MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2906 944.5159 1887.0172 1886.9845 17.3 1 9 0.38 +1Score > 37 indicates identity
Score > 17 indicates homology
KNAVVMMGAVINIGAVIGE + 2 Deamidated (NQ)
3027 658.0002 1970.9788 1971.0021 -11.8 1 9 0.61 +1Score > 40 indicates identity
Score > 20 indicates homology
SLQQALQQAQQIAQTRR + 4 Deamidated (NQ)
648 413.7570 825.4994 825.4960 4.21 1 9 0.88 +1Score > 35 indicates identity
Score > 21 indicates homology
IPENILK
2541 561.9853 1682.9341 1682.9468 -7.56 1 9 0.26 +1Score > 36 indicates identity
Score > 16 indicates homology
TGAFALLDSIVRHGVK
3666 773.4532 2317.3378 2317.3270 4.64 1 9 0.19 +1Score > 28 indicates identity
Score > 14 indicates homology
ILNIPKLGSVNVHGSILPQYR
1417 389.5576 1165.6510 1165.6343 14.3 1 9 0.84 +1Score > 37 indicates identity
Score > 21 indicates homology
QLGIPDPEGIK
3348 1076.5609 2151.1072 2151.0848 10.5 1 9 0.74 +1Score > 38 indicates identity
Score > 20 indicates homology
KPHTTTVAPPQINFIKNNE + 3 Deamidated (NQ)
2760 602.3055 1803.8947 1803.9011 -3.58 1 9 0.81 +1Score > 40 indicates identity
Score > 21 indicates homology
AIDMLSTFIKPMHRE + Oxidation (M)
1793 668.8308 1335.6470 1335.6307 12.3 0 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
VVSSTFDEPAQR + Deamidated (NQ)
3841 818.7824 2453.3254 2453.3181 2.96 0 9 0.42 +1Score > 35 indicates identity
Score > 18 indicates homology
FFLLMAVIYTTLAIAPVHFDR + Oxidation (M)
2142 755.4064 1508.7982 1508.7909 4.89 1 9 0.64 +1Score > 39 indicates identity
Score > 20 indicates homology
GKIVIDVTSMGFNK + Deamidated (NQ)
3614 765.4020 2293.1842 2293.1889 -2.04 1 9 0.71 +1Score > 38 indicates identity
Score > 20 indicates homology
WTLPSPLNSGLDLANKMPSPR
4359 1004.1868 3009.5386 3009.5382 0.14 1 9 0.26 +1Score > 36 indicates identity
Score > 16 indicates homology
AWLRMFLNNGSISYLVIRPNGNVSLR + 4 Deamidated (NQ); Oxidation (M)
728 859.4344 858.4271 858.4307 -4.21 1 9 0.75 +1Score > 41 indicates identity
Score > 20 indicates homology
GNLNRQR + 2 Deamidated (NQ)
1707 432.9160 1295.7262 1295.7193 5.34 1 9 0.73 +1Score > 37 indicates identity
Score > 20 indicates homology
MSMLSTLLLKK + 2 Oxidation (M)
3043 990.9642 1979.9138 1979.8782 18.0 0 9 0.5 +1Score > 39 indicates identity
Score > 19 indicates homology
QASSQSMPWLADLVQSSE + Deamidated (NQ); Oxidation (M)
642 412.2337 822.4528 822.4422 13.0 0 9 0.26 +1Score > 38 indicates identity
Score > 16 indicates homology
FMVSALR
3097 1010.0084 2018.0022 2018.0216 -9.61 1 9 0.94 +1Score > 40 indicates identity
Score > 21 indicates homology
LESIVMNWLGQMLNLPK + Deamidated (NQ); 2 Oxidation (M)
3485 737.3879 2209.1419 2209.0990 19.4 1 9 0.41 +1Score > 38 indicates identity
Score > 18 indicates homology
ISMVFQHFNLLWSRTVSE + Oxidation (M)
2523 836.9456 1671.8766 1671.8693 4.41 0 9 0.87 +1Score > 40 indicates identity
Score > 21 indicates homology
IGTVTPNPQPGNPQPR
3239 1051.5291 2101.0436 2101.0375 2.95 1 9 0.75 +1Score > 40 indicates identity
Score > 20 indicates homology
NAGKAVHIMGLLSAGGVHSHE + Deamidated (NQ); Oxidation (M)
1838 453.5786 1357.7140 1357.7241 -7.48 1 9 0.61 +1Score > 41 indicates identity
Score > 19 indicates homology
NNWLASVLLEAK + Deamidated (NQ)
1958 710.3851 1418.7556 1418.7769 -15.0 1 9 0.69 +1Score > 40 indicates identity
Score > 20 indicates homology
DKSNVLQLQFVK + Deamidated (NQ)
2216 515.2891 1542.8455 1542.8617 -10.5 1 9 0.7 +1Score > 38 indicates identity
Score > 20 indicates homology
SILSDLEGTAIVAVR
2622 576.6491 1726.9255 1726.8964 16.9 0 9 0.69 +1Score > 38 indicates identity
Score > 20 indicates homology
LMLNAVNLTFIAFSR + 2 Deamidated (NQ); Oxidation (M)
2883 627.3398 1878.9976 1878.9799 9.38 0 9 0.99 +1Score > 38 indicates identity
Score > 21 indicates homology
NQTVDLVLTAHPTQSVR + Deamidated (NQ)
3038 990.4914 1978.9682 1978.9669 0.66 1 9 0.7 +1Score > 40 indicates identity
Score > 20 indicates homology
TLNALAAMGVYQDGIAKNE + Deamidated (NQ)
1259 365.2101 1092.6085 1092.6179 -8.63 1 9 0.72 +1Score > 38 indicates identity
Score > 20 indicates homology
KLGSIVFSDK
3695 780.7182 2339.1328 2339.1254 3.14 0 9 0.61 +1Score > 39 indicates identity
Score > 19 indicates homology
SPHDAFGTGHASTSISAAAGIASAR
1350 380.2010 1137.5812 1137.5891 -6.93 0 9 0.52 +1Score > 41 indicates identity
Score > 19 indicates homology
AGLSLQQQHR + Deamidated (NQ)
1787 667.3623 1332.7100 1332.6925 13.2 0 9 0.95 +1Score > 40 indicates identity
Score > 21 indicates homology
LLDAAGYQALIGE
1909 697.8852 1393.7558 1393.7718 -11.4 1 9 0.6 +1Score > 38 indicates identity
Score > 19 indicates homology
NIAVSVFVFDRK
4013 878.4575 2632.3507 2632.3101 15.4 1 9 0.33 +1Score > 37 indicates identity
Score > 17 indicates homology
QSGVNPIVVHGGGPQIGAMLNRMGIE + 2 Deamidated (NQ)
2497 834.4644 1666.9142 1666.9083 3.58 0 9 0.19 +1Score > 37 indicates identity
Score > 14 indicates homology
LLINGGTISPFFFNK
1348 379.8904 1136.6494 1136.6442 4.59 0 9 0.66 +1Score > 36 indicates identity
Score > 20 indicates homology
TGTTIVGVVYK
1977 475.9117 1424.7133 1424.7187 -3.82 1 9 0.69 +1Score > 41 indicates identity
Score > 20 indicates homology
AKFLAVFASNLDE + Deamidated (NQ)
2596 429.4652 1713.8317 1713.8356 -2.26 1 9 0.68 +1Score > 40 indicates identity
Score > 20 indicates homology
VTLIPCGDGNAVAAARE + Deamidated (NQ)
968 479.2589 956.5032 956.4974 6.11 1 9 0.47 +1Score > 40 indicates identity
Score > 18 indicates homology
QIHMRTR + Oxidation (M)
1583 618.3179 1234.6212 1234.6275 -5.03 1 9 0.89 +1Score > 42 indicates identity
Score > 21 indicates homology
RGVCLQVMTR + Oxidation (M)
2879 470.5069 1877.9985 1878.0172 -9.97 1 9 0.61 +1Score > 38 indicates identity
Score > 19 indicates homology
IVEVLGIPPAAMLDQAPK + Deamidated (NQ); Oxidation (M)
3654 771.0602 2310.1588 2310.1638 -2.16 1 9 0.33 +1Score > 39 indicates identity
Score > 17 indicates homology
QGVMLLNRVLTVQPGNSNSHK + 3 Deamidated (NQ); Oxidation (M)
1611 415.5482 1243.6228 1243.6231 -0.23 1 9 0.58 +1Score > 41 indicates identity
Score > 19 indicates homology
QGGRIGMVIPSE + Deamidated (NQ)
4704 907.4594 3625.8085 3625.8621 -14.8 1 9 1 +1Score > 34 indicates identity
Score > 21 indicates homology
MSLNQAASVNNLVLSPNTTLKDVALNALAGQVDAR + 2 Deamidated (NQ); Oxidation (M)
3098 1010.0540 2018.0934 2018.1016 -4.06 1 9 0.99 +1Score > 36 indicates identity
Score > 21 indicates homology
LLMTLTNDIICRITLGR + Oxidation (M)
3670 774.4185 2320.2337 2320.1924 17.8 1 9 0.58 +1Score > 36 indicates identity
Score > 19 indicates homology
NFTRITSPNYRPTQQDILR + Deamidated (NQ)
3416 546.7987 2183.1657 2183.2062 -18.6 1 9 0.86 +1Score > 37 indicates identity
Score > 21 indicates homology
NYGPLPIGSNARSILLTLQR + Deamidated (NQ)
1433 391.5695 1171.6867 1171.6673 16.5 1 9 0.96 +1Score > 37 indicates identity
Score > 21 indicates homology
ANTGGLSKLVGR
2813 920.4421 1838.8696 1838.8655 2.25 1 9 0.93 +1Score > 40 indicates identity
Score > 21 indicates homology
RVCAVIGDGALSAGMAFE + Oxidation (M)
2890 941.5046 1880.9946 1881.0108 -8.60 1 9 0.81 +1Score > 38 indicates identity
Score > 20 indicates homology
AKPEFLHTLNGSGLAIGR + Deamidated (NQ)
836 452.7638 903.5130 903.5099 3.46 0 9 0.96 +1Score > 39 indicates identity
Score > 21 indicates homology
AMIIVVNK + Deamidated (NQ); Oxidation (M)
4715 735.3848 3671.8876 3671.8617 7.05 1 9 1 +1Score > 33 indicates identity
Score > 21 indicates homology
MSQGGGLNILSIQSSVAYGHVGNSAAVFPLQRLGIE + 2 Deamidated (NQ)
2841 928.4946 1854.9746 1854.9475 14.6 1 9 0.15 +1Score > 38 indicates identity
Score > 13 indicates homology
QLLADGGHAILAEVYQR + 2 Deamidated (NQ)
1260 365.5258 1093.5556 1093.5624 -6.22 0 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
MTMLSAAVVR + Oxidation (M)
2639 578.6555 1732.9447 1732.9512 -3.76 1 9 0.56 +1Score > 37 indicates identity
Score > 19 indicates homology
FEPLIKVLGLNPHAGE
2909 946.4816 1890.9486 1890.9218 14.2 1 9 0.53 +1Score > 40 indicates identity
Score > 19 indicates homology
DDVAASMALRIAGSSGQAR + Oxidation (M)
3511 744.3741 2230.1005 2230.1067 -2.82 1 9 0.32 +1Score > 39 indicates identity
Score > 16 indicates homology
AGVKVICYNFMPVFDWLR + Oxidation (M)
3677 775.4287 2323.2643 2323.2466 7.61 1 9 0.31 +1Score > 34 indicates identity
Score > 16 indicates homology
RLMGLVLIMLSTQMFLDGIR + Deamidated (NQ); Oxidation (M)
2849 930.9733 1859.9320 1859.9014 16.5 1 9 0.34 +1Score > 40 indicates identity
Score > 17 indicates homology
APGQDVTLFRSPDSVDR + Deamidated (NQ)
3247 704.3702 2110.0888 2110.0921 -1.58 1 9 0.66 +1Score > 38 indicates identity
Score > 19 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
2092 496.9526 1487.8360 1487.8460 -6.74 1 9 0.92 +1Score > 36 indicates identity
Score > 21 indicates homology
LVQFINNRVSAVK + Deamidated (NQ)
2565 567.3216 1698.9430 1698.9450 -1.22 1 9 0.49 +1Score > 36 indicates identity
Score > 18 indicates homology
QAIQIIVKMAAANGVR + Deamidated (NQ); Oxidation (M)
2625 864.9768 1727.9390 1727.9193 11.4 1 9 0.9 +1Score > 38 indicates identity
Score > 21 indicates homology
TGVGIDSLLEAVLLQAE + Deamidated (NQ)
1514 606.3220 1210.6294 1210.6194 8.31 0 9 0.23 +1Score > 39 indicates identity
Score > 15 indicates homology
SVDVLIVHGGSE
3626 767.7566 2300.2480 2300.2224 11.1 1 9 0.31 +1Score > 35 indicates identity
Score > 16 indicates homology
VAVVTGASSGIGASIAETLANQGVK + Deamidated (NQ)
2511 557.3365 1668.9877 1668.9563 18.8 1 9 0.7 +1Score > 30 indicates identity
Score > 20 indicates homology
SRLPLLDLNIYVPR + Deamidated (NQ)
1717 651.3903 1300.7660 1300.7755 -7.23 1 9 0.93 +1Score > 34 indicates identity
Score > 21 indicates homology
LQPYVLKVVNK + Deamidated (NQ)
2305 790.9684 1579.9222 1579.9297 -4.73 0 9 0.74 +1Score > 33 indicates identity
Score > 20 indicates homology
VASLPLLLVSQANQK
2454 820.9214 1639.8282 1639.8062 13.5 1 9 0.67 +1Score > 40 indicates identity
Score > 19 indicates homology
ANIMRMSDGLFLQK + Deamidated (NQ); Oxidation (M)
2863 934.5257 1867.0368 1867.0091 14.9 1 9 0.63 +1Score > 35 indicates identity
Score > 19 indicates homology
QILDTLQKHFAVDLPK + 2 Deamidated (NQ)
1936 469.5999 1405.7779 1405.7599 12.8 1 9 0.99 +1Score > 37 indicates identity
Score > 21 indicates homology
MATITGQKALVTR + Deamidated (NQ); Oxidation (M)
3514 744.3969 2230.1689 2230.1667 0.96 0 9 0.26 +1Score > 37 indicates identity
Score > 15 indicates homology
MAAVILPSTAAPSSLFPASQQK + Oxidation (M)
2652 581.6703 1741.9891 1741.9614 15.9 0 9 0.64 +1Score > 34 indicates identity
Score > 19 indicates homology
TAIVDPFAQIPTLSIR + Deamidated (NQ)
3589 758.7428 2273.2066 2273.2267 -8.83 1 9 0.44 +1Score > 37 indicates identity
Score > 18 indicates homology
QYAALRGNVNLLGQLLGQTIK + 4 Deamidated (NQ)
3625 767.7552 2300.2438 2300.2488 -2.20 1 9 0.26 +1Score > 35 indicates identity
Score > 15 indicates homology
GEITGSTAGLAPGHVQANLAVLPK
1313 375.8660 1124.5762 1124.5978 -19.2 0 9 0.95 +1Score > 40 indicates identity
Score > 21 indicates homology
ASYLQKPYR
2786 606.6300 1816.8682 1816.8640 2.28 0 9 0.76 +1Score > 40 indicates identity
Score > 20 indicates homology
NYIHMNVFLSFMLR + Deamidated (NQ); 2 Oxidation (M)
2495 834.4525 1666.8904 1666.9115 -12.6 1 9 0.91 +1Score > 38 indicates identity
Score > 21 indicates homology
HRIDAVIATNTTISR
926 940.5807 939.5734 939.5865 -13.9 0 9 0.69 +1Score > 35 indicates identity
Score > 20 indicates homology
LLAAAIAAAR
3006 490.5042 1957.9877 1957.9601 14.1 1 9 0.96 +1Score > 40 indicates identity
Score > 21 indicates homology
ITNNMTTGELHDILMVR + Deamidated (NQ)
3599 1139.5920 2277.1694 2277.1457 10.4 0 9 0.62 +1Score > 38 indicates identity
Score > 19 indicates homology
MSAIQGIQGVISQLQATAMSAR + Deamidated (NQ); Oxidation (M)
1525 607.3370 1212.6594 1212.6462 10.9 1 9 1 +1Score > 40 indicates identity
Score > 21 indicates homology
HSDSVLAGTKAK
1266 549.2792 1096.5438 1096.5513 -6.75 1 9 0.92 +1Score > 40 indicates identity
Score > 21 indicates homology
ENAPVPNSLR + Deamidated (NQ)
2233 388.9742 1551.8677 1551.8695 -1.14 0 9 0.94 +1Score > 36 indicates identity
Score > 21 indicates homology
LTGVYVLATTLMVR + Oxidation (M)
3456 1099.5413 2197.0680 2197.0474 9.41 1 9 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
VDTEGVPFTSNVAANINYMR
3896 837.1086 2508.3040 2508.3258 -8.69 1 9 0.53 +1Score > 37 indicates identity
Score > 18 indicates homology
DIALAQGAVLVKSCQVPDGSIPIR + 2 Deamidated (NQ)
2705 591.6497 1771.9273 1771.9250 1.25 1 9 0.4 +1Score > 39 indicates identity
Score > 17 indicates homology
VNVLRLNMALDAAGATQ + Oxidation (M)
929 314.8369 941.4889 941.4971 -8.69 1 9 0.92 +1Score > 40 indicates identity
Score > 21 indicates homology
YKDIFTR
2651 581.6692 1741.9858 1741.9938 -4.60 1 9 0.94 +1Score > 34 indicates identity
Score > 21 indicates homology
QLAGLLLRITDGTVSGK + Deamidated (NQ)
3145 682.3586 2044.0540 2044.0337 9.90 1 9 0.96 +1Score > 39 indicates identity
Score > 21 indicates homology
RAAQLAGDIAYQEPDAVTR
2919 634.0175 1899.0307 1899.0023 15.0 1 9 0.19 +1Score > 37 indicates identity
Score > 14 indicates homology
LVQQAIGDQLAKMLLAGE + 2 Deamidated (NQ)
645 412.7743 823.5340 823.5280 7.40 0 9 0.81 +1Score > 28 indicates identity
Score > 20 indicates homology
IPVNLIR
2658 876.4321 1750.8496 1750.8573 -4.37 1 9 0.89 +1Score > 41 indicates identity
Score > 21 indicates homology
ARFHDAMNILGPQHK + Deamidated (NQ); Oxidation (M)
2945 959.0082 1916.0018 1915.9673 18.0 1 9 0.44 +1Score > 39 indicates identity
Score > 17 indicates homology
VSIGSQELQIVCGANNVK + Deamidated (NQ)
2005 479.9456 1436.8150 1436.8351 -14.0 1 9 0.91 +1Score > 35 indicates identity
Score > 21 indicates homology
EALQPLASLQVLR
3709 1175.5802 2349.1458 2349.1488 -1.26 1 9 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
HVYIVSQNFVNNQLVISSER + 4 Deamidated (NQ)
602 400.2443 798.4740 798.4712 3.60 1 9 0.69 +1Score > 38 indicates identity
Score > 19 indicates homology
RIGTAPGK
1990 715.8727 1429.7308 1429.7413 -7.31 1 9 0.58 +1Score > 41 indicates identity
Score > 19 indicates homology
VTGKTTAPSTGPGGSL
3067 999.4961 1996.9776 1996.9850 -3.66 1 9 0.49 +1Score > 40 indicates identity
Score > 18 indicates homology
LTLITPAMSVGSKLDCFE + Oxidation (M)
2020 482.6026 1444.7860 1444.8051 -13.3 1 9 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
LRVLHGHSNWVK
2050 488.2884 1461.8434 1461.8555 -8.28 1 9 0.73 +1Score > 34 indicates identity
Score > 20 indicates homology
GINLSPLHQKLLK + 2 Deamidated (NQ)
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