MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2994 975.5182 1949.0218 1948.9934 14.6 1 10 0.63 +1Score > 38 indicates identity
Score > 20 indicates homology
LAGGFNIADLFPSAKWLE + Deamidated (NQ)
1870 459.9143 1376.7211 1376.6969 17.5 0 10 0.85 +1Score > 40 indicates identity
Score > 22 indicates homology
NITNDILSIMAR + Deamidated (NQ); Oxidation (M)
1721 434.9120 1301.7142 1301.7303 -12.4 1 10 0.77 +1Score > 40 indicates identity
Score > 21 indicates homology
VVRILAGSTDGSK
2516 835.5038 1668.9930 1668.9662 16.1 1 10 0.51 +1Score > 29 indicates identity
Score > 19 indicates homology
DLLLIQGAGTISEIVK
2987 971.5423 1941.0700 1941.0935 -12.1 0 10 0.61 +1Score > 36 indicates identity
Score > 20 indicates homology
LLIVDRPIDQIAPVIHE + Deamidated (NQ)
3748 793.4059 2377.1959 2377.1658 12.7 0 10 0.2 +1Score > 38 indicates identity
Score > 15 indicates homology
TSYTQSLAVMSMVGYILGLGDR + Oxidation (M)
3294 1065.0621 2128.1096 2128.1020 3.58 0 10 0.45 +1Score > 38 indicates identity
Score > 19 indicates homology
AAGVINNVVASVPMVVNAVMR + 2 Deamidated (NQ); Oxidation (M)
2555 847.9278 1693.8410 1693.8199 12.5 1 10 0.42 +1Score > 41 indicates identity
Score > 19 indicates homology
QAWSSISALLDEFVE
2588 855.9510 1709.8874 1709.8584 17.0 0 10 0.87 +1Score > 39 indicates identity
Score > 22 indicates homology
DDGAVALDTLHQSGALK
3085 1005.9941 2009.9736 2009.9919 -9.06 1 10 0.73 +1Score > 40 indicates identity
Score > 21 indicates homology
HTIQLHVPHLENSAQQR + 3 Deamidated (NQ)
3594 1138.6537 2275.2928 2275.2940 -0.51 0 10 0.5 +1Score > 31 indicates identity
Score > 19 indicates homology
LPVGYVQLPVGIAGPLLLDGQR + Deamidated (NQ)
187 597.3420 596.3347 596.3282 10.9 0 10 0.41 +1Score > 37 indicates identity
Score > 18 indicates homology
EPPVR
3384 724.7313 2171.1721 2171.2062 -15.7 1 10 0.54 +1Score > 36 indicates identity
Score > 20 indicates homology
SGFRPLRATGSALNIASLVLE
3653 1155.6610 2309.3074 2309.2638 18.9 1 10 0.54 +1Score > 30 indicates identity
Score > 20 indicates homology
MIPVQGVQNQTIAVLGLGRSGR + Oxidation (M)
2228 775.9080 1549.8014 1549.7810 13.2 1 10 0.88 +1Score > 40 indicates identity
Score > 22 indicates homology
ALIEDPHAIPMTNK + Deamidated (NQ)
2827 925.4554 1848.8962 1848.9026 -3.45 1 10 0.6 +1Score > 40 indicates identity
Score > 20 indicates homology
GQLSSVTAVLQEMNLLE + 2 Deamidated (NQ); Oxidation (M)
2614 862.4719 1722.9292 1722.9457 -9.55 1 10 0.9 +1Score > 38 indicates identity
Score > 22 indicates homology
LFIATKDFNISLWR
3745 791.7902 2372.3488 2372.3290 8.33 1 10 0.66 +1Score > 30 indicates identity
Score > 20 indicates homology
ILGFMVLKDTQVLVNVWAIGR + Deamidated (NQ)
2226 387.9571 1547.7993 1547.8130 -8.85 0 10 0.87 +1Score > 41 indicates identity
Score > 22 indicates homology
ATNCLVVFINQIR + Deamidated (NQ)
2410 809.4719 1616.9292 1616.9324 -1.93 1 10 0.61 +1Score > 33 indicates identity
Score > 20 indicates homology
AVKIAQSLGFPVMLK + Oxidation (M)
708 427.7421 853.4696 853.4657 4.59 0 10 0.94 +1Score > 37 indicates identity
Score > 22 indicates homology
APAAAPAASK
1900 464.2603 1389.7591 1389.7537 3.85 1 10 0.91 +1Score > 39 indicates identity
Score > 22 indicates homology
MSILTQEALLVR + Deamidated (NQ); Oxidation (M)
3184 1038.5682 2075.1218 2075.1626 -19.6 1 10 0.34 +1Score > 36 indicates identity
Score > 18 indicates homology
NAIAELPADPVQAIGLIITR + Deamidated (NQ)
2169 759.3973 1516.7800 1516.7773 1.80 0 10 0.47 +1Score > 41 indicates identity
Score > 19 indicates homology
YIAQALPQDVLGTE
4256 722.3867 2885.5177 2885.5298 -4.19 0 10 0.14 +1Score > 35 indicates identity
Score > 14 indicates homology
IIYLMFFMVITMAFIGSVIFGVTTR + Oxidation (M)
3831 816.0836 2445.2290 2445.2627 -13.8 1 10 0.37 +1Score > 38 indicates identity
Score > 18 indicates homology
IAGALTYMHSAASFPIFHRDIK
4249 961.1332 2880.3778 2880.3409 12.8 1 10 0.25 +1Score > 37 indicates identity
Score > 16 indicates homology
HMDQLQQNVSIVTQQKMQPIYSVK + 6 Deamidated (NQ); 2 Oxidation (M)
230 617.3154 616.3081 616.3068 2.16 0 10 0.35 +1Score > 45 indicates identity
Score > 18 indicates homology
AITSPE
2915 632.0166 1893.0280 1892.9964 16.7 1 10 0.86 +1Score > 36 indicates identity
Score > 22 indicates homology
AMLGQSFKLQIMAANVR + Oxidation (M)
1424 390.5389 1168.5949 1168.5975 -2.28 1 10 0.99 +1Score > 40 indicates identity
Score > 22 indicates homology
AASPEPLIKDE
2248 520.2878 1557.8416 1557.8614 -12.7 1 10 0.26 +1Score > 39 indicates identity
Score > 16 indicates homology
ALETNTTLVVLDIR + Deamidated (NQ)
3231 525.2565 2096.9969 2097.0300 -15.8 0 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
FTAQVISISGDSVQLMDLR + 2 Deamidated (NQ); Oxidation (M)
2860 934.5058 1866.9970 1867.0051 -4.32 1 10 0.67 +1Score > 37 indicates identity
Score > 20 indicates homology
DTSLKLPPGVNGTVVDVR + Deamidated (NQ)
2492 834.4437 1666.8728 1666.8777 -2.94 0 10 0.42 +1Score > 39 indicates identity
Score > 18 indicates homology
AASDLGNPDLVILPGSK + Deamidated (NQ)
3320 1068.6072 2135.1998 2135.1739 12.2 1 10 0.66 +1Score > 32 indicates identity
Score > 20 indicates homology
WLPSLNPLDINVVVTGKDR
4164 698.3474 2789.3605 2789.3640 -1.27 1 10 1 +1Score > 37 indicates identity
Score > 22 indicates homology
VGSAAIDGVMQGAIQSIIQGENLGDSIK + 3 Deamidated (NQ); Oxidation (M)
2340 533.3018 1596.8836 1596.8597 15.0 1 10 0.62 +1Score > 37 indicates identity
Score > 20 indicates homology
GQRVHAVAGIAHPQR + Deamidated (NQ)
3072 667.3321 1998.9745 1998.9721 1.21 1 10 0.84 +1Score > 40 indicates identity
Score > 21 indicates homology
LLGGHMLDQFLPVRQQE + 3 Deamidated (NQ); Oxidation (M)
2477 414.9812 1655.8957 1655.9028 -4.30 1 10 0.95 +1Score > 39 indicates identity
Score > 22 indicates homology
MKNNIINLAQDLIR + Deamidated (NQ)
1240 362.5463 1084.6171 1084.6142 2.69 1 10 0.79 +1Score > 39 indicates identity
Score > 21 indicates homology
QGAKGLAWVR
1542 610.8353 1219.6560 1219.6482 6.44 1 10 0.74 +1Score > 40 indicates identity
Score > 21 indicates homology
AELSTQLAMLK + Oxidation (M)
1078 501.2968 1000.5790 1000.5627 16.3 0 10 0.88 +1Score > 39 indicates identity
Score > 21 indicates homology
VIPMIQGVK + Deamidated (NQ); Oxidation (M)
3422 547.7708 2187.0541 2187.0956 -19.0 1 10 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
DGSSKILFIYICPMQATVK + Deamidated (NQ); Oxidation (M)
1921 467.5785 1399.7137 1399.7195 -4.13 0 10 0.68 +1Score > 40 indicates identity
Score > 20 indicates homology
QAVTNPNVVQSIK + 3 Deamidated (NQ)
3092 1007.0284 2012.0422 2012.0513 -4.51 1 10 0.42 +1Score > 39 indicates identity
Score > 18 indicates homology
TRMYDIVNVINVIYQR + Oxidation (M)
3657 578.8170 2311.2389 2311.2205 7.94 1 10 0.41 +1Score > 36 indicates identity
Score > 18 indicates homology
KQSVPANILSLQLQQMAANLE + Oxidation (M)
2273 523.6260 1567.8562 1567.8570 -0.51 0 10 0.5 +1Score > 38 indicates identity
Score > 19 indicates homology
AIIQPGGSINDGVSIK
2416 812.4299 1622.8452 1622.8416 2.23 0 10 0.75 +1Score > 40 indicates identity
Score > 21 indicates homology
HGVQLNAPAAFQLVR + 3 Deamidated (NQ)
3890 835.4440 2503.3102 2503.3434 -13.3 0 10 0.59 +1Score > 36 indicates identity
Score > 20 indicates homology
LVQQPQNASTFINRPALGILPPE + Deamidated (NQ)
2824 922.9544 1843.8942 1843.8873 3.76 1 9 0.61 +1Score > 40 indicates identity
Score > 20 indicates homology
GAQQTLPMGPVASQEAIK + 3 Deamidated (NQ); Oxidation (M)
3055 994.5321 1987.0496 1987.0700 -10.2 1 9 0.88 +1Score > 38 indicates identity
Score > 21 indicates homology
INAAWGMTVLLLDVLTEK + Deamidated (NQ)
3927 635.8577 2539.4017 2539.3873 5.69 1 9 1 +1Score > 33 indicates identity
Score > 22 indicates homology
NWTKLGVALFGLGQVLVLSSMYK + Oxidation (M)
1670 426.5831 1276.7275 1276.7173 7.98 1 9 0.9 +1Score > 36 indicates identity
Score > 21 indicates homology
SNLVTLSKIMR + Oxidation (M)
2912 631.6852 1892.0338 1892.0367 -1.56 0 9 0.59 +1Score > 36 indicates identity
Score > 20 indicates homology
RPSSLVHSIVGQVITGIE + Deamidated (NQ)
2272 784.9191 1567.8236 1567.8093 9.14 0 9 0.85 +1Score > 39 indicates identity
Score > 21 indicates homology
AAGDISQLNLGLVAPE + Deamidated (NQ)
2929 636.7192 1907.1358 1907.0979 19.9 1 9 1 +1Score > 27 indicates identity
Score > 22 indicates homology
LPVLGAVIKNNILDVVTE + Deamidated (NQ)
3186 692.7156 2075.1250 2075.1296 -2.25 1 9 0.25 +1Score > 36 indicates identity
Score > 16 indicates homology
SVVINTLCQAQTKLGLTTK + Deamidated (NQ)
2139 502.5969 1504.7689 1504.7643 3.07 1 9 0.54 +1Score > 41 indicates identity
Score > 19 indicates homology
WALQAMGKMAGGIR + Oxidation (M)
2510 557.3364 1668.9874 1668.9563 18.6 1 9 0.28 +1Score > 30 indicates identity
Score > 16 indicates homology
SRLPLLDLNIYVPR + Deamidated (NQ)
2708 592.3000 1773.8782 1773.8686 5.40 1 9 0.39 +1Score > 40 indicates identity
Score > 18 indicates homology
LTQHFAGQLAENGFIE
3012 983.5090 1965.0034 1964.9990 2.28 1 9 0.24 +1Score > 39 indicates identity
Score > 16 indicates homology
AGARAGMVAVDQTTIDYVK
2988 971.5430 1941.0714 1941.0935 -11.4 0 9 0.6 +1Score > 35 indicates identity
Score > 20 indicates homology
LLIVDRPIDQIAPVIHE + Deamidated (NQ)
3272 1060.5692 2119.1238 2119.1161 3.65 1 9 0.6 +1Score > 37 indicates identity
Score > 20 indicates homology
ILFLNGRDLQSVGVTSTGLE + Deamidated (NQ)
2357 802.9640 1603.9134 1603.8855 17.5 1 9 0.91 +1Score > 34 indicates identity
Score > 21 indicates homology
DAAILLKSMASLDLK + Oxidation (M)
2554 565.2874 1692.8404 1692.8280 7.30 1 9 0.79 +1Score > 41 indicates identity
Score > 21 indicates homology
DALAVQEAGAFMIVIE + Deamidated (NQ); Oxidation (M)
2706 591.9731 1772.8975 1772.8958 0.95 1 9 0.66 +1Score > 40 indicates identity
Score > 20 indicates homology
RPNPDFVLNQPRYR + 2 Deamidated (NQ)
3399 727.0280 2178.0622 2178.0779 -7.21 1 9 1 +1Score > 40 indicates identity
Score > 22 indicates homology
RPLNMDFVLNQPKYQNAK + 3 Deamidated (NQ)
1941 705.3650 1408.7154 1408.7198 -3.08 1 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
VAQLTAIQLHGEE + Deamidated (NQ)
2352 534.6314 1600.8724 1600.8671 3.27 1 9 0.82 +1Score > 38 indicates identity
Score > 21 indicates homology
LSLIKNVNINQTNK + 3 Deamidated (NQ)
3329 1072.5737 2143.1328 2143.1455 -5.89 1 9 0.4 +1Score > 37 indicates identity
Score > 18 indicates homology
LLLPGTRLMMLPTDMALIE + Oxidation (M)
3029 987.0555 1972.0964 1972.1132 -8.49 1 9 0.44 +1Score > 35 indicates identity
Score > 18 indicates homology
IILVILDAISNIFQAAEK + 2 Deamidated (NQ)
2823 615.3547 1843.0423 1843.0567 -7.84 1 9 0.8 +1Score > 34 indicates identity
Score > 21 indicates homology
ISDQLVILAGKNGFVLR + Deamidated (NQ)
1509 604.3262 1206.6378 1206.6244 11.1 1 9 0.83 +1Score > 41 indicates identity
Score > 21 indicates homology
YIASGDIIRAE
3605 761.4015 2281.1827 2281.1372 19.9 1 9 0.41 +1Score > 38 indicates identity
Score > 18 indicates homology
MTGALNNSGTTAIFINQLRDK + Deamidated (NQ); Oxidation (M)
314 335.7116 669.4086 669.4173 -13.0 1 9 0.81 +1Score > 34 indicates identity
Score > 21 indicates homology
GAPKAVK
918 469.7344 937.4542 937.4505 4.01 1 9 0.93 +1Score > 41 indicates identity
Score > 21 indicates homology
SGAYENLGK
1658 425.5523 1273.6351 1273.6455 -8.19 1 9 0.68 +1Score > 41 indicates identity
Score > 20 indicates homology
GEFPALAWAQGK
2830 617.6556 1849.9450 1849.9145 16.5 0 9 0.64 +1Score > 40 indicates identity
Score > 20 indicates homology
GHVLFAVGAMADYGALSR + Oxidation (M)
3386 544.0209 2172.0545 2172.0348 9.05 1 9 0.73 +1Score > 39 indicates identity
Score > 20 indicates homology
EGQDQFPIFHVNNQSSLGR
1265 548.3342 1094.6538 1094.6560 -1.96 1 9 0.9 +1Score > 33 indicates identity
Score > 21 indicates homology
ARVALPQALR + Deamidated (NQ)
3318 712.7375 2135.1907 2135.1813 4.41 0 9 0.64 +1Score > 33 indicates identity
Score > 20 indicates homology
VLTIVAMLNVQNIFYRPK + Deamidated (NQ); Oxidation (M)
2364 804.8959 1607.7772 1607.7977 -12.7 1 9 0.46 +1Score > 41 indicates identity
Score > 18 indicates homology
LQSVDGYMNIALER
2782 605.6306 1813.8700 1813.8339 19.9 1 9 0.88 +1Score > 40 indicates identity
Score > 21 indicates homology
LCSLTLDYCNTAVER
963 477.7557 953.4968 953.5042 -7.75 1 9 0.95 +1Score > 39 indicates identity
Score > 22 indicates homology
HLARNSQK + Deamidated (NQ)
1168 521.8015 1041.5884 1041.5818 6.34 0 9 0.81 +1Score > 40 indicates identity
Score > 21 indicates homology
TINPDTLIR
2913 947.4974 1892.9802 1892.9731 3.75 1 9 0.37 +1Score > 39 indicates identity
Score > 17 indicates homology
GGVVVASKDGSIVVDYSIE
264 318.2106 634.4066 634.4054 2.00 0 9 0.18 +1Score > 26 indicates identity
Score > 14 indicates homology
IYIVK
3147 512.0320 2044.0989 2044.0629 17.6 1 9 0.78 +1Score > 37 indicates identity
Score > 21 indicates homology
FINAALNQELFVSIAQHK + 2 Deamidated (NQ)
3778 800.0892 2397.2458 2397.2838 -15.9 1 9 0.23 +1Score > 37 indicates identity
Score > 15 indicates homology
TRGGLTTNAVYGFTAMLINLLR + Oxidation (M)
563 392.7516 783.4886 783.4967 -10.2 0 9 0.88 +1Score > 35 indicates identity
Score > 21 indicates homology
VLGLNIR
2534 837.9768 1673.9390 1673.9135 15.3 1 9 0.77 +1Score > 35 indicates identity
Score > 21 indicates homology
IASAITKVTGGVGPMTR + Oxidation (M)
2758 902.4748 1802.9350 1802.9488 -7.64 0 9 0.85 +1Score > 39 indicates identity
Score > 21 indicates homology
TFTQLLQMGVIPIVNE + Deamidated (NQ)
4351 998.4937 2992.4593 2992.5150 -18.6 0 9 0.22 +1Score > 37 indicates identity
Score > 15 indicates homology
ALCAQTMLVSHPGVNVLLLDDGLQHYK + Deamidated (NQ)
415 357.2140 712.4134 712.4119 2.14 1 9 0.89 +1Score > 38 indicates identity
Score > 21 indicates homology
EAIVPGK
2483 555.2981 1662.8725 1662.8940 -13.0 1 9 0.48 +1Score > 40 indicates identity
Score > 19 indicates homology
LAQKYNTISQLLDR + Deamidated (NQ)
3468 735.0875 2202.2407 2202.2293 5.16 1 9 0.4 +1Score > 32 indicates identity
Score > 18 indicates homology
TAIVMILTNTNTAQAKSLLAK + Deamidated (NQ)
2575 427.4866 1705.9173 1705.9111 3.65 1 9 0.67 +1Score > 38 indicates identity
Score > 20 indicates homology
NAKLNNLNNVNIVHK + 2 Deamidated (NQ)
583 395.7099 789.4052 789.4167 -14.5 1 9 0.72 +1Score > 42 indicates identity
Score > 20 indicates homology
KADVMAR
2526 837.4676 1672.9206 1672.9069 8.20 1 9 0.26 +1Score > 37 indicates identity
Score > 16 indicates homology
KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
2655 583.6740 1748.0002 1747.9832 9.71 1 9 0.77 +1Score > 33 indicates identity
Score > 21 indicates homology
LVASAYSIAQKAGTIVR + Deamidated (NQ)
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