MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3136 680.3479 2038.0219 2038.0485 -13.1 1 11 0.38 +1Score > 39 indicates identity
Score > 19 indicates homology
VNLFTLLSLWMELFPAE + Oxidation (M)
3135 680.3476 2038.0210 2038.0485 -13.5 1 11 0.23 +1Score > 39 indicates identity
Score > 17 indicates homology
VNLFTLLSLWMELFPAE + Oxidation (M)
598 399.2317 796.4488 796.4443 5.70 0 11 0.65 +1Score > 36 indicates identity
Score > 21 indicates homology
GAPAVTPGK
1850 683.3278 1364.6410 1364.6242 12.4 1 11 0.68 +1Score > 41 indicates identity
Score > 21 indicates homology
VSAQLDRVMSDE + Oxidation (M)
2518 835.8810 1669.7474 1669.7440 2.08 1 11 0.44 +1Score > 39 indicates identity
Score > 20 indicates homology
ALQFQMMQDELQR + Deamidated (NQ); 2 Oxidation (M)
4148 925.8413 2774.5021 2774.4855 5.99 1 11 0.71 +1Score > 33 indicates identity
Score > 22 indicates homology
VQLAYAIGVAQPVSVRVDTFGTALVAE + Deamidated (NQ)
1866 458.2684 1371.7834 1371.7874 -2.96 1 11 0.57 +1Score > 36 indicates identity
Score > 21 indicates homology
AIKVLSVDWSVR
2990 648.3644 1942.0714 1942.0497 11.2 1 11 0.48 +1Score > 35 indicates identity
Score > 20 indicates homology
GRQAIANSLRPNDIVYR
1931 351.9376 1403.7213 1403.7310 -6.88 1 11 0.76 +1Score > 41 indicates identity
Score > 22 indicates homology
AGPRPEGYFAALR
1971 712.3604 1422.7062 1422.7242 -12.6 1 11 0.71 +1Score > 41 indicates identity
Score > 22 indicates homology
ILGQSLSNAYVEK + 2 Deamidated (NQ)
2221 516.2770 1545.8092 1545.8185 -6.02 0 11 0.87 +1Score > 40 indicates identity
Score > 22 indicates homology
KPMQGIITAVGTGTR + Deamidated (NQ); Oxidation (M)
3546 749.7443 2246.2111 2246.1776 14.9 1 11 1 +1Score > 35 indicates identity
Score > 23 indicates homology
AVTHAIVGMLPHNSLGRQMAK + Oxidation (M)
3317 712.7121 2135.1145 2135.0833 14.6 1 10 0.21 +1Score > 38 indicates identity
Score > 16 indicates homology
LVHHDIIDALENMSRPFK + Deamidated (NQ)
3795 806.4443 2416.3111 2416.3287 -7.30 0 10 0.22 +1Score > 34 indicates identity
Score > 16 indicates homology
ISLLNGVSLIVGNMIGSGIFVSPK + 2 Deamidated (NQ)
822 450.2711 898.5276 898.5348 -7.97 1 10 0.76 +1Score > 39 indicates identity
Score > 22 indicates homology
LKAIANNR
1063 498.7816 995.5486 995.5400 8.71 0 10 0.76 +1Score > 37 indicates identity
Score > 22 indicates homology
VSDAPPALAR
1530 405.8964 1214.6674 1214.6619 4.52 1 10 0.62 +1Score > 40 indicates identity
Score > 21 indicates homology
LTAASGIEQAVR
1824 451.9030 1352.6872 1352.7122 -18.5 0 10 0.63 +1Score > 40 indicates identity
Score > 21 indicates homology
HINPMATALIQK + Deamidated (NQ); Oxidation (M)
3219 699.0425 2094.1057 2094.0892 7.89 1 10 0.27 +1Score > 37 indicates identity
Score > 17 indicates homology
RAIMLNQPNPQDGVDVLAK + Oxidation (M)
2540 842.4706 1682.9266 1682.9277 -0.61 0 10 0.91 +1Score > 37 indicates identity
Score > 23 indicates homology
QIVPASSVNVIMIAPK + Deamidated (NQ); Oxidation (M)
2718 594.6386 1780.8940 1780.9182 -13.6 1 10 0.25 +1Score > 40 indicates identity
Score > 17 indicates homology
QKPGLYDMRDILGFK + Deamidated (NQ)
3675 1162.6356 2323.2566 2323.2886 -13.7 1 10 0.15 +1Score > 35 indicates identity
Score > 15 indicates homology
TLINLNKQIVITSDKPPGQLK + 4 Deamidated (NQ)
3747 594.0958 2372.3541 2372.3176 15.4 1 10 0.34 +1Score > 30 indicates identity
Score > 18 indicates homology
IQVSNFRTGISNILVVTDVAAR
2047 366.4665 1461.8369 1461.8113 17.5 1 10 0.49 +1Score > 35 indicates identity
Score > 20 indicates homology
MTVLTQEAIITVK + Oxidation (M)
3461 733.3937 2197.1593 2197.1954 -16.4 1 10 0.26 +1Score > 37 indicates identity
Score > 17 indicates homology
AQGELLLGSITALQQGLVASAR + 2 Deamidated (NQ)
362 689.3807 688.3734 688.3616 17.2 1 10 0.46 +1Score > 44 indicates identity
Score > 20 indicates homology
RAANTR + Deamidated (NQ)
2366 805.3829 1608.7512 1608.7600 -5.42 1 10 0.82 +1Score > 40 indicates identity
Score > 22 indicates homology
KMGMNIINDDITGR + 2 Oxidation (M)
3158 685.0470 2052.1192 2052.0990 9.82 1 10 0.34 +1Score > 35 indicates identity
Score > 18 indicates homology
IAESINIAVDSVPNVVAVIE + Deamidated (NQ)
3167 687.0248 2058.0526 2058.0343 8.86 1 10 1 +1Score > 39 indicates identity
Score > 23 indicates homology
MLPPLVTGAPLSFLESQNE + Oxidation (M)
1876 692.8239 1383.6332 1383.6228 7.56 0 10 0.98 +1Score > 40 indicates identity
Score > 23 indicates homology
IDDAQDMTAFLK + Deamidated (NQ); Oxidation (M)
2462 823.9182 1645.8218 1645.7995 13.6 0 10 0.7 +1Score > 41 indicates identity
Score > 21 indicates homology
EPRPGLAQDPPGCPR
3052 994.4756 1986.9366 1986.9494 -6.41 1 10 0.6 +1Score > 39 indicates identity
Score > 21 indicates homology
DASGNTAGAALKPLSNDEVR + 2 Deamidated (NQ)
3903 837.4699 2509.3879 2509.3462 16.6 1 10 0.66 +1Score > 32 indicates identity
Score > 21 indicates homology
VAEMLAPVLTGNDLILVQGAGNIGK + Deamidated (NQ); Oxidation (M)
403 352.2222 702.4298 702.4276 3.23 0 10 0.83 +1Score > 38 indicates identity
Score > 22 indicates homology
ITQTIK
1647 633.8353 1265.6560 1265.6768 -16.4 1 10 0.89 +1Score > 40 indicates identity
Score > 22 indicates homology
VDYVRVYQPK
1705 648.8273 1295.6400 1295.6622 -17.1 1 10 0.94 +1Score > 41 indicates identity
Score > 23 indicates homology
IRTYNFPQTR + Deamidated (NQ)
3201 696.0272 2085.0598 2085.0432 7.93 0 10 0.58 +1Score > 39 indicates identity
Score > 20 indicates homology
TTSGANFVFHWPLQPQVR + Deamidated (NQ)
2723 893.4860 1784.9574 1784.9342 13.0 1 10 0.35 +1Score > 38 indicates identity
Score > 18 indicates homology
NMINIKQILNNVQIE + 2 Deamidated (NQ)
2750 601.3329 1800.9769 1800.9886 -6.54 1 10 0.77 +1Score > 37 indicates identity
Score > 22 indicates homology
IHSNFINDLLGGKFVK
2659 877.4649 1752.9152 1752.9482 -18.8 1 10 0.61 +1Score > 39 indicates identity
Score > 21 indicates homology
IDTALRGASDILAPQGR
3850 822.4515 2464.3327 2464.3550 -9.06 1 10 0.19 +1Score > 34 indicates identity
Score > 16 indicates homology
QLRPYVIDASLLIDQAVRTHR + Deamidated (NQ)
2850 620.9968 1859.9686 1859.9736 -2.73 1 10 0.38 +1Score > 39 indicates identity
Score > 19 indicates homology
IVLQPDETIQISMMVK + Oxidation (M)
3668 773.7609 2318.2609 2318.2589 0.84 1 10 1 +1Score > 34 indicates identity
Score > 23 indicates homology
IASTISMMAILVIGTVLVQRGE + Deamidated (NQ); Oxidation (M)
2604 860.4598 1718.9050 1718.8938 6.53 1 10 0.91 +1Score > 39 indicates identity
Score > 22 indicates homology
GTEVIIITTDTSPSGTK
3989 869.1158 2604.3256 2604.3188 2.61 1 10 0.39 +1Score > 37 indicates identity
Score > 19 indicates homology
KGMLGMIVYLGMMAGAFILGGLADK + 3 Oxidation (M)
1464 593.3260 1184.6374 1184.6553 -15.1 1 10 0.97 +1Score > 40 indicates identity
Score > 23 indicates homology
KSIAFSLTYR
1842 454.2545 1359.7417 1359.7259 11.6 0 10 0.9 +1Score > 39 indicates identity
Score > 22 indicates homology
VLDNHTALAHIR + Deamidated (NQ)
4028 663.0621 2648.2193 2648.2581 -14.6 1 10 0.2 +1Score > 37 indicates identity
Score > 16 indicates homology
MPPSPLSSGAFYQRWLGPVLANDE + Deamidated (NQ); Oxidation (M)
3134 1020.0175 2038.0204 2038.0081 6.06 0 10 0.35 +1Score > 39 indicates identity
Score > 18 indicates homology
VTYLEPMANGLLDLDAFR + Deamidated (NQ)
3179 688.0425 2061.1057 2061.0717 16.5 1 10 0.44 +1Score > 37 indicates identity
Score > 19 indicates homology
RPEVMGGLGGFGALYALPQK + Deamidated (NQ)
2240 778.3484 1554.6822 1554.7103 -18.0 0 10 0.13 +1Score > 38 indicates identity
Score > 14 indicates homology
SNWEPNFGLTFSR + Deamidated (NQ)
2385 538.6068 1612.7986 1612.8236 -15.5 1 10 0.39 +1Score > 40 indicates identity
Score > 19 indicates homology
TIYIIDFKTQLQE + 2 Deamidated (NQ)
2933 956.4886 1910.9626 1910.9486 7.35 0 10 0.57 +1Score > 39 indicates identity
Score > 20 indicates homology
YVIQTQINPQLANHGGR + 3 Deamidated (NQ)
1052 496.7816 991.5486 991.5338 14.9 1 10 0.68 +1Score > 39 indicates identity
Score > 21 indicates homology
TVYKKPQE
1453 394.5478 1180.6216 1180.6200 1.31 1 10 0.76 +1Score > 39 indicates identity
Score > 22 indicates homology
KQTHNVVNIE
2570 568.3350 1701.9832 1701.9890 -3.40 1 10 0.42 +1Score > 32 indicates identity
Score > 19 indicates homology
IVYQNLTRINISIR
1614 415.5681 1243.6825 1243.7023 -15.9 1 10 0.83 +1Score > 40 indicates identity
Score > 22 indicates homology
IAIITNNNKLK + 3 Deamidated (NQ)
2529 558.6525 1672.9357 1672.9359 -0.15 1 10 0.48 +1Score > 36 indicates identity
Score > 20 indicates homology
LVQSQLLKSVVSGSAR + 2 Deamidated (NQ)
3088 671.3335 2010.9787 2010.9608 8.88 1 10 0.69 +1Score > 40 indicates identity
Score > 21 indicates homology
AYEADPTSIFGGIIALNME
1934 703.3398 1404.6650 1404.6779 -9.16 1 10 0.34 +1Score > 41 indicates identity
Score > 18 indicates homology
AAARMADDLASAAR + Oxidation (M)
2220 516.2686 1545.7840 1545.7708 8.50 0 10 0.33 +1Score > 41 indicates identity
Score > 18 indicates homology
IDTMIPLNLTNQR + 2 Deamidated (NQ); Oxidation (M)
2600 859.4639 1716.9132 1716.9410 -16.2 0 10 0.96 +1Score > 39 indicates identity
Score > 23 indicates homology
ALISLVFNNLQTINR + 2 Deamidated (NQ)
2983 646.0320 1935.0742 1935.0717 1.28 1 10 0.14 +1Score > 34 indicates identity
Score > 14 indicates homology
RILIFSQFTQVLDILE + Deamidated (NQ)
3344 717.0565 2148.1477 2148.1356 5.62 0 10 0.44 +1Score > 36 indicates identity
Score > 19 indicates homology
NMGTLALMGLIMTLLAQASAK + Deamidated (NQ)
3665 772.7204 2315.1394 2315.1065 14.2 1 10 0.25 +1Score > 39 indicates identity
Score > 17 indicates homology
MNNYETVFILTPVLSDAQMK + 2 Deamidated (NQ)
1738 657.8776 1313.7406 1313.7191 16.4 1 10 0.79 +1Score > 38 indicates identity
Score > 22 indicates homology
TKDQIVSINPAK + Deamidated (NQ)
2825 615.9751 1844.9035 1844.9156 -6.59 1 10 0.95 +1Score > 40 indicates identity
Score > 22 indicates homology
GTVGIGVPGFVNQETGIAE + Deamidated (NQ)
2638 867.4788 1732.9430 1732.9260 9.82 1 10 0.51 +1Score > 37 indicates identity
Score > 20 indicates homology
HYSIARQVQYVLQK + Deamidated (NQ)
2925 636.0095 1905.0067 1904.9731 17.6 1 10 0.71 +1Score > 38 indicates identity
Score > 21 indicates homology
LLTELVADFSDQLANVR + 2 Deamidated (NQ)
1671 639.3723 1276.7300 1276.7292 0.67 1 10 0.33 +1Score > 35 indicates identity
Score > 18 indicates homology
KALFAPFQVTR
2677 588.6656 1762.9750 1762.9941 -10.9 0 10 0.51 +1Score > 36 indicates identity
Score > 20 indicates homology
VLGLGLPGNGTTLAIDPR
3253 1057.5870 2113.1594 2113.1241 16.7 1 10 0.96 +1Score > 35 indicates identity
Score > 22 indicates homology
ILFAGTPSCAVPALNLIARE + Deamidated (NQ)
2325 529.9675 1586.8807 1586.8529 17.5 0 10 0.51 +1Score > 37 indicates identity
Score > 20 indicates homology
NPVIPSPTPAANRPR + Deamidated (NQ)
1022 488.7795 975.5444 975.5389 5.66 1 10 0.38 +1Score > 40 indicates identity
Score > 18 indicates homology
AVEGLFNVK
2752 901.4979 1800.9812 1800.9768 2.48 1 10 0.36 +1Score > 37 indicates identity
Score > 18 indicates homology
VIVGLPRMLDGSAGSSVK + Oxidation (M)
3419 729.4041 2185.1905 2185.1491 18.9 0 10 0.37 +1Score > 35 indicates identity
Score > 18 indicates homology
DTAVASHVYAAVISLTQNAVR
3175 687.3594 2059.0564 2059.0837 -13.3 1 10 0.99 +1Score > 39 indicates identity
Score > 23 indicates homology
ALLNHPDIEVLGVVTQPDK + 2 Deamidated (NQ)
2391 538.6510 1612.9312 1612.9413 -6.28 1 10 0.41 +1Score > 33 indicates identity
Score > 19 indicates homology
TLNLLRTLGFPVNR
1425 585.8290 1169.6434 1169.6404 2.61 0 10 0.85 +1Score > 40 indicates identity
Score > 22 indicates homology
QALINNGIAVR + 2 Deamidated (NQ)
2097 746.4152 1490.8158 1490.8027 8.81 1 10 0.39 +1Score > 38 indicates identity
Score > 18 indicates homology
ALLLNLQHMHRK + 2 Deamidated (NQ); Oxidation (M)
1734 437.2237 1308.6493 1308.6449 3.35 1 10 0.82 +1Score > 42 indicates identity
Score > 22 indicates homology
DLQTEINSFLK + 2 Deamidated (NQ)
2187 510.6252 1528.8538 1528.8402 8.88 0 10 0.57 +1Score > 37 indicates identity
Score > 20 indicates homology
AFNIDLGFPLIGPR
2224 774.8895 1547.7644 1547.7831 -12.1 1 10 0.71 +1Score > 41 indicates identity
Score > 21 indicates homology
NVVEVTFNDLAAQK + Deamidated (NQ)
2922 635.0297 1902.0673 1902.0364 16.3 0 10 0.28 +1Score > 34 indicates identity
Score > 17 indicates homology
FGQVVGTGNLADFIPVLR
1408 387.8890 1160.6452 1160.6475 -1.99 1 10 0.79 +1Score > 40 indicates identity
Score > 21 indicates homology
AGIMLLGTEIK + Oxidation (M)
1527 607.8582 1213.7018 1213.7142 -10.2 1 10 0.77 +1Score > 38 indicates identity
Score > 21 indicates homology
LIGISGRIQTR + Deamidated (NQ)
1849 682.3802 1362.7458 1362.7554 -7.01 1 10 0.6 +1Score > 38 indicates identity
Score > 20 indicates homology
VLIRNAGHMVPR + Deamidated (NQ)
2881 940.4811 1878.9476 1878.9112 19.4 0 10 0.79 +1Score > 40 indicates identity
Score > 21 indicates homology
SDALGWQYITGTLPDSR
3071 667.0062 1997.9968 1997.9953 0.73 0 10 0.88 +1Score > 40 indicates identity
Score > 22 indicates homology
IGDHTVIAGCAGVAGSTTVGR
1781 665.3529 1328.6912 1328.6823 6.70 1 10 0.76 +1Score > 41 indicates identity
Score > 21 indicates homology
LDVADEADILQK
1241 362.5463 1084.6171 1084.6142 2.69 1 10 0.75 +1Score > 39 indicates identity
Score > 21 indicates homology
QGAKGLAWVR
1573 411.2423 1230.7051 1230.7006 3.66 1 10 0.66 +1Score > 37 indicates identity
Score > 21 indicates homology
KNQLILDLMK + Oxidation (M)
3198 1042.0416 2082.0686 2082.0932 -11.8 1 10 0.23 +1Score > 38 indicates identity
Score > 16 indicates homology
AFASLGPALRHQMLDLVNK + 2 Deamidated (NQ)
2232 776.3936 1550.7726 1550.7987 -16.8 1 10 0.87 +1Score > 41 indicates identity
Score > 22 indicates homology
QRLMTAQNLASYR
3306 1068.0367 2134.0588 2134.0291 14.0 1 10 0.5 +1Score > 39 indicates identity
Score > 19 indicates homology
TGEGSAVFNGAQNGVSQLIQR + 2 Deamidated (NQ)
1699 647.3215 1292.6284 1292.6394 -8.51 1 10 0.8 +1Score > 41 indicates identity
Score > 21 indicates homology
NCAGKTVTVTNK + Deamidated (NQ)
2550 844.4824 1686.9502 1686.9417 5.07 0 10 0.34 +1Score > 35 indicates identity
Score > 18 indicates homology
VPKPDQDLVLLSAHR
4300 974.4777 2920.4113 2920.4429 -10.8 1 10 0.19 +1Score > 37 indicates identity
Score > 15 indicates homology
LCLRPSSSVFLGDQQLYFTQEYLR + Deamidated (NQ)
209 302.6734 603.3322 603.3228 15.7 0 10 0.93 +1Score > 44 indicates identity
Score > 22 indicates homology
ATDLGK
787 443.7392 885.4638 885.4668 -3.35 1 10 0.41 +1Score > 40 indicates identity
Score > 19 indicates homology
EAQNGLVR
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