR
4586 832.9252 3327.6717 3327.6915 -5.94 1 3 0.78 +1Score > 35 indicates identity
Score > 14 indicates homology
VIFGTLVNIFGNLIFSQNIFELGIPNSGSSE + 2 Deamidated (NQ)
4608 840.9657 3359.8337 3359.8931 -17.7 1 3 0.56 +1Score > 30 indicates identity
Score > 13 indicates homology
YDQLLSFAWKVALPLSLLNLMLTGIVVVAR + Deamidated (NQ); Oxidation (M)
2641 867.5034 1732.9922 1732.9723 11.5 0 3 0.8 +1Score > 32 indicates identity
Score > 15 indicates homology
ALAPHGITTAQVLVTLE
2746 601.2755 1800.8047 1800.7762 15.8 1 3 0.94 +1Score > 38 indicates identity
Score > 15 indicates homology
NSQPNDGSEDSKPVQAE
2868 935.4646 1868.9146 1868.9501 -19.0 1 3 0.98 +1Score > 40 indicates identity
Score > 15 indicates homology
VLHQIAHAPMLEHAMR + Oxidation (M)
3557 563.8157 2251.2337 2251.2590 -11.2 1 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
VRLVDNGAVAFIPASFLHAVR
3139 1020.5004 2038.9862 2039.0245 -18.7 1 3 0.7 +1Score > 39 indicates identity
Score > 14 indicates homology
QGQETVAPNLVAPVMNKPK + 3 Deamidated (NQ); Oxidation (M)
3400 727.0789 2178.2149 2178.2008 6.46 1 3 0.56 +1Score > 33 indicates identity
Score > 13 indicates homology
LIQQLQQQALSEPAVVQKR + 2 Deamidated (NQ)
4200 940.7941 2819.3605 2819.3324 9.97 0 3 0.58 +1Score > 37 indicates identity
Score > 13 indicates homology
AQVSVDMFLFSSQYQDVASLSNLPR + 2 Deamidated (NQ); Oxidation (M)
4352 998.4971 2992.4695 2992.4272 14.1 1 3 0.97 +1Score > 37 indicates identity
Score > 15 indicates homology
IQINAFAEQTPVVMAPNMSVGVNLMWK + 5 Deamidated (NQ)
3725 787.7582 2360.2528 2360.2562 -1.45 1 3 0.83 +1Score > 36 indicates identity
Score > 15 indicates homology
RNILPMTPVWAPVPNLNADLK + 2 Deamidated (NQ)
2268 783.9700 1565.9254 1565.9001 16.2 1 3 0.76 +1Score > 31 indicates identity
Score > 14 indicates homology
RAQLGQLGLQIVNR + Deamidated (NQ)
3531 1119.5850 2237.1554 2237.1288 11.9 0 3 0.85 +1Score > 38 indicates identity
Score > 15 indicates homology
TDGPPPQAVSLTPGGVSSSTLNR
4000 874.4338 2620.2796 2620.2996 -7.63 1 3 0.76 +1Score > 38 indicates identity
Score > 14 indicates homology
FFGVNPLSQFMDEVNPLATLTHK + Oxidation (M)
3622 1150.1106 2298.2066 2298.2319 -11.0 0 3 0.78 +1Score > 37 indicates identity
Score > 14 indicates homology
NDQIVLIDDLITTGGSVLNAVK + Deamidated (NQ)
2524 558.3127 1671.9163 1671.9096 3.97 1 3 0.95 +1Score > 38 indicates identity
Score > 15 indicates homology
LAAKATSAWYQLPPR
4217 948.5532 2842.6378 2842.5891 17.1 1 3 1 +1Score > 26 indicates identity
Score > 15 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
3551 750.0939 2247.2599 2247.2362 10.5 1 3 0.8 +1Score > 32 indicates identity
Score > 14 indicates homology
NPSLHLVLVGQSSLLEQLVAK + 3 Deamidated (NQ)
3936 851.1235 2550.3487 2550.3008 18.8 0 3 0.87 +1Score > 36 indicates identity
Score > 15 indicates homology
AAMALMGLIFVQMAAGALVAGLDAGR + Deamidated (NQ); 2 Oxidation (M)
3191 520.0634 2076.2245 2076.1830 20.0 1 3 0.57 +1Score > 27 indicates identity
Score > 13 indicates homology
SDLVIGAVLIPGAKAPNLVTE
4946 887.4508 5318.6611 5318.6155 8.57 0 3 1 +1Score > 29 indicates identity
Score > 15 indicates homology
YPSYMNNNLIGLTAPLIPTPQLHFLMTGYTPLTSDSDIHTQQLVNVR + 3 Deamidated (NQ); 2 Oxidation (M)
3724 787.7580 2360.2522 2360.2562 -1.70 1 3 0.71 +1Score > 36 indicates identity
Score > 14 indicates homology
RNILPMTPVWAPVPNLNADLK + 2 Deamidated (NQ)
4335 744.6373 2974.5201 2974.5036 5.54 0 3 0.76 +1Score > 36 indicates identity
Score > 14 indicates homology
QVDVQPVHPQLNTSSLTKPVYNGGPVAE + Deamidated (NQ)
4012 878.4559 2632.3459 2632.3306 5.80 1 3 0.97 +1Score > 37 indicates identity
Score > 15 indicates homology
VQSYLTSTSSTFKLADTVILICGE
4690 894.4965 3573.9569 3573.8945 17.5 1 3 1 +1Score > 29 indicates identity
Score > 15 indicates homology
NNNIMNLIAAIVLSLSIIFGWQYFVVKPEHK + 3 Deamidated (NQ)
2844 929.0010 1855.9874 1855.9978 -5.60 1 3 0.79 +1Score > 38 indicates identity
Score > 14 indicates homology
KHDGQFTVIQLLGMLR + Deamidated (NQ)
3408 728.0410 2181.1012 2181.0623 17.8 1 3 0.67 +1Score > 39 indicates identity
Score > 14 indicates homology
SVVLQEALGSGNLLNYCQSK + 2 Deamidated (NQ)
3429 730.3863 2188.1371 2188.1297 3.38 0 3 0.7 +1Score > 38 indicates identity
Score > 14 indicates homology
MLASVLTGNDLVLVQGAGNIGK + 3 Deamidated (NQ); Oxidation (M)
3862 825.4351 2473.2835 2473.3098 -10.6 1 3 0.8 +1Score > 37 indicates identity
Score > 14 indicates homology
VTILGMTTTGDQILDSPLARIGGK + Deamidated (NQ); Oxidation (M)
4526 1075.5260 3223.5562 3223.5037 16.3 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
NPNIWSNFIEMGSMMLLPMSMLFLFGR + 3 Oxidation (M)
3094 672.7123 2015.1151 2015.1051 4.96 1 3 0.63 +1Score > 35 indicates identity
Score > 13 indicates homology
LAILQQRILQFAQDISR + 3 Deamidated (NQ)
3178 1031.0361 2060.0576 2060.0789 -10.3 0 3 0.94 +1Score > 39 indicates identity
Score > 15 indicates homology
ASILNALSFININLNSLNE + Deamidated (NQ)
3688 584.5817 2334.2977 2334.3059 -3.53 1 3 1 +1Score > 32 indicates identity
Score > 15 indicates homology
SIPLRAFSDGLNALLPPDIAVR
3783 803.0701 2406.1885 2406.1737 6.15 0 3 0.79 +1Score > 39 indicates identity
Score > 14 indicates homology
NSVLVDNGDLIQGSPLADYMSAK
3842 820.1220 2457.3442 2457.3235 8.40 1 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
AAALLRNIAMHASAMKPTVISYK + Deamidated (NQ)
4656 867.9825 3467.9009 3467.8666 9.88 1 3 0.75 +1Score > 29 indicates identity
Score > 14 indicates homology
FSYVIFGVMTVGFLLLVIPTVISELNGNPAIE + Oxidation (M)
4016 879.4167 2635.2283 2635.2258 0.95 1 3 0.56 +1Score > 37 indicates identity
Score > 13 indicates homology
QMGIESNSIDIMGLQAAYAQLHTE + Oxidation (M)
3083 1005.5595 2009.1044 2009.0793 12.5 0 3 1 +1Score > 35 indicates identity
Score > 15 indicates homology
GVPVATVAVNNATNAGLLAVR + 3 Deamidated (NQ)
1581 412.2288 1233.6646 1233.6466 14.6 0 3 0.97 +1Score > 40 indicates identity
Score > 15 indicates homology
QNFLQLQTSR
4596 836.2110 3340.8149 3340.7859 8.67 1 3 1 +1Score > 31 indicates identity
Score > 15 indicates homology
QLNIFGPHGAIKILSNALNVGYYTLHFPLK + 3 Deamidated (NQ)
3735 1182.5813 2363.1480 2363.1315 7.01 0 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
STMNVPFVTGQADLDAAVIQGAR + 3 Deamidated (NQ)
4094 907.8228 2720.4466 2720.4021 16.3 1 3 0.57 +1Score > 35 indicates identity
Score > 13 indicates homology
AIDFSSNDVLVGIAASGRTPYVIGGLE
4254 962.8055 2885.3947 2885.3455 17.1 1 3 0.79 +1Score > 37 indicates identity
Score > 14 indicates homology
GDDGLDELTPTTTSTIWVIPGGQPSAAGE + Deamidated (NQ)
4338 992.9235 2975.7487 2975.6922 19.0 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
YLTFRAILGVMTALGLSLLLGPWVINK + Deamidated (NQ); Oxidation (M)
2532 419.2607 1673.0137 1673.0464 -19.5 1 3 1 +1Score > 25 indicates identity
Score > 15 indicates homology
LRVLLAALADPRPLR
3739 790.4232 2368.2478 2368.2274 8.59 1 3 0.63 +1Score > 37 indicates identity
Score > 13 indicates homology
ALYQVVHNSNGDVVVVALNKVE + 2 Deamidated (NQ)
2137 752.4135 1502.8124 1502.8093 2.11 1 3 0.81 +1Score > 39 indicates identity
Score > 14 indicates homology
LSTLQDIAKDFPR
2611 862.0336 1722.0526 1722.0403 7.15 1 3 1 +1Score > 25 indicates identity
Score > 15 indicates homology
VNIVPVIAKADTLTLR
3452 1098.5830 2195.1514 2195.1330 8.43 1 3 0.84 +1Score > 37 indicates identity
Score > 14 indicates homology
MKSASIPIYLQQAIMNTLR + 2 Deamidated (NQ); Oxidation (M)
3225 699.3759 2095.1059 2095.1274 -10.3 1 3 1 +1Score > 37 indicates identity
Score > 15 indicates homology
LVAPVGSQDGQSQVLTLVER
3721 1181.1227 2360.2308 2360.2449 -5.97 1 3 0.75 +1Score > 37 indicates identity
Score > 14 indicates homology
SYSQMIEAAISPSILLHLYPK
4059 897.4587 2689.3543 2689.3303 8.93 1 3 0.82 +1Score > 37 indicates identity
Score > 14 indicates homology
MVDLGEAVGIIAAQSIGEPGTQLTMR + Deamidated (NQ); 2 Oxidation (M)
2498 834.4684 1666.9222 1666.9083 8.38 0 3 0.97 +1Score > 37 indicates identity
Score > 15 indicates homology
LLINGGTISPFFFNK
4030 885.4638 2653.3696 2653.3706 -0.40 1 3 0.78 +1Score > 37 indicates identity
Score > 14 indicates homology
LLSSNVVNDLMLLESLLYNLMAR + Deamidated (NQ); 2 Oxidation (M)
4143 925.1413 2772.4021 2772.3905 4.18 1 3 0.95 +1Score > 37 indicates identity
Score > 15 indicates homology
KVVMTNGIFDILHAGHVSYLANAQR + 3 Deamidated (NQ); Oxidation (M)
4374 1008.8642 3023.5708 3023.6286 -19.1 1 3 0.75 +1Score > 35 indicates identity
Score > 14 indicates homology
MYKIQLLSCIALTLILVTNSAPASSSTK + Deamidated (NQ)
4745 942.4931 3765.9433 3765.9189 6.49 1 3 0.62 +1Score > 33 indicates identity
Score > 13 indicates homology
QMKPGVKYVDLHIATHHYIGQLLIDFGLANGTAE + Deamidated (NQ); Oxidation (M)
2985 647.0383 1938.0931 1938.0574 18.4 1 3 0.97 +1Score > 33 indicates identity
Score > 15 indicates homology
KSVLDQVAAQAILHGFLE
3421 730.0249 2187.0529 2187.0483 2.07 1 3 0.73 +1Score > 39 indicates identity
Score > 14 indicates homology
YNNFFQKNNSNNLLNIVK + 4 Deamidated (NQ)
4353 998.4995 2992.4767 2992.5141 -12.5 0 3 0.75 +1Score > 37 indicates identity
Score > 14 indicates homology
FSKPTISPIQQQQQPQPQQPSSIIQR + 4 Deamidated (NQ)
3229 699.6870 2096.0392 2096.0724 -15.9 1 3 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
MPPRVPTAIHQLYLDSNK + Deamidated (NQ); Oxidation (M)
3620 766.7667 2297.2783 2297.2420 15.8 1 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
LTARLAGYSGSIQFIQVPFTK + Deamidated (NQ)
3697 781.0840 2340.2302 2340.2035 11.4 0 3 0.63 +1Score > 37 indicates identity
Score > 13 indicates homology
QVTDQVGSLGYVAFTLLMIAGR + 2 Deamidated (NQ)
3761 795.7185 2384.1337 2384.0867 19.7 0 3 0.92 +1Score > 39 indicates identity
Score > 15 indicates homology
NISVSTQLTTNQTNPSGQISYE + 3 Deamidated (NQ)
1402 580.3613 1158.7080 1158.6947 11.5 1 3 0.74 +1Score > 31 indicates identity
Score > 14 indicates homology
MLLSLKWLR
3033 989.5338 1977.0530 1977.0432 4.99 1 3 0.72 +1Score > 37 indicates identity
Score > 14 indicates homology
ALFGAAEPAQVEHLLQQR
3124 679.3714 2035.0924 2035.0738 9.12 1 3 0.63 +1Score > 37 indicates identity
Score > 13 indicates homology
FLNQLLHIVQHQKTVSE + 2 Deamidated (NQ)
3335 537.3033 2145.1841 2145.1430 19.2 0 3 0.84 +1Score > 34 indicates identity
Score > 14 indicates homology
AIDALPLGPNVSSLLGHSDLR + Deamidated (NQ)
4051 671.3895 2681.5289 2681.4898 14.6 1 3 1 +1Score > 28 indicates identity
Score > 15 indicates homology
MTKPIRNVAVLGATGSIGAAALDVLAR + Deamidated (NQ); Oxidation (M)
4193 703.1445 2808.5489 2808.5064 15.1 1 3 0.76 +1Score > 31 indicates identity
Score > 14 indicates homology
SLSLMVDNVKQLPLTMDAMILALHR
4418 1027.5364 3079.5874 3079.5359 16.7 1 3 0.79 +1Score > 35 indicates identity
Score > 14 indicates homology
YTGFPVSNLIASGPGMDGLLDGLCRLVIE + Oxidation (M)
3304 712.0677 2133.1813 2133.1430 17.9 1 3 0.86 +1Score > 34 indicates identity
Score > 14 indicates homology
SLGINTVVVHGGGPQINETLK + Deamidated (NQ)
3630 576.3126 2301.2213 2301.2257 -1.90 1 3 0.93 +1Score > 37 indicates identity
Score > 15 indicates homology
ATISPSPDLFTLVNVFGVAPEK
4372 1008.5192 3022.5358 3022.5135 7.38 1 3 0.85 +1Score > 36 indicates identity
Score > 14 indicates homology
RPSPPVNLTSSDQTQSSVQLKWEPPLK + 4 Deamidated (NQ)
4450 1041.5701 3121.6885 3121.6580 9.76 1 3 1 +1Score > 32 indicates identity
Score > 15 indicates homology
NAITVIIALGGSTNAVLHLIAMANAAEVNLK + 4 Deamidated (NQ); Oxidation (M)
2448 818.9904 1635.9662 1635.9420 14.8 0 3 0.81 +1Score > 30 indicates identity
Score > 14 indicates homology
GLVSRPDLGLQIAAAR
3446 732.0220 2193.0442 2193.0114 15.0 1 3 0.9 +1Score > 39 indicates identity
Score > 15 indicates homology
IGYATQFIGKDSNYTATVDE + Deamidated (NQ)
3448 549.3149 2193.2305 2193.2481 -8.02 1 3 0.74 +1Score > 33 indicates identity
Score > 14 indicates homology
RSLQADLALALLLDVQQGLR + Deamidated (NQ)
4222 950.8513 2849.5321 2849.5385 -2.27 1 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
VLSLQDNSLSAIEAGLLNNLPALDVLR + 2 Deamidated (NQ)
4265 724.1222 2892.4597 2892.4902 -10.6 1 3 1 +1Score > 37 indicates identity
Score > 15 indicates homology
MSILLSSNNPLNILNKGYSVIQDGTGK + Deamidated (NQ); Oxidation (M)
4705 907.4669 3625.8385 3625.8621 -6.51 1 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
MSLNQAASVNNLVLSPNTTLKDVALNALAGQVDAR + 2 Deamidated (NQ); Oxidation (M)
4523 1074.8838 3221.6296 3221.5669 19.4 1 3 0.83 +1Score > 36 indicates identity
Score > 14 indicates homology
NAEVQYAGPSGHIFDGLNFFILTDSNAPVK + Deamidated (NQ)
2285 787.3924 1572.7702 1572.8003 -19.1 1 3 0.96 +1Score > 40 indicates identity
Score > 15 indicates homology
NPNIPTAMKLAMVR + 2 Deamidated (NQ); Oxidation (M)
3383 1086.5933 2171.1720 2171.1871 -6.96 0 3 0.62 +1Score > 36 indicates identity
Score > 13 indicates homology
VVNLAADVSLVGSIVNVMITR + 2 Deamidated (NQ)
3610 764.3948 2290.1626 2290.1515 4.84 0 3 0.63 +1Score > 39 indicates identity
Score > 13 indicates homology
AQNSVGQAVISVSVMVVAYPPR + 3 Deamidated (NQ); Oxidation (M)
2970 966.0344 1930.0542 1930.0623 -4.16 1 3 0.95 +1Score > 36 indicates identity
Score > 15 indicates homology
VSTDAQLSGLTVVLTGKLE
4364 1006.5690 3016.6852 3016.7253 -13.3 1 3 1 +1Score > 29 indicates identity
Score > 15 indicates homology
VKTLSVPAFISGIFGVTEPAIYGVTLPLK
2774 604.9637 1811.8693 1811.8764 -3.93 1 3 0.96 +1Score > 40 indicates identity
Score > 15 indicates homology
YELPPVSVQGSSVMFR + Deamidated (NQ); Oxidation (M)
3947 852.7917 2555.3533 2555.3703 -6.66 1 3 0.86 +1Score > 35 indicates identity
Score > 14 indicates homology
DLAVGITKAMGAAVCGVDLIIPDLK + Oxidation (M)
4589 1111.9326 3332.7760 3332.7460 8.99 1 3 0.61 +1Score > 32 indicates identity
Score > 13 indicates homology
SHDLIFNQLYVVFKMIFWFNPALYISK
3776 800.0870 2397.2392 2397.2751 -15.0 1 3 0.97 +1Score > 37 indicates identity
Score > 15 indicates homology
GLDNAALSALASGLVSVAQLLQRE + 2 Deamidated (NQ)
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Not what you expected? Try the select summary.