MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2006 719.9227 1437.8308 1437.8344 -2.45 0 5 0.93 +1Score > 35 indicates identity
Score > 17 indicates homology
SPLPIVIYNIPGR
3710 784.0594 2349.1564 2349.1257 13.0 1 5 0.89 +1Score > 39 indicates identity
Score > 17 indicates homology
ENASVSIVDDILATGGTLNAMIE + Deamidated (NQ); Oxidation (M)
3196 694.3752 2080.1038 2080.1099 -2.94 1 5 0.56 +1Score > 38 indicates identity
Score > 15 indicates homology
SLQPSVLMQLKLTDGSAHR
3216 698.3796 2092.1170 2092.1528 -17.1 1 5 0.87 +1Score > 37 indicates identity
Score > 17 indicates homology
DGLITPTIAPNGAQVLQVKR + 2 Deamidated (NQ)
2908 473.2388 1888.9261 1888.8989 14.4 0 5 0.68 +1Score > 40 indicates identity
Score > 16 indicates homology
HSAIFQSLQGNSMNPIK + 2 Deamidated (NQ); Oxidation (M)
3537 748.4169 2242.2289 2242.1917 16.6 1 5 0.7 +1Score > 35 indicates identity
Score > 16 indicates homology
TRQQIQQLDTQLLTAISQR + 2 Deamidated (NQ)
4401 1021.5285 3061.5637 3061.5204 14.1 1 5 0.44 +1Score > 36 indicates identity
Score > 14 indicates homology
GSVTAGNASGINDGAAAVLIASDEAVAQYQLK + Deamidated (NQ)
3929 848.7704 2543.2894 2543.3200 -12.0 1 5 0.74 +1Score > 38 indicates identity
Score > 16 indicates homology
LGGAMKNVLAVATGVADGMQLGLNAR + Deamidated (NQ); Oxidation (M)
4189 702.8955 2807.5529 2807.5605 -2.72 1 5 0.39 +1Score > 31 indicates identity
Score > 13 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
2891 628.3024 1881.8854 1881.8891 -1.96 1 5 0.95 +1Score > 40 indicates identity
Score > 17 indicates homology
RNLLGVQDFGSDMGSIR + 2 Deamidated (NQ); Oxidation (M)
3894 627.8328 2507.3021 2507.3287 -10.6 1 5 0.69 +1Score > 36 indicates identity
Score > 16 indicates homology
FGVFPAILSFPLDYPLSPPKMR + Oxidation (M)
3535 748.4143 2242.2211 2242.1944 11.9 1 5 0.59 +1Score > 35 indicates identity
Score > 15 indicates homology
GLDVVIVTTATNDADAKALLLE + Deamidated (NQ)
3480 737.0651 2208.1735 2208.1348 17.5 1 5 0.52 +1Score > 37 indicates identity
Score > 15 indicates homology
FAQTLTAGMQILEQDLATLK + Deamidated (NQ); Oxidation (M)
4053 895.4588 2683.3546 2683.3666 -4.49 0 5 0.86 +1Score > 37 indicates identity
Score > 17 indicates homology
LSSSDNVPYMVTLLSVINAIIFGTE + Deamidated (NQ)
3361 719.0745 2154.2017 2154.2194 -8.24 1 5 0.59 +1Score > 33 indicates identity
Score > 15 indicates homology
RVSNLVPMTIIGAASNLIIR + Deamidated (NQ); Oxidation (M)
4268 965.1920 2892.5542 2892.5273 9.29 0 5 0.41 +1Score > 33 indicates identity
Score > 14 indicates homology
VQIQDNLFLIPVPQSDIRPVAWLTE + 2 Deamidated (NQ)
4625 849.9311 3395.6953 3395.6721 6.84 1 5 0.72 +1Score > 35 indicates identity
Score > 16 indicates homology
YDVSGIQIHDMGLAKYINLNSYFNLHTGGR
3123 679.3626 2035.0660 2035.0448 10.4 1 5 1 +1Score > 38 indicates identity
Score > 17 indicates homology
YWTLVNILSTPMTPEVR + Oxidation (M)
3195 693.7099 2078.1079 2078.0830 12.0 1 5 0.93 +1Score > 37 indicates identity
Score > 17 indicates homology
RSQPQLTQMSVPLVHQVK + 3 Deamidated (NQ)
260 317.1940 632.3734 632.3857 -19.4 1 5 0.98 +1Score > 36 indicates identity
Score > 17 indicates homology
VTTKGK
1606 414.5582 1240.6528 1240.6775 -19.9 1 5 0.91 +1Score > 40 indicates identity
Score > 17 indicates homology
LEVANDALGALR
2887 627.6633 1879.9681 1879.9792 -5.92 1 5 0.72 +1Score > 39 indicates identity
Score > 16 indicates homology
KGSTNVTVSYNYIHGIK
2947 639.9849 1916.9329 1916.9340 -0.59 1 5 0.99 +1Score > 40 indicates identity
Score > 17 indicates homology
QQNLQQRQQQPPPPAR + 4 Deamidated (NQ)
1457 591.3682 1180.7218 1180.7067 12.8 1 5 0.73 +1Score > 31 indicates identity
Score > 16 indicates homology
DVVPVGILLKE
3797 806.7504 2417.2294 2417.2438 -5.96 1 5 0.63 +1Score > 38 indicates identity
Score > 15 indicates homology
SVNNPVTIKFNLGSAPSQATTLR + 3 Deamidated (NQ)
4396 611.5251 3052.5891 3052.5283 19.9 1 5 0.52 +1Score > 35 indicates identity
Score > 14 indicates homology
MKCAVNVQPVTIINLDGTNIMNFVSIK + 2 Deamidated (NQ); 2 Oxidation (M)
3682 777.4201 2329.2385 2329.2060 14.0 1 5 0.58 +1Score > 36 indicates identity
Score > 15 indicates homology
RQGLGMQVSALVNDTVATLAGAR + 2 Deamidated (NQ)
4292 728.3812 2909.4957 2909.4845 3.85 0 5 1 +1Score > 36 indicates identity
Score > 17 indicates homology
QVTAPTVVIVVNNNGGQIFSMLPTPPAE + Deamidated (NQ); Oxidation (M)
4922 813.4070 4874.3983 4874.3129 17.5 1 5 1 +1Score > 30 indicates identity
Score > 17 indicates homology
FRVDMPGSGSAFIPTINAITTTSQDLQWMVQPTVITSMSNPYPR + 4 Deamidated (NQ); Oxidation (M)
4131 690.6353 2758.5121 2758.4709 14.9 0 5 0.8 +1Score > 32 indicates identity
Score > 16 indicates homology
GVFQLSYLVNFWWVGCILAFLVK
3470 735.3596 2203.0570 2203.0566 0.17 1 5 0.64 +1Score > 39 indicates identity
Score > 15 indicates homology
EGVSGQVLVDSNDLLTMPLSE + Deamidated (NQ)
4580 662.5701 3307.8141 3307.7964 5.35 1 5 0.43 +1Score > 30 indicates identity
Score > 14 indicates homology
VLYILLLVVGFSLMCLELFHSSNVIDGLK + Oxidation (M)
4197 939.1820 2814.5242 2814.5396 -5.49 1 5 0.5 +1Score > 32 indicates identity
Score > 14 indicates homology
MRTLGAMAIMLVVMGTVIFLSFILR + 2 Oxidation (M)
3293 710.3742 2128.1008 2128.1067 -2.80 0 5 0.76 +1Score > 38 indicates identity
Score > 16 indicates homology
FWLLTAIACGLGVVLFYGE
4150 925.8429 2774.5069 2774.4525 19.6 1 5 0.59 +1Score > 33 indicates identity
Score > 15 indicates homology
VMTGFRVGPQGVQGLTGITPDLTTLAK + 2 Deamidated (NQ); Oxidation (M)
2122 750.4446 1498.8746 1498.8871 -8.34 1 5 0.76 +1Score > 33 indicates identity
Score > 16 indicates homology
KIAGVGVDIVYLPR
2521 558.2861 1671.8365 1671.8250 6.86 0 5 0.61 +1Score > 40 indicates identity
Score > 15 indicates homology
HVTNAANNTTIQIMK + Deamidated (NQ); Oxidation (M)
4068 898.4672 2692.3798 2692.3391 15.1 1 5 0.6 +1Score > 37 indicates identity
Score > 15 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
2927 636.6176 1906.8310 1906.8328 -0.97 0 5 0.61 +1Score > 37 indicates identity
Score > 15 indicates homology
TLQDMMNIIDYNQYK + 2 Deamidated (NQ); Oxidation (M)
3287 1064.5565 2127.0984 2127.1180 -9.20 0 5 0.56 +1Score > 38 indicates identity
Score > 15 indicates homology
AAGVINNVVASVPMVVNAVMR + Deamidated (NQ); Oxidation (M)
3529 1119.5836 2237.1526 2237.1467 2.67 1 5 0.65 +1Score > 38 indicates identity
Score > 15 indicates homology
SVQGIQYLPNVTKLFLNGNK + 5 Deamidated (NQ)
1163 346.8705 1037.5897 1037.5869 2.63 0 5 0.88 +1Score > 36 indicates identity
Score > 17 indicates homology
QLLSVDVHK
4166 698.3520 2789.3789 2789.3429 12.9 0 5 0.42 +1Score > 37 indicates identity
Score > 13 indicates homology
QINLDSDLLGDARPFLQDGMTVQLE + 2 Deamidated (NQ)
1803 671.9147 1341.8148 1341.8232 -6.19 1 5 0.63 +1Score > 31 indicates identity
Score > 15 indicates homology
LSLKQTDVVIVK
3105 506.2701 2021.0513 2021.0364 7.38 1 5 0.48 +1Score > 38 indicates identity
Score > 14 indicates homology
IAAASGSPMPKLPSDSALHR + Oxidation (M)
3434 1097.0347 2192.0548 2192.0460 4.06 1 5 0.86 +1Score > 39 indicates identity
Score > 16 indicates homology
AEQPQFTPHIPAMGLPVTQE + 2 Deamidated (NQ)
2353 535.2728 1602.7966 1602.7810 9.70 1 5 0.7 +1Score > 41 indicates identity
Score > 16 indicates homology
NSILANSIPENMGIK + 3 Deamidated (NQ)
4689 894.4963 3573.9561 3573.9554 0.19 1 5 0.82 +1Score > 29 indicates identity
Score > 16 indicates homology
LVFGVTLLQGAMLTGVATFLILMLQNRGQKPLE + 3 Deamidated (NQ)
2847 620.3226 1857.9460 1857.9329 7.06 1 5 0.6 +1Score > 39 indicates identity
Score > 15 indicates homology
MQLHMGLSKTDGVVTPK + Deamidated (NQ); Oxidation (M)
3357 718.7226 2153.1460 2153.1633 -8.04 0 5 0.77 +1Score > 37 indicates identity
Score > 16 indicates homology
RPQIQTIAPGAQIAHFLYK + 2 Deamidated (NQ)
4595 1114.6107 3340.8103 3340.7749 10.6 1 5 0.44 +1Score > 31 indicates identity
Score > 14 indicates homology
ALMGMKTDAPAVTAFTAVIQMGAILAAIVYFR
2751 901.4973 1800.9800 1800.9655 8.06 1 5 0.87 +1Score > 37 indicates identity
Score > 16 indicates homology
LVVLGGAMGATTLNAEGVK + Deamidated (NQ)
2773 906.5021 1810.9896 1810.9577 17.6 0 5 0.9 +1Score > 37 indicates identity
Score > 17 indicates homology
GKPGVNALQDIDFAIPR + Deamidated (NQ)
3364 720.0564 2157.1474 2157.1357 5.39 1 5 0.81 +1Score > 37 indicates identity
Score > 16 indicates homology
KVDYLVLGGAIANNFLSFSK + 2 Deamidated (NQ)
3513 744.3936 2230.1590 2230.1481 4.85 0 5 0.76 +1Score > 38 indicates identity
Score > 16 indicates homology
SGVGQLTIIDFDLVEPGNTVR + Deamidated (NQ)
3886 835.0848 2502.2326 2502.2166 6.38 1 5 0.86 +1Score > 38 indicates identity
Score > 16 indicates homology
FDAVVELDASQILPQVTWGTSPE + Deamidated (NQ)
2549 563.3021 1686.8845 1686.9039 -11.5 0 5 0.81 +1Score > 40 indicates identity
Score > 16 indicates homology
TTLNIQAIQQDSLIK + 2 Deamidated (NQ)
3048 662.0558 1983.1456 1983.1193 13.2 1 5 1 +1Score > 30 indicates identity
Score > 17 indicates homology
VPWGIVLLLGGGFALAKGSE
3874 830.7647 2489.2723 2489.2480 9.74 1 5 0.94 +1Score > 37 indicates identity
Score > 17 indicates homology
LPGNGWAMQLLAVTCLSLAAKME + Oxidation (M)
4066 898.4567 2692.3483 2692.3391 3.40 1 5 0.49 +1Score > 37 indicates identity
Score > 14 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
2405 539.6307 1615.8703 1615.8458 15.2 1 5 0.56 +1Score > 39 indicates identity
Score > 15 indicates homology
VVIGGLEDFVVGNIGE
3269 1059.5582 2117.1018 2117.1303 -13.5 1 5 0.61 +1Score > 38 indicates identity
Score > 15 indicates homology
TVAFVGPSGAGKSTLCSLLPR
3628 576.0702 2300.2517 2300.2164 15.3 1 5 0.59 +1Score > 35 indicates identity
Score > 15 indicates homology
NRPVDVLQWIDLHINPKLE + 2 Deamidated (NQ)
2713 889.4725 1776.9304 1776.9192 6.31 1 5 0.83 +1Score > 39 indicates identity
Score > 16 indicates homology
NLTRSVVPAIDLYCR + Deamidated (NQ)
3454 733.0233 2196.0481 2196.0442 1.76 1 5 0.64 +1Score > 39 indicates identity
Score > 15 indicates homology
TATIDKNNMMQYIAPQDLK + 2 Deamidated (NQ)
4098 908.8068 2723.3986 2723.3840 5.37 0 5 0.97 +1Score > 36 indicates identity
Score > 17 indicates homology
AIGIQQNSIYMVEPNLNITNIYSK + Deamidated (NQ)
4155 696.3486 2781.3653 2781.3241 14.8 1 5 0.91 +1Score > 37 indicates identity
Score > 17 indicates homology
QFQMATQLVTMVLKIDNVVSDFHD + 3 Deamidated (NQ)
4591 1112.5890 3334.7452 3334.7382 2.09 1 5 1 +1Score > 33 indicates identity
Score > 17 indicates homology
NLIAAHSHIRGLGVDVDSLQPRPASQGLVGQE + Deamidated (NQ)
4125 689.6212 2754.4557 2754.4449 3.93 0 4 0.66 +1Score > 35 indicates identity
Score > 15 indicates homology
GTYLVTVILLVSTLSVGMCSNGWIR + Oxidation (M)
2372 806.4354 1610.8562 1610.8403 9.90 0 4 0.81 +1Score > 39 indicates identity
Score > 16 indicates homology
SVQQQSTPVPLISPK + 3 Deamidated (NQ)
3045 991.9944 1981.9742 1982.0044 -15.2 1 4 0.77 +1Score > 40 indicates identity
Score > 16 indicates homology
GGGVNHPLAGMLYGGAIIER + Deamidated (NQ)
3751 595.7913 2379.1361 2379.0974 16.3 0 4 0.6 +1Score > 39 indicates identity
Score > 15 indicates homology
SLSVINITYTYNGSAGPVSMCK + 2 Deamidated (NQ); Oxidation (M)
4089 903.8225 2708.4457 2708.4836 -14.0 1 4 0.64 +1Score > 34 indicates identity
Score > 15 indicates homology
RLLAYSSIAHMGYLLTGFVAAGLLR + Oxidation (M)
1568 410.5815 1228.7227 1228.7292 -5.29 1 4 0.84 +1Score > 36 indicates identity
Score > 16 indicates homology
RIFPAILSGQK
2437 327.3993 1631.9601 1631.9583 1.10 1 4 0.75 +1Score > 30 indicates identity
Score > 16 indicates homology
LVQQRPALPDLARR
4594 836.2074 3340.8005 3340.7662 10.3 0 4 0.54 +1Score > 32 indicates identity
Score > 14 indicates homology
TSPTALIVVGVLNACSAGLLIYMALVNLLAHE + 2 Deamidated (NQ); Oxidation (M)
3636 769.0666 2304.1780 2304.2069 -12.5 1 4 0.58 +1Score > 38 indicates identity
Score > 15 indicates homology
QADLIITAGTITMKMAPQLVR + 2 Deamidated (NQ); 2 Oxidation (M)
640 411.7327 821.4508 821.4582 -8.90 1 4 0.86 +1Score > 39 indicates identity
Score > 16 indicates homology
FKPKMR + Oxidation (M)
2711 593.0054 1775.9944 1775.9716 12.8 1 4 0.66 +1Score > 35 indicates identity
Score > 15 indicates homology
GIAAVGAPVVVEALAMHR + Oxidation (M)
3685 777.7278 2330.1616 2330.1349 11.4 1 4 1 +1Score > 39 indicates identity
Score > 17 indicates homology
ERPLLNANTTQNGVSQTTINR + 4 Deamidated (NQ)
4063 898.4468 2692.3186 2692.2915 10.0 0 4 0.84 +1Score > 38 indicates identity
Score > 16 indicates homology
GPPGPAGPAGLMGPPGLQGPSGLPGDPGDR + Deamidated (NQ); Oxidation (M)
4746 942.4952 3765.9517 3765.9173 9.12 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
GSFSGSAGINSLAGSANLRTLGVDDVVQGNNTYGLLLK + Deamidated (NQ)
4262 722.8749 2887.4705 2887.4745 -1.38 0 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
MISLTNPDVVILSGDDALTLPMMAVGAK + Oxidation (M)
3418 1093.5983 2185.1820 2185.1630 8.71 1 4 0.58 +1Score > 36 indicates identity
Score > 15 indicates homology
LTELPNLLPNLANVHLDAVE + Deamidated (NQ)
3491 739.7029 2216.0869 2216.0961 -4.14 1 4 0.91 +1Score > 39 indicates identity
Score > 16 indicates homology
LALADNVPYNVVDSQNLKNE + Deamidated (NQ)
144 516.3156 515.3083 515.3179 -18.7 1 4 0.62 +1Score > 47 indicates identity
Score > 15 indicates homology
AAKAR
2721 595.6747 1784.0023 1783.9794 12.8 1 4 0.87 +1Score > 35 indicates identity
Score > 16 indicates homology
KPELNIVQPMIPLFK + 2 Deamidated (NQ); Oxidation (M)
3473 736.0518 2205.1336 2205.1616 -12.7 0 4 0.98 +1Score > 38 indicates identity
Score > 17 indicates homology
FLTIVTLSTHPPQICHGVGK + Deamidated (NQ)
1339 378.8710 1133.5912 1133.5717 17.2 1 4 0.87 +1Score > 42 indicates identity
Score > 16 indicates homology
QAVEAAFDGVK
3481 737.0679 2208.1819 2208.1388 19.5 0 4 0.63 +1Score > 36 indicates identity
Score > 15 indicates homology
ADTLVIAAPMYNFTIPTQLK + 2 Deamidated (NQ)
3706 1175.0608 2348.1070 2348.1028 1.82 0 4 0.79 +1Score > 38 indicates identity
Score > 16 indicates homology
QNLIQNPMGTDNLLNCLYPK + 3 Deamidated (NQ)
4190 702.8964 2807.5565 2807.5605 -1.44 1 4 0.45 +1Score > 31 indicates identity
Score > 13 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
3577 1134.0786 2266.1426 2266.1007 18.5 1 4 0.89 +1Score > 39 indicates identity
Score > 16 indicates homology
TNIVSTGQALMVLIMGGQMER + 2 Deamidated (NQ); Oxidation (M)
4065 898.4561 2692.3465 2692.3391 2.73 1 4 0.55 +1Score > 37 indicates identity
Score > 14 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
3892 836.4235 2506.2487 2506.2083 16.1 1 4 1 +1Score > 38 indicates identity
Score > 17 indicates homology
NVALPIGQGQTISQPYMVARMTE + 3 Deamidated (NQ)
3580 756.3906 2266.1500 2266.1118 16.9 1 4 0.98 +1Score > 38 indicates identity
Score > 17 indicates homology
AGATTVNIPDTVGYSTPLEFGR + Deamidated (NQ)
4187 702.8946 2807.5493 2807.5605 -4.00 1 4 0.47 +1Score > 31 indicates identity
Score > 14 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4194 937.4844 2809.4314 2809.3931 13.6 1 4 0.71 +1Score > 36 indicates identity
Score > 15 indicates homology
SLVFRPPNPWTMAIMNVLAELHQE + Deamidated (NQ); Oxidation (M)
2191 765.9240 1529.8334 1529.8388 -3.49 1 4 0.83 +1Score > 39 indicates identity
Score > 16 indicates homology
QMFKIPIQAAIGGR + Deamidated (NQ)
4184 702.8940 2807.5469 2807.5605 -4.86 1 4 0.73 +1Score > 31 indicates identity
Score > 15 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
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