MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 240)


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+81

Accession Score Description
1 NDK_PSEFS 74 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1

+82

Accession Score Description
1 ODBB_PSEPU 72 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+83

Accession Score Description
1 RRF_ACTSZ 72 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+84

Accession Score Description
1 GLPD_PSETO 72 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

-85

Accession Score Description
1 ATPE_PSEPG 70 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
Score Mass Matches Sequences emPAI
85.1 ATPE_PSEPG 70 14606 4 (2) 3 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
2 samesets of ATPE_PSEPG
ATPE_PSEPK 70 14585 4 (2) 3 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1
ATPE_PSEPW 70 14595 3 (2) 2 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain W619) GN=atpC PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
932 +1 471.7747 941.5348 941.5294 5.80 1 52 0.0032 +1Score > 39 indicates identity U R.LAEAAAQLR.T
936   472.2670 942.5194 942.5134 6.42 1 26 0.23 +1Score > 41 indicates identity
Score > 33 indicates homology
U K.AAENALNLK.G
1461   592.7719 1183.5292 1183.5258 2.92 0 57 0.0008 +1Score > 39 indicates identity
Score > 39 indicates homology
U K.GADFDYGAAAAR.L

1 subset or intersection (20 subset proteins in total)

Score Mass Subset of
ATPE_COXB1 52 15277 85.1
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuK_Q154) GN=atpC PE=3 SV=1
19 samesets of ATPE_COXB1
ATPE_PSEP1 52 14636
ATP synthase epsilon chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpC PE=3 SV=1
ATPE_MARAV 52 15117
ATP synthase epsilon chain OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=atpC PE=3 SV=1
ATPE_COXBN 52 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain Dugway 5J108-111) GN=atpC PE=3 SV=1
ATPE_COXBR 52 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=atpC PE=3 SV=1
ATPE_COXBU 52 15277
ATP synthase epsilon chain OS=Coxiella burnetii GN=atpC PE=3 SV=1
ATPE_PSEAB 52 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpC PE=3 SV=1
ATPE_PSEAE 52 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa GN=atpC PE=3 SV=1
ATPE_PSEFS 52 14615
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpC PE=3 SV=1
ATPE_PSEMY 52 15370
ATP synthase epsilon chain OS=Pseudomonas mendocina (strain ymp) GN=atpC PE=3 SV=1
ATPE_PSEPF 52 14802
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpC PE=3 SV=1
ATPE_PSESM 52 14607
ATP synthase epsilon chain OS=Pseudomonas syringae pv. tomato GN=atpC PE=3 SV=1
ATPE_TERTT 52 14938
ATP synthase epsilon chain OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=atpC PE=3 SV=1
ATPE_COXB2 52 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuG_Q212) GN=atpC PE=3 SV=1
ATPE_PSEU2 52 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpC PE=3 SV=1
ATPE_PSEE4 52 14635
ATP synthase epsilon chain OS=Pseudomonas entomophila (strain L48) GN=atpC PE=3 SV=1
ATPE_PSEF5 52 14853
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpC PE=3 SV=1
ATPE_PSEA7 52 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpC PE=3 SV=1
ATPE_PSEA8 52 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpC PE=3 SV=1
ATPE_PSE14 52 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpC PE=3 SV=1

+86

Accession Score Description
1 RL6_PSEP1 69 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+87

Accession Score Description
1 RL20_PSEE4 68 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

+88

Accession Score Description
1 RL15_PSEE4 67 50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

+89

Accession Score Description
1 RL10_PSEPG 66 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+90

Accession Score Description
1 GRPE_PSEPK 65 Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1
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