MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
306 331.6969 661.3792 661.3833 -6.06 0 29 0.47 +1Score > 38 indicates identity ISLMAK
1410 581.8039 1161.5932 1161.5990 -4.94 0 29 0.25 +1Score > 42 indicates identity
Score > 36 indicates homology
QTTSTGTPLTR
506 380.7202 759.4258 759.4126 17.4 0 29 0.62 +1Score > 42 indicates identity
Score > 40 indicates homology
LNDIASK
135 501.3169 500.3096 500.3071 5.08 0 29 0.24 +1Score > 43 indicates identity
Score > 36 indicates homology
AVVGR
568 394.2208 786.4270 786.4235 4.48 0 29 0.095 +1Score > 44 indicates identity
Score > 31 indicates homology
ANAQIIR + 2 Deamidated (NQ)
834 452.2444 902.4742 902.4821 -8.75 0 29 0.13 +1Score > 43 indicates identity
Score > 33 indicates homology
VTAGLDTAR
2125 751.4014 1500.7882 1500.8147 -17.6 1 29 0.24 +1Score > 40 indicates identity
Score > 35 indicates homology
QELQALTAALSASAK
962 318.5078 952.5016 952.4978 3.97 0 29 0.59 +1Score > 39 indicates identity QHVVLNDK + Deamidated (NQ)
1230 360.2146 1077.6220 1077.6182 3.46 1 29 0.053 +1Score > 36 indicates identity
Score > 29 indicates homology
VLLGPHVSEK
820 449.7428 897.4710 897.4668 4.70 0 29 0.13 +1Score > 39 indicates identity
Score > 33 indicates homology
QQLTGHSK
1145 516.2617 1030.5088 1030.4931 15.3 0 29 0.24 +1Score > 42 indicates identity
Score > 35 indicates homology
LTQNLAGGQE + Deamidated (NQ)
613 401.7263 801.4380 801.4344 4.49 1 29 0.25 +1Score > 43 indicates identity
Score > 35 indicates homology
GTNEILR
521 383.6912 765.3678 765.3657 2.80 1 29 0.12 +1Score > 42 indicates identity
Score > 32 indicates homology
VESFQR + Deamidated (NQ)
247 629.3654 628.3581 628.3544 5.90 0 29 0.13 +1Score > 37 indicates identity
Score > 32 indicates homology
AVNPTK
663 416.2487 830.4828 830.4862 -3.99 1 29 0.14 +1Score > 40 indicates identity
Score > 33 indicates homology
TLEVTLR
460 366.2073 730.4000 730.4086 -11.6 1 29 0.5 +1Score > 45 indicates identity
Score > 38 indicates homology
NNSIKR
1922 467.9246 1400.7520 1400.7623 -7.39 1 28 0.18 +1Score > 40 indicates identity
Score > 33 indicates homology
SVTQVNRDLLAGK + Deamidated (NQ)
418 713.4971 712.4898 712.4847 7.20 0 28 0.2 +1Score > 34 indicates identity AGVLLLK
427 716.3969 715.3896 715.3977 -11.3 0 28 0.26 +1Score > 43 indicates identity
Score > 35 indicates homology
QVSVAGR
1285 371.5471 1111.6195 1111.6138 5.09 0 28 0.55 +1Score > 38 indicates identity LADAHLFIGR
1565 615.3511 1228.6876 1228.6888 -0.91 1 28 0.5 +1Score > 40 indicates identity
Score > 38 indicates homology
LSDGRVLLNSR
1825 451.9460 1352.8162 1352.8279 -8.64 1 28 0.12 +1Score > 32 indicates identity KQTIAILPDLLK + Deamidated (NQ)
420 357.7301 713.4456 713.4436 2.91 0 28 0.57 +1Score > 40 indicates identity
Score > 38 indicates homology
VIDVLR
1067 499.3083 996.6020 996.5855 16.6 1 28 0.28 +1Score > 35 indicates identity EVIAIDLPK
1154 518.2802 1034.5458 1034.5284 16.9 1 28 0.79 +1Score > 41 indicates identity
Score > 40 indicates homology
AIIQGKQFE + 2 Deamidated (NQ)
3197 694.7191 2081.1355 2081.1157 9.52 1 28 0.32 +1Score > 36 indicates identity ILQEPHASLTEQYQALLK
718 429.2466 856.4786 856.4767 2.31 0 28 0.3 +1Score > 40 indicates identity
Score > 36 indicates homology
VALVDGQR
794 444.7455 887.4764 887.4712 5.89 1 28 0.69 +1Score > 43 indicates identity
Score > 39 indicates homology
LDQAKGGAK + Deamidated (NQ)
332 673.4017 672.3944 672.3918 3.83 1 28 0.36 +1Score > 41 indicates identity
Score > 36 indicates homology
KQGLAR + Deamidated (NQ)
915 468.2759 934.5372 934.5276 10.3 0 28 0.44 +1Score > 37 indicates identity NFLIPFGK
162 558.3640 557.3567 557.3537 5.46 0 28 0.6 +1Score > 40 indicates identity
Score > 38 indicates homology
AGIGLK
2128 751.4039 1500.7932 1500.8147 -14.3 1 28 0.19 +1Score > 40 indicates identity
Score > 33 indicates homology
QELQALTAALSASAK
841 302.8481 905.5225 905.5083 15.7 1 28 0.54 +1Score > 38 indicates identity IRFTVNR + Deamidated (NQ)
763 437.7511 873.4876 873.4920 -4.94 0 28 0.76 +1Score > 42 indicates identity
Score > 39 indicates homology
AQITTLAR + Deamidated (NQ)
792 444.2586 886.5026 886.5171 -16.3 1 28 0.81 +1Score > 42 indicates identity
Score > 39 indicates homology
QMARLLR
3895 837.0807 2508.2203 2508.1737 18.6 1 28 0.39 +1Score > 38 indicates identity
Score > 36 indicates homology
RMGQSGCDIVSVDWTVDLLDAR + Oxidation (M)
870 460.2552 918.4958 918.4844 12.4 1 28 0.85 +1Score > 41 indicates identity
Score > 40 indicates homology
IQMELIR + Deamidated (NQ); Oxidation (M)
1371 573.3047 1144.5948 1144.5910 3.36 0 28 0.11 +1Score > 42 indicates identity
Score > 31 indicates homology
IAMQIAAASPR + Deamidated (NQ); Oxidation (M)
993 322.1759 963.5059 963.5059 -0.019 1 28 0.11 +1Score > 42 indicates identity
Score > 31 indicates homology
TMKQAQLK + Deamidated (NQ); Oxidation (M)
611 401.2523 800.4900 800.4868 4.04 1 28 0.6 +1Score > 40 indicates identity
Score > 38 indicates homology
QSGLLRK
696 425.7162 849.4178 849.4344 -19.5 0 28 0.52 +1Score > 43 indicates identity
Score > 37 indicates homology
SLDAFAAR
258 631.3688 630.3615 630.3588 4.31 1 28 0.79 +1Score > 44 indicates identity
Score > 39 indicates homology
QIEIK + Deamidated (NQ)
771 440.2515 878.4884 878.4974 -10.2 1 28 0.17 +1Score > 39 indicates identity
Score > 33 indicates homology
AKFSTGIR
1528 608.3233 1214.6320 1214.6506 -15.3 1 28 0.13 +1Score > 41 indicates identity
Score > 31 indicates homology
AQNTALLENLK + Deamidated (NQ)
1023 976.5609 975.5536 975.5501 3.59 0 28 0.75 +1Score > 39 indicates identity
Score > 39 indicates homology
LANYIINR
2194 766.9424 1531.8702 1531.8473 15.0 0 28 0.074 +1Score > 35 indicates identity
Score > 29 indicates homology
AFLLGMYPVPTVPK
1940 470.5625 1408.6657 1408.6616 2.88 1 28 0.055 +1Score > 41 indicates identity
Score > 28 indicates homology
SASGDNMSKIIDR + Oxidation (M)
493 377.2177 752.4208 752.4181 3.68 0 28 0.11 +1Score > 38 indicates identity
Score > 30 indicates homology
ALDVHAK
444 364.2218 726.4290 726.4276 2.04 1 27 0.42 +1Score > 36 indicates identity IPQEIK
1021 488.7533 975.4920 975.5059 -14.2 1 27 0.33 +1Score > 42 indicates identity
Score > 35 indicates homology
KASVAMLGAE
4471 1046.8812 3137.6218 3137.5597 19.8 1 27 0.32 +1Score > 35 indicates identity YIDSIGVAEFVALADQYADLGPLYHPTAK + Deamidated (NQ)
1981 476.5970 1426.7692 1426.7892 -14.0 1 27 0.096 +1Score > 39 indicates identity
Score > 30 indicates homology
ARGIDIVLADTAGR
323 671.4493 670.4420 670.4377 6.38 0 27 0.46 +1Score > 37 indicates identity AGAVIIK
802 894.4330 893.4257 893.4243 1.63 1 27 0.96 +1Score > 42 indicates identity
Score > 40 indicates homology
ENSIFQR + Deamidated (NQ)
1430 586.8325 1171.6504 1171.6448 4.80 1 27 0.067 +1Score > 40 indicates identity
Score > 28 indicates homology
QLGLINEAVSK + Deamidated (NQ)
161 558.3634 557.3561 557.3537 4.38 0 27 0.59 +1Score > 40 indicates identity
Score > 37 indicates homology
AGAVLK
325 672.4073 671.4000 671.3966 5.07 0 27 0.78 +1Score > 42 indicates identity
Score > 39 indicates homology
LAQGVGK
133 499.3004 498.2931 498.2914 3.40 0 27 0.11 +1Score > 38 indicates identity
Score > 30 indicates homology
VAGPR
504 380.2539 758.4932 758.4902 4.05 0 27 0.089 +1Score > 30 indicates identity
Score > 29 indicates homology
VISILSK
762 437.7237 873.4328 873.4304 2.78 1 27 0.26 +1Score > 43 indicates identity
Score > 34 indicates homology
QSENIGAR
454 365.2255 728.4364 728.4333 4.30 0 27 0.9 +1Score > 41 indicates identity
Score > 39 indicates homology
IAWIAR
1562 614.8387 1227.6628 1227.6459 13.8 1 27 0.58 +1Score > 40 indicates identity
Score > 37 indicates homology
QAQQLLELQR + 2 Deamidated (NQ)
360 344.7045 687.3944 687.3915 4.25 1 27 0.54 +1Score > 42 indicates identity
Score > 37 indicates homology
LGLTER
868 460.2500 918.4854 918.4957 -11.1 1 27 0.67 +1Score > 42 indicates identity
Score > 38 indicates homology
VAKLMDAR + Oxidation (M)
973 479.7537 957.4928 957.5032 -10.8 0 27 0.28 +1Score > 41 indicates identity
Score > 34 indicates homology
VIQWQGAR + Deamidated (NQ)
1535 609.3509 1216.6872 1216.7027 -12.7 1 27 0.36 +1Score > 38 indicates identity
Score > 35 indicates homology
LSTLLKQGAGTK + Deamidated (NQ)
1289 557.8110 1113.6074 1113.6030 4.02 1 27 0.27 +1Score > 41 indicates identity
Score > 34 indicates homology
IAGTEAGISAPK
908 465.7695 929.5244 929.5182 6.77 1 27 0.52 +1Score > 42 indicates identity
Score > 36 indicates homology
KLANADIGK + Deamidated (NQ)
1042 494.7853 987.5560 987.5726 -16.8 1 27 0.83 +1Score > 40 indicates identity
Score > 38 indicates homology
LPHPAARAR
1899 695.8794 1389.7442 1389.7252 13.7 0 27 0.39 +1Score > 40 indicates identity
Score > 35 indicates homology
QFLQQLSQLQR + 2 Deamidated (NQ)
1261 548.2608 1094.5070 1094.5179 -9.89 0 27 0.59 +1Score > 41 indicates identity
Score > 37 indicates homology
VMNLAGFGNR + Deamidated (NQ); Oxidation (M)
1300 560.7936 1119.5726 1119.5924 -17.6 1 26 0.13 +1Score > 42 indicates identity
Score > 30 indicates homology
QFLNEILSR + Deamidated (NQ)
1307 374.5379 1120.5919 1120.5764 13.8 0 26 0.41 +1Score > 41 indicates identity
Score > 35 indicates homology
IQADGVYLNK + Deamidated (NQ)
807 447.7395 893.4644 893.4607 4.24 1 26 0.16 +1Score > 41 indicates identity
Score > 31 indicates homology
LSGELYGR
361 344.7065 687.3984 687.3915 10.1 0 26 0.92 +1Score > 42 indicates identity
Score > 39 indicates homology
IGQLTR + Deamidated (NQ)
934 471.7927 941.5708 941.5546 17.3 1 26 0.53 +1Score > 38 indicates identity
Score > 36 indicates homology
ILVKNQVE
1419 584.7751 1167.5356 1167.5482 -10.7 1 26 0.25 +1Score > 40 indicates identity
Score > 33 indicates homology
VDAQVFEMTK + Deamidated (NQ)
226 616.3693 615.3620 615.3592 4.65 1 26 0.77 +1Score > 45 indicates identity
Score > 38 indicates homology
VEGIAK
1492 400.5576 1198.6510 1198.6499 0.91 0 26 0.65 +1Score > 40 indicates identity
Score > 37 indicates homology
LGQFFTFLAR
994 482.7627 963.5108 963.5025 8.63 1 26 0.16 +1Score > 41 indicates identity
Score > 31 indicates homology
ALFETVQR + Deamidated (NQ)
393 351.7063 701.3980 701.4072 -13.0 0 26 0.71 +1Score > 43 indicates identity
Score > 37 indicates homology
VGAGATVK
1554 613.3333 1224.6520 1224.6462 4.75 1 26 0.15 +1Score > 39 indicates identity
Score > 31 indicates homology
QVAELINADPR
259 316.7036 631.3926 631.3905 3.45 0 26 0.19 +1Score > 31 indicates identity ITGTIK
662 416.2468 830.4790 830.4749 4.98 1 26 0.27 +1Score > 40 indicates identity
Score > 33 indicates homology
TLEQIVK + Deamidated (NQ)
1558 614.8382 1227.6618 1227.6459 13.0 1 26 0.85 +1Score > 40 indicates identity
Score > 38 indicates homology
QAQQLLELQR + 2 Deamidated (NQ)
417 357.2513 712.4880 712.4847 4.73 0 26 0.27 +1Score > 34 indicates identity
Score > 33 indicates homology
NIIILK
856 457.7578 913.5010 913.5093 -9.07 1 26 0.2 +1Score > 40 indicates identity
Score > 32 indicates homology
AGISRAPSR
1915 699.8879 1397.7612 1397.7514 7.04 1 26 0.39 +1Score > 39 indicates identity
Score > 34 indicates homology
VLALLNDNLQRE + Deamidated (NQ)
1040 494.2801 986.5456 986.5397 6.06 0 26 0.51 +1Score > 42 indicates identity
Score > 36 indicates homology
VGDTVVIQR + Deamidated (NQ)
1081 502.2818 1002.5490 1002.5346 14.5 0 26 0.47 +1Score > 41 indicates identity
Score > 35 indicates homology
VTNVAGINSK + Deamidated (NQ)
163 559.3232 558.3159 558.3238 -14.1 1 26 0.4 +1Score > 45 indicates identity
Score > 34 indicates homology
AGAKGR
326 336.7128 671.4110 671.4078 4.78 1 26 0.78 +1Score > 42 indicates identity
Score > 37 indicates homology
RVQIR + Deamidated (NQ)
837 452.7693 903.5240 903.5178 6.94 1 26 0.075 +1Score > 38 indicates identity
Score > 27 indicates homology
IEFLNIR
2124 751.4011 1500.7876 1500.8147 -18.0 1 26 0.27 +1Score > 40 indicates identity
Score > 33 indicates homology
QELQALTAALSASAK
1916 699.8887 1397.7628 1397.7514 8.19 1 26 0.24 +1Score > 39 indicates identity
Score > 32 indicates homology
VLALLNDNLQRE + Deamidated (NQ)
734 430.7477 859.4808 859.4651 18.4 0 26 0.6 +1Score > 42 indicates identity
Score > 36 indicates homology
IVDSNIAK + Deamidated (NQ)
845 454.7476 907.4806 907.4837 -3.38 0 26 0.9 +1Score > 41 indicates identity
Score > 38 indicates homology
SSMFVPIK
1140 515.3076 1028.6006 1028.6018 -1.16 1 26 0.33 +1Score > 39 indicates identity
Score > 33 indicates homology
LLLAPAFER
1959 710.3885 1418.7624 1418.7769 -10.2 0 26 0.7 +1Score > 40 indicates identity
Score > 36 indicates homology
LLTVLYPATGDTR
737 431.2396 860.4646 860.4603 5.02 0 25 0.3 +1Score > 42 indicates identity
Score > 33 indicates homology
TATIVNNK + Deamidated (NQ)
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