MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3464 734.0679 2199.1819 2199.2007 -8.55 1 6 0.68 +1Score > 36 indicates identity
Score > 17 indicates homology
LLKPTVMMNLLGRDLDLLE + Oxidation (M)
270 643.3663 642.3590 642.3588 0.31 0 6 0.43 +1Score > 35 indicates identity
Score > 15 indicates homology
AIPITE
1896 695.3962 1388.7778 1388.7565 15.4 1 6 0.75 +1Score > 37 indicates identity
Score > 18 indicates homology
HPVSPWGVPTKGK
3093 504.7467 2014.9577 2014.9744 -8.27 1 6 0.34 +1Score > 39 indicates identity
Score > 14 indicates homology
MGNGMNKILPSLFIGNFK + 3 Deamidated (NQ); 2 Oxidation (M)
2791 911.9164 1821.8182 1821.7985 10.8 1 6 0.83 +1Score > 38 indicates identity
Score > 18 indicates homology
NAKLDMNGNCLNAVDR + 2 Deamidated (NQ); Oxidation (M)
3133 680.3428 2038.0066 2038.0445 -18.6 0 6 0.87 +1Score > 40 indicates identity
Score > 18 indicates homology
CIGTSFPNFTALLQGVTVI + Deamidated (NQ)
3412 728.0593 2181.1561 2181.1173 17.8 0 6 0.57 +1Score > 37 indicates identity
Score > 16 indicates homology
AMGADAILLILAMLDHGQAIE + Oxidation (M)
4502 796.4291 3181.6873 3181.7057 -5.78 1 6 1 +1Score > 33 indicates identity
Score > 19 indicates homology
NLQTGTLIIGASQTTGTYLMPQLISLFRK + Deamidated (NQ); Oxidation (M)
4776 970.9888 3879.9261 3879.9254 0.18 1 6 0.73 +1Score > 34 indicates identity
Score > 17 indicates homology
SFVTQHAAFGYMALDYGLNQISINGVIPDAEPSAKR
3515 1116.5479 2231.0812 2231.1070 -11.5 1 6 0.36 +1Score > 39 indicates identity
Score > 14 indicates homology
NQLQQQGIILEDSATGTTWK + Deamidated (NQ)
3587 758.4109 2272.2109 2272.1997 4.90 1 6 0.95 +1Score > 36 indicates identity
Score > 19 indicates homology
KSNFSLAILNVGAPAAGMNAAVR + Deamidated (NQ)
3917 841.7891 2522.3455 2522.3090 14.4 1 6 0.61 +1Score > 35 indicates identity
Score > 17 indicates homology
QFVLGNLDVTIIAQAPKMAPHIE + 2 Deamidated (NQ); Oxidation (M)
3939 851.4805 2551.4197 2551.3857 13.3 1 6 1 +1Score > 32 indicates identity
Score > 19 indicates homology
GLNVLDVINNDKLVITQGALSQVE
3337 716.3826 2146.1260 2146.0906 16.5 1 6 0.67 +1Score > 38 indicates identity
Score > 17 indicates homology
DEKPINFDNALLSVTSLNR + Deamidated (NQ)
4136 922.8461 2765.5165 2765.4633 19.2 1 6 0.38 +1Score > 32 indicates identity
Score > 14 indicates homology
NMIVSRYSLVQTNALASLVNLSLDK + Deamidated (NQ); Oxidation (M)
2183 509.9659 1526.8759 1526.8609 9.79 1 6 0.54 +1Score > 35 indicates identity
Score > 16 indicates homology
FVHPFVLDKLNAK
3428 1095.0720 2188.1294 2188.1375 -3.69 1 6 0.72 +1Score > 38 indicates identity
Score > 17 indicates homology
VKNGNGATAEPSLNILAQYVK + 2 Deamidated (NQ)
1238 543.2849 1084.5552 1084.5513 3.67 0 6 0.98 +1Score > 40 indicates identity
Score > 19 indicates homology
LQISDPNGNK
2893 471.7312 1882.8957 1882.8818 7.38 0 6 0.75 +1Score > 40 indicates identity
Score > 17 indicates homology
LCSLNINMHHDPFLR + Deamidated (NQ); Oxidation (M)
3321 713.0329 2136.0769 2136.0838 -3.24 1 6 0.33 +1Score > 39 indicates identity
Score > 14 indicates homology
TIIINGVQFNTDEDTTILK + 2 Deamidated (NQ)
3576 756.0577 2265.1513 2265.1463 2.17 0 6 0.93 +1Score > 39 indicates identity
Score > 18 indicates homology
LPPPYTAIASPDAGGVPVINCR + Deamidated (NQ)
1287 557.2616 1112.5086 1112.5172 -7.67 1 6 0.88 +1Score > 39 indicates identity
Score > 18 indicates homology
ELMDYAISR + Oxidation (M)
3924 845.4898 2533.4476 2533.4681 -8.10 1 6 0.44 +1Score > 29 indicates identity
Score > 15 indicates homology
WPPFLLGCTILVILLVMKHVGK
4460 784.3986 3133.5653 3133.5028 19.9 1 6 0.38 +1Score > 36 indicates identity
Score > 15 indicates homology
FEVVLYLNQMGVFNFVDDGSLPGCAVLK + 3 Deamidated (NQ)
2928 636.6610 1906.9612 1906.9537 3.91 1 6 0.69 +1Score > 40 indicates identity
Score > 17 indicates homology
VHFVANIDGAEIHDLTR + Deamidated (NQ)
3292 1064.6185 2127.2224 2127.2263 -1.81 1 6 0.35 +1Score > 29 indicates identity
Score > 14 indicates homology
NLVTDANRSIATLAITTLLK
3413 728.0603 2181.1591 2181.1641 -2.30 1 6 0.4 +1Score > 37 indicates identity
Score > 15 indicates homology
ATTPLTAATSNPSTPAKPAKQK + Deamidated (NQ)
3445 1097.5170 2193.0194 2192.9783 18.7 1 6 0.59 +1Score > 39 indicates identity
Score > 16 indicates homology
DLSHAKIFITIMGQDDDQE + 2 Deamidated (NQ); Oxidation (M)
4426 1029.9055 3086.6947 3086.7137 -6.15 1 6 0.53 +1Score > 31 indicates identity
Score > 16 indicates homology
QATLMVALGGAIGSVARYWLALLMLPISR + Oxidation (M)
2460 411.9756 1643.8733 1643.8770 -2.24 0 6 0.52 +1Score > 40 indicates identity
Score > 16 indicates homology
PALSLQQLQLINFR + 4 Deamidated (NQ)
3362 1079.5641 2157.1136 2157.0888 11.5 1 6 0.53 +1Score > 38 indicates identity
Score > 16 indicates homology
VSGSVAAPTASLHFTPELMAR + Oxidation (M)
2339 533.2811 1596.8215 1596.8107 6.73 1 6 0.69 +1Score > 40 indicates identity
Score > 17 indicates homology
KAQVNQSTLSTYTR + Deamidated (NQ)
2719 594.6810 1781.0212 1781.0159 2.94 1 6 1 +1Score > 32 indicates identity
Score > 19 indicates homology
RLGLGLQGSVQVLPSTR + Deamidated (NQ)
2979 645.6736 1933.9990 1934.0261 -14.0 0 6 0.96 +1Score > 39 indicates identity
Score > 18 indicates homology
ADLPLLSHNLPADFAALR + Deamidated (NQ)
1664 426.5647 1276.6723 1276.6663 4.67 1 6 0.9 +1Score > 41 indicates identity
Score > 18 indicates homology
SPLFAGAKVASTE
3708 784.0535 2349.1387 2349.1392 -0.23 1 6 0.56 +1Score > 39 indicates identity
Score > 16 indicates homology
VMGMRHFDVQLIGGMVLDSGR + 2 Oxidation (M)
3718 590.3216 2357.2573 2357.2155 17.8 0 6 0.29 +1Score > 36 indicates identity
Score > 13 indicates homology
NADGIQLGPFDLSQPYIAPIVK + 2 Deamidated (NQ)
3063 665.0078 1992.0016 1992.0356 -17.1 1 6 0.84 +1Score > 40 indicates identity
Score > 18 indicates homology
LRLIWSPSPYATAQLFE + Deamidated (NQ)
3328 715.3688 2143.0846 2143.1096 -11.7 1 6 0.68 +1Score > 39 indicates identity
Score > 17 indicates homology
LKFVGTATAGMDHVDQALLR + Deamidated (NQ)
3499 1112.0245 2222.0344 2222.0572 -10.3 1 6 0.78 +1Score > 39 indicates identity
Score > 17 indicates homology
DQQAMVKVPNTFSVCTNQR
2371 806.4218 1610.8290 1610.8264 1.64 0 6 1 +1Score > 40 indicates identity
Score > 19 indicates homology
TDPGVSAVGNAVLAPSR + Deamidated (NQ)
3713 786.4224 2356.2454 2356.2095 15.2 1 6 0.64 +1Score > 37 indicates identity
Score > 17 indicates homology
VNAQTGKLSQVSGLGQHTTTSSR
641 823.4047 822.3974 822.4123 -18.1 1 6 0.83 +1Score > 42 indicates identity
Score > 18 indicates homology
TQLFGEK + Deamidated (NQ)
3922 633.8289 2531.2865 2531.2981 -4.60 0 6 0.83 +1Score > 38 indicates identity
Score > 18 indicates homology
DMSALFALSNVAYGLSIIDLFQK + Oxidation (M)
1847 455.2309 1362.6709 1362.6449 19.1 1 6 0.84 +1Score > 41 indicates identity
Score > 18 indicates homology
ILAQAMQRQASE + 2 Deamidated (NQ); Oxidation (M)
2926 953.5316 1905.0486 1905.0758 -14.2 1 6 0.88 +1Score > 35 indicates identity
Score > 18 indicates homology
QKTVIPGMPTVIPPGLTR + Deamidated (NQ)
3750 794.0518 2379.1336 2379.1304 1.33 1 6 0.9 +1Score > 39 indicates identity
Score > 18 indicates homology
FASQLNFTIVPETLAAMTSHAE + 2 Deamidated (NQ)
265 318.6906 635.3666 635.3676 -1.52 1 6 0.85 +1Score > 39 indicates identity
Score > 18 indicates homology
TMKIK + Oxidation (M)
2168 759.3602 1516.7058 1516.7256 -13.1 1 6 0.54 +1Score > 40 indicates identity
Score > 16 indicates homology
ITITNDQNRLTPE + 3 Deamidated (NQ)
2953 480.7666 1919.0373 1919.0629 -13.3 1 6 0.52 +1Score > 37 indicates identity
Score > 16 indicates homology
SYGIHVAELAGIPQPVIR
2345 799.8807 1597.7468 1597.7770 -18.9 1 6 0.68 +1Score > 40 indicates identity
Score > 17 indicates homology
VDPGDNVIAKGEPCK
2384 538.3235 1611.9487 1611.9308 11.1 1 6 0.95 +1Score > 32 indicates identity
Score > 18 indicates homology
SGQVLNVVNSILNKK
2889 627.9629 1880.8669 1880.8495 9.21 1 6 0.69 +1Score > 39 indicates identity
Score > 17 indicates homology
TMTAQIADALTEAMEPR + Deamidated (NQ); 2 Oxidation (M)
3916 841.7889 2522.3449 2522.3090 14.2 1 6 0.7 +1Score > 35 indicates identity
Score > 17 indicates homology
QFVLGNLDVTIIAQAPKMAPHIE + 2 Deamidated (NQ); Oxidation (M)
2533 558.9867 1673.9383 1673.9101 16.9 1 6 0.61 +1Score > 35 indicates identity
Score > 16 indicates homology
VKTSVGVWGALLNSSR + Deamidated (NQ)
4181 936.8443 2807.5111 2807.4720 13.9 1 6 1 +1Score > 33 indicates identity
Score > 18 indicates homology
FYNLMIENVFLLILQTIWQHQK + Deamidated (NQ); Oxidation (M)
3562 564.0350 2252.1109 2252.1446 -15.0 0 6 0.75 +1Score > 39 indicates identity
Score > 17 indicates homology
DIVMPGHIFPLMAQDGGVLAR + Oxidation (M)
2629 865.4798 1728.9450 1728.9145 17.7 0 6 0.5 +1Score > 38 indicates identity
Score > 15 indicates homology
ALGDILVVGLNSDNSIK + 2 Deamidated (NQ)
4482 1055.5155 3163.5247 3163.5282 -1.12 1 6 0.38 +1Score > 36 indicates identity
Score > 14 indicates homology
LAATFQSDIPSKQNQAQNGNQSHLPPQSR + 2 Deamidated (NQ)
1771 442.8990 1325.6752 1325.6589 12.3 1 6 0.8 +1Score > 41 indicates identity
Score > 17 indicates homology
QHYNAVPQRGR + Deamidated (NQ)
2779 605.6296 1813.8670 1813.8482 10.3 1 6 0.99 +1Score > 40 indicates identity
Score > 18 indicates homology
QNNGFAFGLSSKITGDR + 3 Deamidated (NQ)
2603 860.4274 1718.8402 1718.8661 -15.1 0 6 0.7 +1Score > 41 indicates identity
Score > 17 indicates homology
GQTAPLYLASDPLAMR + Oxidation (M)
4002 875.4280 2623.2622 2623.3017 -15.1 1 6 0.47 +1Score > 38 indicates identity
Score > 15 indicates homology
VDALQSQTDVESFQLLLGLADGFR + 2 Deamidated (NQ)
4419 771.1421 3080.5393 3080.5284 3.55 0 6 0.4 +1Score > 36 indicates identity
Score > 14 indicates homology
VLYGQIVDTNAAWLADFFFHQGLPLSR + 3 Deamidated (NQ)
835 903.4821 902.4748 902.4709 4.36 1 6 0.85 +1Score > 43 indicates identity
Score > 18 indicates homology
KVNNLQSV + 2 Deamidated (NQ)
2493 834.4484 1666.8822 1666.8890 -4.05 1 6 0.85 +1Score > 39 indicates identity
Score > 18 indicates homology
QQEQVLTVALGQPVR + 2 Deamidated (NQ)
2962 962.9711 1923.9276 1923.9301 -1.28 1 6 0.83 +1Score > 40 indicates identity
Score > 18 indicates homology
LDAPYQAGMRNFNWIK + Deamidated (NQ)
2551 846.3795 1690.7444 1690.7475 -1.78 1 6 0.76 +1Score > 38 indicates identity
Score > 17 indicates homology
QLAEYDIYAGQPHGE + Deamidated (NQ)
2880 627.0082 1878.0028 1877.9669 19.1 0 6 0.81 +1Score > 37 indicates identity
Score > 17 indicates homology
IGNIGAGNAATAISYMINK
2558 566.2940 1695.8602 1695.8792 -11.2 0 6 0.85 +1Score > 40 indicates identity
Score > 18 indicates homology
VDSVVPLNNIQDNLR + Deamidated (NQ)
3489 738.0984 2211.2734 2211.2488 11.1 1 6 1 +1Score > 29 indicates identity
Score > 18 indicates homology
SLHSPRVQATVPQPSLIRPK + Deamidated (NQ)
1875 461.9233 1382.7481 1382.7452 2.05 1 6 0.83 +1Score > 39 indicates identity
Score > 18 indicates homology
LSLMNQRHVGTK
2982 646.0247 1935.0523 1935.0248 14.2 0 6 0.47 +1Score > 36 indicates identity
Score > 15 indicates homology
AAGQTVVAQPVHMQQLLK + Deamidated (NQ); Oxidation (M)
3679 776.4150 2326.2232 2326.1838 16.9 1 6 0.65 +1Score > 37 indicates identity
Score > 17 indicates homology
MDATAAITGNVDKTQIPPLNQK + Deamidated (NQ)
3945 852.4535 2554.3387 2554.2955 16.9 1 6 1 +1Score > 36 indicates identity
Score > 18 indicates homology
LGYPGGPVIDKLAQTGNPQAFALPE + 2 Deamidated (NQ)
4196 938.1484 2811.4234 2811.3774 16.4 1 6 1 +1Score > 37 indicates identity
Score > 18 indicates homology
QASVSITPAGQLLVTVRAPASDGQANQE + 4 Deamidated (NQ)
2502 556.9822 1667.9248 1667.8995 15.2 1 6 0.8 +1Score > 36 indicates identity
Score > 17 indicates homology
EPVADLHIRYSQLK
3469 1102.5308 2203.0470 2203.0806 -15.2 1 6 0.4 +1Score > 39 indicates identity
Score > 14 indicates homology
RVLWLCLMQTYFTASVSE
3715 786.4247 2356.2523 2356.2195 13.9 1 6 0.41 +1Score > 36 indicates identity
Score > 14 indicates homology
LPIIALLATQAQGTTCIKDAQE + 2 Deamidated (NQ)
1956 472.9410 1415.8012 1415.7885 8.96 1 6 0.86 +1Score > 37 indicates identity
Score > 18 indicates homology
RNITVFQPATLR + Deamidated (NQ)
2845 929.9772 1857.9398 1857.9553 -8.30 0 6 0.98 +1Score > 40 indicates identity
Score > 18 indicates homology
QLIWQFIYQLLTYE + Deamidated (NQ)
3694 780.3998 2338.1776 2338.1765 0.48 0 6 0.81 +1Score > 38 indicates identity
Score > 17 indicates homology
NGVVTSTTALVNGAAIDHAAQLGR + 3 Deamidated (NQ)
3286 709.7241 2126.1505 2126.1591 -4.08 1 6 0.77 +1Score > 36 indicates identity
Score > 17 indicates homology
MLTAEMVKPGAIVIDVGINR
4618 1126.5702 3376.6888 3376.6366 15.4 1 6 0.94 +1Score > 35 indicates identity
Score > 18 indicates homology
AGNGAVSAAQFGSASILTISWMYIAMMGGRGLR + Deamidated (NQ); 2 Oxidation (M)
2212 514.9576 1541.8510 1541.8301 13.5 1 6 0.67 +1Score > 38 indicates identity
Score > 16 indicates homology
IVLVDEVLTPDSSR
2282 524.6437 1570.9093 1570.8831 16.6 0 6 0.64 +1Score > 33 indicates identity
Score > 16 indicates homology
NLLAGGLGIDNLFVR
3390 725.3637 2173.0693 2173.0579 5.23 1 6 0.83 +1Score > 39 indicates identity
Score > 17 indicates homology
KFLYDLNVDDGLAAVGYTAE
2787 606.6666 1816.9780 1816.9458 17.7 0 6 0.91 +1Score > 38 indicates identity
Score > 18 indicates homology
NLGAADLVILTPIYSAGE + Deamidated (NQ)
2599 858.4877 1714.9608 1714.9465 8.38 1 6 0.5 +1Score > 35 indicates identity
Score > 15 indicates homology
LSADVINLDIAKSSAAK
3310 534.7578 2135.0021 2134.9623 18.6 0 6 0.64 +1Score > 39 indicates identity
Score > 16 indicates homology
VQCNAVGCITNLATQDQNK + 2 Deamidated (NQ)
3407 728.0405 2181.0997 2181.1277 -12.9 1 6 0.59 +1Score > 39 indicates identity
Score > 16 indicates homology
LTNLGLVTVSLASGLGQHRDE + 2 Deamidated (NQ)
2338 532.9445 1595.8117 1595.8116 0.026 1 6 0.95 +1Score > 40 indicates identity
Score > 18 indicates homology
LLGQIMLDTAEYTK + Deamidated (NQ)
3846 616.3380 2461.3229 2461.3176 2.15 1 6 0.31 +1Score > 35 indicates identity
Score > 13 indicates homology
ADPSLNNPVVIQATRLDASILPR + 2 Deamidated (NQ)
3119 1018.0372 2034.0598 2034.0303 14.5 1 6 0.71 +1Score > 38 indicates identity
Score > 17 indicates homology
TMLNLNQSIQRAITSVNK + 4 Deamidated (NQ)
3152 682.7086 2045.1040 2045.0932 5.26 1 6 0.55 +1Score > 36 indicates identity
Score > 16 indicates homology
TEDLPLSAIAIDSIYTPVK
4074 900.8712 2699.5918 2699.5486 16.0 1 6 0.35 +1Score > 24 indicates identity
Score > 14 indicates homology
HALDLGISVFAIIVVTLIRHLNQR + 2 Deamidated (NQ)
2189 765.4370 1528.8594 1528.8613 -1.23 1 6 0.9 +1Score > 37 indicates identity
Score > 18 indicates homology
DPQVFSKVIDIIR
3206 696.3707 2086.0903 2086.0775 6.11 1 6 0.68 +1Score > 38 indicates identity
Score > 16 indicates homology
GLAWWNETVPVVLPYLTE
4191 937.1562 2808.4468 2808.4268 7.10 1 6 0.7 +1Score > 36 indicates identity
Score > 17 indicates homology
LWITRINAAAHSQGMNYSTFITALK + 2 Deamidated (NQ)
4009 658.8582 2631.4037 2631.4101 -2.43 1 6 1 +1Score > 35 indicates identity
Score > 18 indicates homology
FFGLFYAVGTPSLNPLIYTLRNK + Deamidated (NQ)
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Not what you expected? Try the select summary.