MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1617 312.4472 1245.7597 1245.7445 12.2 0 14 0.41 +1Score > 29 indicates identity
Score > 23 indicates homology		 ITVGLTVFAIGR
2218 515.6046 1543.7920 1543.7630 18.7 0 14 0.42 +1Score > 41 indicates identity
Score > 23 indicates homology		 INTQFADLITHNR + 2 Deamidated (NQ)
2369 537.3431 1609.0075 1608.9814 16.2 1 14 0.28 +1Score > 21 indicates identity LSPAEVALITTLLLR
1365 572.3234 1142.6322 1142.6481 -13.9 1 14 0.82 +1Score > 41 indicates identity
Score > 26 indicates homology		 IAMQAKVPIR + Deamidated (NQ); Oxidation (M)
1429 391.2053 1170.5941 1170.5815 10.7 1 14 0.72 +1Score > 40 indicates identity
Score > 25 indicates homology		 NMHLAATELR + Oxidation (M)
784 442.2675 882.5204 882.5287 -9.32 1 14 0.92 +1Score > 37 indicates identity
Score > 26 indicates homology		 IDPLAARK
2136 752.3931 1502.7716 1502.7437 18.6 1 14 0.72 +1Score > 41 indicates identity
Score > 25 indicates homology		 LTSDGAGISGGQRQR + Deamidated (NQ)
2146 378.7179 1510.8425 1510.8157 17.7 1 14 0.33 +1Score > 37 indicates identity
Score > 22 indicates homology		 LFWLAHATSPRGR
2902 944.5087 1887.0028 1887.0101 -3.87 1 14 0.89 +1Score > 38 indicates identity
Score > 26 indicates homology		 NFGIGADGIILILESNNK
1162 346.8506 1037.5300 1037.5115 17.8 0 14 0.47 +1Score > 40 indicates identity
Score > 23 indicates homology		 GGPGGGRPGGGGR
3155 684.6955 2051.0647 2051.0431 10.5 1 14 0.47 +1Score > 39 indicates identity
Score > 23 indicates homology		 KLGFVGSGQGIMAQAVVLME + Deamidated (NQ); Oxidation (M)
1871 690.3367 1378.6588 1378.6802 -15.5 0 14 0.37 +1Score > 41 indicates identity
Score > 22 indicates homology		 MSGFQINLNPLK + 2 Deamidated (NQ); Oxidation (M)
3217 1047.5369 2093.0592 2093.0252 16.3 0 14 0.17 +1Score > 39 indicates identity
Score > 19 indicates homology		 MSDAVPQVGSQFGPYQLLR + Deamidated (NQ)
3172 1030.5333 2059.0520 2059.0884 -17.7 1 14 0.35 +1Score > 39 indicates identity
Score > 22 indicates homology		 ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
2470 825.9300 1649.8454 1649.8334 7.28 1 14 0.75 +1Score > 40 indicates identity
Score > 25 indicates homology		 AVLLMAAFELTQQAE + Oxidation (M)
398 351.7120 701.4094 701.4072 3.23 0 14 0.74 +1Score > 43 indicates identity
Score > 25 indicates homology		 IIDSVR
2393 538.9551 1613.8435 1613.8334 6.24 1 14 0.26 +1Score > 40 indicates identity
Score > 21 indicates homology		 KLNINLAMSPLPSSE + Deamidated (NQ)
2500 834.9479 1667.8812 1667.9094 -16.9 1 14 0.48 +1Score > 39 indicates identity
Score > 23 indicates homology		 ADTQKALVDLAPNLAK + Deamidated (NQ)
1225 538.7970 1075.5794 1075.5662 12.3 1 14 0.84 +1Score > 41 indicates identity
Score > 26 indicates homology		 VPLQDGRYK + Deamidated (NQ)
1378 383.8700 1148.5882 1148.6077 -17.0 0 14 0.46 +1Score > 42 indicates identity
Score > 23 indicates homology		 QFQQVLASVK + 2 Deamidated (NQ)
2219 772.9113 1543.8080 1543.7981 6.48 0 14 0.18 +1Score > 40 indicates identity
Score > 19 indicates homology		 LAIQNSQIASAIALE + 3 Deamidated (NQ)
1148 344.8671 1031.5795 1031.5611 17.8 1 14 0.55 +1Score > 40 indicates identity
Score > 24 indicates homology		 KNVVNSGVSK + Deamidated (NQ)
1643 421.5735 1261.6987 1261.6886 7.96 1 14 0.63 +1Score > 39 indicates identity
Score > 24 indicates homology		 VDKIILQMMR + Oxidation (M)
1233 541.8062 1081.5978 1081.6066 -8.12 1 14 0.23 +1Score > 38 indicates identity
Score > 20 indicates homology		 APGVGPKVAMR
3204 1044.0072 2085.9998 2085.9816 8.75 1 14 0.21 +1Score > 39 indicates identity
Score > 20 indicates homology		 ALDTYNEINAVMNLVLFE + 2 Deamidated (NQ); Oxidation (M)
1367 572.7966 1143.5786 1143.5713 6.45 0 14 0.98 +1Score > 41 indicates identity
Score > 26 indicates homology		 YASALYGPFR
2133 752.3762 1502.7378 1502.7253 8.36 1 14 0.56 +1Score > 41 indicates identity
Score > 24 indicates homology		 GESFGAAISQPITPE
513 382.2098 762.4050 762.4163 -14.8 1 14 0.86 +1Score > 43 indicates identity
Score > 26 indicates homology		 ILFEIE
1839 679.8647 1357.7148 1357.6990 11.7 1 14 0.93 +1Score > 41 indicates identity
Score > 26 indicates homology		 NPIFSIPNGKNR + 2 Deamidated (NQ)
832 301.5159 901.5259 901.5345 -9.57 1 14 0.88 +1Score > 39 indicates identity
Score > 26 indicates homology		 RSGITLAGK
582 395.7095 789.4044 789.4167 -15.5 1 14 0.65 +1Score > 42 indicates identity
Score > 24 indicates homology		 KADVMAR
1836 679.3247 1356.6348 1356.6534 -13.7 1 14 0.91 +1Score > 40 indicates identity
Score > 26 indicates homology		 NPDNATWNRIR + Deamidated (NQ)
1943 705.3973 1408.7800 1408.7926 -8.88 1 14 0.62 +1Score > 37 indicates identity
Score > 24 indicates homology		 VIGEALNQLVPQK + Deamidated (NQ)
2805 610.6906 1829.0500 1829.0159 18.6 1 14 0.46 +1Score > 32 indicates identity
Score > 23 indicates homology		 QSQAASVFQALPSKLVR
4156 929.5132 2785.5178 2785.5059 4.28 0 14 1 +1Score > 32 indicates identity
Score > 26 indicates homology		 MLPVLFVTFVPSMMLAQIMTLFIK + Oxidation (M)
2013 722.3766 1442.7386 1442.7477 -6.29 1 14 0.9 +1Score > 41 indicates identity
Score > 26 indicates homology		 QAISQQKQVQQR + 2 Deamidated (NQ)
1432 391.5692 1171.6858 1171.6925 -5.71 1 14 0.46 +1Score > 37 indicates identity
Score > 23 indicates homology		 KLVTAAVQSVR + Deamidated (NQ)
2781 605.6303 1813.8691 1813.8992 -16.6 1 14 0.37 +1Score > 40 indicates identity
Score > 22 indicates homology		 SHEPKSPSLGMISTATR + Oxidation (M)
1036 492.2998 982.5850 982.5811 3.99 1 14 0.62 +1Score > 36 indicates identity
Score > 24 indicates homology		 DKGAVVAPVK
3288 1064.5636 2127.1126 2127.0749 17.7 1 14 0.76 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
2193 766.8930 1531.7714 1531.7916 -13.2 0 14 0.43 +1Score > 41 indicates identity
Score > 22 indicates homology		 IVINMGVGDAVTNTK + Deamidated (NQ)
3053 994.5129 1987.0112 1987.0098 0.75 1 14 0.76 +1Score > 39 indicates identity
Score > 25 indicates homology		 LAMLSTHYRQPINWTR + Deamidated (NQ)
411 354.6890 707.3634 707.3602 4.55 0 14 0.48 +1Score > 41 indicates identity
Score > 23 indicates homology		 FIADSR
1533 608.8149 1215.6152 1215.6347 -16.0 1 14 0.74 +1Score > 42 indicates identity
Score > 25 indicates homology		 DNAVLSVEQIK + Deamidated (NQ)
701 426.2133 850.4120 850.3967 18.1 1 14 0.72 +1Score > 42 indicates identity
Score > 25 indicates homology		 SAMDKGAR + Oxidation (M)
1760 661.4163 1320.8180 1320.8017 12.4 0 14 1 +1Score > 28 indicates identity
Score > 26 indicates homology		 VSVPLPVDVVVAK
694 425.2279 848.4412 848.4392 2.42 1 14 0.61 +1Score > 42 indicates identity
Score > 24 indicates homology		 ALKANGFE
2447 546.3292 1635.9658 1635.9712 -3.31 1 14 0.66 +1Score > 30 indicates identity
Score > 24 indicates homology		 GLVLSLPELLGWAIR
2628 576.9918 1727.9536 1727.9206 19.1 1 14 0.54 +1Score > 37 indicates identity
Score > 23 indicates homology		 FNTLHQLLRTLQDK + 2 Deamidated (NQ)
2938 638.9902 1913.9488 1913.9807 -16.7 1 14 0.59 +1Score > 40 indicates identity
Score > 24 indicates homology		 SSASVVEAVSVNSAIPQNR
1069 499.8001 997.5856 997.5808 4.89 0 14 0.56 +1Score > 38 indicates identity
Score > 24 indicates homology		 VSAIQPAIAK + Deamidated (NQ)
350 341.6990 681.3834 681.3810 3.64 0 14 0.79 +1Score > 37 indicates identity
Score > 25 indicates homology		 IDVHAK
1843 454.5750 1360.7032 1360.6908 9.11 1 14 0.51 +1Score > 41 indicates identity
Score > 23 indicates homology		 EASQLLACDILK + Deamidated (NQ)
3023 986.0253 1970.0360 1970.0360 0.011 0 14 0.62 +1Score > 38 indicates identity
Score > 24 indicates homology		 DLGSALNPFAAQQLILGLE + Deamidated (NQ)
1070 500.2743 998.5340 998.5185 15.6 1 14 0.93 +1Score > 40 indicates identity
Score > 26 indicates homology		 WQIDRLAP + Deamidated (NQ)
1026 488.7852 975.5558 975.5614 -5.66 1 14 0.9 +1Score > 39 indicates identity
Score > 26 indicates homology		 LYQQRIR
1101 506.7424 1011.4702 1011.4695 0.74 1 14 0.32 +1Score > 40 indicates identity
Score > 21 indicates homology		 VLYMENSR + Deamidated (NQ)
1095 337.5272 1009.5598 1009.5597 0.11 0 14 0.51 +1Score > 37 indicates identity
Score > 23 indicates homology		 FFSLDILR
1846 682.3280 1362.6414 1362.6375 2.87 1 14 0.77 +1Score > 41 indicates identity
Score > 25 indicates homology		 SGDVQNDGTKSVR + Deamidated (NQ)
2851 932.4612 1862.9078 1862.8898 9.70 0 14 0.28 +1Score > 40 indicates identity
Score > 20 indicates homology		 AIVSLQPGQDLDFSSSAE
2920 951.0227 1900.0308 1900.0629 -16.9 1 14 0.39 +1Score > 37 indicates identity
Score > 22 indicates homology		 VLNSKVTALGNTILTNNK + Deamidated (NQ)
2931 478.5028 1909.9821 1909.9686 7.05 1 14 0.51 +1Score > 39 indicates identity
Score > 23 indicates homology		 FLKINWDQTFNQLSR + Deamidated (NQ)
320 336.1929 670.3712 670.3762 -7.39 0 13 0.87 +1Score > 36 indicates identity
Score > 25 indicates homology		 AGLPSAR
1957 709.8853 1417.7560 1417.7816 -18.1 1 13 0.85 +1Score > 40 indicates identity
Score > 25 indicates homology		 YPVVSAASIIAKAE
220 307.7095 613.4044 613.4024 3.41 1 13 0.25 +1Score > 39 indicates identity
Score > 20 indicates homology		 RVAIR
2321 793.9314 1585.8482 1585.8311 10.8 1 13 0.59 +1Score > 39 indicates identity
Score > 24 indicates homology		 QAITNPNTIQSIKR + 3 Deamidated (NQ)
2666 586.9986 1757.9740 1757.9828 -5.05 0 13 0.9 +1Score > 36 indicates identity
Score > 25 indicates homology		 VSVIPTLLHVAGYSFR
1567 410.5768 1228.7086 1228.7139 -4.35 1 13 0.45 +1Score > 38 indicates identity
Score > 22 indicates homology		 QAAKSVVASGLAK
2062 735.9184 1469.8222 1469.8089 9.06 0 13 0.24 +1Score > 37 indicates identity
Score > 20 indicates homology		 LTSLLNLNQVPTR + 2 Deamidated (NQ)
2177 508.2604 1521.7594 1521.7358 15.5 0 13 0.57 +1Score > 41 indicates identity
Score > 23 indicates homology		 MHINGSNVQVLHR + 2 Deamidated (NQ); Oxidation (M)
2911 631.6655 1891.9747 1892.0043 -15.7 1 13 0.68 +1Score > 39 indicates identity
Score > 24 indicates homology		 EPPAHVKFLLATTDPQK + Deamidated (NQ)
1379 575.7781 1149.5416 1149.5448 -2.73 1 13 0.5 +1Score > 41 indicates identity
Score > 23 indicates homology		 LMNQSQGSRK + 2 Deamidated (NQ)
1883 347.4503 1385.7721 1385.7779 -4.21 1 13 0.28 +1Score > 38 indicates identity
Score > 20 indicates homology		 LPQVRVLDYQR
2649 581.6682 1741.9828 1741.9800 1.58 1 13 0.13 +1Score > 34 indicates identity
Score > 17 indicates homology		 ALLENIALYLTHMLK
3194 1040.0552 2078.0958 2078.0983 -1.16 0 13 0.81 +1Score > 38 indicates identity
Score > 25 indicates homology		 LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ)
2576 569.9531 1706.8375 1706.8264 6.50 1 13 0.28 +1Score > 41 indicates identity
Score > 20 indicates homology		 RQTFALFSNPGNPQK + 3 Deamidated (NQ)
3254 1058.0433 2114.0720 2114.0684 1.72 1 13 0.33 +1Score > 39 indicates identity
Score > 21 indicates homology		 AVVDKWPDFNTVVIRPQE + 2 Deamidated (NQ)
3641 769.7101 2306.1085 2306.1464 -16.4 1 13 0.27 +1Score > 39 indicates identity
Score > 20 indicates homology		 FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
1982 714.3923 1426.7700 1426.7490 14.8 1 13 0.11 +1Score > 39 indicates identity
Score > 16 indicates homology		 VQAALADCLKLPE
2619 576.3167 1725.9283 1725.9600 -18.4 1 13 0.51 +1Score > 38 indicates identity
Score > 23 indicates homology		 LTFGTVLMGLSRLFR + Oxidation (M)
1966 474.6095 1420.8067 1420.8289 -15.7 1 13 0.26 +1Score > 35 indicates identity
Score > 20 indicates homology		 SLYDILLTRLSK
2957 641.6871 1922.0395 1922.0338 2.98 1 13 0.43 +1Score > 36 indicates identity
Score > 22 indicates homology		 MTRCALLLLMVLMLGR + 2 Oxidation (M)
3634 577.0300 2304.0909 2304.0692 9.43 1 13 0.81 +1Score > 39 indicates identity
Score > 25 indicates homology		 QSLDYANQAIDAMEHILQSR + 2 Deamidated (NQ)
1585 619.3145 1236.6144 1236.6067 6.29 1 13 0.82 +1Score > 41 indicates identity
Score > 25 indicates homology		 MNSNRMLALR + 2 Oxidation (M)
203 301.6984 601.3822 601.3799 3.88 0 13 0.69 +1Score > 41 indicates identity
Score > 24 indicates homology		 LVSVGK
3086 671.0223 2010.0451 2010.0520 -3.47 0 13 0.51 +1Score > 39 indicates identity
Score > 23 indicates homology		 VSIQQILGNNDEPLINLK + 3 Deamidated (NQ)
2024 725.3759 1448.7372 1448.7624 -17.3 0 13 0.91 +1Score > 41 indicates identity
Score > 25 indicates homology		 QVTFLGTSSGVPTR
1662 638.8485 1275.6824 1275.7009 -14.5 1 13 0.44 +1Score > 41 indicates identity
Score > 22 indicates homology		 LMSKILGSFHK + Oxidation (M)
1906 695.8929 1389.7712 1389.7980 -19.2 1 13 0.85 +1Score > 38 indicates identity
Score > 25 indicates homology		 YQKGILVQALTR + Deamidated (NQ)
2812 920.0087 1838.0028 1837.9897 7.14 1 13 0.067 +1Score > 36 indicates identity
Score > 14 indicates homology		 AQLVAEALSPGANVSAIAR + Deamidated (NQ)
594 398.2042 794.3938 794.3922 2.02 1 13 0.33 +1Score > 41 indicates identity
Score > 21 indicates homology		 LSQEYR
2230 517.6126 1549.8160 1549.8062 6.34 1 13 0.77 +1Score > 40 indicates identity
Score > 25 indicates homology		 AGLMEIADIFVINK + Deamidated (NQ); Oxidation (M)
1711 649.3766 1296.7386 1296.7150 18.3 1 13 0.5 +1Score > 37 indicates identity
Score > 23 indicates homology		 NNLVIDTPRVR + Deamidated (NQ)
1879 462.6079 1384.8019 1384.7926 6.70 0 13 0.97 +1Score > 36 indicates identity
Score > 26 indicates homology		 SILGNIVGGDLVTK
2530 558.6528 1672.9366 1672.9359 0.38 1 13 0.51 +1Score > 36 indicates identity
Score > 23 indicates homology		 LVQSQLLKSVVSGSAR + 2 Deamidated (NQ)
1001 485.7656 969.5166 969.5244 -7.95 0 13 0.67 +1Score > 39 indicates identity
Score > 24 indicates homology		 VDPVSIQGR
850 304.5006 910.4800 910.4946 -16.1 1 13 0.68 +1Score > 38 indicates identity
Score > 24 indicates homology		 KCYSLIK
1272 550.8038 1099.5930 1099.6026 -8.66 1 13 0.45 +1Score > 41 indicates identity
Score > 22 indicates homology		 ISPALFEAPR
3646 578.0336 2308.1053 2308.0933 5.19 1 13 0.069 +1Score > 39 indicates identity
Score > 14 indicates homology		 FIILSDILGDEDHLGDMDFK + Oxidation (M)
4179 934.7950 2801.3632 2801.3389 8.67 1 13 0.21 +1Score > 38 indicates identity
Score > 19 indicates homology		 SLGGSPSNGQAPLTDINMLATAATQVER + 3 Deamidated (NQ)
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