MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)

Page: 1 2 3 4 5 6 … 24 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RPOB_PSEPG 398 151555 29 (16) 26 (16) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
3 samesets of RPOB_PSEPG
RPOB_PSEPK 398 151468 29 (16) 26 (16) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
RPOB_PSEP1 398 151468 29 (16) 26 (16) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
RPOB_PSEPW 398 151379 28 (16) 25 (16) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1
RPOC_PSEE4 267 155376 26 (13) 26 (13) 0.21
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
4 samesets of RPOC_PSEE4
RPOC_PSEPG 267 155435 26 (13) 26 (13) 0.21
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
RPOC_PSEPK 267 155386 26 (13) 26 (13) 0.21
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
RPOC_PSEPW 267 155341 26 (13) 26 (13) 0.21
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain W619) GN=rpoC PE=3 SV=1
RPOC_PSEP1 267 155358 26 (13) 26 (13) 0.21
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
RPOB_SACD2 141 151954 11 (6) 10 (6) 0.09
DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
RPOC_SYNAS 131 154698 7 (4) 7 (4) 0.06
DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
FTSZ_PSEPK 123 41918 5 (5) 5 (5) 0.31
Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
RPOB_CHRSD 115 152000 9 (5) 8 (5) 0.08
DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
RPOC_COXBN 77 157705 6 (3) 6 (3) 0.04
DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
2 samesets of RPOC_COXBN
RPOC_COXBR 77 157633 6 (3) 6 (3) 0.04
DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=rpoC PE=3 SV=1
RPOC_COXBU 77 157633 6 (3) 6 (3) 0.04
DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii GN=rpoC PE=3 SV=1

-67 peptide matches (66 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 7 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 5 6 7 Peptide
143   516.3042 515.2969 515.2955 2.79 0 8 1.7 +4Score > 47 indicates identity
Score > 23 indicates homology		 X R.ALAIE.K
250   315.2036 628.3926 628.3908 2.95 0 10 1.2 +4Score > 42 indicates identity
Score > 23 indicates homology		 U X E.AGAIVAK.W
373   347.7046 693.3946 693.3922 3.53 0 29 0.16 +1Score > 39 indicates identity
Score > 33 indicates homology		 X X X R.APTLHR.L
405   352.2226 702.4306 702.4276 4.36 0 10 2.4 +9Score > 38 indicates identity
Score > 26 indicates homology		 U X R.GLATTIK.A
428   358.7200 715.4254 715.4228 3.65 0 34 0.3 +1Score > 43 indicates identity
Score > 41 indicates homology		 U X K.TLVDIR.N
439   361.7149 721.4152 721.4122 4.16 0 20 0.25 +1Score > 39 indicates identity
Score > 26 indicates homology		 X X X K.LLYSAR  
470   367.7333 733.4520 733.4486 4.65 0 13 0.65 +1Score > 35 indicates identity
Score > 24 indicates homology		 U X K.VYLAIR.R
480   372.2569 742.4992 742.4953 5.36 0 26 0.12 +1Score > 32 indicates identity
Score > 29 indicates homology		 U X R.VLLGITK.A
498   379.2652 756.5158 756.5109 6.53 0 29 0.027 +1Score > 26 indicates identity U X K.LLTILGK.D
561   392.7390 783.4634 783.4603 4.04 0 33 0.081 +1Score > 37 indicates identity
Score > 35 indicates homology		 X R.QAVPTLR.A
734   430.7477 859.4808 859.4763 5.23 0 11 17 +10Score > 42 indicates identity
Score > 36 indicates homology		 U X R.IVDTTVGR.A
736   430.7501 859.4856 859.4803 6.17 0 27 0.1 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X K.VIDLWSK.A
756   436.2871 870.5596 870.5538 6.68 0 53 0.00066 +1Score > 33 indicates identity U X R.LLGVSALAK.Y
780   441.2476 880.4806 880.4766 4.55 1 20 0.61 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X R.HINQLEK.A
866   458.7788 915.5430 915.5389 4.52 1 65 0.00015 +1Score > 39 indicates identity U X X X R.KGLADTALK.T
875   460.7481 919.4816 919.4763 5.80 1 17 1.1 +3Score > 42 indicates identity
Score > 30 indicates homology		 U X X R.VADLFEAR.R
887   463.2275 924.4404 924.4413 -0.97 0 26 0.11 +1Score > 40 indicates identity
Score > 29 indicates homology		 X X K.NIVDGDHR.M
961   317.8636 950.5690 950.5661 2.97 0 35 0.045 +1Score > 34 indicates identity U X X X R.VIVSQLHR.S
962   318.5078 952.5016 952.4978 3.97 0 27 0.91 +2Score > 39 indicates identity X X X K.LNHLVDDK.M
963   477.7557 953.4968 953.4818 15.8 0 6 2 +9Score > 39 indicates identity
Score > 22 indicates homology		 X X X K.LNHLVDDK.M + Deamidated (NQ)
969   319.8444 956.5114 956.5080 3.56 0 38 0.0073 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X R.DVGLHAAFK.S
971   319.8680 956.5822 956.5906 -8.80 1 11 3.4 +6Score > 38 indicates identity
Score > 29 indicates homology		 U X M.KDLLNLLK.N + Deamidated (NQ)
980   480.2911 958.5676 958.5600 7.97 0 37 0.016 +1Score > 37 indicates identity
Score > 32 indicates homology		 U X R.GVTFAVPLR.V
1028   326.5234 976.5484 976.5454 3.05 1 45 0.018 +1Score > 40 indicates identity U X R.EHPVLLNR.A
1039   494.2656 986.5166 986.5185 -1.90 1 14 1.2 +2Score > 42 indicates identity
Score > 27 indicates homology		 U X R.HLNEFTVK.A
1043   494.7896 987.5646 987.5601 4.65 1 47 0.01 +1Score > 39 indicates identity U X R.VLTEAAVTGK.R
1043   494.7896 987.5646 987.5600 4.66 1 31 0.38 +4Score > 39 indicates identity U X R.VLTEASLAGK.V
1051   496.7636 991.5126 991.5087 4.00 0 40 0.076 +1Score > 41 indicates identity U X K.AQQYIVDR.R
1064   498.8344 995.6542 995.6491 5.15 1 30 0.038 +1Score > 29 indicates identity U X R.KLPASVLLR.A
1075   501.2751 1000.5356 1000.5302 5.49 0 52 0.00035 +1Score > 42 indicates identity
Score > 30 indicates homology		 U X R.ADGNLVAVSR.S
1087   503.2657 1004.5168 1004.5138 2.99 0 49 0.0021 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X R.TSAADSVQVK.N
1114   339.5152 1015.5238 1015.5199 3.79 0 24 0.55 +1Score > 42 indicates identity
Score > 34 indicates homology		 U X X R.TFHIGGAASR  
1123   510.3073 1018.6000 1018.6175 -17.1 1 9 12 +4Score > 37 indicates identity
Score > 32 indicates homology		 U X X K.VRLIIYDK.D
1127   511.2733 1020.5320 1020.5240 7.88 1 55 0.0027 +1Score > 42 indicates identity
Score > 41 indicates homology		 U X R.IVQGATFER.L + Deamidated (NQ)
1136   514.3013 1026.5880 1026.5822 5.70 0 52 0.0027 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X X X R.VSALGPGGLTR.E
1169   521.8205 1041.6264 1041.6183 7.86 0 32 0.07 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X R.SVITVGPTLR.L
1183   527.8223 1053.6300 1053.6182 11.2 1 33 0.024 +1Score > 35 indicates identity
Score > 29 indicates homology		 U X K.LSLELVPQR.L
1248   543.8024 1085.5902 1085.5903 -0.035 1 31 0.087 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.IGLASPEMIR.S
1254   363.2257 1086.6553 1086.6471 7.55 1 30 0.13 +1Score > 34 indicates identity U X -.MKDLLNLLK.N
1267   549.2902 1096.5658 1096.5625 3.04 1 48 0.0086 +1Score > 40 indicates identity U X K.GLGAGANPEVGR.Q
1325   565.8444 1129.6742 1129.6707 3.15 0 42 0.0012 +1Score > 34 indicates identity
Score > 26 indicates homology		 U X R.TILQLGTGVTK.G
1394 +1 578.8217 1155.6288 1155.6499 -18.2 1 68 3e-005 +1Score > 40 indicates identity
Score > 35 indicates homology		 U X K.QLIDELVAVR.H + Deamidated (NQ)
1442   392.8540 1175.5402 1175.5360 3.55 0 48 0.00037 +1Score > 41 indicates identity
Score > 26 indicates homology		 U X R.SPGVFFDHDR.G
1479   596.3066 1190.5986 1190.5932 4.61 1 41 0.014 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X R.VFADLQEVDR.V
1582   617.8484 1233.6822 1233.6718 8.49 0 48 0.00096 +1Score > 39 indicates identity
Score > 30 indicates homology		 U X X X K.GTVIDVQVFTR.D
1737   657.8260 1313.6374 1313.6252 9.35 0 43 0.002 +1Score > 40 indicates identity
Score > 28 indicates homology		 U X X X R.FATSDLNDLYR.R
1765   441.8961 1322.6665 1322.6579 6.49 0 54 0.00014 +1Score > 41 indicates identity
Score > 28 indicates homology		 U X K.GIVDDIDHLGNR.R
1767   662.9037 1323.7928 1323.7762 12.6 0 58 0.00013 +1Score > 32 indicates identity U X R.LLDLSAPDIIVR.N
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.raw

Score > 40 indicates identity
1946   706.3705 1410.7264 1410.7143 8.60 1 69 7.2e-005 -1Score > 40 indicates identity U X K.YIVNEIQDVYR.L
8.60 1 44 0.021 2 YLVNEVQQVYR   + Deamidated (NQ)
8.60 1 44 0.021 2 YLVNQVQEVYR   + Deamidated (NQ)
8.60 1 44 0.023 4 YLVNQVQEVYR   + Deamidated (NQ)
8.60 1 38 0.086 5 YLVNEVQQVYR   + Deamidated (NQ)
8.60 1 30 0.53 6 YLVNQVQEVYR   + Deamidated (NQ)
-16.2 1 22 3.7 7 LPLQDLLELVQE   + 2 Deamidated (NQ)
8.60 1 22 3.9 8 YIINEVQDVYR  
19.4 0 14 26 9 HLDSVLQQLQTE   + Deamidated (NQ)
-14.3 1 13 30 10 IDQINNLIPESR  
2064   737.8540 1473.6934 1473.6888 3.13 1 73 3e-005 +1Score > 40 indicates identity U X R.TNQYGFLESPYR.V
2085   495.6021 1483.7845 1483.7783 4.16 1 75 8.7e-006 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X R.LIPAGTGLAYHSER.K
2260   781.8836 1561.7526 1561.7592 -4.21 1 3 1.5 +7Score > 41 indicates identity
Score > 18 indicates homology		 U X R.QLAGMRGLMAKPSGE.I + Deamidated (NQ); Oxidation (M)
2283   393.9613 1571.8161 1571.8096 4.11 1 12 0.12 +1Score > 40 indicates identity
Score > 15 indicates homology		 U X K.WDPHTHPIVTELK.G
2415   811.9260 1621.8374 1621.8312 3.88 0 88 5.5e-007 +1Score > 40 indicates identity
Score > 38 indicates homology		 U X X X R.STGSYSLVTQQPLGGK.A
2556   565.6545 1693.9417 1693.9185 13.7 1 35 0.036 +1Score > 35 indicates identity
Score > 33 indicates homology		 U X R.MNVGQILETHLGLAAK.G
2654   874.4460 1746.8774 1746.8611 9.38 1 91 5.2e-007 +1Score > 41 indicates identity U X K.LADLPESGQMVLFDGR.T
2715   593.3223 1776.9451 1776.9159 16.4 1 43 0.00086 +1Score > 39 indicates identity
Score > 25 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
3096   1009.5313 2017.0480 2017.0156 16.1 0 85 1.6e-007 +1Score > 38 indicates identity
Score > 29 indicates homology		 U X K.LNPQDDLDYLDIPAFLR.R
3117   1017.5043 2032.9940 2032.9589 17.3 1 87 5.2e-007 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X K.ASLSTQSFISAASFQETTR.V + 2 Deamidated (NQ)
3368   1083.0741 2164.1336 2164.1277 2.77 1 24 0.049 +1Score > 37 indicates identity
Score > 24 indicates homology		 U X R.QVVSVAAALIPFLEHDDANR.A
3406   727.7262 2180.1568 2180.1226 15.7 1 31 0.0033 +1Score > 37 indicates identity
Score > 19 indicates homology		 U X X QVVSVAASLIPFLEHDDANR.A
3430   730.3931 2188.1575 2188.1310 12.1 1 24 0.0075 +1Score > 37 indicates identity
Score > 15 indicates homology		 U X R.GLMARPDGTIIETPITANFR.E + Oxidation (M)
3473   736.0518 2205.1336 2205.1106 10.4 1 4 1.1 +2Score > 38 indicates identity
Score > 17 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L
3474   1103.5767 2205.1388 2205.1106 12.8 1 109 1e-010 +1Score > 38 indicates identity
Score > 21 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L
3740   791.4050 2371.1932 2371.1768 6.91 1 33 0.0041 +1Score > 38 indicates identity
Score > 21 indicates homology		 U X K.VVDNTLQTAQQAYEASNPAPVR.Q

+65 subsets and intersections (987 subset proteins in total)

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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