MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4207 708.3746 2829.4693 2829.4695 -0.055 1 4 0.67 +1Score > 36 indicates identity
Score > 15 indicates homology
MATILQGVDNLYEIDISRPVLDAAGR
1483 399.2360 1194.6862 1194.6682 15.0 0 4 0.4 +1Score > 36 indicates identity
Score > 13 indicates homology
GIYVIISGMVK + Oxidation (M)
3410 546.2834 2181.1045 2181.1088 -1.97 1 4 0.82 +1Score > 39 indicates identity
Score > 16 indicates homology
CCFALPLRWAPGRPLDPR
3571 566.5443 2262.1481 2262.1137 15.2 0 4 0.8 +1Score > 39 indicates identity
Score > 16 indicates homology
IRPGDMVLLSPACASLDQFR + Deamidated (NQ); Oxidation (M)
2451 820.4281 1638.8416 1638.8253 9.97 0 4 0.49 +1Score > 40 indicates identity
Score > 14 indicates homology
YIAAGDVFQAVLSQR + 2 Deamidated (NQ)
4442 1037.5709 3109.6909 3109.7501 -19.0 1 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
IIVQMAIIASLVIVVDQFLRAYAFSISK + 2 Deamidated (NQ)
3639 1153.6492 2305.2838 2305.2529 13.4 1 4 0.97 +1Score > 32 indicates identity
Score > 17 indicates homology
KPDGLLPTLGGQTGLNLAVELAE
2476 552.9518 1655.8336 1655.8294 2.52 1 4 0.66 +1Score > 40 indicates identity
Score > 15 indicates homology
LEFYNVDDLTSIVK + Deamidated (NQ)
2673 587.9521 1760.8345 1760.8668 -18.3 1 4 0.89 +1Score > 40 indicates identity
Score > 16 indicates homology
FNRYASMAIQQYLR + Deamidated (NQ)
2635 578.2637 1731.7693 1731.7846 -8.85 1 4 0.62 +1Score > 39 indicates identity
Score > 15 indicates homology
QVDGQKIHSSTMNNR + 2 Deamidated (NQ); Oxidation (M)
3549 750.0929 2247.2569 2247.2667 -4.38 0 4 0.71 +1Score > 32 indicates identity
Score > 15 indicates homology
YLLGLAVFSGIGLWLGNVGLGK + Deamidated (NQ)
3077 1004.5853 2007.1560 2007.1405 7.77 1 4 0.78 +1Score > 29 indicates identity
Score > 16 indicates homology
IVLGIDPALGSLGWAVVAKE
2679 589.0325 1764.0757 1764.0509 14.0 0 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
VTLPQAIQSLLSRPLK + Deamidated (NQ)
4936 1047.3127 5231.5271 5231.5076 3.73 1 4 1 +1Score > 30 indicates identity
Score > 17 indicates homology
VNGHIIDDIMHDIMVKPLPQGCRLTALYDSCHSGTALDLPYVYSTK + 2 Deamidated (NQ); Oxidation (M)
3351 1077.0681 2152.1216 2152.0913 14.1 1 4 0.66 +1Score > 38 indicates identity
Score > 15 indicates homology
YLLNVADGGSSADRQLAVFR + Deamidated (NQ)
3696 1170.5741 2339.1336 2339.1355 -0.79 1 4 0.99 +1Score > 39 indicates identity
Score > 17 indicates homology
YPLSFVNCVNSIGNGLVIKDE + 2 Deamidated (NQ)
3836 818.4144 2452.2214 2452.2169 1.84 1 4 0.56 +1Score > 38 indicates identity
Score > 14 indicates homology
EMLVLQQLAFNQQQSQAYAAR + Oxidation (M)
4737 932.5032 3725.9837 3725.9142 18.6 0 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
QYIWTQPLQMFWPGLALFISVMAFNLVGDALR + Deamidated (NQ)
2035 728.9366 1455.8586 1455.8409 12.2 1 4 0.73 +1Score > 34 indicates identity
Score > 15 indicates homology
VSVTVLGVKGNQVR + Deamidated (NQ)
4651 862.4622 3445.8197 3445.8555 -10.4 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
GTVQISGAKNSAVAVLPAALLADSPSVIDNLPDIK + 2 Deamidated (NQ)
2663 878.4263 1754.8380 1754.8587 -11.8 0 4 0.77 +1Score > 40 indicates identity
Score > 16 indicates homology
AQVSSWSDLDIQHLR + Deamidated (NQ)
3095 1009.5167 2017.0188 2016.9925 13.0 1 4 0.97 +1Score > 39 indicates identity
Score > 17 indicates homology
GSVELAPLTGMADAIVDITE + Oxidation (M)
4007 877.4659 2629.3759 2629.3567 7.28 1 4 0.46 +1Score > 36 indicates identity
Score > 13 indicates homology
NVLAVAIGVADGMQLGMNARAGLITR + 3 Deamidated (NQ); Oxidation (M)
4887 749.3785 4490.2273 4490.2520 -5.48 0 4 0.75 +1Score > 31 indicates identity
Score > 15 indicates homology
LLLYVLQQVSLFSNVDLTNLDVSLGSQSQFSFNDIDLNVK + 5 Deamidated (NQ)
3603 761.3999 2281.1779 2281.2166 -17.0 0 4 0.93 +1Score > 38 indicates identity
Score > 16 indicates homology
VAVTLSGQNVLDDIQLSVKPGE
3280 708.0690 2121.1852 2121.1728 5.82 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
IAIISQGKLQCIGTQNHLK
4082 902.4838 2704.4296 2704.3856 16.3 1 4 1 +1Score > 35 indicates identity
Score > 17 indicates homology
VASLMYTVISPMLNPFIYTLRNK + 2 Deamidated (NQ); 2 Oxidation (M)
4145 694.3875 2773.5209 2773.4657 19.9 1 4 0.43 +1Score > 32 indicates identity
Score > 13 indicates homology
LQSDPKPAVAAAAHVSAQQVAMLARAR + Deamidated (NQ); Oxidation (M)
4321 986.5366 2956.5880 2956.5982 -3.45 1 4 0.78 +1Score > 33 indicates identity
Score > 15 indicates homology
NIGIIAHVDHGKTTTSDQLLAASGIISPK
2956 961.9526 1921.8906 1921.9057 -7.85 1 4 0.92 +1Score > 39 indicates identity
Score > 16 indicates homology
NKGAIPYAYTANLGQPDE + Deamidated (NQ)
3211 1046.0532 2090.0918 2090.0756 7.76 1 4 0.47 +1Score > 38 indicates identity
Score > 13 indicates homology
VKASDLQFSSQVQVIDGNR
3341 717.0183 2148.0331 2148.0238 4.31 1 4 0.75 +1Score > 39 indicates identity
Score > 15 indicates homology
MAAPMNGQVCVVTGASRGIGR + Deamidated (NQ); Oxidation (M)
4679 889.4652 3553.8317 3553.7657 18.6 1 4 0.48 +1Score > 33 indicates identity
Score > 13 indicates homology
VDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQE + 2 Deamidated (NQ)
2950 959.5469 1917.0792 1917.0585 10.8 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
LTVSHHKLTASGHALFAK
4211 946.1719 2835.4939 2835.4576 12.8 1 4 0.96 +1Score > 35 indicates identity
Score > 16 indicates homology
VTDIVVLVVAADDGVMPQTIEAINHAK + 2 Deamidated (NQ); Oxidation (M)
3148 682.3737 2044.0993 2044.1278 -14.0 1 4 0.61 +1Score > 37 indicates identity
Score > 14 indicates homology
KLNLNAVIFMPVTTPLQK + 2 Deamidated (NQ); Oxidation (M)
4846 1027.5343 4106.1081 4106.0580 12.2 0 4 1 +1Score > 32 indicates identity
Score > 17 indicates homology
MAPWMHLLTVLALLALWGPNSVQAYSSQHLCGSNLVE
1386 1154.6384 1153.6311 1153.6455 -12.5 0 4 0.91 +1Score > 39 indicates identity
Score > 16 indicates homology
SIHIVDTTLR
3496 1111.5977 2221.1808 2221.1426 17.2 0 4 0.49 +1Score > 37 indicates identity
Score > 13 indicates homology
QALMPVILQDAPSAPGHAPHR + Oxidation (M)
3844 616.0817 2460.2977 2460.3298 -13.0 1 4 0.6 +1Score > 36 indicates identity
Score > 14 indicates homology
MAAKLDSQLLGLVFQSRPDILK + 2 Deamidated (NQ); Oxidation (M)
4245 959.5076 2875.5010 2875.5378 -12.8 1 4 0.52 +1Score > 35 indicates identity
Score > 14 indicates homology
ADHFRLGSVLVMLLQQPDLLPSAAQR + Deamidated (NQ)
1009 972.5144 971.5071 971.5148 -7.92 1 4 0.93 +1Score > 41 indicates identity
Score > 16 indicates homology
NLGPSKSNR
4889 901.1135 4500.5311 4500.4788 11.6 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
VPYIIIGQLATIFYFSYFMILLPLISILENMLFYLAIK + Deamidated (NQ); Oxidation (M)
2373 806.4398 1610.8650 1610.8603 2.95 0 4 0.71 +1Score > 38 indicates identity
Score > 15 indicates homology
LLGGHMLDQFLPVR + Oxidation (M)
3102 674.0570 2019.1492 2019.1503 -0.57 1 4 0.91 +1Score > 31 indicates identity
Score > 16 indicates homology
YLTQDLITKQLLDTIIK + Deamidated (NQ)
2140 753.9330 1505.8514 1505.8752 -15.8 0 4 0.47 +1Score > 35 indicates identity
Score > 13 indicates homology
LLDLLHLQLICR
3743 594.0934 2372.3445 2372.3427 0.76 1 4 0.59 +1Score > 30 indicates identity
Score > 14 indicates homology
RFISIVNGDNLSLSIAAASIIAK
2965 643.0225 1926.0457 1926.0608 -7.87 1 4 0.87 +1Score > 36 indicates identity
Score > 16 indicates homology
TDIPIIKTLSVPAGMGASR
4358 750.9000 2999.5709 2999.5201 16.9 1 4 0.7 +1Score > 34 indicates identity
Score > 15 indicates homology
LSANILFDVTLAQEIYAASDIFLMPSR + 2 Deamidated (NQ)
4645 859.1857 3432.7137 3432.7520 -11.2 1 4 0.53 +1Score > 35 indicates identity
Score > 14 indicates homology
AVGTGTSLALSSLLSLLLFAGMQMYSRQLASTE + Deamidated (NQ); Oxidation (M)
4901 771.8840 4625.2603 4625.2142 9.99 1 4 1 +1Score > 31 indicates identity
Score > 16 indicates homology
SLAANLNSVKCSGGNYNFQMPVGAWPVMSGGAVSLHFAGVTLSTE
429 718.3803 717.3730 717.3657 10.2 1 4 0.54 +1Score > 43 indicates identity
Score > 14 indicates homology
ISVRDE
2788 455.4707 1817.8537 1817.8175 19.9 1 4 0.92 +1Score > 40 indicates identity
Score > 16 indicates homology
AVYNSINMSINTSEMK + Deamidated (NQ); Oxidation (M)
3911 840.7774 2519.3104 2519.2916 7.44 1 4 0.8 +1Score > 37 indicates identity
Score > 16 indicates homology
AVWQVYQAVELPLIGMGGIMTAR + Deamidated (NQ); Oxidation (M)
3132 680.3348 2037.9826 2037.9967 -6.92 1 4 0.78 +1Score > 40 indicates identity
Score > 15 indicates homology
NAQAHLKDTQLQLDDAVR + 3 Deamidated (NQ)
4590 834.4584 3333.8045 3333.7793 7.55 0 4 0.65 +1Score > 31 indicates identity
Score > 15 indicates homology
VQLINQPAHTAPQLIRPAAPAHLSSDSTSSVK
3157 685.0466 2052.1180 2052.1215 -1.71 0 4 0.8 +1Score > 36 indicates identity
Score > 15 indicates homology
IDLTANGTLPGIIQANATLR + Deamidated (NQ)
3851 822.7817 2465.3233 2465.3563 -13.4 1 4 0.78 +1Score > 35 indicates identity
Score > 15 indicates homology
LSAVGLLPAALQGIDIQAMLAGAKE + Oxidation (M)
4267 724.1238 2892.4661 2892.4981 -11.1 1 4 0.62 +1Score > 36 indicates identity
Score > 14 indicates homology
DIVASTHKNGVIDNIGGFSAHIAIDVTK + Deamidated (NQ)
4555 819.4343 3273.7081 3273.6803 8.50 1 4 1 +1Score > 33 indicates identity
Score > 16 indicates homology
TSVGASITLTVSTGPSLVRVPVITGMQWSQAE + 2 Deamidated (NQ)
4843 821.4197 4102.0621 4102.1060 -10.7 1 4 0.51 +1Score > 33 indicates identity
Score > 14 indicates homology
TQLGMQPAALGLINVIAMIAGVFGAFSWSFVSRTFNLR + 3 Deamidated (NQ); Oxidation (M)
3228 524.8177 2095.2417 2095.2001 19.9 1 4 0.62 +1Score > 26 indicates identity
Score > 14 indicates homology
SAVNDLTLIAGQKPVLTRAK + Deamidated (NQ)
3803 484.6596 2418.2616 2418.2583 1.35 1 4 0.84 +1Score > 37 indicates identity
Score > 16 indicates homology
NNIEYDFITLHVGLGTFLPVR + Deamidated (NQ)
4277 967.8440 2900.5102 2900.4919 6.29 1 4 0.45 +1Score > 35 indicates identity
Score > 13 indicates homology
AGADYLVLGRPIYAADDPKAAAQAIIQE + Deamidated (NQ)
2888 941.0071 1879.9996 1880.0115 -6.32 1 4 0.51 +1Score > 38 indicates identity
Score > 13 indicates homology
AQAAQPAAKGAGSKPQGLTK + Deamidated (NQ)
2645 579.3627 1735.0663 1735.0356 17.7 1 4 1 +1Score > 25 indicates identity
Score > 16 indicates homology
QLLLSAAVPAGKIAIDR
3249 704.3750 2110.1032 2110.0735 14.1 1 4 0.64 +1Score > 38 indicates identity
Score > 14 indicates homology
QALSVQDPIIKNFFGTFGE
3486 1106.5425 2211.0704 2211.0605 4.49 1 4 0.82 +1Score > 39 indicates identity
Score > 15 indicates homology
DVPKMTDFGLPLPHMGWNR + Deamidated (NQ)
3908 839.0936 2514.2590 2514.2689 -3.96 0 4 0.56 +1Score > 38 indicates identity
Score > 14 indicates homology
GAINWDLMPQISIGAAGWTTLGSR
3106 1012.0254 2022.0362 2022.0608 -12.1 0 4 0.96 +1Score > 39 indicates identity
Score > 16 indicates homology
LGLQVMNISQFFANALQK + Deamidated (NQ)
4198 704.8515 2815.3769 2815.3660 3.89 1 4 0.7 +1Score > 37 indicates identity
Score > 15 indicates homology
DSPVMALLLQQFKDNIQDLMSYTK + 2 Deamidated (NQ); Oxidation (M)
2914 947.4978 1892.9810 1892.9731 4.19 1 4 0.95 +1Score > 39 indicates identity
Score > 16 indicates homology
ITAATKFAALGADSLDTVE
4357 750.6507 2998.5737 2998.5548 6.31 1 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
YDFPPFMVLIIAILNDGTIMTISKDR + Oxidation (M)
3477 1103.6432 2205.2718 2205.2773 -2.45 1 4 0.92 +1Score > 29 indicates identity
Score > 16 indicates homology
NVIDQALIKPAKYVPITPPK + Deamidated (NQ)
3915 631.5927 2522.3417 2522.3396 0.84 1 4 0.55 +1Score > 35 indicates identity
Score > 14 indicates homology
RPFFKGLVDFITSGPVIAMIWE
3250 705.0610 2112.1612 2112.1289 15.3 1 4 0.49 +1Score > 34 indicates identity
Score > 13 indicates homology
LVLLIADNSYVSIYCKNK
3330 537.0284 2144.0845 2144.0606 11.2 0 4 0.91 +1Score > 39 indicates identity
Score > 16 indicates homology
SMCAPLPVAAQSTTLPSLSGR + Deamidated (NQ)
4545 813.9372 3251.7197 3251.7293 -2.96 0 4 0.48 +1Score > 33 indicates identity
Score > 13 indicates homology
QAALGLLTLCVMLLIGLVTLGIMFLQMSSE + Deamidated (NQ); Oxidation (M)
207 603.3390 602.3317 602.3275 6.96 0 4 0.93 +1Score > 46 indicates identity
Score > 16 indicates homology
AIVATE
3352 718.3887 2152.1443 2152.1391 2.42 0 4 0.69 +1Score > 37 indicates identity
Score > 15 indicates homology
SLVQVVTAWNIPMHLWLK + 2 Deamidated (NQ); Oxidation (M)
3843 820.4510 2458.3312 2458.2890 17.2 1 4 0.73 +1Score > 34 indicates identity
Score > 15 indicates homology
ELLNIYNMAVTQIPTNKPVQR + Deamidated (NQ); Oxidation (M)
3064 666.0438 1995.1096 1995.0823 13.7 0 4 0.52 +1Score > 34 indicates identity
Score > 13 indicates homology
NIVMAAALAHGVTTIINAAK + Deamidated (NQ); Oxidation (M)
3305 1068.0332 2134.0518 2134.0252 12.5 1 4 0.82 +1Score > 40 indicates identity
Score > 15 indicates homology
LDLSNNNLSGGVPEFLADMK + Deamidated (NQ)
3237 1051.0808 2100.1470 2100.1619 -7.08 0 4 1 +1Score > 35 indicates identity
Score > 16 indicates homology
LLSSQFTFLNVLTYLSVR
3550 750.0936 2247.2590 2247.2456 5.95 1 4 0.63 +1Score > 32 indicates identity
Score > 14 indicates homology
RQQLIVMLNLHRPTCIVR + Deamidated (NQ)
849 911.4845 910.4772 910.4621 16.6 0 4 0.57 +1Score > 39 indicates identity
Score > 14 indicates homology
QDVISHGR
3578 1134.0789 2266.1432 2266.1780 -15.3 1 4 0.79 +1Score > 39 indicates identity
Score > 15 indicates homology
VQLTQQFCKLFQALGLNTR + 2 Deamidated (NQ)
3159 1027.4901 2052.9656 2052.9640 0.79 0 4 0.9 +1Score > 39 indicates identity
Score > 16 indicates homology
LTQGDYSQVQLFNSDPIE
3611 1147.0809 2292.1472 2292.1745 -11.9 0 4 0.89 +1Score > 39 indicates identity
Score > 16 indicates homology
MSAVIMQQGDLLLNLYIQPK + 2 Deamidated (NQ); Oxidation (M)
2961 642.3112 1923.9118 1923.9115 0.13 0 4 0.6 +1Score > 40 indicates identity
Score > 14 indicates homology
QSIGFNWGAAGVSTSVWR + 2 Deamidated (NQ)
3209 1044.5537 2087.0928 2087.0701 10.9 1 4 0.83 +1Score > 38 indicates identity
Score > 15 indicates homology
GQLLWQIFGNQGNRWIR + 2 Deamidated (NQ)
3530 1119.5845 2237.1544 2237.1467 3.47 1 4 0.95 +1Score > 38 indicates identity
Score > 16 indicates homology
SVQGIQYLPNVTKLFLNGNK + 5 Deamidated (NQ)
3711 784.4632 2350.3678 2350.3220 19.5 0 4 1 +1Score > 27 indicates identity
Score > 16 indicates homology
SLLVINLSGNNLSGSVPQALLNK
3918 631.8047 2523.1897 2523.2099 -8.03 0 4 0.6 +1Score > 38 indicates identity
Score > 14 indicates homology
TMPFPGFPTDLQPLMMSLLATAE + Oxidation (M)
4192 703.1436 2808.5453 2808.5686 -8.29 1 4 0.94 +1Score > 32 indicates identity
Score > 16 indicates homology
LKMITYLGGILGGFIGIIQGVLAMWI + 2 Oxidation (M)
4437 1034.5170 3100.5292 3100.5508 -6.96 1 4 0.76 +1Score > 36 indicates identity
Score > 15 indicates homology
TAVNAVGNSLPLMAMPLGVQVHNIEMHPK + Deamidated (NQ); 2 Oxidation (M)
3015 984.0656 1966.1166 1966.0788 19.2 1 4 0.65 +1Score > 33 indicates identity
Score > 14 indicates homology
QYIGGIEHAILHLLYAR
4123 919.1541 2754.4405 2754.4852 -16.3 0 4 1 +1Score > 36 indicates identity
Score > 16 indicates homology
LLSFIQSIVGNWGLAIMMLTFIVR + Deamidated (NQ); 2 Oxidation (M)
2602 860.4266 1718.8386 1718.8111 16.0 1 4 0.89 +1Score > 41 indicates identity
Score > 16 indicates homology
SPTVADFSAQIRSNPE + Deamidated (NQ)
3864 825.7712 2474.2918 2474.3282 -14.7 1 4 0.86 +1Score > 37 indicates identity
Score > 16 indicates homology
RDYTIVSGGTQNHLFLVSLLNK
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Not what you expected? Try the select summary.