| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 331.6969 | 661.3792 | 661.3833 | -6.06 | 0 | 29 | 0.47 | 1Score > 38 indicates identity |
ISLMAK | |
| 581.8039 | 1161.5932 | 1161.5990 | -4.94 | 0 | 29 | 0.25 | 1Score > 42 indicates identityScore > 36 indicates homology |
QTTSTGTPLTR | |
| 380.7202 | 759.4258 | 759.4126 | 17.4 | 0 | 29 | 0.62 | 1Score > 42 indicates identityScore > 40 indicates homology |
LNDIASK | |
| 501.3169 | 500.3096 | 500.3071 | 5.08 | 0 | 29 | 0.24 | 1Score > 43 indicates identityScore > 36 indicates homology |
AVVGR | |
| 394.2208 | 786.4270 | 786.4235 | 4.48 | 0 | 29 | 0.095 | 1Score > 44 indicates identityScore > 31 indicates homology |
ANAQIIR + 2 Deamidated (NQ) | |
| 452.2444 | 902.4742 | 902.4821 | -8.75 | 0 | 29 | 0.13 | 1Score > 43 indicates identityScore > 33 indicates homology |
VTAGLDTAR | |
| 751.4014 | 1500.7882 | 1500.8147 | -17.6 | 1 | 29 | 0.24 | 1Score > 40 indicates identityScore > 35 indicates homology |
QELQALTAALSASAK | |
| 318.5078 | 952.5016 | 952.4978 | 3.97 | 0 | 29 | 0.59 | 1Score > 39 indicates identity |
QHVVLNDK + Deamidated (NQ) | |
| 360.2146 | 1077.6220 | 1077.6182 | 3.46 | 1 | 29 | 0.053 | 1Score > 36 indicates identityScore > 29 indicates homology |
VLLGPHVSEK | |
| 449.7428 | 897.4710 | 897.4668 | 4.70 | 0 | 29 | 0.13 | 1Score > 39 indicates identityScore > 33 indicates homology |
QQLTGHSK | |
| 516.2617 | 1030.5088 | 1030.4931 | 15.3 | 0 | 29 | 0.24 | 1Score > 42 indicates identityScore > 35 indicates homology |
LTQNLAGGQE + Deamidated (NQ) | |
| 401.7263 | 801.4380 | 801.4344 | 4.49 | 1 | 29 | 0.25 | 1Score > 43 indicates identityScore > 35 indicates homology |
GTNEILR | |
| 383.6912 | 765.3678 | 765.3657 | 2.80 | 1 | 29 | 0.12 | 1Score > 42 indicates identityScore > 32 indicates homology |
VESFQR + Deamidated (NQ) | |
| 629.3654 | 628.3581 | 628.3544 | 5.90 | 0 | 29 | 0.13 | 1Score > 37 indicates identityScore > 32 indicates homology |
AVNPTK | |
| 416.2487 | 830.4828 | 830.4862 | -3.99 | 1 | 29 | 0.14 | 1Score > 40 indicates identityScore > 33 indicates homology |
TLEVTLR | |
| 366.2073 | 730.4000 | 730.4086 | -11.6 | 1 | 29 | 0.5 | 1Score > 45 indicates identityScore > 38 indicates homology |
NNSIKR | |
| 467.9246 | 1400.7520 | 1400.7623 | -7.39 | 1 | 28 | 0.18 | 1Score > 40 indicates identityScore > 33 indicates homology |
SVTQVNRDLLAGK + Deamidated (NQ) | |
| 713.4971 | 712.4898 | 712.4847 | 7.20 | 0 | 28 | 0.2 | 1Score > 34 indicates identity |
AGVLLLK | |
| 716.3969 | 715.3896 | 715.3977 | -11.3 | 0 | 28 | 0.26 | 1Score > 43 indicates identityScore > 35 indicates homology |
QVSVAGR | |
| 371.5471 | 1111.6195 | 1111.6138 | 5.09 | 0 | 28 | 0.55 | 1Score > 38 indicates identity |
LADAHLFIGR | |
| 615.3511 | 1228.6876 | 1228.6888 | -0.91 | 1 | 28 | 0.5 | 1Score > 40 indicates identityScore > 38 indicates homology |
LSDGRVLLNSR | |
| 451.9460 | 1352.8162 | 1352.8279 | -8.64 | 1 | 28 | 0.12 | 1Score > 32 indicates identity |
KQTIAILPDLLK + Deamidated (NQ) | |
| 357.7301 | 713.4456 | 713.4436 | 2.91 | 0 | 28 | 0.57 | 1Score > 40 indicates identityScore > 38 indicates homology |
VIDVLR | |
| 499.3083 | 996.6020 | 996.5855 | 16.6 | 1 | 28 | 0.28 | 1Score > 35 indicates identity |
EVIAIDLPK | |
| 518.2802 | 1034.5458 | 1034.5284 | 16.9 | 1 | 28 | 0.79 | 1Score > 41 indicates identityScore > 40 indicates homology |
AIIQGKQFE + 2 Deamidated (NQ) | |
| 694.7191 | 2081.1355 | 2081.1157 | 9.52 | 1 | 28 | 0.32 | 1Score > 36 indicates identity |
ILQEPHASLTEQYQALLK | |
| 429.2466 | 856.4786 | 856.4767 | 2.31 | 0 | 28 | 0.3 | 1Score > 40 indicates identityScore > 36 indicates homology |
VALVDGQR | |
| 444.7455 | 887.4764 | 887.4712 | 5.89 | 1 | 28 | 0.69 | 1Score > 43 indicates identityScore > 39 indicates homology |
LDQAKGGAK + Deamidated (NQ) | |
| 673.4017 | 672.3944 | 672.3918 | 3.83 | 1 | 28 | 0.36 | 1Score > 41 indicates identityScore > 36 indicates homology |
KQGLAR + Deamidated (NQ) | |
| 468.2759 | 934.5372 | 934.5276 | 10.3 | 0 | 28 | 0.44 | 1Score > 37 indicates identity |
NFLIPFGK | |
| 558.3640 | 557.3567 | 557.3537 | 5.46 | 0 | 28 | 0.6 | 1Score > 40 indicates identityScore > 38 indicates homology |
AGIGLK | |
| 751.4039 | 1500.7932 | 1500.8147 | -14.3 | 1 | 28 | 0.19 | 1Score > 40 indicates identityScore > 33 indicates homology |
QELQALTAALSASAK | |
| 302.8481 | 905.5225 | 905.5083 | 15.7 | 1 | 28 | 0.54 | 1Score > 38 indicates identity |
IRFTVNR + Deamidated (NQ) | |
| 437.7511 | 873.4876 | 873.4920 | -4.94 | 0 | 28 | 0.76 | 1Score > 42 indicates identityScore > 39 indicates homology |
AQITTLAR + Deamidated (NQ) | |
| 444.2586 | 886.5026 | 886.5171 | -16.3 | 1 | 28 | 0.81 | 1Score > 42 indicates identityScore > 39 indicates homology |
QMARLLR | |
| 837.0807 | 2508.2203 | 2508.1737 | 18.6 | 1 | 28 | 0.39 | 1Score > 38 indicates identityScore > 36 indicates homology |
RMGQSGCDIVSVDWTVDLLDAR + Oxidation (M) | |
| 460.2552 | 918.4958 | 918.4844 | 12.4 | 1 | 28 | 0.85 | 1Score > 41 indicates identityScore > 40 indicates homology |
IQMELIR + Deamidated (NQ); Oxidation (M) | |
| 573.3047 | 1144.5948 | 1144.5910 | 3.36 | 0 | 28 | 0.11 | 1Score > 42 indicates identityScore > 31 indicates homology |
IAMQIAAASPR + Deamidated (NQ); Oxidation (M) | |
| 322.1759 | 963.5059 | 963.5059 | -0.019 | 1 | 28 | 0.11 | 1Score > 42 indicates identityScore > 31 indicates homology |
TMKQAQLK + Deamidated (NQ); Oxidation (M) | |
| 401.2523 | 800.4900 | 800.4868 | 4.04 | 1 | 28 | 0.6 | 1Score > 40 indicates identityScore > 38 indicates homology |
QSGLLRK | |
| 425.7162 | 849.4178 | 849.4344 | -19.5 | 0 | 28 | 0.52 | 1Score > 43 indicates identityScore > 37 indicates homology |
SLDAFAAR | |
| 631.3688 | 630.3615 | 630.3588 | 4.31 | 1 | 28 | 0.79 | 1Score > 44 indicates identityScore > 39 indicates homology |
QIEIK + Deamidated (NQ) | |
| 440.2515 | 878.4884 | 878.4974 | -10.2 | 1 | 28 | 0.17 | 1Score > 39 indicates identityScore > 33 indicates homology |
AKFSTGIR | |
| 608.3233 | 1214.6320 | 1214.6506 | -15.3 | 1 | 28 | 0.13 | 1Score > 41 indicates identityScore > 31 indicates homology |
AQNTALLENLK + Deamidated (NQ) | |
| 976.5609 | 975.5536 | 975.5501 | 3.59 | 0 | 28 | 0.75 | 1Score > 39 indicates identityScore > 39 indicates homology |
LANYIINR | |
| 766.9424 | 1531.8702 | 1531.8473 | 15.0 | 0 | 28 | 0.074 | 1Score > 35 indicates identityScore > 29 indicates homology |
AFLLGMYPVPTVPK | |
| 470.5625 | 1408.6657 | 1408.6616 | 2.88 | 1 | 28 | 0.055 | 1Score > 41 indicates identityScore > 28 indicates homology |
SASGDNMSKIIDR + Oxidation (M) | |
| 377.2177 | 752.4208 | 752.4181 | 3.68 | 0 | 28 | 0.11 | 1Score > 38 indicates identityScore > 30 indicates homology |
ALDVHAK | |
| 364.2218 | 726.4290 | 726.4276 | 2.04 | 1 | 27 | 0.42 | 1Score > 36 indicates identity |
IPQEIK | |
| 488.7533 | 975.4920 | 975.5059 | -14.2 | 1 | 27 | 0.33 | 1Score > 42 indicates identityScore > 35 indicates homology |
KASVAMLGAE | |
| 1046.8812 | 3137.6218 | 3137.5597 | 19.8 | 1 | 27 | 0.32 | 1Score > 35 indicates identity |
YIDSIGVAEFVALADQYADLGPLYHPTAK + Deamidated (NQ) | |
| 476.5970 | 1426.7692 | 1426.7892 | -14.0 | 1 | 27 | 0.096 | 1Score > 39 indicates identityScore > 30 indicates homology |
ARGIDIVLADTAGR | |
| 671.4493 | 670.4420 | 670.4377 | 6.38 | 0 | 27 | 0.46 | 1Score > 37 indicates identity |
AGAVIIK | |
| 894.4330 | 893.4257 | 893.4243 | 1.63 | 1 | 27 | 0.96 | 1Score > 42 indicates identityScore > 40 indicates homology |
ENSIFQR + Deamidated (NQ) | |
| 586.8325 | 1171.6504 | 1171.6448 | 4.80 | 1 | 27 | 0.067 | 1Score > 40 indicates identityScore > 28 indicates homology |
QLGLINEAVSK + Deamidated (NQ) | |
| 558.3634 | 557.3561 | 557.3537 | 4.38 | 0 | 27 | 0.59 | 1Score > 40 indicates identityScore > 37 indicates homology |
AGAVLK | |
| 672.4073 | 671.4000 | 671.3966 | 5.07 | 0 | 27 | 0.78 | 1Score > 42 indicates identityScore > 39 indicates homology |
LAQGVGK | |
| 499.3004 | 498.2931 | 498.2914 | 3.40 | 0 | 27 | 0.11 | 1Score > 38 indicates identityScore > 30 indicates homology |
VAGPR | |
| 380.2539 | 758.4932 | 758.4902 | 4.05 | 0 | 27 | 0.089 | 1Score > 30 indicates identityScore > 29 indicates homology |
VISILSK | |
| 437.7237 | 873.4328 | 873.4304 | 2.78 | 1 | 27 | 0.26 | 1Score > 43 indicates identityScore > 34 indicates homology |
QSENIGAR | |
| 365.2255 | 728.4364 | 728.4333 | 4.30 | 0 | 27 | 0.9 | 1Score > 41 indicates identityScore > 39 indicates homology |
IAWIAR | |
| 614.8387 | 1227.6628 | 1227.6459 | 13.8 | 1 | 27 | 0.58 | 1Score > 40 indicates identityScore > 37 indicates homology |
QAQQLLELQR + 2 Deamidated (NQ) | |
| 344.7045 | 687.3944 | 687.3915 | 4.25 | 1 | 27 | 0.54 | 1Score > 42 indicates identityScore > 37 indicates homology |
LGLTER | |
| 460.2500 | 918.4854 | 918.4957 | -11.1 | 1 | 27 | 0.67 | 1Score > 42 indicates identityScore > 38 indicates homology |
VAKLMDAR + Oxidation (M) | |
| 479.7537 | 957.4928 | 957.5032 | -10.8 | 0 | 27 | 0.28 | 1Score > 41 indicates identityScore > 34 indicates homology |
VIQWQGAR + Deamidated (NQ) | |
| 609.3509 | 1216.6872 | 1216.7027 | -12.7 | 1 | 27 | 0.36 | 1Score > 38 indicates identityScore > 35 indicates homology |
LSTLLKQGAGTK + Deamidated (NQ) | |
| 557.8110 | 1113.6074 | 1113.6030 | 4.02 | 1 | 27 | 0.27 | 1Score > 41 indicates identityScore > 34 indicates homology |
IAGTEAGISAPK | |
| 465.7695 | 929.5244 | 929.5182 | 6.77 | 1 | 27 | 0.52 | 1Score > 42 indicates identityScore > 36 indicates homology |
KLANADIGK + Deamidated (NQ) | |
| 494.7853 | 987.5560 | 987.5726 | -16.8 | 1 | 27 | 0.83 | 1Score > 40 indicates identityScore > 38 indicates homology |
LPHPAARAR | |
| 695.8794 | 1389.7442 | 1389.7252 | 13.7 | 0 | 27 | 0.39 | 1Score > 40 indicates identityScore > 35 indicates homology |
QFLQQLSQLQR + 2 Deamidated (NQ) | |
| 548.2608 | 1094.5070 | 1094.5179 | -9.89 | 0 | 27 | 0.59 | 1Score > 41 indicates identityScore > 37 indicates homology |
VMNLAGFGNR + Deamidated (NQ); Oxidation (M) | |
| 560.7936 | 1119.5726 | 1119.5924 | -17.6 | 1 | 26 | 0.13 | 1Score > 42 indicates identityScore > 30 indicates homology |
QFLNEILSR + Deamidated (NQ) | |
| 374.5379 | 1120.5919 | 1120.5764 | 13.8 | 0 | 26 | 0.41 | 1Score > 41 indicates identityScore > 35 indicates homology |
IQADGVYLNK + Deamidated (NQ) | |
| 447.7395 | 893.4644 | 893.4607 | 4.24 | 1 | 26 | 0.16 | 1Score > 41 indicates identityScore > 31 indicates homology |
LSGELYGR | |
| 344.7065 | 687.3984 | 687.3915 | 10.1 | 0 | 26 | 0.92 | 1Score > 42 indicates identityScore > 39 indicates homology |
IGQLTR + Deamidated (NQ) | |
| 471.7927 | 941.5708 | 941.5546 | 17.3 | 1 | 26 | 0.53 | 1Score > 38 indicates identityScore > 36 indicates homology |
ILVKNQVE | |
| 584.7751 | 1167.5356 | 1167.5482 | -10.7 | 1 | 26 | 0.25 | 1Score > 40 indicates identityScore > 33 indicates homology |
VDAQVFEMTK + Deamidated (NQ) | |
| 616.3693 | 615.3620 | 615.3592 | 4.65 | 1 | 26 | 0.77 | 1Score > 45 indicates identityScore > 38 indicates homology |
VEGIAK | |
| 400.5576 | 1198.6510 | 1198.6499 | 0.91 | 0 | 26 | 0.65 | 1Score > 40 indicates identityScore > 37 indicates homology |
LGQFFTFLAR | |
| 482.7627 | 963.5108 | 963.5025 | 8.63 | 1 | 26 | 0.16 | 1Score > 41 indicates identityScore > 31 indicates homology |
ALFETVQR + Deamidated (NQ) | |
| 351.7063 | 701.3980 | 701.4072 | -13.0 | 0 | 26 | 0.71 | 1Score > 43 indicates identityScore > 37 indicates homology |
VGAGATVK | |
| 613.3333 | 1224.6520 | 1224.6462 | 4.75 | 1 | 26 | 0.15 | 1Score > 39 indicates identityScore > 31 indicates homology |
QVAELINADPR | |
| 316.7036 | 631.3926 | 631.3905 | 3.45 | 0 | 26 | 0.19 | 1Score > 31 indicates identity |
ITGTIK | |
| 416.2468 | 830.4790 | 830.4749 | 4.98 | 1 | 26 | 0.27 | 1Score > 40 indicates identityScore > 33 indicates homology |
TLEQIVK + Deamidated (NQ) | |
| 614.8382 | 1227.6618 | 1227.6459 | 13.0 | 1 | 26 | 0.85 | 1Score > 40 indicates identityScore > 38 indicates homology |
QAQQLLELQR + 2 Deamidated (NQ) | |
| 357.2513 | 712.4880 | 712.4847 | 4.73 | 0 | 26 | 0.27 | 1Score > 34 indicates identityScore > 33 indicates homology |
NIIILK | |
| 457.7578 | 913.5010 | 913.5093 | -9.07 | 1 | 26 | 0.2 | 1Score > 40 indicates identityScore > 32 indicates homology |
AGISRAPSR | |
| 699.8879 | 1397.7612 | 1397.7514 | 7.04 | 1 | 26 | 0.39 | 1Score > 39 indicates identityScore > 34 indicates homology |
VLALLNDNLQRE + Deamidated (NQ) | |
| 494.2801 | 986.5456 | 986.5397 | 6.06 | 0 | 26 | 0.51 | 1Score > 42 indicates identityScore > 36 indicates homology |
VGDTVVIQR + Deamidated (NQ) | |
| 502.2818 | 1002.5490 | 1002.5346 | 14.5 | 0 | 26 | 0.47 | 1Score > 41 indicates identityScore > 35 indicates homology |
VTNVAGINSK + Deamidated (NQ) | |
| 559.3232 | 558.3159 | 558.3238 | -14.1 | 1 | 26 | 0.4 | 1Score > 45 indicates identityScore > 34 indicates homology |
AGAKGR | |
| 336.7128 | 671.4110 | 671.4078 | 4.78 | 1 | 26 | 0.78 | 1Score > 42 indicates identityScore > 37 indicates homology |
RVQIR + Deamidated (NQ) | |
| 452.7693 | 903.5240 | 903.5178 | 6.94 | 1 | 26 | 0.075 | 1Score > 38 indicates identityScore > 27 indicates homology |
IEFLNIR | |
| 751.4011 | 1500.7876 | 1500.8147 | -18.0 | 1 | 26 | 0.27 | 1Score > 40 indicates identityScore > 33 indicates homology |
QELQALTAALSASAK | |
| 699.8887 | 1397.7628 | 1397.7514 | 8.19 | 1 | 26 | 0.24 | 1Score > 39 indicates identityScore > 32 indicates homology |
VLALLNDNLQRE + Deamidated (NQ) | |
| 430.7477 | 859.4808 | 859.4651 | 18.4 | 0 | 26 | 0.6 | 1Score > 42 indicates identityScore > 36 indicates homology |
IVDSNIAK + Deamidated (NQ) | |
| 454.7476 | 907.4806 | 907.4837 | -3.38 | 0 | 26 | 0.9 | 1Score > 41 indicates identityScore > 38 indicates homology |
SSMFVPIK | |
| 515.3076 | 1028.6006 | 1028.6018 | -1.16 | 1 | 26 | 0.33 | 1Score > 39 indicates identityScore > 33 indicates homology |
LLLAPAFER | |
| 710.3885 | 1418.7624 | 1418.7769 | -10.2 | 0 | 26 | 0.7 | 1Score > 40 indicates identityScore > 36 indicates homology |
LLTVLYPATGDTR | |
| 431.2396 | 860.4646 | 860.4603 | 5.02 | 0 | 25 | 0.3 | 1Score > 42 indicates identityScore > 33 indicates homology |
TATIVNNK + Deamidated (NQ) |
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