MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
408 706.3663 705.3590 705.3479 15.7 1 7 0.71 +1Score > 42 indicates identity
Score > 18 indicates homology
EGLMTR
1177 1046.5333 1045.5260 1045.5444 -17.6 0 7 0.79 +1Score > 42 indicates identity
Score > 19 indicates homology
ALASLSGWLE
4316 737.8773 2947.4801 2947.4420 12.9 1 7 1 +1Score > 37 indicates identity
Score > 20 indicates homology
RADGYHQVVMVMQTIALADTVTVAVNE + Deamidated (NQ); Oxidation (M)
2278 524.2902 1569.8488 1569.8549 -3.88 1 7 0.79 +1Score > 39 indicates identity
Score > 19 indicates homology
QLKDGGIMVIPIGGR + Deamidated (NQ); Oxidation (M)
4304 586.3073 2926.5001 2926.4560 15.1 1 7 0.44 +1Score > 36 indicates identity
Score > 16 indicates homology
EPETYGHLTLNDIVQSLSADAQALNVK + Deamidated (NQ)
351 342.2265 682.4384 682.4377 1.05 0 7 0.97 +1Score > 34 indicates identity
Score > 19 indicates homology
AIAALPK
859 458.2768 914.5390 914.5338 5.74 0 7 0.93 +1Score > 40 indicates identity
Score > 19 indicates homology
GPAGVFIVR
2318 529.2911 1584.8515 1584.8247 16.9 0 7 0.92 +1Score > 39 indicates identity
Score > 19 indicates homology
AIQQVTGAQGVAVVIE + 3 Deamidated (NQ)
2057 735.3710 1468.7274 1468.7231 2.95 1 7 0.91 +1Score > 40 indicates identity
Score > 19 indicates homology
ANQLELPPMLANR + 3 Deamidated (NQ)
2573 852.4740 1702.9334 1702.9552 -12.8 0 7 0.85 +1Score > 37 indicates identity
Score > 19 indicates homology
VLVMQVNPDVLLHAR
3379 543.5511 2170.1753 2170.1707 2.10 1 7 0.33 +1Score > 36 indicates identity
Score > 15 indicates homology
LFLAPLPCTELSLGQQLLR + 2 Deamidated (NQ)
3763 796.0931 2385.2575 2385.2613 -1.61 1 7 0.32 +1Score > 37 indicates identity
Score > 15 indicates homology
LAQVAVNMDQTLAAWLAKKPSK + 3 Deamidated (NQ)
2650 581.6688 1741.9846 1741.9574 15.6 1 7 0.46 +1Score > 34 indicates identity
Score > 16 indicates homology
ALSVQVAVTAQRIQQK + 3 Deamidated (NQ)
2095 746.3875 1490.7604 1490.7552 3.55 0 7 0.82 +1Score > 41 indicates identity
Score > 19 indicates homology
HDLSFLSTVMLGR + Oxidation (M)
3030 658.3746 1972.1020 1972.1132 -5.69 1 7 0.27 +1Score > 34 indicates identity
Score > 14 indicates homology
IILVILDAISNIFQAAEK + 2 Deamidated (NQ)
3845 821.4476 2461.3210 2461.3475 -10.8 1 7 0.32 +1Score > 35 indicates identity
Score > 15 indicates homology
ATAALQRMGYLLVLVTNQSGIAR + Oxidation (M)
1079 1002.5482 1001.5409 1001.5328 8.09 0 7 0.73 +1Score > 42 indicates identity
Score > 18 indicates homology
TVICGPVTR
4285 727.6278 2906.4821 2906.4749 2.49 1 7 0.57 +1Score > 36 indicates identity
Score > 17 indicates homology
FPASPGPQQALMPPNLWSLRAKPGTAR + 3 Deamidated (NQ); Oxidation (M)
2676 882.4828 1762.9510 1762.9465 2.58 0 7 1 +1Score > 38 indicates identity
Score > 19 indicates homology
LLLNGTVQGSTYLDLR + Deamidated (NQ)
4310 734.3824 2933.5005 2933.5208 -6.94 0 7 0.4 +1Score > 36 indicates identity
Score > 15 indicates homology
VAAGYGHAPNTLTDPVVLALPGTLPVMNK + 2 Deamidated (NQ); Oxidation (M)
1854 684.3566 1366.6986 1366.7092 -7.74 1 7 0.88 +1Score > 40 indicates identity
Score > 19 indicates homology
ITDVYTNSLRGK + Deamidated (NQ)
3731 788.3907 2362.1503 2362.1726 -9.45 1 7 0.74 +1Score > 39 indicates identity
Score > 18 indicates homology
QSILLEFVSANPTGPMNIVSAR + 3 Deamidated (NQ); Oxidation (M)
2472 826.4380 1650.8614 1650.8689 -4.51 1 7 0.42 +1Score > 40 indicates identity
Score > 16 indicates homology
QSRLGLQPQQGPLAR + 3 Deamidated (NQ)
622 405.7284 809.4422 809.4395 3.34 1 7 0.44 +1Score > 38 indicates identity
Score > 16 indicates homology
TSTKAFR
2932 637.6682 1909.9828 1909.9547 14.7 1 7 0.8 +1Score > 39 indicates identity
Score > 18 indicates homology
FYNQHLINHVGRGTPR + 2 Deamidated (NQ)
3858 618.5410 2470.1349 2470.1122 9.20 1 7 0.26 +1Score > 38 indicates identity
Score > 14 indicates homology
ANLLNQTSPNIQISSQNYTTEK + 7 Deamidated (NQ)
1401 1159.7114 1158.7041 1158.7046 -0.40 1 7 0.42 +1Score > 31 indicates identity
Score > 16 indicates homology
SLPLLMITKK + Oxidation (M)
1911 698.8432 1395.6718 1395.6994 -19.7 0 7 0.73 +1Score > 41 indicates identity
Score > 18 indicates homology
VNLDHTNIDINK + Deamidated (NQ)
3150 1023.5351 2045.0556 2045.0840 -13.9 1 7 0.96 +1Score > 39 indicates identity
Score > 19 indicates homology
GVFSMQQRQALAQISLPR + Oxidation (M)
2644 578.9670 1733.8792 1733.8584 12.0 1 7 0.69 +1Score > 40 indicates identity
Score > 18 indicates homology
LNGSAVDFDVNDKALR + Deamidated (NQ)
3760 795.7171 2384.1295 2384.1092 8.51 0 7 0.3 +1Score > 38 indicates identity
Score > 14 indicates homology
ALSNQPIVTVSADDQDDTANGPR + Deamidated (NQ)
3866 826.4448 2476.3126 2476.2996 5.25 1 7 0.4 +1Score > 36 indicates identity
Score > 15 indicates homology
VPTLDGHVKLTIPAGTQPNTMLR + 2 Deamidated (NQ); Oxidation (M)
1455 591.3672 1180.7198 1180.7179 1.62 1 7 0.97 +1Score > 32 indicates identity
Score > 19 indicates homology
NKLTLGLPTPK
1458 591.3688 1180.7230 1180.7067 13.8 1 7 0.85 +1Score > 31 indicates identity
Score > 19 indicates homology
EIIQVLQLPK + Deamidated (NQ)
3215 1046.5189 2091.0232 2091.0194 1.84 1 7 0.44 +1Score > 40 indicates identity
Score > 16 indicates homology
MSTDTALVIGLGNPGPQYEK + Deamidated (NQ)
2775 906.9428 1811.8710 1811.8359 19.4 1 7 0.8 +1Score > 40 indicates identity
Score > 18 indicates homology
ADGLAMSSRNLYLSADE
2972 966.0442 1930.0738 1930.0445 15.2 1 7 0.81 +1Score > 34 indicates identity
Score > 18 indicates homology
VQVGLVGDAQVGKTSLMVK + 2 Deamidated (NQ)
3342 717.0225 2148.0457 2148.0198 12.0 0 7 0.49 +1Score > 39 indicates identity
Score > 16 indicates homology
FSFSALVVVGDGNGSVGFGMGK + Deamidated (NQ); Oxidation (M)
3837 818.4249 2452.2529 2452.2169 14.7 1 7 0.47 +1Score > 38 indicates identity
Score > 16 indicates homology
HAIAQGVNPVTAFQMATLNPAER + Deamidated (NQ); Oxidation (M)
2435 816.4545 1630.8944 1630.8719 13.8 0 7 0.78 +1Score > 37 indicates identity
Score > 18 indicates homology
LTLHGLQQYFVALE
4333 744.3856 2973.5133 2973.5059 2.49 1 7 0.61 +1Score > 36 indicates identity
Score > 17 indicates homology
HGLYDDIWQAFAVLLPVKTVGVMGDGR + Deamidated (NQ); Oxidation (M)
3693 780.0762 2337.2068 2337.1753 13.5 1 7 0.47 +1Score > 37 indicates identity
Score > 16 indicates homology
STNPLVWQQATVRIQQFYR + 3 Deamidated (NQ)
2574 569.3124 1704.9154 1704.9199 -2.65 1 7 0.65 +1Score > 38 indicates identity
Score > 17 indicates homology
TVGAPLYAAFQEGLLR
4724 921.9816 3683.8973 3683.8928 1.23 1 7 1 +1Score > 33 indicates identity
Score > 19 indicates homology
MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSR + Deamidated (NQ)
2170 759.9199 1517.8252 1517.8309 -3.75 0 7 0.64 +1Score > 38 indicates identity
Score > 17 indicates homology
LLKPTVMMNLLGR + Deamidated (NQ); 2 Oxidation (M)
3655 771.0652 2310.1738 2310.2080 -14.8 1 7 0.45 +1Score > 38 indicates identity
Score > 16 indicates homology
IDFSSIAVPGTSNPQQRQLPR
2806 611.3381 1830.9925 1830.9839 4.66 1 7 0.47 +1Score > 37 indicates identity
Score > 16 indicates homology
IAVVQNGIIENYQTLR + Deamidated (NQ)
4496 1061.5232 3181.5478 3181.5635 -4.94 1 7 0.47 +1Score > 36 indicates identity
Score > 16 indicates homology
EGAQQMGLQLMDSPTPIQPIVIGDSAQALR + 2 Deamidated (NQ); Oxidation (M)
2761 602.9741 1805.9005 1805.8908 5.38 1 7 0.64 +1Score > 41 indicates identity
Score > 17 indicates homology
QNLAFQSGQVDINKSR + 2 Deamidated (NQ)
3966 860.4087 2578.2043 2578.1764 10.8 1 7 0.61 +1Score > 38 indicates identity
Score > 17 indicates homology
RAYQAASWAWAQQVFPDLDPQE + 2 Deamidated (NQ)
2261 782.3651 1562.7156 1562.7439 -18.1 0 7 0.77 +1Score > 40 indicates identity
Score > 18 indicates homology
HLNINNMPVYAFK + 3 Deamidated (NQ)
3207 696.3723 2086.0951 2086.1350 -19.2 1 7 0.43 +1Score > 38 indicates identity
Score > 15 indicates homology
IAQLVFVPVVQAEFNIVAE + Deamidated (NQ)
3838 818.4257 2452.2553 2452.2169 15.7 1 7 0.58 +1Score > 38 indicates identity
Score > 17 indicates homology
HAIAQGVNPVTAFQMATLNPAER + Deamidated (NQ); Oxidation (M)
4640 858.4337 3429.7057 3429.6831 6.58 1 7 0.37 +1Score > 35 indicates identity
Score > 15 indicates homology
NVNQCILEMLIAAPDTMSVTLYFMLLLVAE + 2 Deamidated (NQ); Oxidation (M)
3772 599.5807 2394.2937 2394.2770 6.99 1 7 0.3 +1Score > 35 indicates identity
Score > 14 indicates homology
AIFPGIQGGPLMHVIAAKAVAFGE + Deamidated (NQ)
843 908.4608 907.4535 907.4651 -12.7 0 7 0.38 +1Score > 42 indicates identity
Score > 15 indicates homology
GGVLSYLAE
4010 658.8679 2631.4425 2631.4127 11.3 1 7 0.93 +1Score > 32 indicates identity
Score > 19 indicates homology
NLGSALSYMLANKLASPIMPAIAIR + Deamidated (NQ); Oxidation (M)
4446 1040.2064 3117.5974 3117.6459 -15.6 1 7 0.43 +1Score > 35 indicates identity
Score > 15 indicates homology
LGIPVVAILDSNSDPRGVTFPIPGNDDAIR
3143 681.0296 2040.0670 2040.0310 17.6 1 7 0.51 +1Score > 38 indicates identity
Score > 16 indicates homology
VNVDNRFATVTLSATTGMK + Oxidation (M)
1862 457.9156 1370.7250 1370.7405 -11.3 1 7 0.81 +1Score > 40 indicates identity
Score > 18 indicates homology
LNIDNNDKAILK + Deamidated (NQ)
2963 642.3181 1923.9325 1923.9361 -1.86 1 7 0.69 +1Score > 40 indicates identity
Score > 17 indicates homology
DGTVRYDMTDLPVTAVR + Oxidation (M)
2030 726.4091 1450.8036 1450.7854 12.6 0 7 0.96 +1Score > 37 indicates identity
Score > 19 indicates homology
VMLTALSQIVGFR + Deamidated (NQ); Oxidation (M)
2559 849.4294 1696.8442 1696.8268 10.3 1 7 0.64 +1Score > 40 indicates identity
Score > 17 indicates homology
NAVPVIQDIQQQGER + 3 Deamidated (NQ)
2445 409.7291 1634.8873 1634.8628 15.0 1 7 0.91 +1Score > 37 indicates identity
Score > 19 indicates homology
ISIDDFGTGLSNLKR
3387 1087.0461 2172.0776 2172.0806 -1.36 1 7 0.48 +1Score > 39 indicates identity
Score > 16 indicates homology
IPLLPENNMSASIDCAGILK + Deamidated (NQ); Oxidation (M)
3592 1138.5198 2275.0250 2275.0573 -14.2 1 7 0.79 +1Score > 37 indicates identity
Score > 18 indicates homology
NCSVTICHSKTHNLSSITSK + 2 Deamidated (NQ)
4469 628.5180 3137.5536 3137.5703 -5.31 1 7 0.74 +1Score > 36 indicates identity
Score > 18 indicates homology
SMRPLAIDTAHQLIDNFEQKPSVDLVAE + Deamidated (NQ)
744 434.2060 866.3974 866.4134 -18.4 1 7 0.82 +1Score > 41 indicates identity
Score > 18 indicates homology
VENSVYR + Deamidated (NQ)
2188 765.4350 1528.8554 1528.8501 3.52 1 7 0.8 +1Score > 37 indicates identity
Score > 18 indicates homology
KLPQVAKPLTFASE + Deamidated (NQ)
2566 567.6312 1699.8718 1699.8563 9.09 0 7 0.58 +1Score > 40 indicates identity
Score > 17 indicates homology
GVDIPQVLVSQSCAAR + Deamidated (NQ)
3144 681.6789 2042.0149 2042.0353 -10.0 1 7 0.99 +1Score > 39 indicates identity
Score > 19 indicates homology
NQLAALMKALPLGQQAAQR + 5 Deamidated (NQ); Oxidation (M)
4818 1013.0288 4048.0861 4048.1279 -10.3 1 7 1 +1Score > 32 indicates identity
Score > 19 indicates homology
TIGPQTHLVKAGTPTMGGVLVLVSIAVSTLLWCDWGNR + Deamidated (NQ)
3203 696.0665 2085.1777 2085.1431 16.6 0 7 0.59 +1Score > 32 indicates identity
Score > 17 indicates homology
LLPALAGALSVPMAYQIVLE + Deamidated (NQ); Oxidation (M)
2867 623.9695 1868.8867 1868.9057 -10.2 1 7 0.59 +1Score > 40 indicates identity
Score > 17 indicates homology
WNLLDDKNDFISFSR
3192 693.3752 2077.1038 2077.0666 17.9 1 7 0.75 +1Score > 37 indicates identity
Score > 18 indicates homology
NGITPLGGFLARMYTLQPE
2112 749.4056 1496.7966 1496.7763 13.6 1 7 0.73 +1Score > 39 indicates identity
Score > 18 indicates homology
VFLTPEQIDPLPE
2407 809.3969 1616.7792 1616.8046 -15.7 1 7 0.87 +1Score > 40 indicates identity
Score > 18 indicates homology
IYVKHTGQDLDTVE
4159 697.8671 2787.4393 2787.4225 6.03 0 7 0.47 +1Score > 36 indicates identity
Score > 16 indicates homology
ADIMATNGVVHTINTVLRPPANKPQE + 2 Deamidated (NQ)
2792 456.4884 1821.9245 1821.9083 8.88 1 6 0.89 +1Score > 40 indicates identity
Score > 18 indicates homology
GMWPPPNNLLNQSPKK + 2 Deamidated (NQ)
3579 1134.0798 2266.1450 2266.1805 -15.6 1 6 0.35 +1Score > 39 indicates identity
Score > 14 indicates homology
NPIQDIKITSDTNVPVNAGIR + 2 Deamidated (NQ)
3857 824.1067 2469.2983 2469.2976 0.29 1 6 0.54 +1Score > 36 indicates identity
Score > 16 indicates homology
FNALGLSTRDLVALSGAHTIGQAR + 2 Deamidated (NQ)
1663 638.8600 1275.7054 1275.7009 3.56 1 6 0.74 +1Score > 39 indicates identity
Score > 18 indicates homology
LMSKILGSFHK + Oxidation (M)
3832 816.7606 2447.2600 2447.2471 5.25 1 6 0.41 +1Score > 38 indicates identity
Score > 15 indicates homology
IKWGQYVTQNQILGINDVLIE + 4 Deamidated (NQ)
3224 699.3751 2095.1035 2095.1434 -19.1 1 6 0.75 +1Score > 38 indicates identity
Score > 18 indicates homology
RNTFLQLAMVLFAVVMAR + Oxidation (M)
3409 546.2830 2181.1029 2181.0810 10.1 1 6 0.93 +1Score > 39 indicates identity
Score > 19 indicates homology
KGNIVDGDQIMFIIGQAMSK + Deamidated (NQ); Oxidation (M)
3035 660.3173 1977.9301 1977.9322 -1.06 1 6 0.68 +1Score > 39 indicates identity
Score > 17 indicates homology
MANQGMGPIMVLNSKTQR + 3 Deamidated (NQ)
3432 1096.5682 2191.1218 2191.1062 7.15 0 6 0.68 +1Score > 39 indicates identity
Score > 17 indicates homology
SFLGQIPLYSSGYAADHPLR
3500 1112.0245 2222.0344 2222.0678 -15.0 1 6 0.95 +1Score > 39 indicates identity
Score > 19 indicates homology
QILPLFDQFTQDIAAECGR + Deamidated (NQ)
1570 410.9345 1229.7817 1229.7748 5.63 0 6 0.76 +1Score > 22 indicates identity
Score > 18 indicates homology
ILLQFIGVTVK
2111 749.3962 1496.7778 1496.7623 10.4 0 6 0.33 +1Score > 39 indicates identity
Score > 14 indicates homology
NSSLQGGYFILAAR + Deamidated (NQ)
2404 539.5975 1615.7707 1615.7842 -8.36 1 6 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
GTEQWLQQLQDLR + 2 Deamidated (NQ)
2033 728.4357 1454.8568 1454.8456 7.70 1 6 0.64 +1Score > 33 indicates identity
Score > 17 indicates homology
AITSPLAAGSKPSKK
2967 643.3603 1927.0591 1927.0639 -2.52 1 6 0.51 +1Score > 36 indicates identity
Score > 16 indicates homology
VFNDVRLLLNNDNLLR
3438 1097.0366 2192.0586 2192.0637 -2.31 0 6 0.94 +1Score > 39 indicates identity
Score > 19 indicates homology
LSDIYQYPGLDIALQPDDR + Deamidated (NQ)
4865 865.2504 4321.2156 4321.1610 12.6 1 6 0.53 +1Score > 31 indicates identity
Score > 16 indicates homology
ARVVFTSISMSKPHILLLDEPTNHLDMQSIDALADALDE + Oxidation (M)
3349 538.7957 2151.1537 2151.1436 4.67 1 6 0.28 +1Score > 36 indicates identity
Score > 13 indicates homology
GVHLGQALLENGIIHHVTDK + Deamidated (NQ)
523 767.4432 766.4359 766.4490 -17.0 1 6 0.55 +1Score > 37 indicates identity
Score > 16 indicates homology
KVAFFR
3107 675.0209 2022.0409 2022.0435 -1.30 1 6 0.42 +1Score > 39 indicates identity
Score > 15 indicates homology
IHSRPDPLWNFRNLQK + 2 Deamidated (NQ)
4287 728.1315 2908.4969 2908.5433 -16.0 0 6 1 +1Score > 36 indicates identity
Score > 19 indicates homology
VNVFPATTPTLPATTAILPLVNSGQNLR + 4 Deamidated (NQ)
1848 455.2502 1362.7288 1362.7256 2.36 1 6 0.82 +1Score > 40 indicates identity
Score > 18 indicates homology
TGVQLRVHINPE + Deamidated (NQ)
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Not what you expected? Try the select summary.