MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3075 1001.5375 2001.0604 2001.0419 9.29 1 8 0.81 +1Score > 39 indicates identity
Score > 19 indicates homology
QTGGTGQFARVIIAIEPIE + 2 Deamidated (NQ)
2229 517.6078 1549.8016 1549.7963 3.40 0 8 0.62 +1Score > 40 indicates identity
Score > 18 indicates homology
FGGSMTLHIVPFTK + Oxidation (M)
349 341.6987 681.3828 681.3922 -13.7 0 8 0.9 +1Score > 37 indicates identity
Score > 20 indicates homology
IGHVTR
2701 886.9523 1771.8900 1771.9005 -5.92 0 8 0.55 +1Score > 40 indicates identity
Score > 18 indicates homology
IFQLTEPGNRPASWR + Deamidated (NQ)
2800 609.3047 1824.8923 1824.9192 -14.8 1 8 0.38 +1Score > 40 indicates identity
Score > 16 indicates homology
AIMDKFGGAASGPTALFR + Oxidation (M)
3727 787.7609 2360.2609 2360.2416 8.16 0 8 0.56 +1Score > 36 indicates identity
Score > 18 indicates homology
QTLLAWLDALGWPAPEPGALLE
1910 465.8988 1394.6746 1394.6929 -13.1 0 8 0.82 +1Score > 41 indicates identity
Score > 19 indicates homology
NIVPILADANQPE + 2 Deamidated (NQ)
2661 585.6508 1753.9306 1753.8999 17.5 1 8 0.8 +1Score > 38 indicates identity
Score > 19 indicates homology
YFDLGVKVTLNTDNR
3965 516.4723 2577.3251 2577.2859 15.2 0 8 0.42 +1Score > 37 indicates identity
Score > 16 indicates homology
GLILTMGSFAGVMPTPYLATYSGSK + Oxidation (M)
1125 340.8636 1019.5690 1019.5499 18.7 1 8 0.83 +1Score > 39 indicates identity
Score > 19 indicates homology
SQTLSDKIK + Deamidated (NQ)
3076 1002.0374 2002.0602 2002.0735 -6.62 1 8 0.86 +1Score > 39 indicates identity
Score > 19 indicates homology
AGVVSDVGGIEQVNYILIR + Deamidated (NQ)
3826 813.7893 2438.3461 2438.3429 1.30 1 8 0.81 +1Score > 32 indicates identity
Score > 19 indicates homology
LQIMAHISLLVGAGVMSLLAKWA + Deamidated (NQ); Oxidation (M)
215 611.2883 610.2810 610.2785 4.18 0 8 0.77 +1Score > 37 indicates identity
Score > 19 indicates homology
ADFMK
888 463.2634 924.5122 924.5029 10.1 1 8 0.88 +1Score > 37 indicates identity
Score > 20 indicates homology
ELGPPSAVR
3193 520.2926 2077.1413 2077.1241 8.26 1 8 0.6 +1Score > 34 indicates identity
Score > 18 indicates homology
QMLAGLLKSLGGTLNFLQR + 2 Deamidated (NQ); Oxidation (M)
3213 697.7359 2090.1859 2090.1623 11.3 1 8 1 +1Score > 31 indicates identity
Score > 20 indicates homology
YIVNTVLDLGGQTTKILDK
3073 1000.5420 1999.0694 1999.0959 -13.2 0 8 0.85 +1Score > 37 indicates identity
Score > 19 indicates homology
VLALIGSITVMVMTVGHAR + 2 Oxidation (M)
2322 529.6296 1585.8670 1585.8464 13.0 0 8 0.71 +1Score > 38 indicates identity
Score > 19 indicates homology
VVVAYGGAPINQQLR + 2 Deamidated (NQ)
2712 889.4610 1776.9074 1776.8968 5.99 0 8 0.92 +1Score > 40 indicates identity
Score > 20 indicates homology
TVTISVDIPQAGFMIGE
3214 697.7383 2090.1931 2090.1623 14.7 1 8 0.25 +1Score > 30 indicates identity
Score > 14 indicates homology
YIVNTVLDLGGQTTKILDK
4201 705.8489 2819.3665 2819.3324 12.1 0 8 1 +1Score > 37 indicates identity
Score > 20 indicates homology
AQVSVDMFLFSSQYQDVASLSNLPR + 2 Deamidated (NQ); Oxidation (M)
1723 435.2617 1302.7633 1302.7408 17.3 1 8 0.65 +1Score > 34 indicates identity
Score > 18 indicates homology
AARLGLPVAVHAE
2058 735.4189 1468.8232 1468.7960 18.6 1 8 0.97 +1Score > 37 indicates identity
Score > 20 indicates homology
SKCVVLTTGTYLK
1897 463.9359 1388.7859 1388.7888 -2.11 1 8 0.58 +1Score > 36 indicates identity
Score > 18 indicates homology
AVRAATYGGALALR
2296 788.9174 1575.8202 1575.8257 -3.44 1 8 0.88 +1Score > 40 indicates identity
Score > 19 indicates homology
ETALFGGISGAVDLAR
3176 1030.5378 2059.0610 2059.0375 11.5 1 8 0.87 +1Score > 39 indicates identity
Score > 19 indicates homology
GDKVQGDGDWKPIVQFLR + 2 Deamidated (NQ)
3459 550.2759 2197.0745 2197.0666 3.58 0 8 0.76 +1Score > 39 indicates identity
Score > 19 indicates homology
HNFSVWNMLLYFNSALPK + Deamidated (NQ); Oxidation (M)
2668 587.3292 1758.9658 1758.9516 8.07 1 8 0.57 +1Score > 37 indicates identity
Score > 18 indicates homology
LKLGSQNASGVFPALQK + 2 Deamidated (NQ)
3671 775.3997 2323.1773 2323.1961 -8.09 1 7 0.65 +1Score > 38 indicates identity
Score > 18 indicates homology
TTGQAYWKPSSSWDLISLKR
2048 488.2866 1461.8380 1461.8231 10.1 1 7 0.82 +1Score > 34 indicates identity
Score > 19 indicates homology
YGPDGLPYLKVLK
4924 820.4246 4916.5039 4916.4304 15.0 0 7 0.42 +1Score > 29 indicates identity
Score > 16 indicates homology
QLFGADYANVQPHSGSSANGAVYLALLQAGDTILGMSLAHGGHLTHGAK + Deamidated (NQ)
1974 712.8330 1423.6514 1423.6328 13.1 1 7 0.82 +1Score > 40 indicates identity
Score > 19 indicates homology
NNEGVTNHTPVNE
2249 312.5897 1557.9121 1557.9283 -10.4 0 7 0.22 +1Score > 31 indicates identity
Score > 13 indicates homology
GLVLLIILQWFSR + Deamidated (NQ)
3121 679.0321 2034.0745 2034.0534 10.3 1 7 0.45 +1Score > 38 indicates identity
Score > 17 indicates homology
EAGPGPGPGPLNVGLHLNPLE
3417 729.3961 2185.1665 2185.1241 19.4 1 7 0.99 +1Score > 36 indicates identity
Score > 20 indicates homology
FPGKALADLAGKPMIQHVYE + Deamidated (NQ)
3820 812.7807 2435.3203 2435.2745 18.8 0 7 0.62 +1Score > 35 indicates identity
Score > 18 indicates homology
MLWVVMDDLLYIGALGAPFGVR
2808 919.0151 1836.0156 1836.0231 -4.03 0 7 0.61 +1Score > 35 indicates identity
Score > 18 indicates homology
ALLAQRPHALQAGHTPR
3285 709.7238 2126.1496 2126.1232 12.4 1 7 0.8 +1Score > 36 indicates identity
Score > 19 indicates homology
QANSGLTHFTAALIQLRQR + 2 Deamidated (NQ)
3669 774.3659 2320.0759 2320.1198 -18.9 1 7 1 +1Score > 38 indicates identity
Score > 20 indicates homology
FSRSILWDYFSLVLTNACE
343 338.2079 674.4012 674.3897 17.1 1 7 0.83 +1Score > 39 indicates identity
Score > 19 indicates homology
AVKAMR
2901 472.7564 1886.9965 1887.0101 -7.22 1 7 0.75 +1Score > 38 indicates identity
Score > 19 indicates homology
LLYLQQQAAQALELQR + 2 Deamidated (NQ)
2951 640.3428 1918.0066 1918.0233 -8.75 0 7 0.32 +1Score > 39 indicates identity
Score > 15 indicates homology
SALAMVVQALAPIAPHISE + Deamidated (NQ)
3631 1151.6361 2301.2576 2301.2540 1.60 1 7 0.22 +1Score > 35 indicates identity
Score > 13 indicates homology
SIGGSLTIKNTVTTAAIQLQQR + 2 Deamidated (NQ)
2389 538.6414 1612.9024 1612.9162 -8.54 1 7 0.5 +1Score > 36 indicates identity
Score > 17 indicates homology
TIVRSDPHIGLLHR
2817 369.1920 1840.9236 1840.8989 13.4 1 7 0.72 +1Score > 40 indicates identity
Score > 18 indicates homology
IDKICSIALSNGSYGSR + Deamidated (NQ)
3279 708.0302 2121.0688 2121.0702 -0.68 1 7 0.47 +1Score > 39 indicates identity
Score > 17 indicates homology
IGKDVNIGAGTITANYDGTNK
928 470.7946 939.5746 939.5641 11.3 0 7 0.9 +1Score > 35 indicates identity
Score > 19 indicates homology
LSLAVLPNI + Deamidated (NQ)
1884 693.8970 1385.7794 1385.8064 -19.5 1 7 0.67 +1Score > 37 indicates identity
Score > 18 indicates homology
IALTMNLLVRNK + Deamidated (NQ)
2531 558.6529 1672.9369 1672.9148 13.2 1 7 0.83 +1Score > 36 indicates identity
Score > 19 indicates homology
NTFIQSPKLLDGALR + Deamidated (NQ)
3039 660.6657 1978.9753 1979.0146 -19.9 1 7 0.9 +1Score > 40 indicates identity
Score > 19 indicates homology
SVGGMRASIYNAVDINAIK + Deamidated (NQ)
2267 392.4602 1565.8117 1565.8011 6.78 0 7 0.45 +1Score > 40 indicates identity
Score > 16 indicates homology
MSVNVASLDFLLIE + Oxidation (M)
2489 556.3031 1665.8875 1665.8726 8.93 1 7 0.7 +1Score > 38 indicates identity
Score > 18 indicates homology
SVTVYLVNENPYIR
2441 817.9392 1633.8638 1633.8576 3.80 1 7 0.45 +1Score > 39 indicates identity
Score > 16 indicates homology
HNPFIDLVPEQGIR
660 831.4351 830.4278 830.4134 17.4 1 7 0.24 +1Score > 44 indicates identity
Score > 14 indicates homology
AIGGAEQGK + Deamidated (NQ)
2433 408.7241 1630.8673 1630.8678 -0.33 0 7 0.99 +1Score > 39 indicates identity
Score > 20 indicates homology
INDGVAIALYGLNAAR + Deamidated (NQ)
3282 709.3810 2125.1212 2125.0837 17.6 1 7 0.47 +1Score > 37 indicates identity
Score > 17 indicates homology
TVGDMTIQRVAHLQLSNLE + Deamidated (NQ)
2252 520.6247 1558.8523 1558.8501 1.40 1 7 0.52 +1Score > 38 indicates identity
Score > 17 indicates homology
KMANLATSIGIQGQK
2765 603.3116 1806.9130 1806.9251 -6.72 1 7 0.43 +1Score > 40 indicates identity
Score > 16 indicates homology
SIGVSNFTEDLLATVIE
1969 474.9370 1421.7892 1421.7878 0.97 1 7 0.8 +1Score > 37 indicates identity
Score > 19 indicates homology
VQVLALEPGKNPR + 2 Deamidated (NQ)
2023 724.3799 1446.7452 1446.7579 -8.74 0 7 0.71 +1Score > 41 indicates identity
Score > 18 indicates homology
VYGNNAAALSQALR
788 443.7630 885.5114 885.5283 -19.1 1 7 0.78 +1Score > 41 indicates identity
Score > 19 indicates homology
LAKQQAVK + Deamidated (NQ)
2657 584.3147 1749.9223 1749.8944 15.9 1 7 0.65 +1Score > 39 indicates identity
Score > 18 indicates homology
SLTQRPGVYRMVDGR + Oxidation (M)
3028 658.0418 1971.1036 1971.0789 12.5 1 7 0.48 +1Score > 34 indicates identity
Score > 17 indicates homology
AVSVGLLGNAADVFAELVAR
4247 959.8365 2876.4877 2876.4778 3.43 0 7 0.33 +1Score > 36 indicates identity
Score > 15 indicates homology
TIGNIVLVTMLLDFMFACIGVQLFK + 2 Deamidated (NQ); 2 Oxidation (M)
2481 830.9276 1659.8406 1659.8203 12.3 1 7 0.91 +1Score > 41 indicates identity
Score > 19 indicates homology
ALVQQTLEQLVNSSE + 2 Deamidated (NQ)
4391 763.4368 3049.7181 3049.7613 -14.2 0 7 1 +1Score > 28 indicates identity
Score > 20 indicates homology
VLAAMTLLGIPAAVLVALAAQLLFQLQAGR + 2 Deamidated (NQ); Oxidation (M)
2594 856.9989 1711.9832 1711.9872 -2.33 0 7 0.33 +1Score > 34 indicates identity
Score > 15 indicates homology
ILGIFGAGQVLLKPASE
3891 835.4525 2503.3357 2503.2958 15.9 1 7 0.59 +1Score > 35 indicates identity
Score > 17 indicates homology
ADTTIVVKSYRPAQLTWSQLPE + Deamidated (NQ)
3689 584.7877 2335.1217 2335.1339 -5.22 1 7 0.88 +1Score > 39 indicates identity
Score > 19 indicates homology
NASKGPHSPQVPSQVQSPMTSR + Oxidation (M)
3742 475.4758 2372.3426 2372.3315 4.69 1 7 0.43 +1Score > 30 indicates identity
Score > 16 indicates homology
KSATLVSNSQFLLKPLDNIVGK + Deamidated (NQ)
3380 1086.1495 2170.2844 2170.2911 -3.08 0 7 0.54 +1Score > 26 indicates identity
Score > 17 indicates homology
LIIFAMNGVLVAIAGLILSAR + Oxidation (M)
2067 738.4003 1474.7860 1474.7667 13.1 1 7 0.98 +1Score > 40 indicates identity
Score > 20 indicates homology
KQVDYILGQNPAK + 2 Deamidated (NQ)
2484 417.2341 1664.9073 1664.8846 13.7 1 7 0.87 +1Score > 37 indicates identity
Score > 19 indicates homology
AVPAQDNRGNAIIPTK + Deamidated (NQ)
3024 986.0330 1970.0514 1970.0797 -14.3 0 7 0.9 +1Score > 37 indicates identity
Score > 19 indicates homology
AATDLGVTTLLIAGGVAANSR
3598 570.2990 2277.1669 2277.1345 14.2 0 7 0.33 +1Score > 38 indicates identity
Score > 15 indicates homology
GADMVIITSGMGGGTGTGASPIIAK + Oxidation (M)
2918 633.6605 1897.9597 1897.9269 17.3 0 7 0.55 +1Score > 39 indicates identity
Score > 17 indicates homology
LDDVNDVDQQALLPSVR + 2 Deamidated (NQ)
4505 1064.5544 3190.6414 3190.6411 0.086 1 7 0.5 +1Score > 35 indicates identity
Score > 17 indicates homology
WTRNSQGVQLTNPTPYYINLIQVSVNGK + Deamidated (NQ)
159 554.2963 553.2890 553.2860 5.45 0 7 0.8 +1Score > 41 indicates identity
Score > 19 indicates homology
ADLHV
1673 427.5645 1279.6717 1279.6772 -4.28 1 7 0.55 +1Score > 40 indicates identity
Score > 17 indicates homology
LKNPISLNHNK + 3 Deamidated (NQ)
3332 1073.5768 2145.1390 2145.1252 6.46 0 7 0.6 +1Score > 37 indicates identity
Score > 17 indicates homology
CKPSAPQTLHQILQDPALK + Deamidated (NQ)
2675 588.6555 1762.9447 1762.9519 -4.09 0 7 0.65 +1Score > 38 indicates identity
Score > 18 indicates homology
FPVQYVLRPNLDFR
2795 608.9915 1823.9527 1823.9417 5.99 1 7 0.38 +1Score > 39 indicates identity
Score > 15 indicates homology
TAQKLAFQAAVSDAFQK + Deamidated (NQ)
3146 682.3610 2044.0612 2044.0854 -11.8 1 7 0.56 +1Score > 38 indicates identity
Score > 17 indicates homology
LALPFGIALHHAGLTENDR
3649 578.2965 2309.1569 2309.1209 15.6 1 7 0.83 +1Score > 39 indicates identity
Score > 19 indicates homology
MTAQNVRQSIVNAVYANIGLE + 3 Deamidated (NQ); Oxidation (M)
2260 781.8836 1561.7526 1561.7334 12.3 1 7 0.61 +1Score > 41 indicates identity
Score > 18 indicates homology
YMAATSKTQGLIFD + Deamidated (NQ); Oxidation (M)
2413 810.4577 1618.9008 1618.8752 15.8 0 7 0.24 +1Score > 36 indicates identity
Score > 14 indicates homology
IAPTQAMIVPIAQHK + 2 Deamidated (NQ)
2834 927.9588 1853.9030 1853.9049 -0.99 1 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
ASVRIMNGMIATMSINK + 2 Deamidated (NQ); Oxidation (M)
2999 652.3486 1954.0240 1954.0016 11.5 1 7 0.83 +1Score > 38 indicates identity
Score > 19 indicates homology
HAMIENLMTILAITPDR + Oxidation (M)
585 792.4139 791.4066 791.4025 5.24 1 7 0.91 +1Score > 42 indicates identity
Score > 19 indicates homology
QLSKTSE
974 958.5266 957.5193 957.5382 -19.8 1 7 0.55 +1Score > 42 indicates identity
Score > 17 indicates homology
QVIEQLVK + 2 Deamidated (NQ)
3275 707.7198 2120.1376 2120.0976 18.9 0 7 0.52 +1Score > 36 indicates identity
Score > 17 indicates homology
GSPSRPYFVTPALMNILLE + Oxidation (M)
3804 807.7284 2420.1634 2420.1295 14.0 1 7 0.39 +1Score > 39 indicates identity
Score > 16 indicates homology
MVMIACQLKPHYCCLVPER + Oxidation (M)
3463 733.3969 2197.1689 2197.1703 -0.63 1 7 0.53 +1Score > 37 indicates identity
Score > 17 indicates homology
NTLGDIGNQVSAITISDVPRK
3632 768.0945 2301.2617 2301.2249 16.0 1 7 0.43 +1Score > 34 indicates identity
Score > 16 indicates homology
ALMGQIAGALNSITSKIAIGINE + Deamidated (NQ); Oxidation (M)
3056 663.6539 1987.9399 1987.9673 -13.8 1 7 0.84 +1Score > 39 indicates identity
Score > 19 indicates homology
AGMTKQALGLYASNYNLR + 2 Deamidated (NQ); Oxidation (M)
2399 807.9775 1613.9404 1613.9327 4.81 1 7 0.99 +1Score > 32 indicates identity
Score > 20 indicates homology
QAKIAFLMLPGLGVR + Deamidated (NQ)
4076 901.8038 2702.3896 2702.3360 19.8 1 7 0.57 +1Score > 37 indicates identity
Score > 17 indicates homology
SIVADSWLAAMQDVLKVAVQSDIPE + 2 Deamidated (NQ); Oxidation (M)
4142 924.8150 2771.4232 2771.4666 -15.7 1 7 0.65 +1Score > 36 indicates identity
Score > 18 indicates homology
QIVSMDLINKLADVIITNSYITYK + Deamidated (NQ); Oxidation (M)
1807 672.4099 1342.8052 1342.7932 8.96 1 7 0.85 +1Score > 32 indicates identity
Score > 19 indicates homology
SIGSSLAKAAIAVR
2301 527.3046 1578.8920 1578.8664 16.2 0 7 0.89 +1Score > 35 indicates identity
Score > 19 indicates homology
GIMLVASGNVVHNLR
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