Rita5 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita5 sp
MS data file	:	PRT1270_T-BRSC_5_20250714121805.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:42:02 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita5 sp

MS data file

PRT1270_T-BRSC_5_20250714121805.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:42:02 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,997

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,997

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Protein families 81–90 (out of 240)

Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 24 Next

81

		Accession	Score	Description
	1	NDK_PSEFS	74	Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1

82

		Accession	Score	Description
	1	ODBB_PSEPU	72	2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

83

		Accession	Score	Description
	1	RRF_ACTSZ	72	Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

84

		Accession	Score	Description
	1	GLPD_PSETO	72	Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

85

		Accession	Score	Description
	1	ATPE_PSEPG	70	ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

86

		Accession	Score	Description
	1	RL6_PSEP1	69	50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
86.1	RL6_PSEP1	69	19131	5 (2)	5 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
	7 samesets of RL6_PSEP1
	RL6_PSEPG	69	19131	5 (2)	5 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas putida (strain GB-1) GN=rplF PE=3 SV=1
	RL6_PSEPK	69	19131	5 (2)	5 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1
	RL6_PSEF5	69	19170	4 (2)	4 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplF PE=3 SV=1
	RL6_PSEFS	69	19185	4 (2)	4 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain SBW25) GN=rplF PE=3 SV=1
	RL6_PSEPF	69	19184	4 (2)	4 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplF PE=3 SV=1
	RL6_PSEPW	69	19115	4 (2)	4 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas putida (strain W619) GN=rplF PE=3 SV=1
	RL6_PSEE4	69	19015	3 (2)	3 (2)	0.26
	50S ribosomal protein L6 OS=Pseudomonas entomophila (strain L48) GN=rplF PE=3 SV=1

5 peptide matches (5 non-duplicate, 0 duplicate)

Query	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
656	414.7472	827.4798	827.4753	5.54	1	24	1.4	2Score > 40 indicates identity Score > 38 indicates homology		K.LPSGVEVK.F
702	426.2180	850.4214	850.4185	3.50	1	7	0.81	1Score > 42 indicates identity Score > 19 indicates homology		R.YADEVVR.R
1027	489.2764	976.5382	976.5342	4.17	0	46	0.0068	1Score > 41 indicates identity Score > 37 indicates homology	U	K.FAGQQLSVK.G
1276	552.8464	1103.6782	1103.6703	7.23	1	57	0.00013	1Score > 30 indicates identity	U	R.KLQLVGVGYK.A
1376	383.5307	1147.5703	1147.5662	3.55	0	17	0.17	1Score > 42 indicates identity Score > 22 indicates homology	U	R.DFRPPEPYK.G

1 subset or intersection (16 subset proteins in total)

	Score	Mass	Subset of
RL6_ACIB3	57	19085	86.1
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplF PE=3 SV=1
15 samesets of RL6_ACIB3
RL6_PSE14	57	19506
50S ribosomal protein L6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplF PE=3 SV=1
RL6_PSESM	57	19266
50S ribosomal protein L6 OS=Pseudomonas syringae pv. tomato GN=rplF PE=3 SV=1
RL6_PSEU2	57	19236
50S ribosomal protein L6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplF PE=3 SV=1
RL6_PSEA7	57	19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain PA7) GN=rplF PE=3 SV=1
RL6_PSEA8	57	19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplF PE=3 SV=1
RL6_PSEAB	57	19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplF PE=3 SV=1
RL6_PSEAE	57	19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa GN=rplF PE=3 SV=1
RL6_PSEMY	57	19130
50S ribosomal protein L6 OS=Pseudomonas mendocina (strain ymp) GN=rplF PE=3 SV=1
RL6_ACIB5	57	19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB0057) GN=rplF PE=3 SV=1
RL6_ACIBS	57	19113
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain SDF) GN=rplF PE=3 SV=1
RL6_ACIBT	57	19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=rplF PE=3 SV=2
RL6_ACIBY	57	19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AYE) GN=rplF PE=3 SV=1
RL6_AERHH	57	18568
50S ribosomal protein L6 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplF PE=3 SV=1
RL6_AERS4	57	18556
50S ribosomal protein L6 OS=Aeromonas salmonicida (strain A449) GN=rplF PE=3 SV=1
RL6_PSEU5	57	19167
50S ribosomal protein L6 OS=Pseudomonas stutzeri (strain A1501) GN=rplF PE=3 SV=1

87

		Accession	Score	Description
	1	RL20_PSEE4	68	50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

88

		Accession	Score	Description
	1	RL15_PSEE4	67	50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

89

		Accession	Score	Description
	1	RL10_PSEPG	66	50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

90

		Accession	Score	Description
	1	GRPE_PSEPK	65	Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1

Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 24 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 81–90 (out of 240)

81

82

83

84

85

86

5 peptide matches (5 non-duplicate, 0 duplicate)

1 subset or intersection (16 subset proteins in total)

87

88

89

90