MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 240)


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+91

Accession Score Description
1 CARA_PSEPK 65 Carbamoyl-phosphate synthase small chain OS=Pseudomonas putida (strain KT2440) GN=carA PE=3 SV=1

+92

Accession Score Description
1 BFR_SALTY 64 Bacterioferritin OS=Salmonella typhimurium GN=bfr PE=3 SV=1

+93

Accession Score Description
1 RL14_PSE14 62 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1

+94

Accession Score Description
1 SYS_ALKOO 62 Seryl-tRNA synthetase OS=Alkaliphilus oremlandii (strain OhILAs) GN=serS PE=3 SV=1

+95

Accession Score Description
1 OPRI_PSEAE 61 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+96

Accession Score Description
1 RL13_PSEP1 60 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1

-97

Accession Score Description
1 G6PI1_PSEPK 60 Glucose-6-phosphate isomerase 1 OS=Pseudomonas putida (strain KT2440) GN=pgi1 PE=3 SV=1
Score Mass Matches Sequences emPAI
97.1 G6PI1_PSEPK 60 61573 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase 1 OS=Pseudomonas putida (strain KT2440) GN=pgi1 PE=3 SV=1
6 samesets of G6PI1_PSEPK
G6PI2_PSEPK 60 61644 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase 2 OS=Pseudomonas putida (strain KT2440) GN=pgi2 PE=3 SV=1
G6PI_PSEE4 60 61434 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase OS=Pseudomonas entomophila (strain L48) GN=pgi PE=3 SV=1
G6PI_PSEF5 60 61459 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=pgi PE=3 SV=1
G6PI_PSEP1 60 61617 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pgi PE=3 SV=1
G6PI_PSEPG 60 61629 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase OS=Pseudomonas putida (strain GB-1) GN=pgi PE=3 SV=1
G6PI_PSEPW 60 61847 1 (1) 1 (1) 0.04
Glucose-6-phosphate isomerase OS=Pseudomonas putida (strain W619) GN=pgi PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1607   621.3724 1240.7302 1240.7139 13.2 1 60 3.9e-005 +1Score > 36 indicates identity
Score > 28 indicates homology
U K.VLNQITELVGR.I

+98

Accession Score Description
1 RS4_PSEP1 59 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

+99

Accession Score Description
1 CYOA_PSEPU 59 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+100

Accession Score Description
1 ENO_PSEPK 59 Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1
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