MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3818)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1598 620.3252 1238.6358 1238.6394 -2.88 0 11 0.69 +1Score > 40 indicates identity
Score > 22 indicates homology
VNGAPITLVQPE + 2 Deamidated (NQ)
271 322.1884 642.3622 642.3561 9.51 1 11 0.42 +1Score > 35 indicates identity
Score > 20 indicates homology
AGRSPR
1256 544.7917 1087.5688 1087.5621 6.16 1 11 0.66 +1Score > 42 indicates identity
Score > 22 indicates homology
KATAAGQAQNK + Deamidated (NQ)
2021 482.9273 1445.7601 1445.7626 -1.77 1 11 0.78 +1Score > 40 indicates identity
Score > 23 indicates homology
AVYNRLSQLPQR + 2 Deamidated (NQ)
2350 534.2999 1599.8779 1599.8719 3.70 1 11 0.52 +1Score > 38 indicates identity
Score > 21 indicates homology
QAGEIVTTVLNGGVLK + 2 Deamidated (NQ)
437 361.2172 720.4198 720.4204 -0.74 1 11 0.76 +1Score > 38 indicates identity
Score > 23 indicates homology
MSKIVK + Oxidation (M)
3885 834.7808 2501.3206 2501.2975 9.24 0 11 0.25 +1Score > 36 indicates identity
Score > 18 indicates homology
IILGPGSLYTSIIPNLLVNDVCE + 2 Deamidated (NQ)
3458 733.3654 2197.0744 2197.0474 12.3 1 11 0.97 +1Score > 39 indicates identity
Score > 24 indicates homology
VDTEGVPFTSNVAANINYMR
3100 674.0098 2019.0076 2019.0421 -17.1 0 11 0.12 +1Score > 40 indicates identity
Score > 15 indicates homology
TVQITIMSLIAMTVYFR + Deamidated (NQ); 2 Oxidation (M)
3974 862.4521 2584.3345 2584.3393 -1.87 1 11 0.41 +1Score > 37 indicates identity
Score > 20 indicates homology
VDPVNFKLLSQCIQVVLAAHMGK + 2 Deamidated (NQ); Oxidation (M)
2390 538.6508 1612.9306 1612.9148 9.79 1 11 0.8 +1Score > 33 indicates identity
Score > 23 indicates homology
STLVNALLAQDKAIR + Deamidated (NQ)
2409 809.4390 1616.8634 1616.8774 -8.61 1 11 0.25 +1Score > 39 indicates identity
Score > 18 indicates homology
DTIKYLLSVDSVHK
2693 590.0095 1767.0067 1767.0003 3.63 1 11 0.7 +1Score > 33 indicates identity
Score > 22 indicates homology
TLQVVRLSQNATIPAR + Deamidated (NQ)
760 437.7159 873.4172 873.4344 -19.7 1 11 0.47 +1Score > 42 indicates identity
Score > 20 indicates homology
LKHNFSE
3051 663.3173 1986.9301 1986.9534 -11.7 1 11 0.41 +1Score > 39 indicates identity
Score > 20 indicates homology
FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
3554 751.3671 2251.0795 2251.0467 14.6 1 11 1 +1Score > 39 indicates identity
Score > 24 indicates homology
YAVLSDILGNEDHLGDMDFK
2199 512.6509 1534.9309 1534.9123 12.1 0 11 0.59 +1Score > 28 indicates identity
Score > 21 indicates homology
VTLLVNYFLLIAR + Deamidated (NQ)
3928 509.6468 2543.1976 2543.2254 -10.9 1 11 0.22 +1Score > 38 indicates identity
Score > 17 indicates homology
LIKSDFQNCGQLNVWYITTIE + 2 Deamidated (NQ)
2116 749.9398 1497.8650 1497.8402 16.6 1 11 0.97 +1Score > 34 indicates identity
Score > 24 indicates homology
ISIAGRLVADLLQE + Deamidated (NQ)
2960 962.4608 1922.9070 1922.8900 8.86 0 11 0.33 +1Score > 40 indicates identity
Score > 19 indicates homology
QVAMAGDGQVTMGQATVMK + Deamidated (NQ)
885 308.5163 922.5271 922.5236 3.76 1 11 0.87 +1Score > 37 indicates identity
Score > 23 indicates homology
IPNPVVER
665 417.2211 832.4276 832.4331 -6.50 0 11 0.8 +1Score > 43 indicates identity
Score > 23 indicates homology
VDPNIFK + Deamidated (NQ)
250 315.2036 628.3926 628.3908 2.95 0 11 0.94 +1Score > 42 indicates identity
Score > 23 indicates homology
AQIVAK
904 464.7452 927.4758 927.4603 16.8 0 11 0.8 +1Score > 40 indicates identity
Score > 23 indicates homology
YAYGWLR
1667 320.1834 1276.7045 1276.7247 -15.8 0 11 0.48 +1Score > 38 indicates identity
Score > 21 indicates homology
IIMILSMALTR + Oxidation (M)
2759 601.9860 1802.9362 1802.9414 -2.90 0 11 0.53 +1Score > 39 indicates identity
Score > 21 indicates homology
VSAVIILQSYLDNPDR + Deamidated (NQ)
2052 733.4304 1464.8462 1464.8552 -6.11 1 11 0.71 +1Score > 34 indicates identity
Score > 22 indicates homology
VVLIDVVEGIPQGK
2557 848.9313 1695.8480 1695.8567 -5.12 1 11 0.27 +1Score > 40 indicates identity
Score > 18 indicates homology
VSVPVGDVVVVPTEGNE + Deamidated (NQ)
3151 682.6954 2045.0644 2045.0629 0.74 1 11 0.45 +1Score > 38 indicates identity
Score > 20 indicates homology
MAKAHDLIAWNAPHSLVR + Oxidation (M)
3426 730.3746 2188.1020 2188.1173 -6.99 1 11 1 +1Score > 39 indicates identity
Score > 24 indicates homology
ICNGRYFINTAMILFLNK + Deamidated (NQ)
3497 741.4033 2221.1881 2221.1531 15.7 1 11 0.81 +1Score > 36 indicates identity
Score > 23 indicates homology
YNIYVQAINFPTVPIGQER
3692 1169.6019 2337.1892 2337.1746 6.25 1 11 0.99 +1Score > 38 indicates identity
Score > 24 indicates homology
RNVSAALQAYAMLTTSAANGAVR + 2 Deamidated (NQ)
1111 339.2160 1014.6262 1014.6226 3.55 1 11 0.61 +1Score > 34 indicates identity
Score > 21 indicates homology
FLELKPLR
1116 508.7743 1015.5340 1015.5186 15.2 0 11 0.88 +1Score > 42 indicates identity
Score > 23 indicates homology
QLVQNGSLVG + 2 Deamidated (NQ)
3162 686.3687 2056.0843 2056.0550 14.2 0 11 0.18 +1Score > 38 indicates identity
Score > 16 indicates homology
YLQDQLIIFMALASGISR + 2 Deamidated (NQ); Oxidation (M)
3752 1190.6453 2379.2760 2379.2355 17.0 1 11 0.98 +1Score > 35 indicates identity
Score > 24 indicates homology
TNLGNFLSKINMNTIDIQITK + 2 Deamidated (NQ)
999 323.8390 968.4952 968.4815 14.2 1 11 0.71 +1Score > 39 indicates identity
Score > 22 indicates homology
HIEILDTE
664 416.7520 831.4894 831.4966 -8.65 0 11 0.46 +1Score > 38 indicates identity
Score > 20 indicates homology
AILFAAAR
3664 772.7196 2315.1370 2315.1393 -1.01 1 11 0.31 +1Score > 39 indicates identity
Score > 18 indicates homology
ALDIRPDNFVLIATNNQDRE + 2 Deamidated (NQ)
1058 498.2637 994.5128 994.4940 19.0 0 11 0.42 +1Score > 41 indicates identity
Score > 20 indicates homology
SLTAVAMMR + Oxidation (M)
2964 963.0161 1924.0176 1923.9836 17.7 1 11 0.7 +1Score > 38 indicates identity
Score > 22 indicates homology
RDGNVTVNMGIPVLEPGR + Deamidated (NQ)
3312 1068.5608 2135.1070 2135.1409 -15.8 1 11 0.82 +1Score > 38 indicates identity
Score > 23 indicates homology
LADGAIDLYAMVVVLSRASR + Oxidation (M)
1999 718.3782 1434.7418 1434.7354 4.47 1 11 0.28 +1Score > 41 indicates identity
Score > 18 indicates homology
LQNQVLDSKNFK + 2 Deamidated (NQ)
2783 908.4413 1814.8680 1814.8834 -8.46 0 11 0.65 +1Score > 40 indicates identity
Score > 22 indicates homology
MNIIINMLGFNIFIE + 2 Deamidated (NQ); 2 Oxidation (M)
448 364.7201 727.4256 727.4116 19.3 0 11 0.98 +1Score > 39 indicates identity
Score > 23 indicates homology
VGPTILE
819 449.2811 896.5476 896.5331 16.2 0 11 0.8 +1Score > 34 indicates identity
Score > 23 indicates homology
IKPQAVIE
1805 672.3986 1342.7826 1342.7820 0.48 1 11 0.67 +1Score > 36 indicates identity
Score > 22 indicates homology
TSLLRAVQQVVK + 2 Deamidated (NQ)
2246 779.8892 1557.7638 1557.7674 -2.31 1 11 0.45 +1Score > 40 indicates identity
Score > 20 indicates homology
TNTIPNWIKLNNE + 2 Deamidated (NQ)
1082 502.3012 1002.5878 1002.5710 16.9 0 11 0.52 +1Score > 36 indicates identity
Score > 21 indicates homology
ITGSLTLNGK
2138 752.4175 1502.8204 1502.8358 -10.2 1 11 0.47 +1Score > 38 indicates identity
Score > 20 indicates homology
HPVTPWGKPTKGAK
2157 505.2852 1512.8338 1512.8035 20.0 1 11 0.63 +1Score > 38 indicates identity
Score > 22 indicates homology
DVSSKLNPTLVNPK + 2 Deamidated (NQ)
2637 578.3585 1732.0537 1732.0359 10.3 1 11 1 +1Score > 25 indicates identity
Score > 24 indicates homology
VIKTQNLAALLHAIAR + Deamidated (NQ)
3173 687.3583 2059.0531 2059.0772 -11.7 1 11 0.78 +1Score > 39 indicates identity
Score > 22 indicates homology
TLNLPNTTFPMKANLVQR + 2 Deamidated (NQ)
3674 775.4246 2323.2520 2323.2158 15.6 1 11 0.8 +1Score > 35 indicates identity
Score > 23 indicates homology
QVINGLLANNDITLPKALIDGE + 3 Deamidated (NQ)
1372 573.8172 1145.6198 1145.6114 7.36 0 11 0.95 +1Score > 40 indicates identity
Score > 23 indicates homology
IDIMISDALR
1930 468.5827 1402.7263 1402.7279 -1.14 0 11 0.5 +1Score > 41 indicates identity
Score > 21 indicates homology
DVMTYLGHLGGIK
2793 456.7447 1822.9497 1822.9532 -1.95 1 11 0.51 +1Score > 39 indicates identity
Score > 21 indicates homology
SMLAVTGVNAGGKTMLLK + Deamidated (NQ); 2 Oxidation (M)
3365 1081.0698 2160.1250 2160.0838 19.1 1 11 0.67 +1Score > 38 indicates identity
Score > 22 indicates homology
GAEVFVIQSISEPVNDTILE + Deamidated (NQ)
3258 706.0328 2115.0766 2115.0524 11.4 1 11 0.35 +1Score > 39 indicates identity
Score > 19 indicates homology
GSNLEFALGYSHPVLITAPE + Deamidated (NQ)
3637 1153.5551 2305.0956 2305.1122 -7.19 1 11 0.15 +1Score > 39 indicates identity
Score > 15 indicates homology
MNNLIKAYAAGVMSAAFLFGSE + Deamidated (NQ)
1380 384.1891 1149.5455 1149.5448 0.57 1 11 0.66 +1Score > 41 indicates identity
Score > 22 indicates homology
MSVAKVDDNR + Oxidation (M)
2257 781.4413 1560.8680 1560.8399 18.0 0 11 0.84 +1Score > 37 indicates identity
Score > 23 indicates homology
QFSLLLADTVLVNE
3046 661.6740 1982.0002 1982.0084 -4.13 1 11 0.61 +1Score > 39 indicates identity
Score > 21 indicates homology
AWKMFTIAQANVRPGYK + 2 Deamidated (NQ)
3707 1175.5757 2349.1368 2349.1812 -18.9 1 11 0.68 +1Score > 39 indicates identity
Score > 22 indicates homology
TNPNFNQSVLNTALKTNSSGIK + 2 Deamidated (NQ)
1633 418.8839 1253.6299 1253.6503 -16.3 1 11 0.59 +1Score > 40 indicates identity
Score > 21 indicates homology
SKSVDSQIYVK + Deamidated (NQ)
2620 863.9917 1725.9688 1725.9665 1.37 1 11 0.71 +1Score > 35 indicates identity
Score > 22 indicates homology
ALIQAWQKDGILQIK + 2 Deamidated (NQ)
2936 957.5425 1913.0704 1913.0544 8.41 0 11 0.98 +1Score > 34 indicates identity
Score > 23 indicates homology
IISGMGVGGIAVLSPTLISE
4056 896.1061 2685.2965 2685.2956 0.34 0 11 0.32 +1Score > 38 indicates identity
Score > 18 indicates homology
APNIDGSDLHSAVIITWINMSTSNK + 2 Deamidated (NQ)
4086 903.4857 2707.4353 2707.4268 3.12 1 11 0.23 +1Score > 35 indicates identity
Score > 17 indicates homology
WIGSVNSIGMAAGAFLFGLLADRIGR + Oxidation (M)
2696 884.9349 1767.8552 1767.8726 -9.83 1 11 0.61 +1Score > 40 indicates identity
Score > 21 indicates homology
GSNLRFMTWDVALSR + Oxidation (M)
2051 732.3920 1462.7694 1462.7569 8.61 0 11 0.56 +1Score > 40 indicates identity
Score > 21 indicates homology
TDALQGYQWLLR
2457 548.2964 1641.8674 1641.8587 5.29 1 11 0.51 +1Score > 40 indicates identity
Score > 20 indicates homology
SGAFIPGIRPGEQSAR
3604 1141.5972 2281.1798 2281.2066 -11.7 0 11 0.4 +1Score > 38 indicates identity
Score > 19 indicates homology
SAVADGPHSVILNQVTYGIAIR + Deamidated (NQ)
1832 677.8810 1353.7474 1353.7616 -10.5 1 11 0.59 +1Score > 38 indicates identity
Score > 21 indicates homology
DLLKVIGSRPQE
2456 821.4647 1640.9148 1640.9171 -1.38 1 11 0.86 +1Score > 37 indicates identity
Score > 23 indicates homology
AGIGMLEGVLSLIPGAK + Oxidation (M)
2624 576.9601 1727.8585 1727.8512 4.19 0 11 0.48 +1Score > 40 indicates identity
Score > 20 indicates homology
LPVSMPSGNQQPSLTR + Deamidated (NQ); Oxidation (M)
3182 692.3567 2074.0483 2074.0219 12.7 1 11 0.98 +1Score > 39 indicates identity
Score > 23 indicates homology
VVFNQDEDGILDIALQGAR + 2 Deamidated (NQ)
482 744.4554 743.4481 743.4429 7.02 0 11 0.2 +1Score > 40 indicates identity
Score > 16 indicates homology
LIDLTVA
1243 543.7737 1085.5328 1085.5512 -16.9 1 11 0.52 +1Score > 40 indicates identity
Score > 20 indicates homology
CRPNVAGRR + Deamidated (NQ)
3385 724.7315 2171.1727 2171.1741 -0.64 0 11 0.45 +1Score > 36 indicates identity
Score > 20 indicates homology
YLPLVATLGLFIFFANLME
1572 616.3484 1230.6822 1230.6932 -8.90 1 11 0.73 +1Score > 39 indicates identity
Score > 22 indicates homology
LDKVVTGVASSR
1794 668.8312 1335.6478 1335.6531 -3.95 1 11 0.85 +1Score > 41 indicates identity
Score > 23 indicates homology
TDQYRQVSSPR
1970 712.3593 1422.7040 1422.6813 16.0 1 11 0.61 +1Score > 41 indicates identity
Score > 21 indicates homology
IENCNYAIQLGK + Deamidated (NQ)
3283 709.3901 2125.1485 2125.1313 8.06 1 11 0.54 +1Score > 36 indicates identity
Score > 21 indicates homology
RNLGDLCGLQVLLIGAGGAAR + 2 Deamidated (NQ)
3047 662.0055 1982.9947 1982.9850 4.88 0 11 0.28 +1Score > 40 indicates identity
Score > 18 indicates homology
VNFAVQAFAALNSNNFVR + 2 Deamidated (NQ)
3112 677.3561 2029.0465 2029.0224 11.9 0 11 0.24 +1Score > 39 indicates identity
Score > 17 indicates homology
IPGMPGANIGVLNISDMLGK + Deamidated (NQ); 2 Oxidation (M)
3268 530.2804 2117.0925 2117.0905 0.92 0 11 0.57 +1Score > 39 indicates identity
Score > 21 indicates homology
QYIAQHFVAISTNANDVVK
3676 775.4283 2323.2631 2323.2466 7.10 1 11 0.9 +1Score > 34 indicates identity
Score > 23 indicates homology
RLMGLVLIMLSTQMFLDGIR + Deamidated (NQ); Oxidation (M)
3705 783.4288 2347.2646 2347.2933 -12.3 1 11 0.36 +1Score > 35 indicates identity
Score > 19 indicates homology
AQEPVKGPVSTKPGSCPIILIR + Deamidated (NQ)
3074 668.0253 2001.0541 2001.0565 -1.20 1 11 0.29 +1Score > 39 indicates identity
Score > 18 indicates homology
DTLIVSAVTPDLNMTRQK
3242 703.0446 2106.1120 2106.0898 10.5 1 11 0.34 +1Score > 37 indicates identity
Score > 18 indicates homology
QADVALIWYFTRPQLER + Deamidated (NQ)
1761 661.7982 1321.5818 1321.6051 -17.6 1 11 0.84 +1Score > 39 indicates identity
Score > 22 indicates homology
WGNGVYGAEAAAR + Deamidated (NQ)
3460 550.2762 2197.0757 2197.0362 18.0 1 11 0.95 +1Score > 39 indicates identity
Score > 23 indicates homology
GCVVNSNAVVGSEGFGFVPTAK + 2 Deamidated (NQ)
2733 597.3096 1788.9070 1788.9192 -6.85 1 11 0.22 +1Score > 40 indicates identity
Score > 17 indicates homology
NAPAIFQRMIDDILR + Deamidated (NQ); Oxidation (M)
2875 938.9548 1875.8950 1875.8950 0.039 1 11 0.82 +1Score > 40 indicates identity
Score > 22 indicates homology
QIEVTTDGAGVSGTVVDVE + Deamidated (NQ)
3065 666.0639 1995.1699 1995.1631 3.40 0 11 0.1 +1Score > 28 indicates identity
Score > 13 indicates homology
IPAGLLVMLLLWLGYLNP
1102 506.7426 1011.4706 1011.4695 1.12 1 11 0.74 +1Score > 40 indicates identity
Score > 22 indicates homology
MAVDIEYR + Oxidation (M)
1544 610.8376 1219.6606 1219.6561 3.77 1 11 0.53 +1Score > 40 indicates identity
Score > 20 indicates homology
ALQGIFNSKNK + Deamidated (NQ)
2897 472.2731 1885.0633 1885.0933 -15.9 1 11 0.51 +1Score > 34 indicates identity
Score > 20 indicates homology
KLMLIFDSILLLAHMK
3061 996.0192 1990.0238 1990.0081 7.92 1 11 0.57 +1Score > 39 indicates identity
Score > 21 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
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