MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3818)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
196 601.3693 600.3620 600.3595 4.18 0 44 0.058 +1Score > 44 indicates identity AGAGVVK
688 423.2294 844.4442 844.4290 18.0 0 41 0.088 +1Score > 43 indicates identity QAALQADK + Deamidated (NQ)
797 446.2318 890.4490 890.4458 3.70 1 40 0.078 +1Score > 42 indicates identity
Score > 41 indicates homology		 TGTLTENR
805 447.7392 893.4638 893.4606 3.58 1 40 0.082 +1Score > 41 indicates identity SLLNEYR
509 761.4069 760.3996 760.4079 -10.9 0 40 0.12 +1Score > 43 indicates identity SGNATALK
732 430.7234 859.4322 859.4174 17.2 1 39 0.14 +1Score > 43 indicates identity IIQENIE + 2 Deamidated (NQ)
1640 631.3552 1260.6958 1260.7190 -18.4 1 39 0.058 +1Score > 40 indicates identity KFLLQTGVQAR + Deamidated (NQ)
334 337.2172 672.4198 672.4170 4.20 0 39 0.073 +1Score > 40 indicates identity SGGVIIK
1377 575.3009 1148.5872 1148.5747 10.9 1 39 0.11 +1Score > 42 indicates identity KQIQQMVQK + 3 Deamidated (NQ); Oxidation (M)
767 438.2483 874.4820 874.4760 6.92 0 38 0.1 +1Score > 41 indicates identity TLQDLGTK
485 374.2305 746.4464 746.4439 3.42 0 38 0.059 +1Score > 38 indicates identity IQALFR
724 429.7581 857.5016 857.4858 18.5 1 38 0.16 +1Score > 42 indicates identity QQLVIEK + Deamidated (NQ)
272 643.4155 642.4082 642.4064 2.76 0 37 0.14 +1Score > 41 indicates identity NVAVLK
1328 1131.5669 1130.5596 1130.5567 2.54 1 37 0.15 +1Score > 42 indicates identity VVELNASDQR + Deamidated (NQ)
169 572.3785 571.3712 571.3693 3.33 0 37 0.12 +1Score > 40 indicates identity AAAVIK
1044 494.7958 987.5770 987.5674 9.76 1 37 0.077 +1Score > 38 indicates identity MELILLQK + Deamidated (NQ)
511 761.4547 760.4474 760.4443 4.12 0 37 0.078 +1Score > 38 indicates identity TTIASLR
1298 560.2789 1118.5432 1118.5455 -2.04 0 37 0.082 +1Score > 42 indicates identity
Score > 38 indicates homology		 AGVTNDSQVVK + 2 Deamidated (NQ)
1639 631.3546 1260.6946 1260.6747 15.8 1 37 0.11 +1Score > 40 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
931 471.7737 941.5328 941.5294 3.64 0 37 0.1 +1Score > 39 indicates identity GAGGLADLIR
166 565.3367 564.3294 564.3271 4.03 0 36 0.08 +1Score > 38 indicates identity TVFAK
1995 477.9259 1430.7559 1430.7551 0.53 1 36 0.087 +1Score > 40 indicates identity
Score > 38 indicates homology		 AGQNLAKTLQMVR + 2 Deamidated (NQ)
1091 505.2539 1008.4932 1008.5128 -19.3 1 36 0.23 +1Score > 42 indicates identity QTLDYIEK
1358 571.8094 1141.6042 1141.6091 -4.25 1 35 0.18 +1Score > 40 indicates identity LNELQQLGAR + Deamidated (NQ)
289 651.3237 650.3164 650.3136 4.30 0 35 0.13 +1Score > 43 indicates identity
Score > 39 indicates homology		 QGFSGR
1077 501.2872 1000.5598 1000.5553 4.56 1 35 0.24 +1Score > 41 indicates identity QATLEQALK
1286 556.8284 1111.6422 1111.6349 6.59 1 35 0.066 +1Score > 38 indicates identity
Score > 35 indicates homology		 ALAAALGQIER
743 433.7475 865.4804 865.4770 4.01 1 35 0.12 +1Score > 38 indicates identity AIHEVAAR
1318 564.8267 1127.6388 1127.6298 8.00 0 34 0.097 +1Score > 40 indicates identity
Score > 37 indicates homology		 LLNLSLANNR + Deamidated (NQ)
300 329.7114 657.4082 657.4061 3.25 0 34 0.19 +1Score > 41 indicates identity
Score > 40 indicates homology		 IDVALK
1015 487.2626 972.5106 972.5128 -2.17 0 34 0.062 +1Score > 43 indicates identity
Score > 35 indicates homology		 AISIVNAVGE + Deamidated (NQ)
574 394.7364 787.4582 787.4440 18.1 0 34 0.31 +1Score > 42 indicates identity LGGTIIQS
242 314.6756 627.3366 627.3340 4.22 1 34 0.12 +1Score > 37 indicates identity IENPR
347 682.3455 681.3382 681.3333 7.19 0 34 0.13 +1Score > 38 indicates identity NAYSVK + Deamidated (NQ)
759 437.2632 872.5118 872.5080 4.47 1 34 0.18 +1Score > 40 indicates identity
Score > 39 indicates homology		 KGNVNLTK
322 336.2271 670.4396 670.4377 2.84 0 34 0.11 +1Score > 37 indicates identity VAGLAIK
597 398.7155 795.4164 795.4014 18.9 0 34 0.16 +1Score > 39 indicates identity AVTTYLE
535 386.7394 771.4642 771.4490 19.7 0 34 0.064 +1Score > 42 indicates identity
Score > 35 indicates homology		 AVVVAANK + Deamidated (NQ)
627 407.7533 813.4920 813.5072 -18.6 0 34 0.18 +1Score > 41 indicates identity
Score > 39 indicates homology		 NIILVSR
1510 604.7838 1207.5530 1207.5754 -18.5 1 34 0.16 +1Score > 41 indicates identity
Score > 39 indicates homology		 KDLTTSMNAVE
1749 660.3537 1318.6928 1318.6802 9.57 1 34 0.15 +1Score > 41 indicates identity
Score > 38 indicates homology		 NQISIMDLIEK + Oxidation (M)
930 471.7735 941.5324 941.5294 3.23 0 34 0.074 +1Score > 39 indicates identity
Score > 35 indicates homology		 LAVNAGGLAR + Deamidated (NQ)
1521 606.3568 1210.6990 1210.7186 -16.2 1 34 0.063 +1Score > 36 indicates identity
Score > 34 indicates homology		 KWVGRPLIDK
656 414.7472 827.4798 827.4865 -8.00 1 34 0.16 +1Score > 40 indicates identity
Score > 38 indicates homology		 LKASANPK
595 398.2233 794.4320 794.4286 4.30 0 33 0.21 +1Score > 39 indicates identity AVSSYLR
155 544.3492 543.3419 543.3380 7.14 0 33 0.6 +1Score > 44 indicates identity IAGGVK
1072 500.2853 998.5560 998.5760 -20.0 1 33 0.25 +1Score > 40 indicates identity QLQELQLK
380 700.4122 699.4049 699.4028 3.10 0 33 0.27 +1Score > 40 indicates identity AGGILNR
455 365.2304 728.4462 728.4432 4.15 1 33 0.27 +1Score > 42 indicates identity
Score > 40 indicates homology		 VAIEAVK
804 894.4710 893.4637 893.4607 3.43 1 33 0.41 +1Score > 41 indicates identity GISEGLYR
730 430.2553 858.4960 858.4810 17.5 1 33 0.44 +1Score > 42 indicates identity AATENILK
358 344.2250 686.4354 686.4327 4.06 0 33 0.31 +1Score > 40 indicates identity SVGAILK
1187 529.2882 1056.5618 1056.5815 -18.6 1 33 0.39 +1Score > 41 indicates identity LALQNEQLK + Deamidated (NQ)
494 753.4291 752.4218 752.4181 4.98 0 33 0.19 +1Score > 38 indicates identity ALDVHAK
430 360.1782 718.3418 718.3432 -1.88 0 33 0.44 +1Score > 43 indicates identity
Score > 41 indicates homology		 APMSTGR
1073 500.7763 999.5380 999.5349 3.15 0 32 0.096 +1Score > 40 indicates identity
Score > 34 indicates homology		 VANLGNLGDK
1277 553.3091 1104.6036 1104.5928 9.86 1 32 0.077 +1Score > 40 indicates identity
Score > 33 indicates homology		 QIELVFSGGR
1344 567.8279 1133.6412 1133.6557 -12.7 1 32 0.052 +1Score > 38 indicates identity
Score > 32 indicates homology		 RLHPLSLIGE
659 415.7421 829.4696 829.4810 -13.7 1 32 0.77 +1Score > 43 indicates identity KIIDWR
356 344.1941 686.3736 686.3711 3.70 1 32 0.61 +1Score > 43 indicates identity
Score > 42 indicates homology		 IAAEAGR
1146 516.2626 1030.5106 1030.4931 17.0 0 32 0.62 +1Score > 42 indicates identity LTQNLAGGQE + Deamidated (NQ)
692 424.7001 847.3856 847.3937 -9.46 1 32 0.081 +1Score > 41 indicates identity
Score > 33 indicates homology		 DDTWRR
769 877.5167 876.5094 876.4925 19.3 1 32 0.081 +1Score > 38 indicates identity
Score > 33 indicates homology		 KMGMLVAK
1840 453.5822 1357.7248 1357.7201 3.44 1 32 0.088 +1Score > 40 indicates identity
Score > 34 indicates homology		 QTALSIEQQLAR + Deamidated (NQ)
463 366.7048 731.3950 731.3813 18.7 1 32 0.13 +1Score > 44 indicates identity
Score > 35 indicates homology		 TGENALK
483 745.4587 744.4514 744.4494 2.76 0 32 0.38 +1Score > 41 indicates identity
Score > 40 indicates homology		 ITAALTR
1384 576.7722 1151.5298 1151.5260 3.30 1 32 0.088 +1Score > 41 indicates identity
Score > 34 indicates homology		 NYWNGRWR + Deamidated (NQ)
1691 645.3405 1288.6664 1288.6809 -11.2 1 32 0.15 +1Score > 42 indicates identity
Score > 36 indicates homology		 QTILQMGRVDK + Deamidated (NQ)
198 602.3536 601.3463 601.3435 4.66 0 32 0.66 +1Score > 45 indicates identity
Score > 42 indicates homology		 SITGPK
1330 566.2881 1130.5616 1130.5568 4.32 1 31 0.16 +1Score > 42 indicates identity
Score > 36 indicates homology		 VVDGQITNRE + Deamidated (NQ)
596 796.4234 795.4161 795.4014 18.5 0 31 0.3 +1Score > 39 indicates identity SALTYIE
678 421.2633 840.5120 840.5069 6.15 1 31 0.15 +1Score > 35 indicates identity QLKDIPK
302 658.4171 657.4098 657.4061 5.64 0 31 0.58 +1Score > 41 indicates identity DIVAIK
614 802.4460 801.4387 801.4232 19.4 1 31 0.45 -1Score > 43 indicates identity
Score > 40 indicates homology		 QNEIVAK + Deamidated (NQ)
188 598.3945 597.3872 597.3850 3.76 0 31 0.062 +1Score > 31 indicates identity AGILPK
253 315.7016 629.3886 629.3861 4.11 0 31 0.7 +1Score > 42 indicates identity VAVVSR
1559 614.8383 1227.6620 1227.6823 -16.5 0 31 0.49 +1Score > 40 indicates identity IDVGGSAAVLGAAK
338 337.6965 673.3784 673.3871 -12.9 1 31 0.42 +1Score > 42 indicates identity
Score > 39 indicates homology		 GGKSAVR
297 328.7031 655.3916 655.3905 1.82 0 30 0.16 +1Score > 37 indicates identity
Score > 35 indicates homology		 AIDLPK
1637 629.8566 1257.6986 1257.6816 13.6 0 30 0.46 +1Score > 39 indicates identity LVIAVANDSNIK + 2 Deamidated (NQ)
1454 1181.7244 1180.7171 1180.7067 8.81 1 30 0.076 +1Score > 32 indicates identity DVVPVLIEGLK
823 450.2876 898.5606 898.5488 13.2 0 30 0.2 +1Score > 37 indicates identity
Score > 36 indicates homology		 AVLDQLLK
285 324.1673 646.3200 646.3187 2.07 0 30 0.77 +1Score > 44 indicates identity
Score > 42 indicates homology		 GGFNPR
1708 648.8705 1295.7264 1295.7193 5.55 1 30 0.18 +1Score > 37 indicates identity
Score > 35 indicates homology		 MSMLSTLLLKK + 2 Oxidation (M)
1074 500.7959 999.5772 999.5713 5.99 0 30 0.11 +1Score > 40 indicates identity
Score > 33 indicates homology		 SAADIIALAR
912 467.7736 933.5326 933.5171 16.6 0 30 0.42 +1Score > 39 indicates identity VLLANYLE
1561 614.8386 1227.6626 1227.6823 -16.0 0 30 0.59 +1Score > 40 indicates identity QLSQVLNQAVK + Deamidated (NQ)
1010 486.7666 971.5186 971.5148 3.93 0 30 0.081 +1Score > 42 indicates identity
Score > 32 indicates homology		 SAGLQVQNR
1175 522.8004 1043.5862 1043.5863 -0.0096 0 30 0.57 +1Score > 40 indicates identity
Score > 40 indicates homology		 DITIIAVGNK + Deamidated (NQ)
1859 457.5967 1369.7683 1369.7677 0.42 1 30 0.078 +1Score > 38 indicates identity
Score > 31 indicates homology		 NARLSILNAQLR + 2 Deamidated (NQ)
661 416.2466 830.4786 830.4749 4.50 1 30 0.56 +1Score > 40 indicates identity
Score > 40 indicates homology		 TQVELLK + Deamidated (NQ)
700 851.4178 850.4105 850.4072 3.88 1 30 0.46 +1Score > 42 indicates identity
Score > 39 indicates homology		 QIEQFGK + 2 Deamidated (NQ)
942 473.7556 945.4966 945.5131 -17.4 1 30 0.6 +1Score > 42 indicates identity
Score > 40 indicates homology		 TNVGEISVK
328 673.3965 672.3892 672.3806 12.8 0 30 0.68 +1Score > 42 indicates identity
Score > 41 indicates homology		 QIGDLK
691 848.3920 847.3847 847.3937 -10.5 1 30 0.22 +1Score > 41 indicates identity
Score > 36 indicates homology		 DDTWRR
154 544.3480 543.3407 543.3380 4.99 0 30 0.99 +1Score > 44 indicates identity
Score > 42 indicates homology		 AAAAIK
751 435.2420 868.4694 868.4807 -12.9 0 29 0.11 +1Score > 37 indicates identity
Score > 32 indicates homology		 LPWPAGTK
477 371.7470 741.4794 741.4861 -8.93 1 29 0.33 +1Score > 37 indicates identity LAKIAAR
612 801.4995 800.4922 800.4868 6.76 1 29 0.32 +1Score > 37 indicates identity KLGSLQR
913 935.5412 934.5339 934.5276 6.73 0 29 0.12 +1Score > 38 indicates identity
Score > 33 indicates homology		 NFLIPFGK
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