MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 240)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 24 Next 

+81

Accession Score Description
1 NDK_PSEFS 74 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1

+82

Accession Score Description
1 ODBB_PSEPU 72 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+83

Accession Score Description
1 RRF_ACTSZ 72 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+84

Accession Score Description
1 GLPD_PSETO 72 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

+85

Accession Score Description
1 ATPE_PSEPG 70 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+86

Accession Score Description
1 RL6_PSEP1 69 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

-87

Accession Score Description
1 RL20_PSEE4 68 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
Score Mass Matches Sequences emPAI
87.1 RL20_PSEE4 68 13272 11 (4) 8 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
13 samesets of RL20_PSEE4
RL20_PSEP1 68 13272 11 (4) 8 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplT PE=3 SV=1
RL20_PSEPG 68 13272 11 (4) 8 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas putida (strain GB-1) GN=rplT PE=3 SV=1
RL20_PSEPK 68 13272 11 (4) 8 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
RL20_PSEPW 68 13272 11 (4) 8 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas putida (strain W619) GN=rplT PE=3 SV=1
RL20_PSEF5 68 13287 10 (4) 7 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplT PE=3 SV=1
RL20_PSEFL 68 13287 10 (4) 7 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas fluorescens GN=rplT PE=3 SV=1
RL20_PSEMY 68 13241 10 (4) 7 (2) 0.65
50S ribosomal protein L20 OS=Pseudomonas mendocina (strain ymp) GN=rplT PE=3 SV=1
RL20_PSEU5 68 13284 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas stutzeri (strain A1501) GN=rplT PE=3 SV=1
RL20_PSE14 68 13306 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplT PE=3 SV=1
RL20_PSEPF 68 13306 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplT PE=3 SV=1
RL20_PSESM 68 13306 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas syringae pv. tomato GN=rplT PE=3 SV=1
RL20_PSESY 68 13306 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae GN=rplT PE=3 SV=1
RL20_PSEU2 68 13306 9 (4) 6 (2) 0.64
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplT PE=3 SV=1

-11 peptide matches (9 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
221   307.7164 613.4182 613.4163 3.22 0 29 0.29 +1Score > 36 indicates identity R.LIAGLK.K
354   686.3288 685.3215 685.3184 4.62 0 13 1.2 +2Score > 37 indicates identity
Score > 27 indicates homology		 K.GYYGAR.S
454   365.2255 728.4364 728.4333 4.30 0 25 1.3 +4Score > 41 indicates identity
Score > 39 indicates homology		 R.ALWIAR.I
616   402.2177 802.4208 802.4185 2.98 1 25 0.78 +1Score > 44 indicates identity
Score > 36 indicates homology		 K.ASIEIDR.K
655   414.7050 827.3954 827.3926 3.47 0 28 0.065 +1Score > 38 indicates identity
Score > 29 indicates homology		 K.AGQYAYR.D
812   449.2266 896.4386 896.4352 3.87 0 23 0.44 +1Score > 40 indicates identity
Score > 32 indicates homology		 U R.TNGLSYSR.L
871   460.2698 918.5250 918.5174 8.28 1 40 0.0017 +1Score > 38 indicates identity
Score > 25 indicates homology		 U K.AAFAAIVEK.A
872   919.5331 918.5258 918.5174 9.13 1 45 0.012 +1Score > 38 indicates identity U K.AAFAAIVEK.A
1214 +2 536.3095 1070.6044 1070.5972 6.81 1 47 0.011 +1Score > 40 indicates identity U K.VLADLAVNEK.A

3 subsets and intersections (9 subset proteins in total)

Score Mass Subset of
RL20_AZOVD 53 13385 87.1
50S ribosomal protein L20 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplT PE=3 SV=1
2 samesets of RL20_AZOVD
RL20_AZOVI 53 13429
50S ribosomal protein L20 OS=Azotobacter vinelandii GN=rplT PE=3 SV=1
RL20_PROMH 53 13449
50S ribosomal protein L20 OS=Proteus mirabilis (strain HI4320) GN=rplT PE=3 SV=1
RL20_POLNS 53 13738 87.1
50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
1 sameset of RL20_POLNS
RL20_POLSQ 53 13742
50S ribosomal protein L20 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rplT PE=3 SV=1
RL20_PSEA7 50 13357 87.1
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain PA7) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEA7
RL20_PSEA8 50 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplT PE=3 SV=1
RL20_PSEAB 50 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplT PE=3 SV=1
RL20_PSEAE 50 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa GN=rplT PE=3 SV=1

+88

Accession Score Description
1 RL15_PSEE4 67 50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

+89

Accession Score Description
1 RL10_PSEPG 66 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+90

Accession Score Description
1 GRPE_PSEPK 65 Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1
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