MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 240)


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+81

Accession Score Description
1 NDK_PSEFS 74 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1

+82

Accession Score Description
1 ODBB_PSEPU 72 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+83

Accession Score Description
1 RRF_ACTSZ 72 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+84

Accession Score Description
1 GLPD_PSETO 72 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

+85

Accession Score Description
1 ATPE_PSEPG 70 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+86

Accession Score Description
1 RL6_PSEP1 69 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+87

Accession Score Description
1 RL20_PSEE4 68 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

+88

Accession Score Description
1 RL15_PSEE4 67 50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

-89

Accession Score Description
1 RL10_PSEPG 66 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
Score Mass Matches Sequences emPAI
89.1 RL10_PSEPG 66 17676 5 (3) 5 (3) 0.46
50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
2 samesets of RL10_PSEPG
RL10_PSEPK 66 17676 5 (3) 5 (3) 0.46
50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1
RL10_PSEPW 66 17648 4 (3) 4 (3) 0.46
50S ribosomal protein L10 OS=Pseudomonas putida (strain W619) GN=rplJ PE=3 SV=1

+5 peptide matches (5 non-duplicate, 0 duplicate)

3 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
RL10_PSEE4 57 17602 89.1
50S ribosomal protein L10 OS=Pseudomonas entomophila (strain L48) GN=rplJ PE=3 SV=1
RL10_AZOVD 47 17534 89.1
50S ribosomal protein L10 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplJ PE=3 SV=1
6 samesets of RL10_AZOVD
RL10_PSEA7 47 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain PA7) GN=rplJ PE=3 SV=1
RL10_PSEA8 47 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplJ PE=3 SV=1
RL10_PSEAB 47 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplJ PE=3 SV=1
RL10_PSEAE 47 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa GN=rplJ PE=3 SV=1
RL10_PSEMY 47 17574
50S ribosomal protein L10 OS=Pseudomonas mendocina (strain ymp) GN=rplJ PE=3 SV=1
RL10_PSEU5 47 17514
50S ribosomal protein L10 OS=Pseudomonas stutzeri (strain A1501) GN=rplJ PE=3 SV=1
RL10_MARMS 39 17773 89.1
50S ribosomal protein L10 OS=Marinomonas sp. (strain MWYL1) GN=rplJ PE=3 SV=1
2 samesets of RL10_MARMS
RL10_PSEF5 39 17619
50S ribosomal protein L10 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplJ PE=3 SV=1
RL10_PSEPF 39 17549
50S ribosomal protein L10 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplJ PE=3 SV=1

+90

Accession Score Description
1 GRPE_PSEPK 65 Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1
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