MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3202 696.0287 2085.0643 2085.0677 -1.65 1 4 1 +1Score > 39 indicates identity
Score > 16 indicates homology
GLTLYGNVDIRTLNMSFR + Oxidation (M)
3424 730.0255 2187.0547 2187.0540 0.30 1 4 0.88 +1Score > 39 indicates identity
Score > 16 indicates homology
HVWVVGDIHGCFSMLMKR + Oxidation (M)
3746 475.4771 2372.3491 2372.3427 2.71 1 4 0.94 +1Score > 30 indicates identity
Score > 16 indicates homology
RFVSIINGDNLSLSIAAASIIAK
3423 547.7709 2187.0545 2187.0430 5.25 1 4 0.86 +1Score > 39 indicates identity
Score > 15 indicates homology
QNDAINGDIVAALLDDEATVK + 3 Deamidated (NQ)
3505 558.0598 2228.2101 2228.2338 -10.6 1 4 1 +1Score > 35 indicates identity
Score > 16 indicates homology
LIGDGTVTADAEVISLMVALLK
4349 748.4054 2989.5925 2989.5814 3.71 1 4 1 +1Score > 33 indicates identity
Score > 16 indicates homology
FDLNVSKSAWLTAIPLAQQFWQTVAR
3883 833.4324 2497.2754 2497.2682 2.86 1 4 1 +1Score > 38 indicates identity
Score > 16 indicates homology
RGIPVMGHIGLMPQQVQTAGGYR + 2 Oxidation (M)
4031 885.4672 2653.3798 2653.4301 -19.0 0 4 0.68 +1Score > 36 indicates identity
Score > 14 indicates homology
DVQALWVLAMAAAAAAGYHLLQLLK + Deamidated (NQ); Oxidation (M)
4579 662.5699 3307.8131 3307.7964 5.05 1 4 0.59 +1Score > 30 indicates identity
Score > 14 indicates homology
VLYILLLVVGFSLMCLELFHSSNVIDGLK + Oxidation (M)
3884 834.4113 2500.2121 2500.1870 10.0 1 4 0.63 +1Score > 38 indicates identity
Score > 14 indicates homology
QYALDTGNNPADFTQKNINTFK + Deamidated (NQ)
529 385.2570 768.4994 768.4858 17.8 0 4 0.69 +1Score > 31 indicates identity
Score > 14 indicates homology
LLPGTLR
3808 606.5967 2422.3577 2422.3835 -10.7 1 4 0.68 +1Score > 31 indicates identity
Score > 14 indicates homology
TKPVFVVATANNVDLLPLEIIR + Deamidated (NQ)
4144 925.5118 2773.5136 2773.4659 17.2 1 4 0.62 +1Score > 33 indicates identity
Score > 14 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
1227 1078.5715 1077.5642 1077.5567 7.01 0 4 1 +1Score > 41 indicates identity
Score > 16 indicates homology
KPLQNSHPR + 2 Deamidated (NQ)
3218 698.6942 2093.0608 2093.0290 15.2 1 4 0.79 +1Score > 39 indicates identity
Score > 15 indicates homology
IATGRIDFTSQNHGYAVSR + Deamidated (NQ)
2626 864.9793 1727.9440 1727.9127 18.1 0 4 0.7 +1Score > 38 indicates identity
Score > 14 indicates homology
ILSGLNIQMNLEPLR + 2 Deamidated (NQ); Oxidation (M)
4629 1134.6252 3400.8538 3400.9122 -17.2 0 4 0.58 +1Score > 30 indicates identity
Score > 14 indicates homology
NTLGAAFLTSPVFYGRPIAILPISLAASITAQK
1681 640.8901 1279.7656 1279.7500 12.3 1 4 0.97 +1Score > 33 indicates identity
Score > 16 indicates homology
SVHDILINLKK + Deamidated (NQ)
2984 969.0462 1936.0778 1936.0630 7.69 1 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
TTGSPLVIHRDVLVLTSE
3462 733.3951 2197.1635 2197.1338 13.5 1 4 0.51 +1Score > 37 indicates identity
Score > 13 indicates homology
SLTSAQLQRLTNLHGAASLSE + Deamidated (NQ)
2499 834.9318 1667.8490 1667.8454 2.22 1 4 0.88 +1Score > 40 indicates identity
Score > 15 indicates homology
RTLTYLSQVYCHK
3593 759.3490 2275.0252 2275.0249 0.11 0 4 0.89 +1Score > 37 indicates identity
Score > 16 indicates homology
WQGCDQMALVAQLSPGQLQE + Deamidated (NQ); Oxidation (M)
4530 809.1443 3232.5481 3232.5924 -13.7 1 4 0.58 +1Score > 36 indicates identity
Score > 14 indicates homology
TTGVLNDTFGGTLLYEPLMDVFECLIGLK + Deamidated (NQ); Oxidation (M)
3878 831.4350 2491.2832 2491.2588 9.79 1 4 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
TNSNNSTNSVLPKLMTSISLTPR + Deamidated (NQ); Oxidation (M)
3759 795.7164 2384.1274 2384.1583 -13.0 1 3 0.5 +1Score > 38 indicates identity
Score > 13 indicates homology
TMVVSYRVPHNAAVQVYDYR + Deamidated (NQ); Oxidation (M)
4805 803.3986 4011.9566 4011.9275 7.27 1 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
NILFLTLAGIYAYQLGADVVITGVCETDFSGYPDCR + Deamidated (NQ)
3223 699.3749 2095.1029 2095.0950 3.77 1 3 0.94 +1Score > 38 indicates identity
Score > 16 indicates homology
DRLTVLPDQSVVSYSLFR + Deamidated (NQ)
4464 628.3171 3136.5491 3136.5247 7.77 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
CDVSADQLDWLLLHQANQRILDAVADR + 2 Deamidated (NQ)
4658 870.7313 3478.8961 3478.8682 8.03 1 3 0.61 +1Score > 30 indicates identity
Score > 14 indicates homology
KITNVLCLAILPLSIMTSFFISMAFLYISR + Deamidated (NQ); Oxidation (M)
4061 897.8108 2690.4106 2690.4465 -13.4 1 3 0.97 +1Score > 36 indicates identity
Score > 16 indicates homology
VIQGFGGAALMSVNTALIRLIYPQR + 3 Deamidated (NQ)
624 407.2297 812.4448 812.4392 6.96 0 3 0.79 +1Score > 38 indicates identity
Score > 15 indicates homology
KPTQPNK + Deamidated (NQ)
1907 464.2934 1389.8584 1389.8344 17.3 1 3 1 +1Score > 26 indicates identity
Score > 16 indicates homology
VDLIKIGVPPPSR
2312 791.4268 1580.8390 1580.8661 -17.1 1 3 0.79 +1Score > 39 indicates identity
Score > 15 indicates homology
LGADAILLIGEALSPE
3398 727.0247 2178.0523 2178.0878 -16.3 1 3 0.93 +1Score > 40 indicates identity
Score > 16 indicates homology
DLIIQYGGSVSPSNIENLMK + Deamidated (NQ)
2856 933.5000 1864.9854 1864.9643 11.4 1 3 0.74 +1Score > 38 indicates identity
Score > 15 indicates homology
VSPKDNTQHNIIGVVSR + 2 Deamidated (NQ)
3847 821.7794 2462.3164 2462.2806 14.5 0 3 0.97 +1Score > 35 indicates identity
Score > 16 indicates homology
VLSQLGDNVVATGLLGGDFGDFIR
3840 818.7508 2453.2306 2453.2261 1.83 1 3 0.58 +1Score > 38 indicates identity
Score > 14 indicates homology
GGADVIELGIPFSDPMADGPVIQR
4509 533.6180 3195.6643 3195.6380 8.24 1 3 0.61 +1Score > 34 indicates identity
Score > 14 indicates homology
HRTPTDLSLMVLGNMVTNLINTSIAPAQR + Deamidated (NQ); 2 Oxidation (M)
4643 687.1544 3430.7356 3430.7330 0.76 1 3 0.58 +1Score > 35 indicates identity
Score > 14 indicates homology
STQVIRYIHQATQGQLPIIGVGGIFTLADAME + 4 Deamidated (NQ)
4499 1061.5271 3181.5595 3181.5689 -2.95 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
FHWQGLLSDYVQARTQLSGLILMMDAR + Deamidated (NQ); 2 Oxidation (M)
946 947.4979 946.4906 946.4720 19.7 1 3 0.93 +1Score > 42 indicates identity
Score > 16 indicates homology
SGDIETIGR
4969 999.0183 5988.0661 5987.9886 12.9 1 3 0.5 +1Score > 28 indicates identity
Score > 13 indicates homology
ATMMAIVQTIRDVVQFNQLFGQQINLLLHPSQNVIDNPVYLCDLVATLVQSAE + 5 Deamidated (NQ); Oxidation (M)
2083 741.9162 1481.8178 1481.8090 6.00 1 3 0.96 +1Score > 37 indicates identity
Score > 16 indicates homology
DNAVVIVDDKGIPK
3414 1092.0609 2182.1072 2182.0801 12.5 1 3 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
GRASITMPPVVSDHISSDGTR
1984 714.4263 1426.8380 1426.8143 16.6 1 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
IQQLAVSGLKVNR + 2 Deamidated (NQ)
4356 998.5125 2992.5157 2992.5150 0.21 0 3 0.84 +1Score > 36 indicates identity
Score > 15 indicates homology
ALCTQAMLVSHPGVNVLLLDDGLQHYK + Deamidated (NQ)
3814 809.4332 2425.2778 2425.2424 14.6 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
GTPGITGFVGAGMRPVPLSPDEVR + Oxidation (M)
4033 887.4483 2659.3231 2659.3011 8.27 1 3 1 +1Score > 38 indicates identity
Score > 16 indicates homology
MGNGLSLDEAGNLTSQNVTTVSPPLK + Deamidated (NQ); Oxidation (M)
353 684.3228 683.3155 683.3279 -18.1 1 3 0.7 +1Score > 37 indicates identity
Score > 14 indicates homology
FGKNAF + Deamidated (NQ)
3241 702.3784 2104.1134 2104.1350 -10.3 1 3 0.77 +1Score > 37 indicates identity
Score > 15 indicates homology
LASVLAKLMNLPAPNPDAPR + Deamidated (NQ); Oxidation (M)
2082 494.9464 1481.8174 1481.8242 -4.60 1 3 0.88 +1Score > 38 indicates identity
Score > 15 indicates homology
KITLFSIYSPASR
4243 958.8814 2873.6224 2873.5812 14.3 1 3 1 +1Score > 28 indicates identity
Score > 16 indicates homology
YPAMLQSMGRYLALGLIALPIAWLGR
2921 634.3517 1900.0333 1900.0054 14.7 1 3 0.73 +1Score > 37 indicates identity
Score > 14 indicates homology
QYNAVIPGIVQQKANAGK + 2 Deamidated (NQ)
3565 564.7795 2255.0889 2255.0681 9.24 1 3 0.85 +1Score > 39 indicates identity
Score > 15 indicates homology
AQNIAENANVSLHFAWLPME + Deamidated (NQ)
4067 898.4633 2692.3681 2692.3391 10.8 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
4427 772.6821 3086.6993 3086.6607 12.5 1 3 0.92 +1Score > 30 indicates identity
Score > 15 indicates homology
SNTLAGQEVLVNLLLALLLDVMTVSGMQK + Deamidated (NQ); Oxidation (M)
2885 940.5091 1879.0036 1878.9795 12.9 1 3 1 +1Score > 38 indicates identity
Score > 16 indicates homology
VKTGVLNDVISQAIMMK + Deamidated (NQ); 2 Oxidation (M)
3439 731.7064 2192.0974 2192.0671 13.8 0 3 0.82 +1Score > 39 indicates identity
Score > 15 indicates homology
VLPTPLMNILNGGVHADNGLE + 3 Deamidated (NQ); Oxidation (M)
4404 1021.8825 3062.6257 3062.5780 15.6 0 3 0.53 +1Score > 33 indicates identity
Score > 13 indicates homology
LIPSGILHNNVTCLIGNGVVLSPSALMQE + Deamidated (NQ); Oxidation (M)
3960 857.1492 2568.4258 2568.3985 10.6 1 3 1 +1Score > 31 indicates identity
Score > 16 indicates homology
AAAQGLMAGINAVLALKNQDPFILK + 2 Deamidated (NQ)
4319 985.5289 2953.5649 2953.5332 10.7 1 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
VQQDIVMLINHFGGQAVGLTGKDGGLIR + 2 Deamidated (NQ); Oxidation (M)
3867 620.7961 2479.1553 2479.1584 -1.25 0 3 0.69 +1Score > 38 indicates identity
Score > 14 indicates homology
TSNAKPFHSDLMNGTRPGMASTK + 2 Oxidation (M)
4788 783.9772 3914.8496 3914.9084 -15.0 1 3 0.57 +1Score > 33 indicates identity
Score > 13 indicates homology
TIAAGNTVAKDHAAAIGPTLLHDWIQFGGSTMFGAAMR + 2 Deamidated (NQ); Oxidation (M)
4341 995.5064 2983.4974 2983.5291 -10.6 1 3 0.6 +1Score > 36 indicates identity
Score > 14 indicates homology
HSPAQVPGTAIFLTSDPESAPAALLHNLK + 2 Deamidated (NQ)
2542 562.2924 1683.8554 1683.8356 11.8 1 3 0.62 +1Score > 40 indicates identity
Score > 14 indicates homology
NGQVFITNLATFTEK + 2 Deamidated (NQ)
3185 692.7150 2075.1232 2075.0899 16.0 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
ATDLDVFLKGGAALDINSVR + Deamidated (NQ)
4216 948.5512 2842.6318 2842.5891 15.0 1 3 1 +1Score > 26 indicates identity
Score > 16 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
4111 913.5101 2737.5085 2737.4659 15.5 1 3 0.76 +1Score > 32 indicates identity
Score > 15 indicates homology
LSVGAHHGALAEGLATFMVVMVSVTLK
3875 830.7996 2489.3770 2489.3563 8.31 1 3 0.51 +1Score > 32 indicates identity
Score > 13 indicates homology
ALQQLKAMGYLLVLVTNQSGIAR + 3 Deamidated (NQ)
3087 671.0401 2010.0985 2010.0898 4.30 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
LASIKGLHDIGTFVAPDTR
3126 1018.9982 2035.9818 2035.9996 -8.74 1 3 0.95 +1Score > 40 indicates identity
Score > 15 indicates homology
MNINRQTAFITGANQGIGK + 3 Deamidated (NQ)
4149 925.8427 2774.5063 2774.4557 18.2 1 3 1 +1Score > 33 indicates identity
Score > 16 indicates homology
LAGILDQLTTRIQNAVSSLMLNMLR + 4 Deamidated (NQ)
3066 999.0664 1996.1182 1996.1080 5.12 0 3 0.9 +1Score > 34 indicates identity
Score > 15 indicates homology
LVALGAIVMAYLHGINWR
3471 551.7764 2203.0765 2203.0500 12.0 1 3 1 +1Score > 39 indicates identity
Score > 16 indicates homology
QQLEMPLVLDQDLTQQMR + 2 Deamidated (NQ); Oxidation (M)
238 624.3074 623.3001 623.2915 13.9 0 3 0.98 +1Score > 39 indicates identity
Score > 16 indicates homology
AGFDSK
3898 837.1111 2508.3115 2508.3298 -7.31 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
VVWLEINTQPGMTPTSLVPDIAK
3930 849.4548 2545.3426 2545.3284 5.57 1 3 0.53 +1Score > 36 indicates identity
Score > 13 indicates homology
AIGLGEDALAFACTVLKPGGNMVIK + Deamidated (NQ)
3257 529.7653 2115.0321 2115.0531 -9.92 1 3 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
ASQAVLVYGQMNEPPGARAR + Deamidated (NQ)
3798 605.3187 2417.2457 2417.2584 -5.27 1 3 0.68 +1Score > 37 indicates identity
Score > 14 indicates homology
TLGIGKAIAVLTSGGDAQGMNATVR + Deamidated (NQ); Oxidation (M)
2123 750.9474 1499.8802 1499.8712 6.06 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
KQVINIDFPVISK
3243 1055.0184 2108.0222 2108.0174 2.29 1 3 0.84 +1Score > 40 indicates identity
Score > 15 indicates homology
TLKPNVNPGEGFQSGHAPQK + 3 Deamidated (NQ)
3600 760.4005 2278.1797 2278.1780 0.74 1 3 0.95 +1Score > 38 indicates identity
Score > 15 indicates homology
ADIAFMTLTLPDPGAYGRVVR + Oxidation (M)
3825 813.4600 2437.3582 2437.3441 5.77 1 3 0.95 +1Score > 31 indicates identity
Score > 15 indicates homology
LIEGYLNNRPNLAGIVQIVDAR
4178 934.1570 2799.4492 2799.4187 10.9 1 3 0.74 +1Score > 36 indicates identity
Score > 14 indicates homology
LQDLSFQAASQIMSAPVYDAIKLMK + 2 Oxidation (M)
4336 744.8868 2975.5181 2975.4918 8.83 1 3 0.78 +1Score > 36 indicates identity
Score > 15 indicates homology
LYGNLGMLAPVKAANPGMQIAVGGCLAQK + 2 Deamidated (NQ); 2 Oxidation (M)
3919 842.1393 2523.3961 2523.4313 -13.9 1 3 0.87 +1Score > 32 indicates identity
Score > 15 indicates homology
YGVAVPVVVIGNLVAGGTGKTPLTIE
4093 907.8199 2720.4379 2720.4358 0.76 1 3 0.6 +1Score > 35 indicates identity
Score > 13 indicates homology
TSQPGGAALPGLRSPLPPEPARPGGPSR + Deamidated (NQ)
2144 756.4055 1510.7964 1510.7739 14.9 1 3 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
QVSNLLKDHQATR + 2 Deamidated (NQ)
3597 760.0576 2277.1510 2277.1324 8.16 1 3 0.85 +1Score > 39 indicates identity
Score > 15 indicates homology
NPQSVNIHCLKAHSPLASFR + 2 Deamidated (NQ)
4626 849.9651 3395.8313 3395.8123 5.61 1 3 0.52 +1Score > 31 indicates identity
Score > 13 indicates homology
AFHSMGTTLSDGTAKLILNIPLNAQSLPITVR + Deamidated (NQ); Oxidation (M)
4041 891.5249 2671.5529 2671.5056 17.7 1 3 0.73 +1Score > 25 indicates identity
Score > 14 indicates homology
MAPAVTRLLFLQLVLGPTLVMDIK + Deamidated (NQ); 2 Oxidation (M)
4334 744.6348 2974.5101 2974.5400 -10.0 1 3 0.84 +1Score > 36 indicates identity
Score > 15 indicates homology
VAVGSVFRQLYQLPLSSADIALNGQQAE + Deamidated (NQ)
3673 775.4208 2323.2406 2323.2800 -17.0 1 3 0.89 +1Score > 36 indicates identity
Score > 15 indicates homology
IGIINLHPALPGAFNGIHAIER + Deamidated (NQ)
3618 766.4334 2296.2784 2296.2580 8.89 1 3 1 +1Score > 33 indicates identity
Score > 16 indicates homology
LTARLAGYSGSIQFIQVPFTK
3232 700.0081 2097.0025 2096.9724 14.3 1 3 0.82 +1Score > 39 indicates identity
Score > 15 indicates homology
AEALIMTFSLNNYPAGDPR + 2 Deamidated (NQ); Oxidation (M)
2514 557.3371 1668.9895 1668.9662 14.0 1 3 0.72 +1Score > 30 indicates identity
Score > 14 indicates homology
KLSVDLTAPLGVLQSK + Deamidated (NQ)
4048 892.8141 2675.4205 2675.4172 1.21 1 3 0.74 +1Score > 35 indicates identity
Score > 14 indicates homology
IEMIFVIFFIAVTFISILTTGDGE
2971 966.0425 1930.0704 1930.0775 -3.65 1 3 0.83 +1Score > 34 indicates identity
Score > 15 indicates homology
LLSNPLLQTYLPNSTKK + Deamidated (NQ)
2989 648.3543 1942.0411 1942.0195 11.1 0 3 0.76 +1Score > 37 indicates identity
Score > 14 indicates homology
NYLVMISLALLFMIGVE + Deamidated (NQ); Oxidation (M)
4021 882.0936 2643.2590 2643.2412 6.71 1 3 0.58 +1Score > 38 indicates identity
Score > 13 indicates homology
QVQPQVQPQAHSQGPRQVQLQQE + 6 Deamidated (NQ)
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Not what you expected? Try the select summary.